显示样式:     当前期刊: Bioelectrochemistry    加入关注       排序: 导出
我的关注
我的收藏
您暂时未登录!
登录
  • Keratinocyte electrotaxis induced by physiological pulsed direct current electric fields
    Bioelectrochemistry (IF 3.789) Pub Date : 2019-02-14
    Xi Ren, Huanbo Sun, Jie Liu, Xiaowei Guo, Jingzhuo Huang, Xupin Jiang, Yiming Zhang, Yuesheng Huang, Dongli Fan, Jiaping Zhang

    Endogenous electric fields (EFs) direct the migration (electrotaxis) of keratinocytes in skin wounds, and the exogenous application of EFs may therefore improve wound healing, but the potential benefits are limited by the side effects of constant direct current (DC) passing through tissues. In contrast, with pulsed DC (characterized by intermittent output), parameters can be adjusted to minimize the adverse effects of electric currents. However, it remains unknown whether pulsed DC can reliably induce keratinocyte electrotaxis. In this study, using primary keratinocytes in an electrotaxis chamber, we found that a pulsed DCEF at physiological strength (EF = 150 mV/mm, duty cycle = 60%, frequency = 0.1 Hz) could induce robust electrotaxis. This effect was dependent on both voltage and duty cycle, but not on frequency. As predicted, fewer electrochemical reactions and cytotoxic reactions were detected with pulsed DCEF than with constant DCEF. In summary, we here demonstrate for the first time, that pulsed DCEF can trigger keratinocyte electrotaxis comparable to that induced by constant DCEF, while minimizing the electrochemical side effects. These findings support the future development of a pulsed DCEF device to improve wound healing in human patients.

    更新日期:2019-02-15
  • 更新日期:2019-02-14
  • A sensitive amperometric AChE-biosensor for organophosphate pesticides detection based on conjugated polymer and Ag-rGO-NH2 nanocomposite
    Bioelectrochemistry (IF 3.789) Pub Date : 2019-02-13
    Pan Zhang, Tingting Sun, Shengzhong Rong, Dongdong Zeng, Hongwei Yu, Ze Zhang, Dong Chang, Hongzhi Pan
    更新日期:2019-02-14
  • 更新日期:2019-02-14
  • A reduced graphene oxide-titanium dioxide nanocomposite based electrochemical aptasensor for rapid and sensitive detection of Salmonella enterica
    Bioelectrochemistry (IF 3.789) Pub Date : 2019-02-11
    Shalini Muniandy, Swe Jyan Teh, Jimmy Nelson Appaturi, Kwai Lin Thong, Chin Wei Lai, Fatimah Ibrahim, Bey Fen Leo

    Recent foodborne outbreaks in multiple locations necessitate the continuous development of highly sensitive and specific biosensors that offer rapid detection of foodborne biological hazards. This work focuses on the development of a reduced graphene oxide‑titanium dioxide (rGO-TiO2) nanocomposite based aptasensor to detect Salmonella enterica serovar Typhimurium. A label-free aptamer was immobilized on a rGO-TiO2 nanocomposite matrix through electrostatic interactions. The changes in electrical conductivity on the electrode surface were evaluated using electroanalytical methods. DNA aptamer adsorbed on the rGO-TiO2 surface bound to the bacterial cells at the electrode interface causing a physical barrier inhibiting the electron transfer. This interaction decreased the DPV signal of the electrode proportional to decreasing concentrations of the bacterial cells. The optimized aptasensor exhibited high sensitivity with a wide detection range (108 to 101 cfu mL−1), a low detection limit of 101 cfu mL−1 and good selectivity for Salmonella bacteria. This rGO-TiO2 aptasensor is an excellent biosensing platform that offers a reliable, rapid and sensitive alternative for foodborne pathogen detection.

    更新日期:2019-02-13
  • Electron transfer interpretation of the biofilm-coated anode of a microbial fuel cell and the cathode modification effects on its power
    Bioelectrochemistry (IF 3.789) Pub Date : 2019-02-08
    Yamin Yang, Chansoo Choi, Guorong Xie, Jong-Deok Park, Shao Ke, Jong-Sung Yu, Juanjuan Zhou, Bongsu Lim

    Biofilm-coated electrodes and outer cell membrane-mimicked electrodes were examined to verify an extracellular electron transfer mechanism using Marcus theory for a donor–acceptor electron transfer. Redox couple-bound membrane electrodes were prepared by impregnating redox coenzymes into Nafion films on carbon cloth electrodes. The electron transfer was believed to occur sequentially from acetate to nicotinamide adenine dinucleotide (NAD), c-type cytochrome, flavin mononucleotide (FMN) (or riboflavin (RBF)) and the anode substrate. Excellent polarisation and power density characteristics were contributed by the modification of the cathode with a high-surface-area ordered mesoporous carbon or a hollow core–mesoporous shell carbon. The maximum power density of the microbial fuel cell (MFC) could be improved by a factor of two mainly due to the accelerated electron consumption by modifying the cathode surfaces within three-dimensionally interconnected mesoporous carbon particles, and the anode was coated with a mixed culture of anaerobic bacteria.

    更新日期:2019-02-11
  • Influence of the electrode material on ROS generation and electroporation efficiency in low and high frequency nanosecond pulse range
    Bioelectrochemistry (IF 3.789) Pub Date : 2019-02-06
    Paulius Ruzgys, Vitalij Novickij, Jurij Novickij, Saulius Šatkauskas

    Electroporation is a widely-used methodology for permeabilization of cells using pulsed electric field (PEF). In this paper, we compare the electroporation efficiency in terms of molecular transport and the generated reactive oxygen species (ROS) between low (1 Hz) and high (1 MHz) frequency nanosecond range PEF bursts. We used aluminum, copper and stainless-steel electrodes and evaluated the influence of electrode material on ROS generation and electroporation. Bursts of 25 or 50 pulses of 7–14 kV/cm amplitude and 200 ns duration were applied, and the results were compared to those obtained using electroporation with pulses of equivalent energy in conventional microsecond range (100 μs × 8). It was determined that electroporation efficiency scales with ROS generation and is highly affected by the material of electrodes and by the applied pulsing protocols. We present experimental evidence that metal ions, and not the pH fronts near the electrodes, play a major role in generation of ROS during electroporation.

    更新日期:2019-02-06
  • Biosensor for direct bioelectrocatalysis detection of nitric oxide using nitric oxide reductase incorporated in carboxylated single-walled carbon nanotubes/lipidic 3 bilayer nanocomposite
    Bioelectrochemistry (IF 3.789) Pub Date : 2019-02-05
    Filipa O. Gomes, Luísa B. Maia, Joana A. Loureiro, Maria Carmo Pereira, Cristina Delerue-Matos, Isabel Moura, José J.G. Moura, Simone Morais

    An enzymatic biosensor based on nitric oxide reductase (NOR; purified from Marinobacter hydrocarbonoclasticus) was developed for nitric oxide (NO) detection. The biosensor was prepared by deposition onto a pyrolytic graphite electrode (PGE) of a nanocomposite constituted by carboxylated single-walled carbon nanotubes (SWCNTs), a lipidic bilayer [1,2-di-(9Z-octadecenoyl)-sn-glycero-3-phosphoethanolamine (DOPE), 1,2-di-(9Z-octadecenoyl)-3-trimethylammonium-propane (DOTAP), 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-polyethylene glycol (DSPE-PEG)] and NOR. NOR direct electron transfer and NO bioelectrocatalysis were characterized by several electrochemical techniques. The biosensor development was also followed by scanning electron microscopy and Fourier transform infrared spectroscopy. Improved enzyme stability and electron transfer (1.96 × 10−4 cm.s−1 apparent rate constant) was obtained with the optimum SWCNTs/(DOPE:DOTAP:DSPE-PEG)/NOR) ratio of 4/2.5/4 (v/v/v), which biomimicked the NOR environment. The PGE/[SWCNTs/(DOPE:DOTAP:DSPE-PEG)/NOR] biosensor exhibited a low Michaelis-Menten constant (4.3 μM), wide linear range (0.44–9.09 μM), low detection limit (0.13 μM), high repeatability (4.1% RSD), reproducibility (7.0% RSD), and stability (ca. 5 weeks). Selectivity tests towards L-arginine, ascorbic acid, sodium nitrate, sodium nitrite and glucose showed that these compounds did not significantly interfere in NO biosensing (91.0 ± 9.3%–98.4 ± 5.3% recoveries). The proposed biosensor, by incorporating the benefits of biomimetic features of the phospholipid bilayer with SWCNT's inherent properties and NOR bioelectrocatalytic activity and selectivity, is a promising tool for NO.

