In Vivo Flow Cytometric Evaluation of Circulating Metastatic Pancreatic Tumor Cells after High-Intensity Focused Ultrasound Therapy.,Cytometry Part A

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In Vivo Flow Cytometric Evaluation of Circulating Metastatic Pancreatic Tumor Cells after High-Intensity Focused Ultrasound Therapy.
Cytometry Part A ( IF 2.5 ) Pub Date : 2020-04-19 , DOI: 10.1002/cyto.a.24014
Qian Yu _{1,

2} , Yijing Yao _{1,

2} , Xi Zhu ₃ , Yihui Gao _{1,

2} , Yini Chen _{1,

2} , Rui Wang _{1,

2} , Pingping Xu _{1,

2} , Xunbin Wei _{3,

4} , Lixin Jiang _{1,

2}

Affiliation

We examined our hypothesis that high‐intensity focused ultrasound (HIFU) treatment of pancreatic ductal adenocarcinoma (PDAC) in nude mice models may lead to an increased occurrence of hematogenous metastasis. The human PDAC cell line BxPC‐3 transfected with mCherry was implanted into nude mice to establish orthotopic and subcutaneous xenograft (OX and SX) tumor models. Mice were exposed to HIFU when tumor sizes reached approximately 200–300 mm³. The OX and SX tumor models were monitored continuously for tumor growth characteristics and hematogenous metastasis using in vivo flow cytometric (IVFC) detection of circulating tumor cells (CTCs) from the pancreas. We chose an appropriate mouse model to further examine whether or not HIFU increases the potential risk of hematogenous metastasis, using IVFC detection. Our results showed that the CTC number was greater in the OX model than in the SX model. The CTC number in the OX model increased gradually over time, whereas the CTC number in the SX model remained low. Therefore, the OX model was better for studying tumor metastasis by IVFC detection. We found significantly decreased CTC numbers and tumor volume after HIFU ablation. Our results showed the applicability of the PDAC OX tumor model for studying the occurrence of tumor metastasis due to the generation of CTCs. HIFU ablation substantially restricted PDAC hematogenous metastasis and provided effective tumor control locally. © 2020 The Authors. Cytometry Part A published by Wiley Periodicals Inc., on behalf of International Society for Advancement of Cytometry.

中文翻译：

高强度聚焦超声治疗后循环转移性胰腺肿瘤细胞的体内流式细胞术评估。

我们检验了我们的假设，即在裸鼠模型中高强度聚焦超声 (HIFU) 治疗胰腺导管腺癌 (PDAC) 可能导致血行转移的发生率增加。将转染 mCherry 的人 PDAC 细胞系 BxPC-3 植入裸鼠体内，建立原位和皮下异种移植（OX 和 SX）肿瘤模型。当肿瘤大小达到约 200-300 mm ³时，小鼠会暴露于 HIFU. 使用来自胰腺的循环肿瘤细胞 (CTC) 的体内流式细胞术 (IVFC) 检测连续监测 OX 和 SX 肿瘤模型的肿瘤生长特征和血行转移。我们选择了一个合适的小鼠模型，使用 IVFC 检测进一步检查 HIFU 是否会增加血行转移的潜在风险。我们的结果表明，OX 模型中的 CTC 数量大于 SX 模型。OX 模型中的 CTC 数量随着时间的推移逐渐增加，而 SX 模型中的 CTC 数量仍然很低。因此，OX模型更适合通过IVFC检测来研究肿瘤转移。我们发现 HIFU 消融后 CTC 数量和肿瘤体积显着降低。我们的结果表明 PDAC OX 肿瘤模型适用于研究由于 CTC 的产生而发生的肿瘤转移。HIFU 消融大大限制了 PDAC 的血行转移，并在局部提供了有效的肿瘤控制。© 2020 作者。Cytometry Part A由 Wiley Periodicals Inc. 代表 International Society for Advancement of Cytometry 出版。

更新日期：2020-04-19

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