Photodynamic therapy (PDT) is a noninvasive therapy based on the photodynamic effect. In this study, we sought to determine intracellular uptake and in vivo photodynamic therapy potential of Zn phthalocyanine-loaded mesoporous silica nanoparticles (MSNP5) against pancreatic cancer cells. MSNP5 were labeled with ¹³¹I; the radiolabeling efficiency was found to 95.5 ± 1.2% in pH 9 and 60 min reaction time. Besides, the highest intracellular uptake yields of ¹³¹I-MSNP5 nanoparticles in MIA PaCa-2, AsPC-1, and PANC-1 cells were determined as 43.9 ± 3.8%, 41.8 ± 0.2%, and 37.9 ± 1.3%, respectively, at 24 h incubation time. In vivo PDT studies were performed with subcutaneous xenograft cancer model nude mice with AsPC-1 pancreatic cancer cells. For photodynamic therapy, 685 nm red laser light 100 J/cm² light dose using and 5–20 μM ZnPc containing MSNP5 concentrations were applied. Histopathological studies revealed that the ratio of necrosis in tumor tissue was higher in the treatment group than the control groups.

中文翻译：

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含有锌酞菁的目标介孔二氧化硅纳米粒子的放射性标记，体外细胞摄取和体内光动力治疗潜力。

光动力疗法（PDT）是一种基于光动力效应的无创疗法。在这项研究中，我们试图确定负载锌酞菁的介孔二氧化硅纳米颗粒（MSNP5）对胰腺癌细胞的细胞内吸收和体内光动力疗法的潜力。MSNP5标记为¹³¹ I；在pH 9和60分钟的反应时间中，放射性标记效率达到95.5±1.2％。此外，在MIA PaCa-2，AsPC-1和PANC-1细胞中，¹³¹ I-MSNP5纳米颗粒的最高细胞内摄取率分别确定为43.9±3.8％，41.8±0.2％和37.9±1.3％。孵育时间为24小时。体内用具有AsPC-1胰腺癌细胞的皮下异种移植癌模型裸鼠进行PDT研究。对于光动力疗法，使用685 nm的100 J / cm ²光剂量的红色激光和5-20μM含MSNP5浓度的ZnPc。组织病理学研究显示，治疗组中肿瘤组织中坏死的比例高于对照组。