Orthogonal Blue and Red Light Controlled Cell-Cell Adhesions Enable Sorting-out in Multicellular Structures.,ACS Synthetic Biology

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Orthogonal Blue and Red Light Controlled Cell-Cell Adhesions Enable Sorting-out in Multicellular Structures.
ACS Synthetic Biology ( IF 3.7 ) Pub Date : 2020-07-01 , DOI: 10.1021/acssynbio.0c00150
Samaneh Rasoulinejad ₁ , Marc Mueller ₁ , Brice Nzigou Mombo ₂ , Seraphine V Wegner _{1,

2}

Affiliation

The self-assembly of different cell types into multicellular structures and their organization into spatiotemporally controlled patterns are both challenging and extremely powerful to understand how cells function within tissues and for bottom-up tissue engineering. Here, we not only independently control the self-assembly of two cell types into multicellular architectures with blue and red light, but also achieve their self-sorting into distinct assemblies. This required developing two cell types that form selective and homophilic cell–cell interactions either under blue or red light using photoswitchable proteins as artificial adhesion molecules. The interactions were individually triggerable with different colors of light, reversible in the dark, and provide noninvasive and temporal control over the cell–cell adhesions. In mixtures of the two cells, each cell type self-assembled independently upon orthogonal photoactivation, and cells sorted out into separate assemblies based on specific self-recognition. These self-sorted multicellular architectures provide us with a powerful tool for producing tissue-like structures from multiple cell types and investigate principles that govern them.

中文翻译：

正交的蓝光和红光控制的细胞间粘附使得能够在多细胞结构中进行分类。

要了解细胞在组织内的功能以及自下而上的组织工程，将不同类型的细胞自组装成多细胞结构并将它们组织成时空受控模式既具有挑战性，又非常强大。在这里，我们不仅用蓝光和红光将两种细胞类型的自组装独立控制为多细胞结构，而且还实现了将它们自分类为不同的组装。这就需要开发两种细胞类型，在蓝色或红色光下使用光可开关蛋白作为人工粘附分子，形成选择性的和同质的细胞间相互作用。相互作用可以分别用不同颜色的光触发，在黑暗中可逆，并提供对细胞-细胞粘附的非侵入性和暂时性控制。在这两种细胞的混合物中，每种细胞类型均在正交光激活后独立自组装，并且根据特定的自我识别将细胞分类为单独的组件。这些自分类的多细胞体系结构为我们提供了一种强大的工具，可以从多种细胞类型中产生组织样结构，并研究控制它们的原理。

更新日期：2020-08-21

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