ABSTRACT Collagen is the most abundant protein in humans and animals, comprising of one third of the total proteins that accounts for three quarters of the dry weight skin in humans. Collagen containing a range of proteins has been reported for tissue engineering applications, but, only a small number of studies related to chemical structure evaluation of collagen are found in the literature. Collagen can be obtained from both the natural and synthetic sources and offers a wide range of biomedical applications due to its excellent biocompatibility and low immunogenicity. Hence, it is important to identify chemical structural properties of collagen and Fourier transform infrared (FTIR) appears to be a technique of choice to study their chemical structure. This review aims to highlight the use of FTIR to study collagen-based biomaterials, using it for characterization of collagen extracted from various sources. Characterization of collagen-based materials used in wound healing, skin substitutes, derma fillers, and aging of skin, collagen containing drug delivery agents, collagen-based materials used in tissue engineering, bone regeneration, and osteogenic differentiation is discussed in detail. FTIR analysis of collagen-containing materials used for dental applications, cleft-palate, and in alveolar-ridge preservation has also been highlighted.

中文翻译：

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天然和合成胶原蛋白的 FTIR 分析

摘要 胶原蛋白是人类和动物中含量最丰富的蛋白质，占人体总蛋白质的三分之一，占人体皮肤干重的四分之三。已经报道了含有一系列蛋白质的胶原蛋白用于组织工程应用，但是，在文献中只发现了少数与胶原蛋白化学结构评估相关的研究。胶原蛋白可以从天然和合成来源获得，由于其优异的生物相容性和低免疫原性，可提供广泛的生物医学应用。因此，确定胶原蛋白的化学结构特性很重要，傅里叶变换红外 (FTIR) 似乎是研究其化学结构的首选技术。本综述旨在强调使用 FTIR 研究基于胶原的生物材料，使用它来表征从各种来源提取的胶原蛋白。详细讨论了用于伤口愈合、皮肤替代品、真皮填充剂和皮肤老化、含有胶原蛋白的药物递送剂、用于组织工程、骨再生和成骨分化的胶原基材料的表征。用于牙科应用、腭裂和牙槽嵴保存的含胶原材料的 FTIR 分析也得到了强调。和成骨分化进行了详细讨论。用于牙科应用、腭裂和牙槽嵴保存的含胶原材料的 FTIR 分析也得到了强调。和成骨分化进行了详细讨论。用于牙科应用、腭裂和牙槽嵴保存的含胶原材料的 FTIR 分析也得到了强调。