    更新日期:2019-02-05
  • Electrochemical characterization of LHCII on graphite electrodes – Potential-dependent photoactivation and arrangement of complexes
    Bioelectrochemistry (IF 3.789) Pub Date : 2019-01-22
    Paulina Piotrowska, Magdalena Łazicka, Adriana Palińska-Saadi, Bohdan Paterczyk, Łucja Kowalewska, Joanna Grzyb, Magdalena Maj-Żurawska, Maciej Garstka
    更新日期:2019-01-23
  • Effect of alternating current and Bacillus cereus on the stress corrosion behavior and mechanism of X80 steel in a Beijing soil solution
    Bioelectrochemistry (IF 3.789) Pub Date : 2019-01-21
    Hongxia Wan, Dongdong Song, Cuiwei Du, Zhiyong Liu, Xiaogang Li

    The effects of alternating current (AC) and Bacillus cereus (B. cereus) on the stress corrosion behavior and mechanism of X80 pipeline steel were investigated in a Beijing soil solution in this study. Both AC and B. cereus can promote the generation of pits in X80 steel. B. cereus is a type of nitrate-reducing bacteria and hence nitrate reduction occurs at the bottom of the corrosion product film or the biofilm. Meanwhile, the anode accelerates the dissolution of X80 steel and generates some pits in the local working area. The synergistic effect of AC and nitrate- reducing bacteria (NRB) promotes X80 steel corrosion and increases its susceptibility to stress corrosion cracking (SCC).

    更新日期:2019-01-23
  • 更新日期:2019-01-23
  • Scalability of self-stratifying microbial fuel cell: Towards height miniaturisation
    Bioelectrochemistry (IF 3.789) Pub Date : 2019-01-09
    Xavier Alexis Walter, Carlo Santoro, John Greenman, Ioannis A. Ieropoulos

    The scalability of bioelectrochemical systems is a key parameter for their practical implementation in the real-world. Up until now, only urine-fed self-stratifying microbial fuel cells (SSM-MFCs) have been shown to be scalable in width and length with limited power density losses. For practical reasons, the present work focuses on the scalability of SSM-MFCs in the one dimension that has not yet been investigated, namely height. Three different height conditions were considered (1 cm, 2 cm and 3 cm tall electrodes). The normalised power density of the 2 cm and 3 cm conditions were similar either during the durability test under a hydraulic retention time of ≈39 h (i.e. 15.74 ± 0.99 μW.cm−3) and during the polarisation experiments (i.e. 27.79 ± 0.92 μW.cm−3). Conversely, the 1 cm condition had lower power densities of 11.23 ± 0.07 μW.cm−3 and 17.73 ± 3.94 μW.cm−3 both during the durability test and the polarisation experiment, respectively. These results confirm that SSM-MFCs can be scaled in all 3 dimensions with minimal power density losses, with a minimum height threshold for the electrode comprised between 1 cm and 2 cm.

    更新日期:2019-01-10
  • A two-phase gradual silver release mechanism from a nanostructured TiAlV surface as a possible antibacterial modification in implants
    Bioelectrochemistry (IF 3.789) Pub Date : 2019-01-08
    Eva Pruchova, Michaela Kosova, Jaroslav Fojt, Petra Jarolimova, Eva Jablonska, Vojtech Hybasek, Ludek Joska

    Titanium biomaterials are widely used in the medical field due to their biocompatibility and excellent corrosion and mechanical resistance. However, these materials have no antibacterial properties. To obtain an antibacterial active surface, a nanostructure of Ti6Al4V alloy was created. This specific nanostructure contained nanotubes and micro-cavities and was used as a substrate for silver anchoring. The electrochemical approach to silver reduction was studied. It is a common approach for silver deposition and in this work, inhomogeneities in the nanostructure were used as a preferential area for silver localisation. The galvanostatic regimen of deposition allowed for a technically quantitative process and the required silver placement. The experimental conditions used enabled testing and silver dissolution rate evaluation within a reasonable time span. Based on the corrosion and analytical results (EDS, XPS and ICP-MS), a two-phase silver release mechanism was confirmed. The openings of the individual nanotubes were filled with silver nanoparticles, whose release was relatively fast. By contrast, the silver anchored inside the cavities allowed the silver to release gradually. Antibacterial efficiency against Staphylococcus aureus and Escherichia coli was successfully demonstrated. Cytotoxicity testing with murine fibroblasts showed cell metabolic activity far above the normative limit of 70%.

    更新日期:2019-01-09
  • Electrochemical evidence for direct interspecies electron transfer between Geobacter sulfurreducens and Prosthecochloris aestuarii
    Bioelectrochemistry (IF 3.789) Pub Date : 2019-01-05
    Lingyan Huang, Xing Liu, Jiahuang Tang, Linpeng Yu, Shungui Zhou

    The syntrophic photosynthesis via direct interspecies electron transfer (DIET) between Geobacter sulfurreducens and Prosthecochloris aestuarii has opened a new paradigm of microbial phototrophy. However, it is still unknown whether this photosynthetic DIET can be mediated by an electrical conductor. Here we report first the photosynthetic DIET in a two-chamber microbial fuel cell (photo-MFC). The photo-MFC worked well and generated a maximum current of 0.6 mA/m2, which validated photosynthetic DIET via the titanium wire. Confocal laser scanning microscopy showed that G. sulfurreducens and P. aestuarii colonized the anode and cathode, respectively. P. aestuarii accepted extracellular electrons from G. sulfurreducens bioanodes under illumination at a current density of 0.6 mA/m2 (normalized to the cathode surface area), which could not be produced in the dark. Such a light-dependent electron uptake from solid electrodes by P. aestuarii was closely related with the presence of CO2, suggesting that P. aestuarii utilized extracellular electrons for photosynthetic CO2 reduction. Electrochemical in situ Fourier transform infrared (FTIR) spectroscopy revealed that certain outer membrane components of the two strains were involved in the DIET process. These results implied photosynthetic DIET can be mediated by electrically conductive materials in natural environments.

    更新日期:2019-01-06
  • Electrochemical, spectroscopic and molecular docking studies on the interaction of calcium channel blockers with dsDNA
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-12-28
    Suniya Shahzad, Burcu Dogan-Topal, Leyla Karadurmus, Mehmet Gokhan Caglayan, Tuğba Taskin Tok, Bengi Uslu, Afzal Shah, Sibel A. Ozkan

    This study presents evaluation of the possible interaction mechanism between calf thymus dsDNA and three calcium antagonists; nifedipine, lercanidipine and amlodipine. The interactions between Nifedipine-dsDNA and Lercanidipine-dsDNA were investigated by differential pulse voltammetry using two different interaction methods; at the dsDNA-electrochemical biosensor surface and in bulk incubated solution. Amlodipine was used as model drug in bulk incubated solution. The decrease in the peak current of guanine and adenine were used as an indicator for confirmation of the interaction event in acetate buffer of pH 4.70. In bulk incubated solution, after interaction with Nifedipine and Amlodipine the guanine signal was almost disappeared. At the dsDNA modified glassy carbon electrode surface, the peak currents of guanine and adenine were decreased while Nifedipine and Lercanidipine interacts with DNA. The interactions between Nifedipine-dsDNA and Lercanidipine-dsDNA were further studied by UV–Vis absorption spectroscopy which indicates the intermolecular interaction between these drugs and ds-DNA can be mainly through hydrogen bonding and van der Waals forces. Molecular docking calculations shown that the AMP-1-2, NDP and LDP-1-2-ctDNA having groove binding. Beside spectral data, docking studies elicited that AMP-1-2, NDP and LDP-1-2 complexes have different interaction and conformation trends to target (ctDNA).

