Abstract The present work aimed to characterize new aqueous two-phase system (ATPS) formed by Triton X-100 + Li2SO4 + H2O, polyvinylpyrrolidone Mw = 10,000 g mol−1 (PVP10k) + Li2SO4 + H2O, and Triton X-100 + PVP10k + H2O, evaluating the effect of temperature and the nature of ATPS-forming components on the biphasic region. ATPSs formed by Li2SO4 showed greater efficiency in phase segregation compared to ATPSs formed by polymer/surfactant, with the biphasic area of ATPSs formed by Triton X-100 greater than that formed by PVP10k. For all ATPSs studied, temperature had a small effect on the biphasic region. The data were fitted with good correlation using the Merchuk equation for the binodal curves and Othmer–Tobias and Bancroft equations for the tie-lines. The partitioning studies show that there is an uneven distribution of Reactive Yellow HE-4R dye between the phases for all ATPSs and, with a tie-line length increase, there is an increase in the tendency of the dye to partition to the phase in which it interacts most strongly. For ATPSs formed by Li2SO4, the dye is preferentially transferred to the macromolecule-rich phase (top phase), whereas for the system formed by polymer/surfactant, the dye is partitioned to the PVP10k enriched phase (bottom phase).

中文翻译：

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由不同相形成组分形成的水性两相系统：平衡图和染料分配研究

摘要 目前的工作旨在表征由 Triton X-100 + Li2SO4 + H2O、聚乙烯吡咯烷酮 Mw = 10,000 g mol−1 (PVP10k) + Li2SO4 + H2O 和 Triton X-100 + PVP10k 形成的新型含水两相系统 (ATPS) + H2O，评估温度的影响和形成 ATPS 的成分对双相区域的性质。与由聚合物/表面活性剂形成的 ATPS 相比，由 Li2SO4 形成的 ATPS 在相分离方面表现出更高的效率，Triton X-100 形成的 ATPS 的双相面积大于 PVP10k 形成的双相面积。对于所有研究的 ATPS，温度对双相区域的影响很小。使用双节点曲线的 Merchuk 方程和连接线的 Othmer-Tobias 和 Bancroft 方程拟合数据具有良好的相关性。分配研究表明，活性黄 HE-4R 染料在所有 ATPS 的相之间分布不均匀，并且随着连接线长度的增加，染料分配到其中的相的趋势增加它相互作用最强烈。对于由 Li2SO4 形成的 ATPS，染料优先转移到富含高分子的相（上相），而对于由聚合物/表面活性剂形成的体系，染料被分配到富含 PVP10k 的相（下相）。