Sensitive identification of prostate-specific antigen by iron oxide nanoparticle antibody conjugates on the gap-finger electrode surface,Biotechnology and Applied Biochemistry

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Sensitive identification of prostate-specific antigen by iron oxide nanoparticle antibody conjugates on the gap-finger electrode surface
Biotechnology and Applied Biochemistry ( IF 3.2 ) Pub Date : 2020-08-21 , DOI: 10.1002/bab.2012
Wei Zhang ₁ , Kai Li ₁ , Jingyang Guo ₁ , Tao Ma ₁ , Dongqing Wang ₁ , Sumei Shi ₁ , Subash C B Gopinath _{2,

3} , Deqiang Gu ₁

Affiliation

Researches have proved that increasing level of prostate-specific antigen (PSA) is an indicator for the progression of prostate cancer. The present study was focused to determine the PSA level by using anti-PSA antibody conjugated iron oxide nanoparticles, as the probe immobilized on the gap-fingered electrode sensing surface. The detection limit and sensitivity were found at the level of 1.9 pg/mL on the linear regression curve (y = 1.6939x − 0.5671; R² = 0.9878). A dose-dependent liner range was found from 1.9 until 60 pg/mL. Further, PSA was spiked in human serum and did not affect the interaction of PSA and its antibody. This method of detection quantifies the level of PSA, which helps to diagnose prostate cancer at its earlier stage.

中文翻译：

通过间隙指电极表面的氧化铁纳米颗粒抗体偶联物灵敏识别前列腺特异性抗原

研究证明，前列腺特异性抗原（PSA）水平的升高是前列腺癌进展的一个指标。本研究的重点是通过使用抗 PSA 抗体偶联氧化铁纳米颗粒来确定 PSA 水平，因为探针固定在间隙指电极传感表面。（检测限和灵敏度在1.9皮克/毫升的线性回归曲线的水平发现Ŷ = 1.6939 X - ; 0.5671 - [R 2 = 0.9878）。发现从 1.9 到 60 pg/mL 的剂量依赖性衬里范围。此外，PSA 被添加到人血清中并且不影响 PSA 与其抗体的相互作用。这种检测方法量化了 PSA 的水平，这有助于在早期诊断前列腺癌。

更新日期：2020-08-21

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