ZnO nanoparticles are widely used in biological, chemical, and medical fields, but their toxicity impedes their wide application. In this study, pristine ZnO NPs (~ 7 nm; ~ 18 nm; ~ 49 nm) and lipid-coated ZnO NPs (~ 13 nm; ~ 22 nm; ~ 52 nm) with different morphologies were prepared by chemical method and characterized by TEM, XRD, HRTEM, FTIR, and DLS. Our results showed that the lipid-coated ZnO NPs (~ 13 nm; ~ 22 nm; ~ 52 nm) groups improved the colloidal stability, prevented the aggregation and dissolution of nanocrystal particles in the solution, inhibited the dissolution of ZnO NPs into Zn2+ cations, and reduced cytotoxicity more efficiently than the pristine ZnO NPs (~ 7 nm; ~ 18 nm; ~ 49 nm). Compared to the lipid-coated ZnO NPs, pristine ZnO NPs (~ 7 nm; ~ 18 nm; ~ 49 nm) could dose-dependently destroy the cells at low concentrations. At the same concentration, ZnO NPs (~ 7 nm) exhibited the highest cytotoxicity. These results could provide a basis for the toxicological study of the nanoparticles and direct future investigations for preventing strong aggregation, reducing the toxic effects of lipid-bilayer and promoting the uptake of nanoparticles by HeLa cells efficiently.

中文翻译：

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脂质包裹的ZnO纳米粒子在癌细胞中的合成，表征和细胞毒性研究。

ZnO纳米粒子广泛用于生物，化学和医学领域，但其毒性阻碍了其广泛应用。在这项研究中，通过化学方法制备了具有不同形态的原始ZnO NPs（〜7 nm;〜18 nm;〜49 nm）和脂质包覆的ZnO NPs（〜13 nm;〜22 nm;〜52 nm），并通过以下方法进行了表征TEM，XRD，HRTEM，FTIR和DLS。我们的结果表明，脂质包覆的ZnO NPs（〜13 nm;〜22 nm;〜52 nm）基团改善了胶体稳定性，防止了溶液中纳米晶体颗粒的聚集和溶解，抑制了ZnO NPs溶解为Zn2 +阳离子。 ，并且比原始的ZnO NPs（〜7 nm;〜18 nm;〜49 nm）更有效地降低了细胞毒性。与脂质包覆的ZnO NPs相比，原始的ZnO NPs（〜7 nm；〜18 nm；〜49 nm）可以剂量依赖性地破坏低浓度的细胞。在相同浓度下，ZnO NP（〜7 nm）表现出最高的细胞毒性。这些结果可为纳米颗粒的毒理学研究提供基础，并为防止强聚集，降低脂质双层的毒性作用以及有效促进HeLa细胞对纳米颗粒的吸收提供直接指导。