The potential use of alternative culture media towards the development of a sustainable bioprocess to produce lipases by Diutina rugosa is clearly demonstrated. First, a synthetic medium containing glucose, peptone, yeast extract, oleic acid, and ammonium sulfate was proposed, with lipase activity of 143 U/L. Then, alternative culture media formulated with agro-industrial residues, such as molasses, corn steep liquor (CSL), and olive mill waste (OMW), were investigated. An experimental design was conducted, and only CSL concentration was found to have a positive effect in lipase production. The highest lipase activity (561 U/L) was produced on a mixture of molasses (5 g/L), CSL (6 g/L), OMW (0.5% v/v), 0.5 g/L of ammonium sulfate, and 3 g/L of peptone at 24 h of cultivation. Lipase production was also carried out in a 1-L bioreactor leading to a slightly higher lipase activity at 24 h of cultivation. The semi-purified enzyme exhibits an optimum temperature and pH of 40 °C and 7.0, respectively. Finally, the media cost per unit of lipase produced (UPC) was influenced by the medium components, specially by the inducer used. The lowest UPC was obtained when the agro-industrial residues were combined and used at the improved concentrations.

清楚地证明了替代培养基在皱纹Diutina rugosa产生脂肪酶的可持续生物过程发展中的潜在用途。首先，提出了一种包含葡萄糖，蛋白ept，酵母提取物，油酸和硫酸铵的合成培养基，其脂肪酶活性为143 U / L。然后，研究了由农业工业残留物制成的替代培养基，例如糖蜜，玉米浆（CSL）和橄榄磨废料（OMW）。进行了实验设计，发现仅CSL浓度对脂肪酶的产生具有积极作用。糖蜜（5 g / L），CSL（6 g / L），OMW（0.5％v）的混合物产生最高的脂肪酶活性（561 U / L）/ v），培养24小时时0.5 g / L硫酸铵和3 g / L蛋白L。脂肪酶的生产也在1-L生物反应器中进行，导致培养24小时时脂肪酶的活性略高。半纯化的酶的最佳温度和pH分别为40°C和7.0。最后，每单位生产的脂肪酶（UPC）的培养基成本受培养基成分的影响，特别是受所用诱导剂的影响。将农业工业残留物合并并以提高的浓度使用时，UPC最低。