2025年

52. P. F. Liu^#, S. Li^#, J. Y. Zeng^#, M. X. Wang, J. L. Du, H. H. Wang, E. H. Xiong*, R. H. Yang*. Creating a Modular Activatable CRISPR-Cas12a System by Engineering crRNA Scaffold with a Steric Hindrance Effector. J. Am. Chem. Soc. 2025, 147, 31349–31364. (Link)

51. T. Y. Yuan, J. L. Du, J. J. Hu, J. M. Luo, X. X. Pu*, X. Y. Zhu, B. J. Chen, Y. L. Teng, H. Li, S. Li, L. Jiang*, E. H. Xiong*. CRISPR-Cas13a-based dual-channel AND-logic gated biosensor for the simultaneous assay of APE1 and miRNA-224. Chem. Commun. 2025, 61, 11673–11676. (Link)

50. Z. Jiang, S. L. Pan, J. H. Chen, H. H. Yi, Y. F. Li, Y. Qing, E. H. Xiong*, Z. Zou*. Lung-targeted feedback regulation of the mitochondrial ATP synthesis pathway for orthotopic tumor suppression. J. Mater. Chem. B 2025, 13, 9442–9451. (Link)

49. J. L. Du^#, H. L. Xu^#, X. Y. Zhu, K. Y. Long, J. Q. Lang, L. Jiang, E. H. Xiong*, J. W. Liu, R. H. Yang. Robust Peptide-Functionalized Gold Nanoparticles via Ethynyl Bonding for High-Fidelity Bioanalytical Applications. Angew. Chem. Int. Ed. 2025, 64, e202424351. (Link)

48. J. L. Ai^#, J. H. Deng^#, J. J. Hu, X. X. Pu*, T. Y. Yuan, Y. L. Teng, H. Li, B. J. Chen, J. L. Du, L. Jiang*, X. Y. Chen, E. H. Xiong*, R. H. Yang. PAM-Independent CRISPR-Cas12a System for Specific Assays of Single Nucleotide Variants. JACS Au 2025, 5, 1392–1401. (Link)

47. J. Y. Zeng^#, P. F. Liu^#, J. L. Du, S. Li, E. H. Xiong*, R. H. Yang. Split crRNA-Motivated Amplification-Free RNA Testing with CRISPR-Cas12a. Sci. China Chem. 2025, 68, 789–801. (Link)

46. Y. Su, Y. T. Liu, Y. L. Zhong, S. M. Ma, Q. Q. Ouyang, X. Chen, Y. F. Li, E. H. Xiong, Z. Zou*, J. W. Liu*. Coordination-driven self-assembly of biomolecules and metal ions: Advances in methodology and applications. Coordin. Chem. Rev. 2025, 527, 216403. (Link)

2024年

45. P. F. Liu^#, J. Y. Zeng^#, C. C. Jiang^#, J. L. Du, L. Jiang*, S. Li, F. X. Zeng*, E. H. Xiong*. Poly(vinylpyrrolidone)-Enhanced CRISPR-Cas System for Robust Nucleic Acid Diagnostics. Anal. Chem. 2024, 96, 15797–15807. (Link)

44. P. F. Liu^#, Y. T. Lin^#, X. H. Zhuo^#, J. Y. Zeng, B. L. Chen, Z. Zou, G. H. Liu, E. H. Xiong*, R. H. Yang*. Universal crRNA Acylation Strategy for Robust Photo-Initiated One-Pot CRISPR-Cas12a Nucleic Acid Diagnostics. Angew. Chem. Int. Ed. 2024, 63, e202401486. (Link)

43. E. H. Xiong^#, P. F. Liu^#, R. J. Deng, K. X. Zhang, R. H. Yang*, J. H. Li*. Recent Advances in Enzyme-Free and Enzyme-Mediated Single-Nucleotide Variation Assay in Vitro. Natl. Sci. Rev. 2024, 11, nwae118. (Link)

42. Z. Zou*, S. L. Pan, Q. Xue, T. Chen, Z. Y. Huang, B. Qing, P. F. Liu, C. H. Zhao, Y. L. Sun, E. H. Xiong*, R. H. Yang. Artificial Nucleic Acid Tractor-Directed Simultaneous Depletion of Oncogenic Membrane Proteins without Hijacking Proteolysis-Specific Actuator. CCS Chem. 2024, 6, 439–449. (Link)

