Abstract Colorimetric assay of melanogenesis inhibition was demonstrated with polyethylene glycol tetraacrylate (PEG-4A) hydrogel structures embedding melanocyte cells. The cell laden PEG-4A hydrogel structure was prepared by digital light processing printing using biocompatible visible range of light. The melanocyte in PEG-4A hydrogel structure showed a high cell compatibility and expressed melanin changing a color of hydrogel to black. The inhibition of melanin expression was evaluated by injecting a different concentration of arbutin and kojic acid to the cell laden hydrogel structure in a 3D printed microfluidic chip. The tyrosinase inhibitors prevented a color change of the hydrogel pad according to the dosage showing a similar detecting efficiency to a commercial melanogenesis assay kit.

中文翻译：

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使用数字光处理印刷制造的载有黑色素细胞的水凝胶对酪氨酸酶抑制的比色测定

摘要 用聚乙二醇四丙烯酸酯 (PEG-4A) 水凝胶结构包埋黑色素细胞，证明了黑色素生成抑制的比色测定。负载细胞的 PEG-4A 水凝胶结构是通过使用生物相容性可见光范围的数字光处理印刷制备的。PEG-4A 水凝胶结构中的黑色素细胞显示出高细胞相容性并表达黑色素，使水凝胶的颜色变为黑色。通过将不同浓度的熊果苷和曲酸注射到 3D 打印微流控芯片中充满细胞的水凝胶结构中来评估黑色素表达的抑制作用。酪氨酸酶抑制剂根据剂量阻止了水凝胶垫的颜色变化，显示出与商业黑素生成测定试剂盒相似的检测效率。