Journal Paper
2024
1. Wenshuai Wu; Ying Mu; Microfluidic technologies for advanced antimicrobial susceptibility testing, Biomicrofluidics, 2024, 18(3), 031504. https://doi.org/10.1063/5.0190112
2. Wenshuai Wu; Qianbin Zhao; Boran Zhang; Viable Escherichia coli enumeration on a polydimethylsiloxane (PDMS) chip with vertical channel-well configuration, Microchim. Acta, 2024,191 (5), 241. https://www.researchgate.net/publication/379570069
3. Wenshuai Wu; Simon Chun Kiat Goh; Gaozhe Cai; Shilun Feng; Boran Zhang; Digital metabolic activity assay enables fast assessment of 2D materials bactericidal efficiency, Analytica Chimica Acta, 2024, 1285: 342007. https://www.researchgate.net/publication/375511698
4. Haoqiang Feng; Shitao Shen; Mingliang Jin; Mengjie Xiao; Mengjun Liu; Qilin Zhang; Hongwei Jiang, Zichuan Yi; Wenshuai Wu; Guofu Zhou; Lingling Shui; Massive Electro‐Microfluidic Particle Assembly Patterns in Droplet Array for Information Encoding, Small, 2024, 2405161. https://www.researchgate.net/publication/383822045
2023
1. Wenshuai Wu; Boran Zhang; Weihong Yin; Liping Xia; Yuanjie Suo; Gaozhe Cai; Yang Liu; Wei Jin; Qianbin Zhao; Ying Mu; Enzymatic Antimicrobial Susceptibility Testing with Bacteria Identification in 30 min, Analytical Chemistry, 2023, 95(44): 16426-16432. https://www.researchgate.net/publication/374954533
2. Wenshuai Wu; Gaozhe Cai; Yang Liu; Yuanjie Suo; Boran Zhang; Wei Jin; Yinghua Yu; Ying Mu ; Direct single-cell antimicrobial susceptibility testing of Escherichia coli in urine using a ready-to-use 3D microwell array chip, Lab on a chip, 2023, 23(10), 2399-2410. https://www.researchgate.net/publication/368521631
3. Wenshuai Wu; Yuanjie Suo; Qianbin Zhao; Gaozhe Cai; Yang Liu; Wei Jin; Ying Mu; Boran Zhang; Inoculum size-insensitive susceptibility determination of urine sample based on in-situ measurement of inducible enzyme activity after 20 min of antibiotic exposure, Analytica Chimica Acta, 2023, 1282: 341858. https://www.researchgate.net/publication/374843455
4. Boran zhang; Wenhan Zhao; Dan Yuan; Tiao Wang; Wenshuai Wu*；A novel microparticle size sorting technology based on sheath flow stable expansion regimes, Physics of fluidics, 2023, 35: 052018. ( 通讯作者 ) https://doi.org/10.1063/5.0152581
5. Boran Zhang; Wenhan Zhao; Ziyu Wang; Qianbin Zhao; Wenshuai Wu*; Assistance of inertial microfluidics in membrane-based microfiltration, Journal of Water Process Engineering, 2023, 53: 103762 . ( 通讯作者 ) https://doi.org/10.1016/j.jwpe.2023.103762
6. Boran Zhang; Wenshuai Wu; Qianbin Zhao; Sheng Yan ; Geometric optimization of double layered microchannel with grooves array for enabling nanoparticle manipulation, Physics of Fluids, 2023, 35: 062009. https://doi.org/10.1063/5.0152929
7. Wenhan Zhao; Xiaopeng Shang; Boran Zhang; Dan Yuan; Binh Thi Thanh Nguye; Wenshuai Wu; Jing Bo Zhang; Niancai Peng; Ai Qun Liu; Fei Duan; Lip Ket Chin ; Squeezed state in the hydrodynamic focusing regime for Escherichia coli bacteria detection, Lab on a Chip, 2023, 23: 5039-5046. https://www.researchgate.net/publication/375217920
8. Boran Zhang; Wenhan Zhao; Wenshuai Wu; Xiaopeng Shang; Binh Thi Thanh Nguyen; Jing Bo Zhang; Eric Peng Huat Yap; Ai Qun Liu; Lip Ket Chin ; Dean Vortex Trapping for Concentrating Escherichia coli in a Cross-Flow Microfilter, ACS Applied Engineering Materials, 2023, 1(10): 2809-2816. https://pubs.acs.org/doi/10.1021/acsaenm.3c00502
