The main goal of this research was to introgressed the genetic male sterility (GMS) gene ms10 from hot pepper (Capsicum annuum var. annuum) to heat tolerant bell pepper (C. annuum var. grossum) inbreds and to pyramid it with the non-pungent (pun1) gene. The gene positive plant selection was assisted by the SSR marker linked to ms10 gene located on chromosome I and the SCAR marker indicating the non-pungent pun1 gene presence. First, we backcrossed and screened BC₁F₁ and BC₂F₁ plants having heterozygous (Ms10ms10) genotype for GMS specific marker AVRDC-PP12. Secondly, the background recovery rate of the recurrent parent phenome (RPP) in both the backcrossed progeny was estimated by measuring the shift in means for fruit traits. Simultaneously, targeted gene positive (Ms10ms10) non-pungent (pun1) heterozygous plant(s) were identified in BC₂F₁ generation using BF7 and BF 9-SCAR marker. The best gene positive individual plant from each population was backcrossed with their respective recurrent parent and selfed for validation of ms10 gene. Finally, we obtained six gene positive (ms10) GMS lines designated as MSSM-1, MSSM-2, MSSM-3, MSSM-9, MSSM-17, and MSSM-21 from this work. To the best of our knowledge, this is the first time that GMS ms10 gene has been successfully transferred from hot pepper to heat tolerant bell pepper through marker-assisted backcross breeding scheme coupled with stringent phenotypic selection. The developed GMS lines will provide great economic value for non-pungent hybrid bell pepper seed production in the near future.

这项研究的主要目标是渗入遗传雄性不育（GMS）基因MS10从辣椒（辣椒变种辣椒），以耐热的甜椒（辣椒变种grossum）自交系，并与非到金字塔它pungent（pun1）基因。基因阳性植物的选择是通过与位于I号染色体上的ms10基因相连的SSR标记和表明非刺激性pun1基因存在的SCAR标记来辅助的。首先，我们回交并筛选BC ₁ ˚F ₁和BC ₂ ˚F _1种具有杂合的植物（Ms10ms10GMS特异性标记AVRDC-PP12的基因型）。其次，通过测量果实性状均值的变化，估算了两个回交后代中轮回亲本现象（RPP）的背景恢复率。同时，使用BF7和BF 9-SCAR标记在BC ₂ F ₁世代中鉴定了目标基因阳性（Ms10ms10）非刺激性（pun1）杂合植物。将来自每个群体的最佳基因阳性个体植物与它们各自的轮回亲本回交并自交以验证ms10基因。最后，我们获得了六个基因阳性（ms10）根据这项工作，指定为MSSM-1，MSSM-2，MSSM-3，MSSM-9，MSSM-17和MSSM-21的GMS线路。据我们所知，这是GMS ms10基因首次通过标记辅助回交育种方案和严格的表型选择成功地从辣椒转移到耐热性灯笼椒。所开发的GMS品系将在不久的将来为非辛辣型甜椒种子生产提供巨大的经济价值。