Curtobacterium flaccumfaciens pv. flaccumfaciens (Cff) is a bacterial vascular pathogen that infects economically important crops, such as soybean and beans. Currently, this pathogen is widespread in the main bean-producing regions, causing great economic losses. Considering the environmental adaptability of this pathogen and its evolution over time, it is necessary to obtain new information and analyse its status. The objective of this study was to identify whether Cff occurs in the field by collecting plants from different regions in the state of Paraná and determine its pathogenicity in bean plants. For Cff diagnosis, plants with wilt symptoms were obtained from commercial and experimental crops in the Campo do Tenente, Corbélia, Ivaí, Santa Tereza do Oeste, and Sengés municipalities. The samples were subjected to a bacteriological examination and isolation of the bacteria in specific culture medium. To confirm the species of the isolates obtained, specific primers for Cff were used in PCR. The isolates were inoculated into susceptible bean plants (BRS Supremo) to evaluate their pathogenicity. The experimental design adopted was a randomized block design with four replicates. The bacterium was found in all collection locations. Sixteen isolates were characterized as Cff by PCR. The isolates inoculated into the susceptible bean cultivar showed high levels of severity. The results show that Cff isolates in bean crops in bean-producing regions in Paraná show a certain level of field adaptability.

flaccumfaciens pv。黄曲霉（Cff）是一种细菌性血管病原体，可感染具有经济意义的农作物，例如大豆和豆类。目前，这种病原体在主要的豆类产区广泛分布，造成了巨大的经济损失。考虑到该病原体的环境适应性及其随时间的演变，有必要获取新信息并分析其状况。这项研究的目的是通过收集巴拉那州不同地区的植物来鉴定Cff是否在田间发生，并确定其在豆类植物中的致病性。对于Cff诊断，从Campo do Tenente，Corbélia，Ivaí，Santa Tereza do Oeste和Sengés市的商业和实验作物中获得了带有枯萎症状的植物。对样品进行细菌学检查，并在特定培养基中分离细菌。为了确认获得的分离物的种类，将Cff的特异性引物用于PCR。将分离株接种到易感的豆类植物（BRS Supremo）中以评估其致病性。采用的实验设计是具有四个重复的随机区组设计。在所有收集地点都发现了该细菌。通过PCR将16个分离物表征为Cff。接种到易感大豆品种中的分离株显示出高水平的严重性。结果表明，巴拉那州豆类产区豆类作物的Cff分离株具有一定的田间适应性。将分离株接种到易感的豆类植物（BRS Supremo）中以评估其致病性。采用的实验设计是具有四个重复的随机区组设计。在所有收集地点都发现了该细菌。通过PCR将16个分离物表征为Cff。接种到易感大豆品种中的分离株显示出高水平的严重性。结果表明，巴拉那州豆类产区豆类作物的Cff分离株具有一定的田间适应性。将分离株接种到易感的豆类植物（BRS Supremo）中以评估其致病性。采用的实验设计是具有四个重复的随机区组设计。在所有收集地点都发现了该细菌。通过PCR将16个分离物表征为Cff。接种到易感大豆品种中的分离株显示出高水平的严重性。结果表明，巴拉那州豆类产区豆类作物的Cff分离株具有一定的田间适应性。