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The sol-gel-sol transformation behavior of egg white proteins induced by alkali.
International Journal of Biological Macromolecules ( IF 7.7 ) Pub Date : 2020-03-28 , DOI: 10.1016/j.ijbiomac.2020.03.209 Xuejing Gao 1 , Yao Yao 1 , Na Wu 1 , Mingsheng Xu 1 , Yan Zhao 2 , Yonggang Tu 1
International Journal of Biological Macromolecules ( IF 7.7 ) Pub Date : 2020-03-28 , DOI: 10.1016/j.ijbiomac.2020.03.209 Xuejing Gao 1 , Yao Yao 1 , Na Wu 1 , Mingsheng Xu 1 , Yan Zhao 2 , Yonggang Tu 1
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In the current study, we found an interesting phenomenon that fresh egg white (EW) undergo the sol-gel-sol transition with alkali treatment. The transformation behavior at different alkalinity (1.5%, 2.0%, and 2.5%) was investigated. As the gel formed, the hardness, lightness, surface hydrophobicity and the total number of identified peptides increased, and then, remarkable reduction when the gel collapsed. Rheological behavior indicated that the viscosity varied with shear rate. Fourier transform infrared spectroscopy (FTIR) showed that β-sheets gradually decreased as the α-helices increased during gel-sol transformation. The quantification of EW peptides analysis revealed that there was no dramatic correlation between the number of identified peptides and alkalinity. It was concluded that the sol-gel-sol transition was strongly dependent on alkali levels, moreover, high concentration promoted gel formation as well as liquefaction. The EW transformation behavior induced by alkali had a significant effect on protein aggregation and denaturation, and further changed physicochemical properties.
中文翻译：
碱诱导蛋清蛋白的溶胶-凝胶-溶胶转化行为。
在当前的研究中，我们发现了一个有趣的现象，即新鲜的蛋清（EW）经过碱处理后发生了溶胶-凝胶-溶胶转变。研究了在不同碱度（1.5％，2.0％和2.5％）下的转化行为。随着凝胶的形成，硬度，亮度，表面疏水性和鉴定出的肽总数增加，然后当凝胶塌陷时显着减少。流变行为表明粘度随剪切速率而变化。傅里叶变换红外光谱（FTIR）表明，在凝胶-溶胶转化过程中，随着α-螺旋的增加，β-折叠逐渐减少。EW肽分析的定量分析表明，鉴定出的肽数量与碱度之间无显着相关性。结论是，溶胶-凝胶-溶胶的转变强烈依赖于碱水平，此外，高浓度促进了凝胶的形成和液化。碱诱导的EW转化行为对蛋白质的聚集和变性具有重要影响，并进一步改变其理化性质。
更新日期：2020-03-28
中文翻译：
碱诱导蛋清蛋白的溶胶-凝胶-溶胶转化行为。
在当前的研究中，我们发现了一个有趣的现象，即新鲜的蛋清（EW）经过碱处理后发生了溶胶-凝胶-溶胶转变。研究了在不同碱度（1.5％，2.0％和2.5％）下的转化行为。随着凝胶的形成，硬度，亮度，表面疏水性和鉴定出的肽总数增加，然后当凝胶塌陷时显着减少。流变行为表明粘度随剪切速率而变化。傅里叶变换红外光谱（FTIR）表明，在凝胶-溶胶转化过程中，随着α-螺旋的增加，β-折叠逐渐减少。EW肽分析的定量分析表明，鉴定出的肽数量与碱度之间无显着相关性。结论是，溶胶-凝胶-溶胶的转变强烈依赖于碱水平，此外，高浓度促进了凝胶的形成和液化。碱诱导的EW转化行为对蛋白质的聚集和变性具有重要影响，并进一步改变其理化性质。
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