Since the first work by Laurencin and colleagues on the development of polymeric electrospinning for biomedical purposes, the use of electrospinning technology has found broad applications in such areas of tissue regeneration and drug delivery. More recently, coaxial electrospinning has emerged as an important technique to develop scaffolds for regenerative engineering incorporated with drug(s). However, the addition of a softer core layer leads to a reduction in mechanical properties. Here, novel robust tripolymeric triaxially electrospun fibrous scaffolds were developed with a polycaprolactone (PCL) (core layer), a 50:50 poly (lactic-co-glycolic acid) (PLGA) (sheath layer) and a gelatin (intermediate layer) with a dual drug delivery capability was developed through modified electrospinning. A sharp increase in elastic modulus after the incorporation of PCL in the core of the triaxial fibers in comparison with uniaxial PLGA (50:50) and coaxial PLGA (50:50) (sheath)-gelatin (core) fibers was observed. Thermal analysis of the fibrous scaffolds revealed an interaction between the core-intermediate and sheath-intermediate layers of the triaxial fibers contributing to the higher tensile modulus. A simultaneous dual release of model small molecule Rhodamine B (RhB) and model protein Fluorescein isothiocynate (FITC) Bovine Serum Albumin (BSA) conjugate incorporated in the sheath and intermediate layers of triaxial fibers was achieved. The tripolymeric, triaxial electrospun systems were seen to be ideal for the support of mesenchymal stem cell growth, as shrinkage of fibers normally found with conventional electrospun systems was minimized. These tripolymeric triaxial electrospun fibers that are biomechanically competent, biocompatible, and capable of dual drug release are designed for regenerative engineering and drug delivery applications.

中文翻译：

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用于双药物递送应用的三聚合三轴电纺纤维基体的开发。

自从Laurencin及其同事在开发用于生物医学目的的聚合物静电纺丝方面的第一项工作以来，静电纺丝技术的使用已在组织再生和药物输送等领域得到了广泛的应用。最近，同轴电纺丝已经成为开发用于与药物结合的再生工程的支架的重要技术。然而，添加较软的芯层导致机械性能降低。在这里，开发了新型坚固的三聚合三轴电纺纤维支架，该支架具有聚己内酯（PCL）（芯层），50：50聚（乳酸-乙醇酸）（鞘层）和明胶（中间层），通过改进的静电纺丝技术开发了双重药物输送能力。与单轴PLGA（50:50）和同轴PLGA（50:50）（皮）-明胶（芯）纤维相比，在三轴纤维的芯中掺入PCL后的弹性模量急剧增加。纤维支架的热分析显示三轴纤维的芯-中间层和皮-中间层之间的相互作用有助于较高的拉伸模量。实现了模型小分子若丹明B（RhB）和模型蛋白异硫氰酸荧光素（FITC）牛血清白蛋白（BSA）共轭物的双重双重释放，该结合物掺入了三轴纤维的皮层和中间层。三聚体，三轴电纺丝系统被认为是支持间充质干细胞生长的理想选择，因为通常在常规电纺丝系统中发现的纤维收缩率最小。