Carbonic anhydrase (CA) was successfully used for carbon dioxide (CO₂) sequestration. In fact, sequestration of CO₂ into value-added products, calcium carbonate (CaCO₃), requires a stable and efficient CA that should tolerate high concentrations of CO₂ and Ca²⁺, high pH and high temperature of working process. Herein, for the first time, the present manuscript described the sequestration of CO₂ into CaCO₃ using a novel CA which was purified and characterized from liver of camel (Camelus dromedarius), an animal that survive extreme desert conditions. The enzyme was a monomer with lower molecular weight (25 kDa), contained Fe as a physiologically-relevant cofactor instead of Zn and showed higher optimum pH (pH 9.0) and temperature (45°C). In addition, the enzyme was active and stable at strongly alkaline pH (pH 9.0) and higher temperature (60°C). IC₅₀ values revealed that camel liver CA was inhibited by metal ions in the following order: Cu²⁺ > Zn²⁺ > Fe³⁺ > Cr³⁺ > Ni²⁺ > Cd²⁺ > Co²⁺ > Al³⁺ > Mg²⁺ > Ca²⁺, the enzyme needed high concentrations of Ca²⁺ to reach 50% inhibition. Camel liver CA was effective in accelerating CaCO₃ formation in presence of 1, 2, 5, 10 and 20% Ca²⁺. The formed CaCO₃ was characterized by SEM, XRD and FTIR. Interestingly, camel liver CA showed high CO₂ sequestration capacity (966.67 mg CaCO₃/mg enzyme) in presence of high concentration of Ca²⁺ (up to 20%). Camel CA represents promising candidate for harsh industrial applications.

碳酸酐酶（CA）已成功用于二氧化碳（CO ₂）隔离。实际上，将CO ₂螯合到增值产品碳酸钙（CaCO ₃）中需要稳定，有效的CA，该CA应该能够承受高浓度的CO ₂和Ca ²⁺，高pH值和高温的工作过程。在本文中，本手稿首次描述了使用从骆驼肝脏（Camelus dromedarius）纯化并鉴定的新型CA将CO ₂螯合成CaCO _3的方法。），可以在极端沙漠条件下生存的动物。该酶是一种具有较低分子量（25 kDa）的单体，含有Fe作为生理相关的辅因子，而不是Zn，并显示出较高的最佳pH（pH 9.0）和温度（45°C）。另外，该酶在强碱性pH（pH 9.0）和更高温度（60°C）下具有活性和稳定性。IC ₅₀值显示，骆驼肝CA被金属离子抑制的顺序如下：Cu ²⁺ > Zn ²⁺ > Fe ³⁺ > Cr ³⁺ > Ni ²⁺ > Cd ²⁺ > Co ²⁺ > Al ³⁺ >镁²⁺ >钙²⁺，该酶需要高浓度的Ca ²⁺才能达到50％的抑制率。骆驼肝CA在1、2、5、10和20％Ca ^2+的存在下可有效促进CaCO _3的形成。形成的CaCO ₃用SEM，XRD和FTIR表征。有趣的是，在高浓度的Ca ²⁺（高达20％）的情况下，骆驼肝CA表现出高的CO ₂隔离能力（966.67 mg CaCO ₃ / mg酶）。Camel CA代表了苛刻的工业应用的有希望的候选者。