Supercritical CO₂ extraction experiments were conducted to investigate the effects of pretreatment and process parameters on neutral lipids, chlorophylls and carotenoids recovery on two species of Nannochloropsis. For Nannochloropsis maritima, a factorial experimental design was performed (P: [100–300] bar, T: [313–333] K). The highest extraction yields were obtained at the highest pressures and temperatures. Two drying modes, ring drying and air flow drying, were compared. Although total extraction yield and extraction kinetics were observed to be greater using air flow dried microalgae, extracts from this drying method resulted in partial degradation of glycerides in free fatty acids. Ring dried extracts maintained the same neutral lipid composition as the initial biomass. Based on these results, ring dried Nannochloropsis salina was extracted using supercritical CO₂ at 333 K and both 300–400 bar. Extraction curves were modelled using the Sovová’s mathematical model.

进行超临界CO ₂萃取实验，以研究预处理和工艺参数对两种拟南芥中性脂质，叶绿素和类胡萝卜素回收率的影响。对于Nannochloropsis maritima，进行了阶乘实验设计（P：[100-300] bar，T：[313-333] K）。在最高的压力和温度下获得最高的提取率。比较了环形干燥和气流干燥两种干燥模式。尽管使用气流干燥的微藻可观察到总的提取得率和提取动力学更高，但这种干燥方法的提取物却导致了游离脂肪酸中甘油酯的部分降解。环形干燥的提取物保持与初始生物质相同的中性脂质组成。基于这些结果，使用超临界CO ₂在333 K和300-400 bar的压力下提取了环状干燥的Nannochloropsis salina。提取曲线使用Sovová的数学模型建模。