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Low frequency vortex magnetic field reduces amyloid β aggregation, increase cell viability and protect from amyloid β toxicity
Electromagnetic Biology and Medicine ( IF 1.7 ) Pub Date : 2020-10-12 , DOI: 10.1080/15368378.2020.1830288
Alejandro Maldonado-Moreles 1 , Teodoro Cordova-Fraga 2 , Herlinda Bonilla-Jaime 3 , Perla Y Lopez-Camacho 4 , Gustavo Basurto-Islas 2
Affiliation  

Plaques formed by abnormal accumulation of amyloid β-peptide (Aβ) lead to onset of Alzheimer's disease (AD). Pharmacological treatments do not reduce Aβ aggregation neither restore learning and memory. Noninvasive techniques have emerged as an alternative to treat AD, such as stimulation with electromagnetic fields (EMF) that decrease Aβ deposition and reverses cognitive impairment in AD mice, even though some studies showed side effects on parallel magnetic fields stimulation. As a new approach of magnetic field (MF) stimulation, vortex magnetic fields (VMF) have been tested inducing a random movement of charged biomolecules in cells, promoting cell viability and apparently safer than parallel magnetic fields. In this study we demonstrate the effect of VMF on Aβ aggregation. The experimental strategy includes, i) design and construction of a coil capable to induce VMF, ii) evaluation of VMF stimulation on Aβ peptide induced-fibrils-formation, iii) evaluation of VMF stimulation on SH-SY5Y neuroblastoma cell line in the presence of Aβ peptide. We demonstrated for the first time that Aβ aggregation exposed to VMF during 24 h decreased ~ 86% of Aβ fibril formation compared to control. Likewise, VMF stimulation reduced Aβ fibrils-cytotoxicity and increase SH-SY5Y cell viability. These data establish the basis for future investigation that involve VMF as inhibitor of Aβ-pathology and indicate the therapeutic potential of VMF for AD treatment.

中文翻译:

低频涡旋磁场减少淀粉样蛋白 β 聚集,增加细胞活力并防止淀粉样蛋白 β 毒性

由淀粉样蛋白 β-肽 (Aβ) 异常积累形成的斑块导致阿尔茨海默病 (AD) 的发作。药物治疗不会减少 Aβ 聚集,也不会恢复学习和记忆。无创技术已成为治疗 AD 的替代方法,例如电磁场 (EMF) 刺激可减少 Aβ 沉积并逆转 AD 小鼠的认知障碍,尽管一些研究表明平行磁场刺激会产生副作用。作为磁场 (MF) 刺激的一种新方法,涡旋磁场 (VMF) 已被测试诱导细胞中带电生物分子的随机运动,促进细胞活力,并且显然比平行磁场更安全。在这项研究中,我们证明了 VMF 对 Aβ 聚集的影响。实验策略包括,i) 能够诱导 VMF 的线圈的设计和构建,ii) 评估 VMF 对 Aβ 肽诱导的原纤维形成的刺激,iii) 在存在 Aβ 肽的情况下评估对 SH-SY5Y 神经母细胞瘤细胞系的 VMF 刺激。我们首次证明,与对照相比,在 24 小时内暴露于 VMF 的 Aβ 聚集减少了约 86% 的 Aβ 原纤维形成。同样,VMF 刺激降低了 Aβ 原纤维的细胞毒性并增加了 SH-SY5Y 细胞的活力。这些数据为将来涉及 VMF 作为 Aβ 病理学抑制剂的研究奠定了基础,并表明 VMF 对 AD 治疗的治疗潜力。我们首次证明,与对照相比,在 24 小时内暴露于 VMF 的 Aβ 聚集减少了约 86% 的 Aβ 原纤维形成。同样,VMF 刺激降低了 Aβ 原纤维的细胞毒性并增加了 SH-SY5Y 细胞的活力。这些数据为将来涉及 VMF 作为 Aβ 病理学抑制剂的研究奠定了基础,并表明 VMF 对 AD 治疗的治疗潜力。我们首次证明,与对照相比,在 24 小时内暴露于 VMF 的 Aβ 聚集减少了约 86% 的 Aβ 原纤维形成。同样,VMF 刺激降低了 Aβ 原纤维的细胞毒性并增加了 SH-SY5Y 细胞的活力。这些数据为将来涉及 VMF 作为 Aβ 病理学抑制剂的研究奠定了基础,并表明 VMF 对 AD 治疗的治疗潜力。
更新日期:2020-10-12
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