    更新日期:2019-01-01
  • Microbial phyto-power systems – A sustainable integration of phytoremediation and microbial fuel cells
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-12-28
    Beenish Saba, Mohsin Khan, Ann D. Christy, Birthe Veno Kjellerup

    Constructed wetland microbial fuel cells (CW-MFCs) or phyto-power systems are integrated bioelectrochemical systems (BES) that can sustainably harvest electricity from the anaerobic respiration of rhizospheric bacteria. This integration of techniques shows a promise in phytoremediation of wastewater along with bioenergy generation. In CW-MFCs, electrons harvested in anaerobic respiration of bacteria proliferating in the rhizospheric zone are electrochemically coupled with electron acceptors at the aerobic cathode submersed in water. Use of indigenous non-food plants in CW-MFCs has gained increasing interest primarily due to high yield of biomass that can be applied for other bioenergy purposes and bioaccumulation of pollutants. Furthermore, CW-MFCs can provide other benefits such as wastewater treatment, carbon dioxide assimilation, power generation and air purification. Microbial interaction with plant roots (rhizosphere), isolated species from the phyto-systems, with soil particles and pollutants are reviewed in this paper. In addition, successful applications of CW-MFCs are discussed with focus on power generation, the role of plant-microbe interactions as well as evaluating the critical operational parameters and their effect on power generation output efficiency.

    更新日期:2019-01-01
  • A multi-functional minimally-disruptive portable electrochemical system based on yeast/Co3O4/Au/SPEs for blood lead (II) measurement
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-12-21
    Jing Nie, Bin He, Yu-jiao Zang, Wei Yin, Liang-ri Han, Wen-fei Li, Chang-jun Hou, Dan-qun Huo, Mei Yang, Huan-bao Fa

    A minimally-disruptive portable electrochemical system is constructed by combining a hand-held syringe as reservoir with disposable screen-printed electrodes (SPEs) modified with a simple and efficient yeast/Co3O4/Au material for lead determination by a square-wave voltammetry (SWV) method. Not only can it preserve the operation and advantages of the conventional electrochemical procedure, but it also integrates sampling, filtering and analysis to make the determination of lead convenient and effective at higher and lower concentration levels. This is the first report of a microbial biosensor based on active yeast crosslinked to Co3O4/Au particles using glutaraldehyde as the crosslinking agent. The determination process is simplified by introducing a fiber filter and takes only 150 s with the developed system, which illustrates its simplicity, speed and detection accuracy. Also, the design shows a wide log-linear dynamic range (LDR) from 10−8 to 10−14 g·L−1, with a limit of detection (LOD) of 3.45 × 10−15 g·L−1 (S/N = 3). Additionally, the proposed system was used to determine lead in blood samples, which demonstrated the potential of this biosensor for use in practical applications. Furthermore, this study provides a basis for the development of microscale blood devices for lead measurement.

    更新日期:2018-12-21
  • β-Lactoglobulin as a potential carrier for bioactive molecules
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-12-20
    S. Świątek, P. Komorek, G. Turner, B. Jachimska

    In this study, the interaction and binding behavior of anesthetic tetracaine (TET) with bovine β-lactoglobulin (LGB) isoform A and a mixture of isoforms A and B were investigated under varying environmental conditions (pH, ionic strength, concentration, LGB-TET complex molar ratio). A wide range of analytical techniques (dynamic light scattering (DLS), electrophoretic mobility, UV–Vis spectroscopy, circular dichroism (CD), quartz crystal microbalance (QCM-D) were used to analyze the physicochemical properties of the complexes in bulk solution and on the surface of gold. The experiments revealed that TET, which is amphiphilic, could bind with LGB not only in the β-barrel but also onto the surface. The zeta potential of the LGB becomes more positively charged upon interaction with TET due to electrostatic interaction of the amino group present in the TET structure. Changes in the zeta potential values are mostly visible above pH 6 for all tested systems. CD spectra show that interaction with the ligand does not change the secondary structure of the protein. The physicochemical properties of LGB-TET complex were examined in an adsorbed state on a gold surface using the QCM-D method. Additionally, molecular docking was used to evaluate the most likely binding site for TET with LGB.

    更新日期:2018-12-20
  • Regenerated silk fibroin membranes as separators for transparent microbial fuel cells
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-12-17
    Grzegorz Pasternak, Yuejiao Yang, Bruno Bosquiroli Santos, Federico Brunello, Martin M. Hanczyc, Antonella Motta

    In recent years novel applications of bioelectrochemical systems are exemplified by phototrophic biocathodes, biocompatible enzymatic fuel cells and biodegradable microbial fuel cells (MFCs). Herein, transparent silk fibroin membranes (SFM) with various fibroin content (2%, 4% and 8%) were synthesised and employed as separators in MFCs and compared with standard cation exchange membranes (CEM) as a control. The highest real-time power performance of thin-film SFM was reached by 2%-SFM separators: 25.7 ± 7.4 μW, which corresponds to 68% of the performance of the CEM separators (37.7 ± 3.1 μW). Similarly, 2%-SFM revealed the highest coulombic efficiency of 6.65 ± 1.90%, 74% of the CEM efficiency. Current for 2%-SFM reached 0.25 ± 0.03 mA (86% of CEM control). Decrease of power output was observed after 23 days for 8% and 4% and was a consequence of deterioration of SFMs, determined by physical, chemical and biological studies. This is the first time that economical and transparent silk fibroin polymers were successfully employed in MFCs.

    更新日期:2018-12-18
  • Interfacial electron transfer between Geobacter sulfurreducens and gold electrodes via carboxylate-alkanethiol linkers: Effects of the linker length
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-12-12
    M. Füeg, Z. Borjas, M. Estevez-Canales, A. Esteve-Núñez, I.V. Pobelov, P. Broekmann, A. Kuzume

    Geobacter sulfurreducens (Gs) attachment and biofilm formation on self-assembled monolayers (SAMs) of carboxyl-terminated alkanethiol linkers with varied chain length on gold (Au) was investigated by electrochemical and microscopic methods to elucidate the effect of the surface modification on the current production efficiency of Gs cells and biofilms. At the initial stage of the cell attachment, the electrochemical activity of Gs cells at a submonolayer coverage on the SAM-Au surface was independent of the linker length. Subsequently, multiple potential cyclings indicated that longer linkers provided more biocompatible conditions for Gs cells than shorter ones. For Gs biofilms, on the other hand, the turnover current decreased exponentially with the linker length. During the biofilm formation, bacteria need to adjust from the initial planktonic state to an electrode-respiring state, which was triggered by a strong electrochemical stress found for shorter linkers, resulting in the formation of mature biofilms. Our results suggest that the initial cell attachment and the biofilm formation are two inherently different processes. Therefore, the effects of linker molecules, electron transfer efficiency and biocompatibility, must be explored simultaneously to understand both processes to increase the current production of electrogenic microorganisms in microbial fuel cells.

    更新日期:2018-12-12
  • Influence of the current density in moderate pulsed electric fields on P. putida F1 eradication
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-12-11
    Efrat Emanuel, Pogreb Roman, Rivka Cahan

    Eradication of P. putida F1 was investigated as a function of current density in pulsed electric fields of 6.7, 4, 2.8, 2 and 1 kV/cm. The pulse numbers were 200, 2000, 5000 and 10,000 and were performed by a series of trains of 500 pulses (except for the 200 pulses). The frequency was 100 Hz and pulse durations were 10 μs or 20 μs as indicated for each experiment. The current density range was 0.02 ± 0.01 to 5.2 ± 0.5 Acm−2. A clear tendency for increasing bacterial death was found as a result of increasing the current density in each of the tested electric field strengths. The total bacterial eradication when the electric field was reduced from 4 to 1 kV/cm was obtained at a higher current density, 2 ± 0.2 and 5.2 ± 0.5 Acm−2, respectively. Increasing the current density led to higher cell permeability and larger bacteria size. The percentage of propidium iodide permeability in an electric field of 1 kV/cm at a current density of 5.2 ± 0.5 Acm−2 was 65 ± 0.3% compared to the control that was only 10 ± 0.9%. The cell size at 1 kV/cm in a current density of 5.2 ± 0.5 Acm−2 was about 3-fold higher compared to untreated cells. To the best of our knowledge, this is the first study that evaluated the influence of increasing current density on bacterial eradication in moderate electric fields.

