Biogas production and microbial community structure were analyzed as an effect of biochar addition to a fermentation sludge containing sugar beet pulp. Positive effects of the treatment including an increase in process efficiency and better biogas quality were noted. The effect of biochar on AD (anaerobic digestion process) microbial communities was investigated after total DNA extraction from biochar-amended fermentation mixtures by PCR amplification of bacterial 16S rRNA gene fragments and Illumina amplicon sequencing. A combination of microbiological and physico-chemical analyses was used to study the mechanism by which biochar influences the process of anaerobic digestion of sugar beep pulp. It was found that the main reason of the changes in biogas production was the reshaping of the microbial communities, in particular enrichment of Bacteroidales and Clostridiales. It was proposed that biochar, in addition to being a conductor for mediating interspecies electron transfer, serves also as a habitat for hydrolytic bacteria. It was elucidated that the main driving force for the preferential colonization of biochar surfaces is its hydrophobicity. The presented research indicates the high potential of biochar to stimulate the methane fermentation process.

中文翻译：

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添加生物炭可增强甜菜废物（纸浆）甲烷化中微生物间的合作。

分析了沼气的产生和微生物群落结构，作为生物炭添加到含糖用甜菜浆的发酵污泥中的影响。注意到该处理的积极效果，包括过程效率的提高和更好的沼气质量。通过细菌16S rRNA基因片段的PCR扩增和Illumina扩增子测序从生物炭改良的发酵混合物中总DNA提取后，研究了生物炭对AD（厌氧消化过程）微生物群落的影响。结合微生物学和理化分析来研究生物炭影响甜菜浆厌氧消化过程的机理。发现沼气生产变化的主要原因是微生物群落的重塑，尤其是细菌和梭菌的富集。有人提出，生物炭除了作为介导种间电子转移的导体外，还可以作为水解细菌的栖息地。阐明了生物炭表面优先定居的主要驱动力是其疏水性。提出的研究表明生物炭具有刺激甲烷发酵过程的巨大潜力。