A new exceptionally-preserved Cambrian biota, with fossils belonging to multiple phyla, has recently been found from the middle part of the Longha Formation, near Fulu Village, southeastern Yunnan, South China, and is named the Fulu biota. Groups recovered so far include polymerid trilobites, agnostoids, large bivalved arthropods, bradoriid arthropods, echinoderms, brachiopods, priapulids (worms), hyoliths, macroscopic algae, and trace fossils. The arthropods dominate the biota and are highly diverse with echinoderms second in diversity. The associated agnostoids provide a precise late Guzhangian Age (late Miaolingian Epoch) for the biota, indicating that the biota occurs stratigraphically about halfway between the occurrences of the Kaili and the Guole biotas of South China, from both of which the new Fulu biota differs significantly in faunal components. Guzhangian biotas preserving poorly mineralized tissues and complete echinoderms have been previously unknown in Gondwana until now; thus, the discovery of Fulu biota provides a unique window into life at this critical time interval.