    更新日期:2018-12-11
  • Cytochrome P450 genes play central roles in transcriptional response by keratinocytes to a high-voltage alternating current electric field
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-12-11
    Masayo Aoki, Noriko M. Matsumoto, Yuri Okubo, Rei Ogawa

    The endogenous electric field (EF) of skin wounds plays an important role in the biological processes that underlie wound healing. Treatments that modulate wound-EFs promote healing. However, the mechanism(s) that underlie this effect remain unclear. Agilent-based microarrays were used to determine the transcriptomes of the keratinocyte line HaCaT, normal human dermal fibroblasts, and the human dermal endothelial cell line HMEC-1 before and after high-voltage alternating current (AC)-EF (14,000 V, 90 Hz) treatment. The keratinocytes had the most genes whose transcription was altered by EF. They included the cytochrome P450 (CYP) genes CYP1A1 and CYP1B1, HMOX1, EREG, DUSP5, and SLC7A11 (all upregulated), and DOCK8, ABCC6, and CYP26A1 (all downregulated). As shown by transcriptional-network analysis, all three CYP genes played central roles in the EF-induced changes in keratinocyte transcriptome. To the best of our knowledge, this is the first study that demonstrates that CYP genes play a key role in the transcriptional responses of human keratinocytes to EF treatment. Further investigations into the effects of EF on wound healing, aging, and regenerative medicine are likely to yield promising results.

    更新日期:2018-12-11
  • Corrosion behavior of X80 pipeline steel in the presence of Brevibacterium halotolerans in Beijing soil
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-12-05
    Zhong Li, Hongxia Wan, Dongdong Song, Xiaodong Liu, Ziyu Li, Cuiwei Du

    In this study, the corrosion behavior of X80 pipeline steel in the presence of Brevibacterium halotolerans (B. halotolerans) was investigated by surface analysis and electrochemical measurements. Results show that B. halotolerans can attach to the surface of X80 steel, and the entire surface is covered with B. halotolerans. The corrosion products induced by B. halotolerans were FeOOH, Fe2O3 and FeSO4. X80 steel corrosion was accelerated in the presence of B. halotolerans and was susceptible to pitting corrosion. The formation of pitting corrosion could be due that the B. halotolerans oxidization of elemental iron in X80 steel to either obtain electrons from the reduction of nitrate in the underlying biofilm or in the damaged corrosion product film.

    更新日期:2018-12-06
  • Microbial electrochemical system for the phenol degradation using alternating current: Metabolic pathway study
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-12-05
    Zohreh Moghiseh, Abbas Rezaee, Somayyeh Dehghani, Ali Esrafili

    The present study was conducted to investigate the effect of alternating current (AC) on phenol removal in a microbial electrochemical system (MES) and determine its by-products. The bioreactor used for this purpose operates in the batch mode supplied with an AC power supply. The factors stimulating this process including frequency, applied voltage, duty cycle, carbon to nitrogen ratio, and the initial phenol concentration were investigated. The optimum operating conditions of the bioreactor were obtained at 5 Hz frequency, 0.4 peak-to-peak voltage (Vpp), C0 = 100 mg.L−1 phenol, pH = 7, C/N = 1, and the sine wave. Phenol was completely degraded under the optimum operating conditions for 2 h. The GC–MS analysis showed the presence of carboxylic acid, oxalic acid, and propionic acid. It was observed that the generated by-products are non-toxic and phenol is completely removed to nontoxic compounds. The results show that under optimum conditions, using an alternating current, the proposed system generated low-hazard byproducts with a low energy consumption.

    更新日期:2018-12-06
  • Electrochemical behavior of titanium and platinum in dicarboxilic amino acids solution
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-12-04
    E.A. Kornyushova, A.V. Kashevskii, K.Y. Arsent'ev, B.G. Pushkarev, S.B. Nikiforov, A.Y. Safronov

    Titanium and platinum samples as components of bimetallic implants for the osteoregeneration process have been modified in solutions modeling biological systems and studied by means of cyclic voltammetry, electrochemical impedance spectroscopy and scanning probe microscopy. While aspartic and glutamic acids did not adsorb significantly on platinum in the potential region investigated, the presence of the amino acids affects oxide layer growth on the titanium surface under anodic polarization. The two studied amino acids behave differently on the titanium electrode surface due to differences in adsorption modes of these substances. The adsorption of the glutamic acid depends on the polarization potential to a large extent, and most of quantitative adsorption characteristics (EIS data, the surface roughness) undergo drastic change at the polarization potential value of 750 mV (vs. Ag/AgCl) in the presence of this substance. Equivalent circuit modeling of the surface processes has been carried out, and a scheme for aspartic and glutamic acid adsorption onto the titanium surface has proposed.

    更新日期:2018-12-05
  • Cytotoxicity studies of quantum dots with the electroporation method
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-12-01
    Sandra Skorupska, Ilona Grabowska-Jadach

    In this study, the cytotoxicity of CdTe quantum dots (QDs) of various dimensions was examined using the electroporation method. The influence of the size of QDs on normal and tumour cell viability after 24 h of incubation with nanomaterials was examined. The three human cell lines were chosen for the tests: A549 (a tumour cell line derived from the lung), MRC-5 (normal fibroblasts from the lung) and HaCaT (normal keratinocytes from the skin). Accordingly, we modelled the effect of nanocrystals on various human tissues because nanoparticles can be introduced into an organism through different routes. We were also able to study which cells are more sensitive to nanoparticles: normal or tumour cells. The nanoparticles were introduced into cells through pores in the cell membranes that were generated by electrical pulses. The effectiveness of introducing nanocrystals into cells was determined as a function of the nanocrystal dimensions and accumulation locations. Moreover, the cytotoxicity of quantum dots was tested, and cell viability after electroporation was evaluated. We also investigated whether the introduced nanocrystals released cadmium ions.

    更新日期:2018-12-01
  • A comparison of microbial fuel cell and microbial electrolysis cell biosensors for real-time environmental monitoring
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-12-01
    Ademola Adekunle, Vijaya Raghavan, Boris Tartakovsky

    This study compares the biosensing performance of a microbial fuel cell (MFC) and a microbial electrolysis cell (MEC). Initial tests provided a qualitative comparison of MFC and MEC currents after the anode compartment liquid (anolyte) was spiked with acetate, or sulphates of NH4+, Na+, Mg2+, Fe2+, or a fertilizer solution. Current measurements showed that the MFC sensor had a faster response time, higher sensitivity, and faster recovery time after the spike. Following the spike tests, the MFC and MEC were operated in a continuous flow mode at several influent concentrations of acetate, and sulphates of NH4+, Na+, and Fe2+. The continuous flow tests confirmed the better performance of the MFC sensor, which was selected for further experiments. Two MFC sensors were used for real-time (on-line) COD measurements of brewery wastewater. Regression analysis showed a strong correlation between the MFC power output and COD concentrations in the anode compartment with a coefficient of determination (R2) of 0.97. Overall, results of this study suggest that an MFC-based sensor can be successfully used as a simple and cost-efficient real-time monitoring tool.

    更新日期:2018-12-01
  • A sandwich-type electrochemical immunosensor based on RhPt NDs/NH2-GS and Au NPs/PPy NS for quantitative detection hepatitis B surface antigen
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-12-01
    Fubin Pei, Ping Wang, Enhui Ma, Qingshan Yang, Haoxuan Yu, Chunxiao Gao, Yueyun Li, Qing Liu, Yunhui Dong

    In this work, a sandwich-type electrochemical immunosensor was fabricated to quantitatively detect hepatitis B surface antigen (HBsAg). The immunosensor was based on Rh core and Pt shell nanodendrites loaded onto amino group functionalized graphene nanosheet (RhPt NDs/NH2-GS) as label and gold nanoparticles loaded onto polypyrrole nanosheet (Au NPs/PPy NS) as platform. RhPt NDs with abundant catalytic active sites because of the branched core-shell structure, RhPt NDs/NH2-GS as the label displayed high catalytic activity, amplifying the current signal of the immunosensor. Additionally, Au NPs/PPy NS enhanced the electron transfer and provided a good microenvironment to immobilize antibodies effectively, thus improving the sensitivity of the immunosensor. Based on above advantages, the immunosensor emerged a linear concentration ranging from 0.0005 to 10 ng/mL, a low detection limit of 166 fg/mL for HBsAg (S/N = 3) and good stability, selectivity, reproducibility. Furthermore, the satisfactory accuracy in analysis of actual serum samples implied the immunosensor had promising prospect in clinical analysis applications.

