Probiotic bacteria are known to treat and prevent diseases and hence promote physical and mental wellness due to their significant brain-gut relationship. The main challenge involved in probiotic commercialization is the bio processing limitation to produce high cell mass, especially with the cultivation of lactic acid bacteria which produces lactic acid as a by product. Synthesis of lactic acid by lactic acid bacteria inhibits bacterial growth, and in turn disrupts high cell mass production. Current work presents the findings for Lactobacillus casei medium optimization by response surface methodology in shake flask level. A simple medium using 4 components: lactose, soybean meal, yeast extract and magnesium sulphate has been identified to produce high cell mass than generic mediaused for probiotic cultivation, such as the MRS medium. Secondly, response surface methodology using Box-Behken Design was employed as an optimization strategy. After optimization process, the production of Lactobacillus casei biomass increased by about 164.6% recording 6.51g.L^-1 compared to cell biomass obtained using initial un-optimized medium (2.46g.L^-1).

中文翻译：

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干酪乳杆菌高细胞生物量生产的统计优化方法

益生菌因其重要的肠胃关系而已知可治疗和预防疾病，因此可促进身心健康。益生菌商业化所涉及的主要挑战是产生高细胞量的生物加工限制，尤其是培养乳酸菌时会产生乳酸作为副产物。乳酸菌合成乳酸会抑制细菌的生长，进而破坏大量细胞的生产。当前的工作提出了干酪乳杆菌的发现摇瓶水平通过响应面方法进行培养基优化。已经确定了一种使用4种成分的简单培养基：乳糖，豆粕，酵母提取物和硫酸镁，与用于益生菌培养的通用培养基（例如MRS培养基）相比，产生的细胞量更高。其次，采用Box-Behken设计的响应面方法作为优化策略。经过优化处理后，与使用初始未优化培养基（2.46gL ^-1）获得的细胞生物量相比，干酪乳杆菌生物量的产量增加了约164.6％，记录了6.51gL ^-1。