2022
1. Wenshuai Wu; Binh Thi Thanh Nguyen; Patricia Yang Liu; Gaozhe Cai; Shilun Feng; Yuzhi Shi; Boran Zhang; Yuzhi Hong; Ruozhen Yu; Xiaohong Zhou; Ai Qun Liu; Yi Zhang; Eric Peng Huat Yap; Lip Ket Chin; Single Escherichia coli bacteria detection using a chemiluminescence digital microwell array chip., Biosensors and Bioelectronics, 2022, 215: 114594-114594. https://www.researchgate.net/publication/362376489
2. Wenshuai Wu; Binh Thi Thanh Nguyen; Patricia Yang Liu; Gaozhe Cai; Shilun Feng; Yuzhi Shi; Boran Zhang; Yuzhi Hong; Ruozhen Yu; Xiaohong Zhou; Yi Zhang; Eric Peng Huat Yap; Ai Qun Liu; Lip Ket Chin; A self-driven carbon-doped high-density microwell array for single cell analysis, Sensors and Actuators B-chemical, 2022, 368: 132198-132198. https://www.researchgate.net/publication/361384562
3. Wenshuai Wu; Qianbin Zhao; Gaozhe Cai; Boran Zhang; Yuanjie Suo; Yang Liu; Wei Jin; Ying Mu; All-In-One Escherichia coli Viability Assay for Multi-dimensional Detection of Uncomplicated Urinary Tract Infections, Analytical Chemistry, 2022, 94(51): 17853-17860. https://www.researchgate.net/publication/366361002
4. Boran Zhang; Fan Yang; Wenshuai Wu*; Wuyi Wan; Wenhan Zhao; Qianbin Zhao; Investigation of particle manipulation mechanism and size sorting strategy in a double-layered microchannel., Lab on a Chip, 2022, 22(23): 4556-4573. ( 通讯作者 ) https://doi.org/10.1039/D2LC00822J
5. Boran Zhang; Siyuan Zou; Wenshuai Wu*; Fan Yang; Kashif Mehmood; Wuyi Wan; Qianbin Zhao ; Mechanism and Effects of Cellular Creep in a Microfluidic Filter, Journal of Physical Chemistry Letters, 2022, 13: 8641-8647. ( 通讯作者 ) https://pubs.acs.org/doi/10.1021/acs.jpclett.2c02137
6. Wenhan Zhao; Wenshuai Wu#; Dan Yuan; Siyuan Zou; Fan Yang; Qianbin Zhao; Kashif Mehmood; Boran Zhang; Experimental exploration on stable expansion phenomenon of sheath flow in viscous microfluidics, Physics of Fluids, 2022, 34(12): 122002.( 共同一作 ) https://doi.org/10.1063/5.0129764
7. Simon Chun Kiat Goh; Wenshuai Wu#; Chun Fei Siah; Derek Keng Yang Phee; Aiqun Liu; Beng Kang Tay ; Surface disinfection with silver loaded pencil graphite prepared with green UV photoreduction technique, Nanotechnology, 2022, 33(23): 235602.（ 共同一作 ) https://www.researchgate.net/publication/358601726
8. Yuanjie Suo; Weihong Yin; Wenshuai Wu; Wenjian Cao; Qiangyuan Zhu; Ying Mu ; A large-scale pico-droplet array for viable bacteria digital counting and dynamic tracking based on a thermosetting oil, Analyst, 2022, 147: 3305-3314. https://doi.org/10.1039/D2AN00680D
9. Yuanjie Suo; Weihong Yin; Qiangyuan Zhu; Wenshuai Wu; Wenjian Cao; Ying Mu ; A Specific and Sensitive Aptamer-Based Digital PCR Chip for Salmonella typhimurium Detection, Biosensors, 2022, 12(7): 458-458. https://www.researchgate.net/publication/362314479
2021
1. Wenshuai Wu; Shan Zhang; Tao Zhang; Ying Mu; Immobilized Droplet Arrays in Thermosetting Oil for Dynamic Proteolytic Assays of Single Cells, ACS Applied Materials & Interfaces, 2021, 13(5): 6081-6090. https://www.researchgate.net/publication/348839364
2. Gaozhe Cai; Wenshuai Wu; Shilun Feng; Yuanjie Liu ; Label-free E. coli detection based on enzyme assay and a microfluidic slipchip, The Analyst, 2021, 146(14): 4622-4629. https://www.researchgate.net/publication/352204950
