Rotational Diffusion of Membrane Proteins in Crowded Membranes,The Journal of Physical Chemistry B

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Rotational Diffusion of Membrane Proteins in Crowded Membranes
The Journal of Physical Chemistry B ( IF 2.8 ) Pub Date : 2020-04-01 , DOI: 10.1021/acs.jpcb.0c00884
Matti Javanainen _{1,

2} , O. H. Samuli Ollila ₃ , Hector Martinez-Seara ₁

Affiliation

Membrane proteins travel along cellular membranes and reorient themselves to form functional oligomers and protein–lipid complexes. Following the Saffman–Delbrück model, protein radius sets the rate of this diffusive motion. However, it is unclear how this model, derived for ideal and dilute membranes, performs under crowded conditions of cellular membranes. Here, we study the rotational motion of membrane proteins using molecular dynamics simulations of coarse-grained membranes and 2-dimensional Lennard-Jones fluids with varying levels of crowding. We find that the Saffman–Delbrück model captures the size-dependency of rotational diffusion under dilute conditions where protein−protein interactions are negligible, whereas stronger scaling laws arise under crowding. Together with our recent work on lateral diffusion, our results reshape the description of protein dynamics in native membrane environments: The translational and rotational motions of proteins with small transmembrane domains are rapid, whereas larger proteins or protein complexes display substantially slower dynamics.

中文翻译：

膜蛋白在拥挤膜中的旋转扩散

膜蛋白沿着细胞膜移动并重新定向以形成功能性低聚物和蛋白-脂质复合物。根据Saffman–Delbrück模型，蛋白质半径决定了这种扩散运动的速率。但是，尚不清楚这种理想膜和稀膜的模型在拥挤的细胞膜条件下如何发挥作用。在这里，我们使用分子筛模拟的粗粒度膜和二维拥挤水平的Lennard-Jones流体来研究膜蛋白的旋转运动。我们发现，Saffman–Delbrück模型在稀疏条件下捕获了旋转扩散的尺寸依赖性，在稀薄条件下，蛋白质与蛋白质之间的相互作用可忽略不计，而在拥挤情况下会出现更强的缩放定律。加上我们最近在横向扩散方面的工作，

更新日期：2020-04-01

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