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Trends in lipase engineering for enhanced biocatalysis
Biotechnology and Applied Biochemistry ( IF 3.2 ) Pub Date : 2021-01-13 , DOI: 10.1002/bab.2105 Surabhi Soni 1
Biotechnology and Applied Biochemistry ( IF 3.2 ) Pub Date : 2021-01-13 , DOI: 10.1002/bab.2105 Surabhi Soni 1
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Lipases, also known as triacylglycerol hydrolases (E.C.No. 3.1.1.3), are considered as leading biocatalysts in the lipid modification business. With properties like ease of availability, capability to work in heterogeneous media, stability in organic solvents, property of catalyzing at the lipid–water interface and even in nonaqueous conditions, have made them a versatile choice for applications in the food, flavor, detergent, pharmaceutical, leather, textile, cosmetic, and paper industries [1]. The increasing alertness toward sustainable technologies, lesser waste generation and solvent usage and minimization of energy input has brought light toward the production and usage of recombinant/improved lipases. For example, Novozym 435, a broadly used recombinant lipase isolated from Candida antarctica, dominates the lipase industry and has even created a supplier bias in the market. This shows that there is a desperate need for novel, low-cost lipases with better properties. For this, mining of existing extremophilic genomes seems more rewarding. But considering the diversity of industrial requirements such as types of solvents used or carrier systems employed for enzyme immobilization, tailor-designed enzymes are an unrealized pressing priority. Therefore, protein engineering strategies in collaboration with the discovery of new lipases can serve as a vital tool to obtain tailor-made enzymes with specific characteristics.
中文翻译：
用于增强生物催化的脂肪酶工程趋势
脂肪酶，也称为三酰基甘油水解酶（ECNo. 3.1.1.3），被认为是脂质修饰业务中领先的生物催化剂。具有易于获得、在非均相介质中工作的能力、在有机溶剂中的稳定性、在脂质-水界面甚至在非水条件下催化的特性等特性，使其成为食品、香料、洗涤剂、制药、皮革、纺织、化妆品和造纸工业 [1]。对可持续技术的日益警觉、更少的废物产生和溶剂使用以及能量输入的最小化为重组/改良脂肪酶的生产和使用带来了光明。例如，Novozym 435，一种广泛使用的从南极念珠菌中分离的重组脂肪酶，主导脂肪酶行业，甚至在市场上造成了供应商偏见。这表明迫切需要具有更好性能的新型低成本脂肪酶。为此，挖掘现有的嗜极基因组似乎更有价值。但考虑到工业要求的多样性，例如用于酶固定的溶剂类型或载体系统，量身定制的酶是一个未实现的紧迫优先事项。因此，蛋白质工程策略与新脂肪酶的发现相结合，可以作为获得具有特定特性的定制酶的重要工具。
更新日期：2021-01-13
中文翻译：
用于增强生物催化的脂肪酶工程趋势
脂肪酶，也称为三酰基甘油水解酶（ECNo. 3.1.1.3），被认为是脂质修饰业务中领先的生物催化剂。具有易于获得、在非均相介质中工作的能力、在有机溶剂中的稳定性、在脂质-水界面甚至在非水条件下催化的特性等特性，使其成为食品、香料、洗涤剂、制药、皮革、纺织、化妆品和造纸工业 [1]。对可持续技术的日益警觉、更少的废物产生和溶剂使用以及能量输入的最小化为重组/改良脂肪酶的生产和使用带来了光明。例如，Novozym 435，一种广泛使用的从南极念珠菌中分离的重组脂肪酶，主导脂肪酶行业，甚至在市场上造成了供应商偏见。这表明迫切需要具有更好性能的新型低成本脂肪酶。为此，挖掘现有的嗜极基因组似乎更有价值。但考虑到工业要求的多样性，例如用于酶固定的溶剂类型或载体系统，量身定制的酶是一个未实现的紧迫优先事项。因此，蛋白质工程策略与新脂肪酶的发现相结合，可以作为获得具有特定特性的定制酶的重要工具。
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