Background: This is the fastest work in obtaining the metabolic profiles of Pseudomonas aeruginosa in order to combat the infection diseases which leads to high morbidity and mortality rates. Pseudomonas aeruginosa is a high versatility of gram-negative bacteria that can undergo aerobic and anaerobic respiration. Capabilities in deploying different carbon sources, energy metabolism and regulatory system, ensure the survival of this microorganism in the diverse environment condition. Determination of differences in carbon sources utilization among biofilm and non-biofilm of Pseudomonas aeruginosa provides a platform in understanding the metabolic activity of the microorganism.

Methods: The study was carried out from September 2017 to February 2019. Four archive isolates forming strong and intermediate biofilm and non-biofilms producer were subcultured from archive isolates. ATCC 27853 P. aeruginosa was used as a negative control or non-biofilm producing microorganism. Biofilm formation was confirmed by Crystal Violet Assay (CVA) and Congo Red Agar (CRA). Metabolic profiles of the biofilm and non-biofilms isolates were determined by phenotype microarrays (Biolog Omnilog).

Results and Discussion: In this study, Pseudomonas aeruginosa biofilm isolates utilized uridine, L-threonine and L-serine while non-biofilm utilized adenosine, inosine, monomethyl, sorbic acid and succinamic acid.

Conclusion: The outcome of this result will be used for future studies to improve detection or inhibit the growth of P. aeruginosa biofilm and non-biofilm respectively.

背景：这是获得铜绿假单胞菌代谢谱以对抗导致高发病率和死亡率的感染疾病的最快工作。铜绿假单胞菌是革兰氏阴性菌的高通用性，可以进行有氧和无氧呼吸。部署不同碳源、能量代谢和调节系统的能力，确保了这种微生物在不同环境条件下的生存。确定铜绿假单胞菌的生物膜和非生物膜之间碳源利用的差异为了解微生物的代谢活动提供了一个平台。

方法：本研究于 2017 年 9 月至 2019 年 2 月进行，从档案分离株中传代培养形成强和中等生物膜和非生物膜生产者的 4 个档案分离株。ATCC 27853 铜绿假单胞菌用作阴性对照或非生物膜产生微生物。生物膜形成由结晶紫测定 ​​(CVA) 和刚果红琼脂 (CRA) 证实。生物膜和非生物膜分离物的代谢谱由表型微阵列（Biolog Omnilog）确定。

结果与讨论：在本研究中，铜绿假单胞菌生物膜分离物利用尿苷、L-苏氨酸和 L-丝氨酸，而非生物膜分离物利用腺苷、肌苷、单甲基、山梨酸和琥珀酸。

结论：该结果的结果将用于未来的研究，以分别提高检测或抑制铜绿假单胞菌生物膜和非生物膜的生长。