    更新日期:2018-12-01
  • A novel operational strategy to enhance wastewater treatment with dual-anode assembled microbial desalination cell
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-12-01
    Fubin Liu, Lisheng Wang, Kuichang Zuo, Shuai Luo, Xiaoyuan Zhang, Peng Liang, Xia Huang

    This study introduced a novel dual-anode assembled microbial desalination cell to enhance the performance of domestic wastewater treatment. Two parallel units were fabricated with two anodes and one cathode, which is separated by two ion exchange membrane stacks. A hollow fiber membrane module was inserted in the cathode to intercept suspended solids and microbes. Based on preliminary experiments where synthetic wastewater was utilized, anode hydraulic retention time of 10 h and cathode aeration rate of 0.16 m3/h were chosen as the operating conditions. By innovatively connecting four membrane stacks in cascades, which multiplied flow rate without adding extra circulation pumps, the desalination rate of the system was improved 214.8% compared with single membrane stack mode. When modified domestic wastewater was applied, the average removal efficiencies of chemical oxygen demand, ammonia nitrogen, total nitrogen and total phosphorous reached 96.9%, 99.0%, 98.0% and 98.3%, respectively.

    更新日期:2018-12-01
  • Synthesis of anticorrosion nanohybrid films based on bioinspired dopamine, L-cys/CNT@PDA through self-assembly on 304 stainless steel in 3.5% NaCl
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-11-30
    Fatemeh Elmi, Elahe Valipour, Shahram Ghasemi

    Nanohybrid films containing multiwalled carbon nanotubes (MWCNTs) were successfully coated on 304-stainless steel (304ss) for anti-corrosion use. The nanocompositewas made by a self-assembly of poly (dopamine), wrapped with MWCNTs (CNT@PDA) through a mussel inspired method. In order to enhance the corrosion protection, an inner layer of L-cysteine, an adhesive amino acid to 304ss surface through thiol (-SH) functional group were constructed through a dip-coating process. Potentiodynamic polarization measurements and electrochemical impedance spectroscopy revealed that the double nano-layer could act as a noble anticorrosive coating in 3.5% NaCl, which was assigned to the hydrophobicity, robustness, and dense double layer coating.

    更新日期:2018-11-30
  • The contribution of lipid peroxidation to membrane permeability in electropermeabilization: A molecular dynamics study
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-08-04
    Lea Rems, Marilyne Viano, Marina A. Kasimova, Damijan Miklavčič, Mounir Tarek

    Electroporation or electropermeabilization is a technique that enables transient increase in the cell membrane permeability by exposing cells to pulsed electric field. However, the molecular mechanisms of the long-lived cell membrane permeability, which persists on the minutes time scale after the pulse treatment, remain elusive. Experimental studies have suggested that lipid peroxidation could present a mechanism of this prolonged membrane permeabilization. In this study we make the first important step in quantifying the possible contribution of lipid peroxidation to electropermeabilization. We use free energy calculations to quantify the permeability and conductance of bilayers, containing an increasing percentage of hydroperoxide lipid derivatives, to sodium and chloride ions. We then compare our calculations to experimental measurements on electropermeabilized cells. Our results show that the permeability and conductance increase dramatically by several orders of magnitude in peroxidized bilayers. Yet this increase is not sufficient to reasonably account for the entire range of experimental measurements. Nevertheless, lipid peroxidation might be considered an important mechanism of prolonged membrane permeabilization, if exposure of cells to high voltage electric pulses leads to secondary lipid peroxidation products. Our analysis calls for experimental studies, which will determine the type and amount of lipid peroxidation products in electropermeabilized cell membranes.

    更新日期:2018-11-29
  • Cell-seeded 3D scaffolds as in vitro models for electroporation
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-08-30
    Paola Brun, Monica Dettin, Luca Giovanni Campana, Fabrizio Dughiero, Paolo Sgarbossa, Clara Bernardello, Anna Lisa Tosi, Annj Zamuner, Elisabetta Sieni

    Electroporation of cells is usually studied using cell suspensions or monolayer cultures. 3D scaffolds for cell culture have been recently designed in order to reproduce in vitro the complex and multifactorial environment experimented in vivo by cells. In fact, it is well known that 2D cell cultures are not able to simulate the complex interactions between the cells and their extracellular matrix (ECM). Recently, some examples of 3D models, like spheroids, have been investigated also in the electroporation field. Spheroids have been proposed in electrochemotherapy (ECT) studies to mimic tumor in vivo conditions: they are easy-to-handle 3D models but their sensitivity to electric field pulses depends from their diameter and, more interestingly, despite being relevant for intercellular junctions, they are not so much so for cell-ECM interactions. In this work, we propose a 3D macroscopic myxoid matrix for cell culture that would mimic the in vivo environment of myxoid stroma tumors. The myxoid stroma consists of abundant basic substances with large amounts of glycosaminoglycans (hyaluronic acid) and proteoglycans, poor collagen fibers and no elastin content. In the proposed approach, tumor cells seeded on 3D scaffolds mimic of myxoid stroma can establish both cell-cell and cell-ECM 3D interactions. The MCF7 cells (human breast adenocarcinoma cell line) were seeded in complete culture medium. Cell cultures were incubated at 37 °C for either 24 h, 3 days or 7 day. Some samples were used to assess cell vitality using 3-(4,5-dimethylthiazolyl-2)-2,5-diphenyltetrazolium bromide (MTT) test and others for electroporation tests and for histopathological analysis. The electroporation has been verified by the fluorescent dye Propidium cellular uptake. The proposed myxoid stroma scaffold induces cell proliferation and shows fibrous structures produced by cells, the concentration of which increases with culture time. The proposed matrix will be used for further investigations as a new scaffold for cell culture. Tumor cells grown into these new scaffolds will be used to evaluate electroporation including the stroma effect.

    更新日期:2018-11-29
  • Electroanalytical characterization of the direct Marinobacter hydrocarbonoclasticus nitric oxide reductase-catalysed nitric oxide and dioxygen reduction
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-08-21
    Filipa O. Gomes, Luísa B. Maia, Cristina Cordas, Isabel Moura, Cristina Delerue-Matos, José J.G. Moura, Simone Morais

    Understanding the direct electron transfer processes between redox proteins and electrode surface is fundamental to understand the proteins mechanistic properties and for development of novel biosensors. In this study, nitric oxide reductase (NOR) extracted from Marinobacter hydrocarbonoclasticus bacteria was adsorbed onto a pyrolytic graphite electrode (PGE) to develop an unmediated enzymatic biosensor (PGE/NOR)) for characterization of NOR direct electrochemical behaviour and NOR electroanalytical features towards NO and O2. Square-wave voltammetry showed the reduction potential of all the four NOR redox centers: 0.095 ± 0.002, −0.108 ± 0.008, −0.328 ± 0.001 and −0.635 ± 0.004 V vs. SCE for heme c, heme b, heme b3 and non-heme FeB, respectively. The determined sensitivity (−4.00 × 10−8 ± 1.84 × 10−9 A/μM and - 2.71 × 10−8 ± 1.44 × 10−9 A/μM for NO and O2, respectively), limit of detection (0.5 μM for NO and 1.0 μM for O2) and the Michaelis Menten constant (2.1 and 7.0 μM for NO and O2, respectively) corroborated the higher affinity of NOR for its natural substrate (NO). No significant interference on sensitivity towards NO was perceived in the presence of O2, while the O2 reduction was markedly and negatively impacted (3.6 times lower sensitivity) by the presence of NO. These results clearly demonstrate the high potential of NOR for the design of innovative NO biosensors.

    更新日期:2018-11-29
  • Cellobiose dehydrogenase hosted in lipidic cubic phase to improve catalytic activity and stability
    Bioelectrochemistry (IF 3.789) Pub Date : 2017-10-13
    Valentina Grippo, Su Ma, Roland Ludwig, Lo Gorton, Renata Bilewicz

    Lipidic cubic phase systems (LCPs) are excellent carriers for immobilized enzymes due to their biocompatibility and well-defined nanoporous structure. Lipidic cubic phases act as a convenient matrix to incorporate enzymes and hold them in the vicinity of electrode surfaces in their fully active forms. Corynascus thermophilus cellobiose dehydrogenase (CtCDH) was trapped in a monoolein cubic phase, which increased not only its stability, but also its catalytic performance with both enhanced mediated and direct electron transfer with electrodes. For studies of mediated electron transfer, three mediators with different formal potentials (E°′) were employed: horse-heart cytochrome c (cyt c), electron acceptor active with the cytochrome domain of CtCDH, and 2,6-dichlorophenolindophenol (DCPIP) as well as hexaammineruthenium(II) chloride [Ru(NH3)Cl2] both electron acceptors with the dehydrogenase domain. Ru(NH3)Cl2, having the most negative E°′ of − 0.138 V vs. Ag | AgCl at pH 7.5, gave a catalytic current for lactose oxidation of 32.10 μA cm− 2 in MOPS buffer at pH 7.5. The process carried out in the same solution but under direct electron conditions transfer resulted in a catalytic current of 9.22 μA cm− 2. Electrodes covered with CtCDH in a LCP film retained their catalytic activity after 28 days showing a slightly increased current density after 6 days.

