Xylose-inducible modules simultaneously expressing xylose utilization and naringenin biosynthesis pathways were developed in Yarrowia lipolytica to produce naringenin from a mixture of glucose and xylose. The naringenin synthetic pathway was constructed using a constitutive expression to yield 239.1 ± 5.1 mg/L naringenin. Furthermore, the introduction of an inducible pathway realized the dual function of xylose as a substrate and synthetic inducer, which coupled the xylose utilization with naringenin biosynthesis and increased production. Interestingly, the simultaneous enhancement of xylose reductase and xylose transporter expression along with that of xylitol dehydrogenase and xylulokinase can further improve the xylose utilization ability of Y. lipolytica. As expected, xylose-inducible synthesis of naringenin could achieved a titer of 715.3 ± 12.8 mg/L through the shake-flask cultivation level. Therefore, xylose-induced activation of both the xylose utilization and product biosynthesis pathway is considered to be an effective strategy for the biosynthesis of xylose-derived chemicals in yeast.

在解脂耶氏酵母中开发了同时表达木糖利用和柚皮素生物合成途径的木糖诱导模块，以由葡萄糖和木糖的混合物产生柚皮素。使用组成型表达构建柚皮苷合成途径，以产生239.1±5.1 mg / L柚皮苷。此外，诱导途径的引入实现了木糖作为底物和合成诱导剂的双重功能，这将木糖利用与柚皮素的生物合成结合起来并提高了产量。有趣的是，木糖还原酶和木糖转运蛋白表达的同时增强以及木糖醇脱氢酶和木酮糖激酶的增强可以进一步提高解脂耶氏酵母的木糖利用能力。。如预期的那样，通过摇瓶培养水平，木糖诱导的柚皮苷合成可以达到715.3±12.8 mg / L的效价。因此，木糖诱导的木糖利用和产物生物合成途径两者的活化被认为是酵母中木糖衍生的化学物质的生物合成的有效策略。