Exploring Diffusion and Cellular Uptake: Charged Gold Nanoparticles in an in Vitro Breast Cancer Model,ACS Applied Bio Materials

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Exploring Diffusion and Cellular Uptake: Charged Gold Nanoparticles in an in Vitro Breast Cancer Model
ACS Applied Bio Materials ( IF 4.6 ) Pub Date : 2020-09-13 , DOI: 10.1021/acsabm.0c00872
Karolina P Valente _{1,

2} , Afzal Suleman ₁ , Alexandre G Brolo _{2,

3}

Affiliation

Gold nanoparticles have emerged as a prominent tool in nanomedicine, particularly for applications in cancer diagnostic and treatment. One of the challenges for the successful implementation of gold nanoparticles in cancer therapy is their delivery to the specific cancer area within the tumor microenvironment. The presence of cancer enables a poorly organized vascularization system, increasing the pressure with the microenvironment, limiting the uptake of particles. The physicochemical properties of the gold nanoparticles (size, shape, and surface charge) also have a significant effect on diffusion to the tumor site and cellular uptake. In this work, we analyzed the transport of 10 nm gold nanoparticles with different surface charges (neutral, negative, and positive) through a hydrogel composite. Three-dimensional in vitro models composed of breast cancer cells loaded in the hydrogel composite were used for the qualitative and quantitative evaluation of cellular uptake of the gold nanoparticles. Surprisingly, an inverse correlation between the diffusion coefficients of the nanoparticles and cellular uptake was demonstrated. Positively charged gold nanoparticles displayed high cellular uptake, although their diffusion coefficient indicated slow transport through the hydrogel matrix. Neutral particles, on the other hand, displayed fast diffusion but the lowest cellular uptake. The results obtained indicate that nanoparticle diffusion and cellular uptake should be studied together in realistic in vitro models for a true evaluation of transport in tumor microenvironments.

中文翻译：

探索扩散和细胞摄取：体外乳腺癌模型中的带电金纳米粒子

金纳米粒子已成为纳米医学中的重要工具，特别是在癌症诊断和治疗中的应用。在癌症治疗中成功实施金纳米粒子的挑战之一是将它们递送到肿瘤微环境中的特定癌症区域。癌症的存在使血管系统组织不良，增加了微环境的压力，限制了颗粒的吸收。金纳米粒子的物理化学性质（大小、形状和表面电荷）也对扩散到肿瘤部位和细胞摄取有显着影响。在这项工作中，我们分析了具有不同表面电荷（中性、负和正）的 10 nm 金纳米粒子通过水凝胶复合材料的传输。由负载在水凝胶复合材料中的乳腺癌细胞组成的三维体外模型用于定性和定量评估金纳米粒子的细胞摄取。令人惊讶的是，证明了纳米颗粒的扩散系数与细胞摄取之间的反相关。带正电荷的金纳米粒子显示出高细胞吸收，尽管它们的扩散系数表明通过水凝胶基质的缓慢传输。另一方面，中性粒子显示出快速扩散但细胞吸收最低。获得的结果表明，应在现实的体外模型中一起研究纳米颗粒扩散和细胞摄取，以真正评估肿瘤微环境中的转运。

更新日期：2020-10-21

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