41. L. Jiang, J. L. Du, H. L. Xu, X. H. Zhuo, J. L. Ai, J. Y. Zeng, R. H. Yang, E. H. Xiong*. Ultrasensitive CRISPR/Cas13a-Mediated Photoelectrochemical Biosensors for Specific and Direct Assay of miRNA-21. Anal. Chem. 2023, 95, 1193–1200. (Link)

 

Researches at SCNU (2019-2022)

40. M. Q. Huang, E. H. Xiong*, Y. Wang, M. L. Hu, H. H. Yue, T. Tian, D. B. Zhu, H. Liu, X. M. Zhou*. Fast Microwave Heating-Based One-Step Synthesis of DNA and RNA Modified Gold Nanoparticles. Nat. Commun. 2022, 13, 968.

39. M. Lin, H. H. Yue, T. Tian, E. H. Xiong, D. B. Zhu, Y. Z. Jiang*, X. M. Zhou*. Glycerol Additive Boosts 100-fold Sensitivity Enhancement for One-Pot RPA-CRISPR/Cas12a Assay. Anal. Chem. 2022, 94, 8277–8284.

38. Z. R. Bi, M. L. Hu, Y. Z. Jiang, E. H. Xiong, B. W. Shu, S. Q. Li, H. W. Chen*, X. H. Chen*, X. M. Zhou*. Development of a Handheld Nano‑centrifugal Device for Visual Virus Detection. J. Anal. Test. 2022, 6, 353–364.

37. S. S. Wang^#, E. H. Xiong^#, S. Bhadra, A. D. Ellington*. Developing predictive hybridization models for phosphorothioate oligonucleotides using high-resolution melting. PLoS One 2022, DOI: 10.1371/journal.pone.0268575.

36. H. H. Yue, B. W. Shu*, T. Tian, E. H. Xiong, M. Q. Huang, D. B. Zhu, J. Sun, Q. Liu, S. C. Wang, Y. R. Li, X. M. Zhou*. Droplet Cas12a Assay Enables DNA Quantification from Unamplified Samples at the Single-Molecule Level. Nano Lett. 2021, 21, 4643–4653.

35. E. H. Xiong, L. Jiang, T. Tian, M. L. Hu, H. H. Yue, M. Q. Huang, W. Lin, Y. Z. Jiang, D. B. Zhu, X. M. Zhou*. Simultaneous Dual-Gene Diagnosis of SARS-CoV-2 Based on CRISPR/Cas9-Mediated Lateral Flow Assay. Angew. Chem. Int. Ed. 2021, 60, 5307–5315.

34. E. H. Xiong, D. B. Yao, A. D. Ellington*, S. Bhadra*. Minimizing Leakage in Stacked Strand Exchange Amplification Circuits. ACS Synth. Biol. 2021, 10, 1277–1283.

33. H. H. Yue, M. Q. Huang, T. Tian, E. H. Xiong, X. M. Zhou*. Advances in Clustered, Regularly Interspaced Short Palindromic Repeats (CRISPR)-Based Diagnostic Assays Assisted by Micro/Nanotechnologies. ACS Nano 2021, 15, 7848–7859.

32. W. Lin, T. Tian, Y. Z. Jiang, E. H. Xiong, D. B. Zhu, X. M. Zhou*. A CRISPR/Cas9 eraser strategy for contamination-free PCR end-point detection. Biotechnol. Bioeng. 2021, 118, 2053–2066.

31. M. Cheng, E. H. Xiong, T. Tian, D. B. Zhu, H. Q. Ju, X. M. Zhou*. A CRISPR-driven colorimetric code platform for highly accurate telomerase activity assay. Biosens. Bioelectron. 2021, 172, 112749.

30. M. L. Hu, C. Q. Yuan, T. Tian, X. S. Wang, J. Sun, E. H. Xiong*, X. M. Zhou*. Single-Step, Salt-Aging-Free, and Thiol-Free Freezing Construction of AuNP-Based Bioprobes for Advancing CRISPR-Based Diagnostics. J. Am. Chem. Soc. 2020, 142, 7506–7513.

29. X. S. Wang, E. H. Xiong*, T. Tian, M. Cheng, W. Lin, H. Wang, G. H. Zhang, J. Sun*, X. M. Zhou* Clustered Regularly Interspaced Short Palindromic Repeats/Cas9-Mediated Lateral Flow Nucleic Acid Assay. ACS Nano 2020, 14, 2497–2508.

