Abstract

Biobased chemicals are gaining popularity and market in attempts to mitigate the deteriorating environmental and sustainability issues. Components of renewable agricultural and forest biomass residues are projected to serve as abundant precursors to synthesis of expanding range of products. Agroindustrial wastes comprises of several phenolic compounds associated with lignin via ether linkages such as ferulic acid, p-coumaric, syringic acid and vanillin. These aromatic chemicals have myriad industrial applications. In this study, p-coumaric acid and ferulic acid were found to be two major components in corn bran derived lignin hydrolysate. Engineered Pseudomonas putida KT2440 was constructed and found to convert p-coumaric acid and vanillic acid to protocatechuic acid in >90% and >50% yields, respectively. Engineering the strain included deletion of the gene encoding protocatechuate 3,4-dioxygenase, and overexpression of vanillate-O-demethylase gene from Acinetobacter sp. ADP1.

摘要

生物基化学品越来越受欢迎，并试图缓解日益恶化的环境和可持续性问题。可再生农业和森林生物质残留物的成分预计将作为丰富的前体，用于合成不断扩大的产品范围。农工业废料包括通过醚键与木质素结合的几种酚类化合物，例如阿魏酸、对香豆酸、丁香酸和香草醛。这些芳香族化学品有无数的工业应用。在这项研究中，发现对香豆酸和阿魏酸是玉米糠衍生的木质素水解物中的两种主要成分。工程恶臭假单胞菌构建 KT2440 并发现其可分别以 >90% 和 >50% 的产率将对香豆酸和香草酸转化为原儿茶酸。对菌株进行改造包括删除编码原儿茶酸 3,4-双加氧酶的基因，以及来自不动杆菌属的香草酸-O-脱甲基酶基因的过表达。ADP1。