3. Yuzhi Shi; Zhenyu Li; Patricia Yang Liu; Binh Thi Thanh Nguyen; Wenshuai Wu; Qianbin Zhao; Lip Ket Chin; Minggui Wei; Peng Huat Yap; Xiaohong Zhou; Hongwei Zhao; Dan Yu; Din Ping Tsai; Ai Qun Liu ; On-Chip Optical Detection of Viruses: A Review, Advanced Photonics Research, 2021, 2: 2000150. https://www.researchgate.net/publication/348515657
2020
1. Wenshuai Wu; Fengtian Wu; Shan Zhang; Xiong Ding; Tao Zhang; Ying Yang; Ying Mu; A self-powered bidirectional partition microfluidic chip with embedded microwells for highly sensitive detection of EGFR mutations in plasma of non-small cell lung cancer patients, Talanta, 2020, 220: 121426. https://www.researchgate.net/publication/343117588
2. Yu He; Juxin Yin; Wenshuai Wu; Hongxiao Liang; Futianchun Zhu; Ying Mu; Hongliang Fan; Tao Zhang ; Rapid In Situ Photoimmobilization of a Planar Droplet Array for Digital PCR, Analytical Chemistry, 2020, 92(12): 8530-8535. https://pubs.acs.org/doi/10.1021/acs.analchem.0c01304
2019
1. Tong Gou; Jiumei Hu; Shufang Zhou; Wenshuai Wu; Weibo Fang; Jingjing Sun; Zhenming Hu; Haotian Shen; Ying Mu ; A new method using machine learning for automated image analysis applied to chip-based digital assays, The Analyst, 2019, 144(10): 3274-3281. https://doi.org/10.1039/C9AN00149B
2. Zhenming Hu; Weibo Fang; Tong Gou; Wenshuai Wu; Jiumei Hu; Shufang Zhou; Ying Mu ; A novel method based on Mask R-CNN model for processing dPCR images, Analytical Methods, 2019, 11: 3410-3418. https://doi.org/10.1039/C9AY01005J
3. Jingjing Sun; Jiumei Hu; Tong Gou; Xiong Ding; Qi Song; Wenshuai Wu; Guoping Wang; Juxin Yin; Ying Mu ; Power-free polydimethylsiloxane femtoliter-sized arrays for bead-based digital immunoassays, Biosensors and Bioelectronics, 2019, 139: 111339. https://doi.org/10.1016/j.bios.2019.111339
4. Jiumei Hu; Tong Gou; Wenshuai Wu; Jingjing Sun; Shan Zhang; Shufang Zhou; Juxin Yin; Ying Mu ; Proximity ligation assays for precise quantification of femtomolar proteins in single cells using self-priming microfluidic dPCR chip, Analytica Chimica Acta, 2019, 1076: 118-124. https://doi.org/10.1016/j.aca.2019.05.034
5. Shufang Zhou; Tong Gou; Jiumei Hu; Wenshuai Wu; Xiong Ding; Weibo Fang; Zhenming Hu; Ying Mu ; A highly integrated real-time digital PCR device for accurate DNA quantitative analysis, Biosensors and Bioelectronics, 2019, 128: 151-158. https://doi.org/10.1016/j.bios.2018.12.055
2018
1. Wu, Wenshuai; Zhou, Shufang; Hu, Jiumei; Wang, Guoping; Ding, Xiong; Gou, Tong; Sun, Jingjing; Zhang, Tao; Mu, Ying; A Thermosetting Oil for Droplet-Based Real-Time Monitoring of Digital PCR and Cell Culture, ADVANCED FUNCTIONAL MATERIALS, 2018, 39(28): 1803559. https://www.researchgate.net/publication/326871839
2. Tong Gou; Jiumei Hu; Wenshuai Wu; Xiong Ding; Shufang Zhou; Weibo Fang; Ying Mu ; Smartphone-based mobile digital PCR device for DNA quantitative analysis with high accuracy, Biosensors and Bioelectronics, 2018, 120: 144-152 . https://doi.org/10.1016/j.bios.2018.08.030
3. Pang, Bo; Fu, Kaiyue; Liu, Yushen; Ding, Xiong; Hu, Jiumei; Wu, Wenshuai; Xu, Kun; Song, Xiuling; Wang, Juan; Mu, Ying; Zhao, Chao; Li, Juan; Development of a self-priming PDMS/paper hybrid microfluidic chip using mixed-dye-loaded loop-mediated isothermal amplification assay for multiplex foodborne pathogens detection, ANALYTICA CHIMICA ACTA, 2018, 1040(1040): 81-89. https://doi.org/10.1016/j.aca.2018.07.024
2017
1. 吴文帅; 丁雄; 牟颖; 负压驱动皮升级等温核酸精确定量微流控芯片, 色谱, 2017, 35(3): 351-356.
