To characterize their virome, double stranded RNAs extracted from scrapings of two Iranian grapevine varieties held in the Vassal-Montpellier Grapevine Biological Resources Center were analysed by high-throughput sequencing. In addition to several well-known grapevine viruses, divergent isolates of the newly described grapevine Kizil Sapak virus were identified in both accessions. Four complete genome sequences were determined, as well as two additional partial sequences (1,580 and 3,849 nucleotides long). These genomic sequences highlight the molecular diversity of this poorly known virus. In view of the absence of amplification of the GKSV isolates characterized here using the published primer pair, novel degenerate, polyvalent primers were designed, providing a more robust diagnosis.

为了表征其病毒性，通过高通量测序分析了从Vassal-Montpellier葡萄生物资源中心的两个伊朗葡萄品种的碎片中提取的双链RNA。除了几种众所周知的葡萄病毒之外，在两个种中都鉴定出了新描述的葡萄科齐尔Sapak病毒的不同分离株。确定了四个完整的基因组序列，以及两个附加的部分序列（长1,580和3,849个核苷酸）。这些基因组序列突出了这种鲜为人知的病毒的分子多样性。考虑到这里没有使用公开的引物对来扩增GKSV分离株，因此设计了新颖的简并多价引物，以提供更可靠的诊断。