28. Y. J. Bao, Y. Z. Jiang, E. H. Xiong*, T. Tian, Z. Z. Zhang, J. Lv, Y. Li*, X. M. Zhou*. CUT-LAMP: Contamination-Free Loop-Mediated Isothermal Amplification Based on the CRISPR/Cas9 Cleavage. ACS Sens. 2020, 5, 1082–1091.

27. D. S. Zhen, X. H. Luo, D. Yang, H. T. Zou, E. H. Xiong*, Y. Liu*. Nd@TiO₂ Nanocomposite for Photocatalytic Inactivation of Escherichia coli. J. Nanosci. Nanotechnol. 2020, 20, 1447–1453.

26. D. B. Yao, S. Bhadra, E. H. Xiong, H. J. Liang, A. D. Ellington*, C. Jung*. Dynamic Programming of a DNA Walker Controlled by Protons. ACS Nano 2020, 14, 4007–4013.

25. Q. Chen, T. Tian, E. H. Xiong, P. Wang*, X. M. Zhou*. CRISPR/Cas13a Signal Amplification Linked Immunosorbent Assay for Femtomolar Protein Detection. Anal. Chem. 2020, 92, 573–577.

24. C. Q. Yuan^#, T. Tian^#, J. Sun^#, M. L. Meng, X. S. Wang, E. H. Xiong, M. Cheng, Y. J. Bao, W. Lin, et al. Universal and Naked-Eye Gene Detection Platform Based on the Clustered Regularly Interspaced Short Palindromic Repeats/Cas12a/ 13a System. Anal. Chem. 2020, 92, 4029–4037.

23. E. H. Xiong, D. S. Zhen, L. Jiang*, X. M. Zhou*. Binding-Induced 3D-Bipedal DNA Walker for Cascade Signal Amplification Detection of Thrombin Combined with Catalytic Hairpin Assembly Strategy. Anal. Chem. 2019, 91, 15317–15324.

 

M.S. and Ph.D. Research (2012-2018)

22. E. H. Xiong, Deshuai Zhen, Ling Jiang*. Cascade signal amplified assay of nucleic acids based on entropy-driven amplification strategy and Mg²⁺-dependent DNAzyme cleavage. Talanta 2019, 198, 179–184.

21. E. H. Xiong, L. Jiang*. An ultrasensitive electrochemical immunoassay based on a proximity hybridization-triggered three-layer cascade signal amplification strategy. Analyst 2019, 144, 634–640.

20. E. H. Xiong, D. S. Zhen, L. Jiang*. Homogeneous enzyme-free and entropy-driven isothermal fluorescent assay for nucleic acids based on a dual-signal output amplification strategy. Chem. Commum. 2018, 54, 12594–12597.

19. E. H. Xiong, X. X. Yan, X. H. Zhang, Y. M. Li, R. Y. Yang, L. X. Meng, J. H. Chen*. A new photoelectrochemical biosensor for ultrasensitive determination of nucleic acids based on a three-stage cascade signal amplification strategy. Analyst 2018, 143, 2799–2806.

18. E. H. Xiong, D. S. Zhen, L. Jiang*. Homogeneous Enzyme-Free and Entropy-Driven Isothermal Fluorescent Assay for Nucleic Acids Based on a Dual-Signal Output Amplification Strategy. Chem. Commun. 2018, 54, 12594–12597.

17. E. H. Xiong, X. X. Yan, X. H. Zhang*, Y. Q. Liu, J. W. Zhou, J. H. Chen*. Exonuclease III–Assisted Cascade Signal Amplification Strategy for Label-Free and Ultrasensitive Electrochemical Detection of Nucleic Acids. Biosens. Bioelectron. 2017, 87, 732–736.

16. Y. Q. Liu, E. H. Xiong, X. Y. Li, J. J. Li, X. H. Zhang*, J. H. Chen*. Sensitive electrochemical assay of alkaline phosphatase activity based on TdT-mediated hemin/G-quadruplex DNAzyme nanowires for signal amplification. Biosens. Bioelectron. 2017, 87, 970–975.