    更新日期:2018-11-29
  • Metabolic shift of Klebsiella pneumoniae L17 by electrode-based electron transfer using glycerol in a microbial fuel cell
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-08-13
    Mi Yeon Kim, Changman Kim, Satish Kumar Ainala, Hyokwan Bae, Byong-Hun Jeon, Sunghoon Park, Jung Rae Kim
    更新日期:2018-11-29
  • Effect of isoelectric point on cheese whey wastewater treatment using a microbial electrochemical system
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-08-28
    Batoul Mohebrad, Abbas Rezaee, Beheshteh Sohrabi

    In this study, a microbial electrochemical system (MES) was employed to investigate the effect of isoelectric point (IEP) on cheese whey wastewater treatment. The experiments were carried out in a bioreactor equipped with a semicircular carbon cloth and stainless steel electrodes as anode and cathode, respectively. The effects of IEP, whey protein concentration, electrical current, and time were studied. The IEP of the whey protein was determined at pH 5.9. The optimum electrical current was obtained at 6 mA for synthetic cheese whey wastewater. The results of rotary exponential doping showed that the third structure of proteins chenges to the second structure at the IEP. The highest protein removal (98%) was obtained at pH 6. The results showed that 76%, 83%, and 98% protein removal were achieved at 2, 4, and 8 h, respectively.

    更新日期:2018-11-29
  • 更新日期:2018-11-29
  • Enhancing extracellular electron transfer efficiency and bioelectricity production by vapor polymerization Poly (3,4-ethylenedioxythiophene)/MnO2 hybrid anode
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-07-17
    Panpan Liu, Changyong Zhang, Peng Liang, Yong Jiang, Xiaoyuan Zhang, Xia Huang

    Electron transfer efficiency in electroactive biofilm is the limiting factor for bioelectricity output of bioelectrochemical system. Here, carbon felt (CF) is coated with manganese dioxide (MnO2) which acts as electron mediator in electroactive biofilm. A wrapping layer of conducting Poly 3,4-ethylenedioxythiophene is developed to protect the MnO2 and enhance electron transfer efficiency of MnO2 mediator. The hybrid bioanode (PEDOT/MnO2/CF bioanode) delivered the highest electron transfer efficiency (6.3 × 10−9 mol cm−2 s−1/2) and the highest capacitance of 4.78 F, much higher than bare CF bioanode (1.50 ± 0.04 × 10−9 mol cm−2 s−1/2 and 0.42 F). As a result, microbial fuel cells could produce a maximum power density of 1534 ± 13 mW m−2, approximately 57.7% higher than that with the bare carbon felt anode (972 ± 21 mW m−2). Possible mechanisms are proposed to help understanding the different function of the PEDOT and MnO2 on the anodic layer. This study introduces an effective method for the fabrication of high performance anode.

    更新日期:2018-11-29
  • Optimization of the droplet electroporation method for wild type Chlamydomonas reinhardtii transformation
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-11-22
    Yeong Hun Kim, Sang Gu Kwon, Seo Jun Bae, Sung Jin Park, Do Jin Im

    We performed the transformation of a wild type Chlamydomonas reinhardtii by optimizing previously developed droplet EP method. For more effective and faster optimization, we used DNA dying fluorescent molecule (Yo-Pro-1) for finding optimal EP conditions instead of using protein expression based evaluation method. By examining wider range of electrical parameter space together with the analysis of total current flow of EP process, we found optimal EP conditions. The obtained optimal EP conditions were verified by CFP transgene expression experiments. By applying the optimal EP conditions to the transformation of C. reinhardtii, we obtained transformants and analyzed them using PCR. Finally, implications and future work are discussed.

    更新日期:2018-11-24
  • Electrochemical performance and microbial community analysis in air cathode microbial fuel cells fuelled with pyroligneous liquor
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-11-19
    Guotao Sun, Kang Kang, Ling Qiu, Xiaohui Guo, Mingqiang Zhu

    Microbial fuels cells (MFCs) have been applied for the degradation of pyroligneous liquor (PL) derived from apple tree branches, at different concentrations. The substrate removal, electrochemical properties, and microbial community characteristics were analysed to evaluate the performance of MFCs. Maximum current density (1.94 A/m2), coulombic efficiency (28%), and phenol removal rate (84%) were achieved with MFCs fed with PL at the optimal concentration of 1 g chemical oxygen demand (COD)/L. The polarisation test, cyclic voltammetry, and electrochemical impedance of the electrode redox reaction further explained how the addition of PL could stimulate formation of the electrochemically active biofilm, at the optimal concentration of 1 g COD/L. The microbial community of the anodic biofilm demonstrated that MFCs fed with 1 g COD/L had the highest relative abundance of the typical electrogenic bacteria Geobacter (33%), followed by Sphaerochaeta (6%) and Clostridium (4%). The results revealed that syntrophic interaction of these functional microorganisms contributed significantly to the PL degradation and electrical current generation.

    更新日期:2018-11-21
  • Applicability of AuNPs@N-GQDs nanocomposite in the modeling of the amplified electrochemical Ibuprofen aptasensing assay by monitoring of riboflavin
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-11-18
    Mahmoud Roushani, Faezeh Shahdost-fard

    Here, an ultrasensitive and low-cost electrochemical aptasensing assay is developed based on the applicability of a fabricated nanocomposite from nitrogen-doped graphene quantum dots (N-GQDs) and gold nanoparticles (AuNPs). A modified glassy carbon electrode (GCE) with the AuNPs@N-GQDs nanocomposite (AuNPs@N-GQDs/GCE) as an efficient platform has some unique properties such as high surface area and electrical conductivity. Furthermore, the prepared platform is capable of more loading of aptamer (Apt) molecules as a biological recognition element of Ibuprofen (IBP) on the modified electrode surface. It is noteworthy that in this study, riboflavin (RF) as a universal green probe is used for the first time for electrochemical detection of IBP. According to the proposed strategy and under the optimum condition, the unprecedented detection limit (LOD) of this assay (33.33 aM) is lower than previously reported analytical methods. The results demonstrate the ability of the nanocomposite for designing of the aptasensor, integrated within the electrode format, to cheaper and simpler detection of the IBP with a specificity and sensitivity sufficient for analysis in real samples. It seems that the proposed strategy based on the AuNPs@N-GQDs nanocomposite can be expanded to other nanomaterials. So, this is expected to have promising implications in the design of electrochemical sensors or biosensors for the detection of various targets.

    更新日期:2018-11-20
  • Electricity generation and microbial community in long-running microbial fuel cell for high-salinity mustard tuber wastewater treatment
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-11-16
    Linfang Zhang, Guokai Fu, Zhi Zhang

    High-salinity mustard tuber wastewater (MTWW) was utilized to obtain effluent treatment and recover bio-energy simultaneously in a microbial fuel cell (MFC). Since the long-term performance of MFCs in MTWW remains unclear, this study examined electricity generation and the microbial community that developed over a 195-day test. The MFC produced electricity over the entire testing period, with stable power output obtained on days 32–120. In the stable phase, a maximum power density of 12.43 W·m−3 was observed, and the internal resistance, open circuit voltage, and columbic efficiency (CE) were 148 Ω, 756 mV and 36.7 ± 1.2%, respectively. Chemical Oxygen Demand (COD) and Biochemical Oxygen Demand (BOD) removal continuously increased to 89.0 ± 1.5% and 98.6 ± 2.0%, respectively, the maximum rates that were obtained at the end of the experiment, respectively. In addition, 16S rRNA gene sequencing analysis showed that hydrolytic/fermentative bacteria could be considered as the bioanode core microbiome, constituting 36.90% of the microbiome. Sulfate-reducing bacteria (SRB), including Dethiosulfovibrio, Thermovirga, Desulfovibrio, and Desulfuromonas, eventually outcompeted the exoelectrogens completely, causing an irreversible loss in CE. This study provides more ideas for treatment and utilization of high-salinity MTWW.