2. Pang, Bo; Ding, Xiong; Wang, Guoping; Zhao, Chao; Xu, Yanan; Fu, Kaiyue; Sun, Jingjing; Song, Xiuling; Wu, Wenshuai; Liu, Yushen; Song, Qi; Hu, Jiumei; Li, Juan; Mu, Ying ; Rapid and Quantitative Detection of Vibrio parahemolyticus by the Mixed-Dye-Based Loop-Mediated Isothermal Amplification Assay on a Self-Priming Compartmentalization Microfluidic Chip, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2017, 51(65): 11312-11319. https://pubs.acs.org/doi/10.1021/acs.jafc.7b03655
3. Wang, Guoping, Ding, Xiong, Hu, Jiumei, Wu, Wenshuai, Sun, Jing, Mu, Ying. Unusual isothermal multimerization and amplification by the strand-displacing DNA polymerases with reverse transcription activities. Scientific reports, 2017, 7(1), 1-10. https://www.researchgate.net/publication/320593584
2015
1. Ding, Xiong; Wu, Wenshuai; Zhu, Qiangyuan; Zhang, Tao; Jin, Wei; Mu, Ying; Mixed-Dye-Based Label-Free and Sensitive Dual Fluorescence for the Product Detection of Nucleic Acid Isothermal Multiple-Self-Matching-Initiated Amplification, Analytical Chemistry, 2015, 20(87): 10306-10314 .（ 共同一作 ) https://www.researchgate.net/publication/282042474
Conference Paper
1. Y. Su, Z. Lai, Z. Zhang, S. Feng, W. Wu*, G. Cai*, J. Zhao. ESCHERICHIA COLI QUANTITATIVE RECOGNITION SYSTEM BASED ON IMAGE PROCESSING AND DEEP LEARNING, MicroTAS, 2025.
2. Zhang, J. B., Wu, W. S., Soh, W. M., Natesan, P. B., Yap, E. P. H., Zhang, B., ... & Liu, A. Q. (2023, May). Online Inspection of Microbial with an Opto-Microfluidic Chip. In CLEO: Applications and Technology (pp. ATh3I-4). Optica Publishing Group.
3. Cai, G., Feng, S., Liu, Y., Wu, W., Zhang, J., Lin, J., & Liu, A. Q. (2020, October). Design and microfabrication of microreactor array for plate reader for E. coli research. In 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 (pp. 434-435). Chemical and Biological Microsystems Society.
4. 吴文帅, 张珊, 朱强远, &牟颖. (2019). 微流控数字PCR 用于循环肿瘤DNA 高灵敏检测. 中国化学会第十二届全国微全分析系统学术会议、第七届全国微纳尺度生物分离分析学术会议、第七届国际微流控学学术论坛, ,1-1.
5. 张珊, 吴文帅, &牟颖. (2019). 基于静态液滴阵列的单个Jurkat细胞分泌的高通量分析. 中国化学会第十二届全国微全分析系统学术会议、第七届全国微纳尺度生物分离分析学术会议、第七届国际微流控学学术论坛, ,1-1.
Patents
1. 牟颖, 吴文帅，金伟，一种可凝固的油相混合物及其应用, 中国发明专利，专利号：CN 108543504 B；（授权）
2. 牟颖, 吴文帅，金伟，一种通道内壁涂覆Parylene的微流控芯片制作方法, 中国发明专利，专利号：CN 108545692 B；（授权）
3. 张博然, 吴文帅, 方越, 刘琳, 冉启华，一种基于PDMS微通道的被动式双体积液滴同步生成与收集芯片、装置及方法，中国发明专利，专利号：CN 118341498 B；（授权）
4. 牟颖, 吴文帅，一种快速的病原菌特异性酶促药敏分析方法, 中国发明专利，受理号：2023112474281；（审查）
5. 牟颖, 吴文帅，一种无接种效应的快速病原菌药敏分析方法, 中国发明专利，受理号：2023112476766；（审查）