Automated on-line packed fiber solid phase extraction for determination of urinary catecholamines.,Journal of Chromatography B

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Automated on-line packed fiber solid phase extraction for determination of urinary catecholamines.
Journal of Chromatography B ( IF 2.8 ) Pub Date : 2020-01-18 , DOI: 10.1016/j.jchromb.2020.121983
LiQin Chen ₁ , Yi Tang ₁ , Bei Xu ₁ , Zhen Xu ₁ , Jun Shen ₁ , WanQi Zhang ₁

Affiliation

Despite the development of an off-line packed fiber solid phase extraction procedure (PFSPE) for urinary catecholamines, automation remains a challenge. Here, we propose an on-line PFSPE-HPLC procedure for automated sample processing and analysis of urinary catecholamines, with good recovery and precision, to avoid manual operation errors. The on-line PFSPE-HPLC procedure has been thoroughly optimized concerning the gradient, valve switch timing, the effects of complexing reagent and buffer solution, and the stability of the nanofibers. Validation of the developed on-line PFSPE-HPLC protocol in urine yielded satisfactory accuracies of 99.6-104.2%, precision below 7.0%, as well as a linear range from 1 ng/mL to 100 ng/mL with a correlation coefficient of 0.999. The developed protocol is herein presented as a potential technology for automated sample pretreatment for the determination of urinary catecholamines.

中文翻译：

自动在线填充纤维固相萃取法测定尿儿茶酚胺。

尽管开发了用于尿儿茶酚胺的离线填充纤维固相萃取程序（PFSPE），但自动化仍然是一个挑战。在这里，我们提出了一种在线PFSPE-HPLC程序，用于尿儿茶酚胺的自动样品处理和分析，具有良好的回收率和精密度，可避免人工操作错误。在线PFSPE-HPLC程序已针对梯度，阀切换时机，络合剂和缓冲液的影响以及纳米纤维的稳定性进行了全面优化。尿液中开发的在线PFSPE-HPLC方案的验证产生了令人满意的准确度，为99.6-104.2％，精度低于7.0％，线性范围为1 ng / mL至100 ng / mL，相关系数为0.999。

更新日期：2020-01-21

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