    更新日期:2018-11-17
  • Electroautotrophy of Thioalkalivibrio nitratireducens
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-11-16
    Mickaël Rimboud, Wafa Achouak

    The electrochemical behavior and electro-autotrophy of a halo-alkaliphilic chemo-autotrophic Ectothiorhodospiraceae isolated from a soda lake, Thioalkalivibrio nitratireducens, were investigated using electrochemical methods and confocal fluorescence microscopy. The electrocatalysis of oxygen reduction was observed at −0.25 V/Ag/AgCl (−0.055 V/ SHE) with bioelectrodes polarized at −0.3 V/Ag/AgCl (−0.105 V/SHE), displaying a maximum catalytic current density of −620 mA m−2 (voltammetry). No catalytic signal toward nitrate reduction was observed under anaerobia. The microscopic observation of the polarized electrodes compared to non-polarized ones, however, revealed a bacterial proliferation both under aerobic and anaerobic conditions, demonstrating the ability of Tv. nitratireducens to grow with the polarized electrode as sole electron source.

    更新日期:2018-11-17
  • Interaction of prednisone with dsDNA at silver nanoparticles/poly(glyoxal-bis(2-hydroxyanil))/dsDNA modified electrode and its analytical application
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-11-16
    Gözde Aydoğdu Tığ, Derya Koyuncu Zeybek, Bülent Zeybek, Şule Pekyardımcı

    This paper reports the fabrication of an electrochemical DNA biosensor for the electrochemical determination of prednisone (PRD), which is a synthetic corticosteroid. For this purpose, silver nanoparticles (AgNPs) and a new polymer film poly(glyoxal-bis(2-hydroxyanil)) (P(GBHA)) were electrochemically deposited on a glassy carbon electrode (GCE), respectively. Then, an electrochemical DNA biosensor was prepared onto this electrode surface (GCE/AgNPs/P(GBHA)) by the immobilization of dsDNA using a chronoamperometry method. The proposed electrode was characterized by FESEM, XPS, and cyclic voltammetry (CV). The interaction between the PRD and dsDNA immobilized on the GCE/AgNPs/P(GBHA) electrode was investigated via a differential pulse voltammetry (DPV) method and UV–Vis spectrophotometry. The experimental factors affecting the interaction between the PRD concentration and dsDNA were optimized. The fabricated biosensor showed a wide linear response in a PRD concentration range of 1.0–50.0 μg mL−1 depending on both the adenine and guanine base signals. The detection limit based on the guanine and adenine signals was 0.3 μg mL−1 and 0.25 μg mL−1, respectively. The sensor exhibited excellent anti-interferential ability, good stability and reproducibility and was satisfactorily employed for the electrochemical assay of PRD in serum samples. The new DNA biosensor can be utilized for the sensitive, accurate and rapid analysis of PRD.

    更新日期:2018-11-17
  • Capacitively coupled electrical stimulation of rat chondroepiphysis explants: A histomorphometric analysis
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-11-08
    J.J. Vaca-González, J.F. Escobar, J.M. Guevara, Y.A. Hata, G. Gallego Ferrer, D.A. Garzón-Alvarado

    The growth plate is a cartilaginous layer present from the gestation period until the end of puberty where it ossifies joining diaphysis and epiphysis. During this period several endocrine, autocrine, and paracrine processes within the growth plate are carried out by chondrocytes; therefore, a disruption in cellular functions may lead to pathologies affecting bone development. It is known that electric fields impact the growth plate; however, parameters such as stimulation time and electric field intensity are not well documented. Accordingly, this study presents a histomorphometrical framework to assess the effect of electric fields on chondroepiphysis explants. Bones were stimulated with 3.5 and 7 mV/cm, and for each electric field two exposure times were tested for 30 days (30 min and 1 h). Results evidenced that electric fields increased the hypertrophic zones compared with controls. In addition, a stimulation of 3.5 mV/cm applied for 1 h preserved the columnar cell density and its orientation. Moreover, a pre-hypertrophy differentiation in the center of the chondroepiphysis was observed when explants were stimulated during 1 h with both electric fields. These findings allow the understanding of the effect of electrical stimulation over growth plate organization and how the stimulation modifies chondrocytes morphophysiology.

    更新日期:2018-11-09
  • Coalesced thermal and electrotransfer mediated delivery of plasmid DNA to the skin
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-10-23
    Anna Bulysheva, James Hornef, Chelsea Edelblute, Chunqi Jiang, Karl Schoenbach, Cathryn Lundberg, Muhammad Arif Malik, Richard Heller

    Efficient gene delivery and expression in the skin can be a promising minimally invasive technique for therapeutic clinical applications for immunotherapy, vaccinations, wound healing, cancer, and peripheral artery disease. One of the challenges for efficient gene electrotransfer (GET) to skin in vivo is confinement of expression to the epithelium. Another challenge involves tissue damage. Optimizing gene expression profiles, while minimizing tissue damage are necessary for therapeutic applications. Previously, we established that heating pretreatment to 43 °C enhances GET in vitro. We observed a similar trend in vivo, with an IR-pretreatment for skin heating prior to GET. Currently, we tested a range of GET conditions in vivo in guinea pigs with and without preheating the skin to ~43 °C. IR-laser heating and conduction heating were tested in conjunction with GET. In vivo electrotransfer to the skin by moderately elevating tissue temperature can lead to enhanced gene expression, as well as achieve gene transfer in epidermal, dermal, hypodermal and muscle tissue layers.

    更新日期:2018-10-23
  • Functionalized carbon nanotube adsorption interfaces for electron transfer studies of galactose oxidase
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-10-19
    Mulugeta B. Wayu, Michael J. Pannell, Najwa Labban, William S. Case, Julie A. Pollock, Michael C. Leopold
    更新日期:2018-10-22
  • Bioelectrochemical Nitrogen fixation (e-BNF): Electro-stimulation of enriched biofilm communities drives autotrophic nitrogen and carbon fixation
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-10-16
    Laura Rago, Sarah Zecchin, Federica Villa, Andrea Goglio, Anna Corsini, Lucia Cavalca, Andrea Schievano

    A new approach to microbial electrosynthesis is proposed, aimed at producing whole biomass from N2 and inorganic carbon, by electrostimulation of complex microbial communities. On a carbon-based conductor under constant polarization (−0.7 V vs SHE), an electroactive biofilm was enriched with autotrophic nitrogen fixing microorganims and led to biomass synthesis at higher amounts (up to 18 fold), as compared to controls kept at open circuit (OC). After 110 days, the electron transfer had increased by 30-fold, as compared to abiotic conditions. Metagenomics evidenced Nif genes associated with autotrophs (both Archaea and Bacteria) only in polarized biofilms, but not in OC. With this first proof of concept experiment, we propose to call this promising field ‘bioelectrochemical nitrogen fixation’ (e-BNF): a possible way to ‘power’ biological nitrogen fixation, organic carbon storage and soil fertility against desertification, and possibly a new tool to study the development of early prokaryotic life in extreme environments.

    更新日期:2018-10-17
  • 更新日期:2018-10-11
  • Label-free independent quantitation of viable and non-viable cells using a multivariable multi-resonant sensor
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-10-10
    Radislav A. Potyrailo, Jon Dieringer, Victoria Cotero, Yongjae Lee, Steve Go, Matthew Schulmerich, Gunnar Malmquist, Andreas Castan, Klaus Gebauer, Vincent Pizzi

    Biological cells are utilized for diverse biotechnological and bioengineering purposes ranging from the production of biopharmaceuticals, to cell therapy, “human-on-a-chip” drug and toxicology assays, and drug-resistance tests. In these and other applications, it is critical to quantify the levels of not only viable but also non-viable cells. While traditional off-line cell-staining methods are available for counting of non-viable cells, many applications cannot periodically remove cells for their off-line analysis because of the risk of contamination or workflow logistics. Here we show in-situ label-free quantitation of viable and non-viable cells with multivariable multi-resonant sensors. We used Chinese hamster ovary (CHO) cells in suspension culture in single-use bioreactors as a representative example. The resonant sensor design strategy permitted enhanced sensor sensitivity versus conventional non-resonant measurements and probed the spectral dispersion of viable and non-viable cells with multiple resonances. These capabilities of label-free in-situ analysis of cell viability can be attractive in diverse cell applications such as cell suspensions, adhered cells, and their 3D assemblages.

