Mounting evidence showing that local nitric oxide (NO) delivery may significantly improve the wound healing process has stimulated the development of wound dressings capable of releasing NO topically. Herein, we describe the preparation of a self‐expandable NO‐releasing hydrolyzed collagen sponge (CS), charged with the endogenously found NO donor, S‐nitrosoglutathione (GSNO). We show that cold pressed and GSNO‐charged CS (CS/GSNO) undergo self‐expansion to its original 3D shape upon water absorption to a swelling degree of 2,300 wt%, triggering the release of free NO. Topical application of compressed CS/GSNO on wounds in an animal model showed that exudate absorption by CS/GSNO leads to the release of higher NO doses during the inflammatory phase and progressively lower NO doses at later stages of the healing process. Moreover, treated animals showed significant increase in the mRNA expression levels of monocyte chemoattractant protein‐1 (MCP‐1), murine macrophage marker (F4/80), transforming growth factor beta (TGF‐β), stromal cell‐derived factor 1 (SDF‐1), insulin‐like growth factor‐1 (IGF‐1), nitric oxide synthase(iNOS), and matrix metalloproteinase(MMP‐9). Cluster differentiation 31 (CD31), vascular endothelial growth factor (VEGF), and F4/80 were measured on Days 7 and 12 by immunohistochemistry in the cicatricial tissue. These results indicate that the topical delivery of NO enhances the migration and infiltration of leucocytes, macrophages, and keratinocytes to the wounded tissue, as well as the neovascularization and collagen deposition, which are correlated with an accelerated wound closure. Thus, self‐expandable CS/GSNO may represent a novel biocompatible and active wound dress for the topical delivery of NO on wounds.

中文翻译：

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释放一氧化氮的自膨胀胶原海绵的伤口愈合作用。

越来越多的证据表明，局部一氧化氮 (NO) 递送可以显着改善伤口愈合过程，这刺激了能够局部释放 NO 的伤口敷料的发展。在此，我们描述了一种自膨胀的 NO 释放水解胶原海绵（CS）的制备，该海绵带有内源性发现的 NO 供体 S-亚硝基谷胱甘肽（GSNO）。研究表明，冷压且带 GSNO 的 CS（CS/GSNO）在吸水至 2,300 wt% 的溶胀度后会自膨胀至其原始 3D 形状，从而引发游离 NO 的释放。在动物模型的伤口上局部应用压缩的 CS/GSNO 表明，CS/GSNO 的渗出液吸收导致炎症阶段释放更高的 NO 剂量，并在愈合过程的后期逐渐降低 NO 剂量。而且，经治疗的动物显示单核细胞趋化蛋白-1 (MCP-1)、鼠巨噬细胞标记物 (F4/80)、转化生长因子β (TGF-β)、基质细胞衍生因子 1 (SDF- 1)、胰岛素样生长因子-1 (IGF-1)、一氧化氮合酶 (iNOS) 和基质金属蛋白酶 (MMP-9)。在第 7 天和第 12 天通过瘢痕组织中的免疫组织化学测量簇分化 31 (CD31)、血管内皮生长因子 (VEGF) 和 F4/80。这些结果表明，NO 的局部递送增强了白细胞、巨噬细胞和角质形成细胞向受伤组织的迁移和浸润，以及与加速伤口闭合相关的新血管形成和胶原沉积。因此，