15. E. H. Xiong, Z. Z. Li, X. H. Zhang*, J. W. Zhou, X. X. Yan, Y. Q. Liu, J. H. Chen*. Triple-Helix Molecular Switch Electrochemical Ratiometric Biosensor for Ultrasensitive Detection of Nucleic Acids. Anal. Chem. 2017, 89, 8830–8835.

14. P. Yu, X. H. Zhang*, E. H. Xiong, J. W. Zhou, X. Y. Li, J. H. Chen*. A label-free and cascaded dual-signaling amplified electrochemical aptasensing platform for sensitive prion assay. Biosens. Bioelectron. 2016, 85, 471–478.

13. P. Yu, Y. Q. Liu, X. H. Zhang*, J. W. Zhou, E. H. Xiong, X. Y. Li, J. H. Chen*. A novel electrochemical aptasensor for bisphenol A assay based on triple-signaling strategy. Biosens. Bioelectron. 2016, 79, 22–28.

12. E. H. Xiong, X. H. Zhang*, Y. Q. Liu, J. W. Zhou, P. Yu, J. H. Chen*. An electrochemical biosensor for sensitive detection of Hg2+ based on exonuclease III-assisted target recycling and hybridization chain reaction amplification strategies. Anal. Methods 2016, 8, 2106–2111.

11. J. W. Zhou, X. H. Zhang, E. H. Xiong, P. Yu, X. Y. Li, J. H. Chen*. SDR-recycling signal amplification for highly sensitive methyltransferase activity assay. J. Electroanal. Chem. 2016, 781, 304–309.

10. Y. Q. Liu, X. Zhang, J. S. Yang, E. H. Xiong, X. H. Zhang, J. H. Chen*. Sensitive detection of bisphenol A based on a ratiometric electrochemical aptasensor. Can. J. Chem. 2016, 94, 509–514.

9. E. H. Xiong, X. H. Zhang, Y. Q. Liu, J. W. Zhou, P. Yu, X. Y. Li, J. H. Chen*. Ultrasensitive Electrochemical Detection of Nucleic Acids Based on the Dual-Signaling Electrochemical Ratiometric Method and Exonuclease III-Assisted Target Recycling Amplification Strategy. Anal. Chem. 2015, 87, 7291–7296.

8. E. H. Xiong, L. Wu, J. W. Zhou, P. Yu, X. H. Zhang*, J. H. Chen*. A Ratiometric Electrochemical Biosensor for Sensitive Detection of Hg²⁺ Based on Thymine-Hg²⁺-Thymine Structure. Anal. Chim. Acta 2015, 853, 242–248.

7. L. Wu, E. H. Xiong, Y. Yao, X. Zhang, X. H. Zhang*, J. H. Chen*. A new electrochemical aptasensor based on electrocatalytic property of graphene toward ascorbic acid oxidation. Talanta 2015, 134, 699–704.

6. P. Yu, X. H. Zhang, J. W. Zhou, E. H. Xiong, X. Y. Li, J. H. Chen*. Smart protein biogate as a mediator to regulate competitive host-guest interaction for sensitive ratiometric electrochemical assay of prion. Sci. Rep. 2015, 5, 16015.

5. J. W. Zhou, X. H. Zhang, E. H. Xiong, P. Yu, J. H. Chen*. A label-free electrochemical strategy for highly sensitive methyltransferase activity assays. Chem. Commun. 2015, 51, 5081–5084.

4. J. W. Zhou, W. Y. Wang, P. Yu, E. H. Xiong, X. H. Zhang, J. H. Chen*. A simple label-free electrochemical aptasensor for dopamine detection. RSC Adv. 2014, 4, 52250–52255.

3. P. Yu, J. W. Zhou, L. Wu, E. H. Xiong, X. H. Zhang*, J. H. Chen*. A ratiometric electrochemical aptasensor for sensitive detection of protein based on aptamer–target–aptamer sandwich structure. J. Electroanal. Chem. 2014, 732, 61–65.

2. L. Wu, E. H. Xiong, X. Zhang, X. H. Zhang, J. H. Chen*. Nanomaterials as signal amplification elements in DNA-based electrochemical sensing. Nano Today 2014, 9, 197–211.

1. L. Wu, X. H. Zhang, W. Liu, E. H. Xiong, J. H. Chen*. Sensitive Electrochemical Aptasensor by Coupling "Signal-on" and "Signal-off" Strategies. Anal. Chem. 2013, 85, 8397–8402.