    更新日期:2018-10-11
  • Molecular and histological study on the effects of electrolytic electroporation on the liver
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-09-30
    Yanpeng Lv, Yanfang Zhang, Boris Rubinsky

    This study examined the temporal physiological and molecular events following the treatment of the liver with a tissue ablation modality that combined electroporation with electrolysis (E2). Rat liver was treated with an E2 waveform and the tissue examined, 1 h, 3 h, 6 h and 24 h with: H&E, Masson Trichrome, TUNEL stains and Western blot. H&E and TUNEL stains have shown that cell death began to be evident 3 h and hepatocyte regeneration was seen 24 h after treatment. H&E and Masson trichrome have shown that the extracellular matrix and the large lumens, appeared intact after E2. Western blot has shown the following molecular events after E2: cleaved caspase 3–downgraded at 1 h, upgraded at 24 h (apoptosis); cleaved Caspase 1 and cleaved GSDMD–upgraded at 6 h (pyroptosis), RIP3–upgraded at 1 h, MLKL–upgraded at 3 h (necroptosis). The mechanism of cell death was possible initiated by necroptosis pathway. Pyroptosis pathway was also activated. The observation that cell death from E2 was by programed necrosis and the details on the temporal molecular pathways, may have value for the recent attempt to combine electroporation mediated ablation with immunological treatment, by demonstrating that the cell death from E2 involves an inflammatory response and by providing data that could be used to design the optimal timing for the injection of immunological adjuvants.

    更新日期:2018-10-01
  • The efficiency of heart protection with HTK or HTK-N depending on the type of ischemia
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-09-20
    Michael Schaefer, Martha-Maria Gebhard, Wolfgang Gross

    We investigated isolated guinea pig hearts (n = 121) in an ischemia/ reperfusion model with the aim to compare the efficiency of the cardioplegic solution HTK with its novel replacement HTKN. Following consolidation with Tyrode's solution, ischemia started either immediately or after preceding cardioplegia with HTK, HTKN, or modified HTK enriched with Ca. Ischemia lasted either 80 min at 30 °C, or 360 min at 5 °C, or 81 min at 30 °C with intermittent cardioplegic perfusion. During ischemia we measured intracellular calcium (iCa++) and the time of gap junction uncoupling (t-in). During reperfusion we measured the reestablishment of cell coupling (t-ret), left ventricular developed pressure (LVDP), and heart rhythm (VC-RR). In 5 °C groups, iCa++ at t-in was significantly higher than before ischemia, and longest t-in, shortest t-ret, and best VC-RR were observed after HTK-protection. Of all 30 °C groups, the intermittent group with modified HTK showed shortest t-ret, best VC-RR, and the highest LVDP. At 5 °C, HTK groups had higher LVDP than HTK-N groups, but not at 30 °C. The data suggest that the higher calcium level in the HTK-N solution improves reperfusion after short ischemia at 30 °C but for long lasting ischemia at 5 °C it is beneficial to use the HTK solution.

    更新日期:2018-09-21
  • Effect of external resistance on the sensitivity of microbial fuel cell biosensor for detection of different types of pollutants
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-09-17
    Yue Yi, Beizhen Xie, Ting Zhao, Zhaoming Li, Devard Stom, Hong Liu
    更新日期:2018-09-18
  • Low-cost nanowired α-MnO2/C as an ORR catalyst in air-cathode microbial fuel cell
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-09-15
    Mir Reza Majidi, Fatemeh Shahbazi Farahani, Mirghasem Hosseini, Iraj Ahadzadeh

    In this work, low cost α-MnO2 nanowires and α-MnO2 nanowires supported on carbon Vulcan (α-MnO2/C) have been synthesized via a simple and facile hydrothermal method for application in microbial fuel cells. The prepared samples have been characterized by X-ray diffraction (XRD), Raman spectroscopy and field emission scanning electron microscopy (FE-SEM). Electrocatalytic activities of the samples have been evaluated by means of cyclic voltammetry (CV), linear sweep voltammetry (LSV) and electrochemical impedance spectroscopy (EIS) in a neutral phosphate buffer solution. EIS was performed at different potentials to gain further insight into the kinetic properties of α-MnO2/C. Both catalysts were used in air cathode microbial fuel cells to achieve power densities of 180 and 111 mWm−2 for α-MnO2/C and pristine α-MnO2 nanowires, respectively. α-MnO2/C functions as a good and economical alternative for Pt free catalysts in practical MFC applications, as shown by the findings of stability test and voltage generation cycles in long-term operation of MFC.

    更新日期:2018-09-17
  • A novel bioelectrochemical method for real-time nitrate monitoring
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-09-15
    Shi-gang Su, Hao-yi Cheng, Ting-ting Zhu, Hong-cheng Wang, Ai-jie Wang

    Nitrate is one of the most common pollutants in the water environment. A key factor for the effective control and removal of nitrate is the ability to accurately determine the nitrate concentration in groundwater and the secondary effluent of wastewater treatment plants. Here, a bioelectrochemical method for real-time detection of the nitrate was developed. In this work, a kinetic model was developed to describe the correlation between the nitrate concentration and the current. Standard addition experiments showed the relative error between indicator predictions and ion chromatographic values ranged from 3.14% to 9.74%. The monitoring results of secondary effluent showed that the system could give a good response at different nitrate concentrations. The average error of not >10.85% between the indicator predictions and ion chromatographic values was demonstrated. This study offers a new method for the development of sustainable bioelectrochemical system (BES)-based technology for the real-time detection of nitrate in groundwater and the secondary effluent.

    更新日期:2018-09-17
  • 更新日期:2018-09-07
  • Electrical signal propagation within and between tomato plants
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-08-07
    Alexander G. Volkov, Yuri B. Shtessel
    更新日期:2018-08-08
  • 更新日期:2018-07-30
  • Influence of cytochrome charge and potential on the cathodic current of electroactive artificial biofilms
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-07-22
    Stéphane Pinck, Mengjie Xu, Romain Clement, Elisabeth Lojou, Frédéric Jorand, Mathieu Etienne

    An electroactive artificial biofilm has been optimized for the cathodic reduction of fumarate by Shewanella oneidensis. The system is based on the self-assembly of multi-walled carbon nanotubes with bacterial cells in the presence of a c-type cytochrome. The aggregates are then deposited on an electrode to form the electroactive artificial biofilm. Six c-type cytochromes have been studied, from bovine heart or Desulfuromonas and Desulfuvibrio strains. The isoelectric point of the cytochrome controls the self-assembly process that occurs only with positively-charged cytochromes. The redox potential of the cytochrome is critical for electron transfer reactions with membrane cytochromes of the Mtr pathway. Optimal results have been obtained with c3 from Desulfovibrio vulgaris Hildenborough having an isoelectric point of 10.2 and redox potentials of the four hemes ranging between −290 and −375 mV vs. SHE. A current density of 170 μA cm−2 could be achieved in the presence of 50 mM fumarate. The stability of the electrochemical response was evaluated, showing a regular decrease of the current within 13 h, possibly due to the inactivation or leaching of loosely-bound cytochromes from the biofilm.

    更新日期:2018-07-23
  • Direct electron transfer of lytic polysaccharide monooxygenases (LPMOs) and determination of their formal potentials by large amplitude Fourier transform alternating current cyclic voltammetry
    Bioelectrochemistry (IF 3.789) Pub Date : 2018-07-20
    D. Zouraris, M. Dimarogona, A. Karnaouri, E. Topakas, A. Karantonis

    MtLPMO9 and FoLPMO9 are two lytic polysaccharide monooxygenases (LPMOs), from the filamentous fungi Thermothelomyces thermophila and Fusarium oxysporum, respectively. In the present study an attempt has been made to achieve direct electron transfer between these enzymes and a glassy carbon electrode by immobilization in Nafion polyelectrolyte. The method used to ascertain the feasibility of direct electron transfer was large amplitude Fourier transform alternating current voltammetry (FTacV) and the formal potentials of these enzymes were determined at different temperatures. The findings of this paper indicate that LPMOs can be studied by direct electron transfer, which could be exploited in the near future for their biochemical characterization.

    更新日期:2018-07-21
Some contents have been Reproduced with permission of the American Chemical Society.
Some contents have been Reproduced by permission of The Royal Society of Chemistry.
导出
化学 • 材料 期刊列表