科研项目 (2017 年—至今)：
* 承担和主要参与包括国家自然科学基金、广东省“珠江人才计划—青年拔尖人才”项目、广东省现代南药产业技术体系创新团队建设项目等各类型项目 20 项。
论文、专利、计算机软著、获奖 (#, 共同第一作者；*，通讯作者)：
Year: 2025
46. 以主要完成人指导博士后获得’第三届全国博士后创新创业大赛’银奖（项目名称：产业急需大宗药材僵蚕的规范化生产技术体系构建）【占参赛总项目的1.67%（银奖133项/项目总数8006）】
45. Shuangcheng Liang#, Xiaoqin Lin#, Lixin He#, Yizhou Liu, Wenke Xiong, Minghua Lin, Ying Zhang, Ruoting Zhan*, Sidi Wang*, Kui Wang* (通讯作者). Development of an efficient chimeric trifunctional xylanase/glucanase/feruloyl esterase and its application in the bioconversion of corn stover into fermentable sugars. International Journal of Biological Macromolecules, 2025, 307:142236. doi: 10.1016/j.ijbiomac.2025.142236. PubDate: 2025 Mar 18 (中科院一区 Top 期刊, IF=8.5)
44. Yizhou Liu#, Wenke Xiong#, Xinhai Wang#, Shuangcheng Liang, Lixin He, Xiaoqin Lin, Sidi Wang, Ying Zhang*, Ruoting Zhan*, Kui Wang* (通讯作者). Biochemical analyses of a new GH18 chitinase from Beauveria bassiana KW1 and its synergy with a commercial protease on silkworm exuviae hydrolysis. Applied and Environmental Microbiology, 2025, 91(10):e01285-25. DOI: 10.1128/aem.01285-25. (中科院二区，美国微生物学会环境微生物学旗舰期刊，IF=3.7)
43. Wenke Xiong#, Yizhou Liu#, Yingyu Shu#, Shuangcheng Liang, Qiansi Chen, Xueao Zheng, Minghua Li, Ruoting Zhan, Kui Wang* (通讯作者), Ying Zhang*, Sidi Wang*. Development of an efficient bifunctional endoxylanase/feruloyl esterase chimera for co-production of ferulic acid, p-coumaric acid and xylooligosaccharides. Journal of Agricultural and Food Chemistry, 2025, 73(48):30791-30801. DOI: 10.1021/acs.jafc.5c05963. (中科院一区 Top 期刊, IF=6.2)
42. Xiangpeng Yang, Xiangrui Wu, Zezhou Jiang, Kui Wang, Yizhou Liu, Zongmin Qin, Sufang Zhang, Yuhuan Liu, Lichuang Cao. Fine-tuning the active-site microenvironment of β-galactosidase to enhance the synthesis ability of galactooligosaccharides while minimizing the impairment to transglycosylation activity. Journal of Agricultural and Food Chemistry. 2025, 73(19):11866-11877. doi: 10.1021/acs.jafc.5c00679. PubDate: 2025 Apr 22.
41. 王魁, 詹若挺, 熊文科, 刘逸洲, 刘军民, 汪思迪.一株对多个家蚕品种具有高毒力的球孢白僵菌菌株及其在僵蚕生产中的应用. 中国发明专利, 2025. (ZL2024114541618).
40. 王魁, 黄志, 詹若挺, 莫凯, 麦远娜, 林晓琴, 熊文科. 一种大幅提高家蚕死亡时间集中度的球孢白僵菌菌剂及高品质僵蚕生产方法. 中国发明专利（申请号：202510263535.6）.
39. 詹若挺, 王魁, 熊文科, 刘逸洲, 刘军民, 汪思迪. 一株适用于规模化生产高品质僵蚕的菌株及其应用. 中国发明专利, 2025. (ZL202411814403.X)
38. 王魁，詹若挺，刘军民，陈昱帆，陈立凯，王丽平. 僵蚕规范化生产白僵菌孢子高效制备管理系统. 计算机软件著作权（登记号：2025SR1749556）（证书号：软著登字第16405754号）
37. 王魁，詹若挺，刘军民，陈立凯，王丽平，陈昱帆. 僵蚕规范化生产白僵菌侵染家蚕工艺管理系统. 计算机软件著作权（登记号：2025SR1749557）（证书号：软著登字第16405755号）
36. 王魁，詹若挺，刘军民，陈立凯，王丽平，陈昱帆. 僵蚕规范化生产僵蚕干燥管理系统. 计算机软件著作权（登记号：2025SR1672928）（证书号：软著登字第16329126号）
35. 王魁，詹若挺，刘军民，王丽平，陈昱帆，陈立凯. 僵蚕规范化生产僵蚕储存管理系统. 计算机软件著作权（登记号：2025SR1672921）（证书号：软著登字第16329119号）
34. 詹若挺，王魁，刘军民，王丽平，陈立凯，陈昱帆. 僵蚕规范化生产僵蚕溯源管理系统. 计算机软件著作权（登记号：2025SR1564153）（证书号：软著登字第16220351号）
33. 詹若挺，王魁，刘军民，陈昱帆，陈立凯，王丽平. 僵蚕规范化生产养蚕管理系统. 计算机软件著作权（登记号：2025SR1565408）（证书号：软著登字第16221606号）
32. 詹若挺，王魁，刘军民，陈立凯，王丽平，陈昱帆. 僵蚕规范化生产种桑管理系统. 计算机软件著作权（登记号：2025SR1680993）（证书号：软著登字第16337191号）
31. 参编国家级规划教材：
（1）担任学术秘书：科学出版社“十四五”普通高等教育规划教材《药用植物栽培学》，2025年。
（2）担任编委：全国中医药行业高等教育“十五五”规划教材《中药生物技术》，2025年。
Year: 2024
30. Qibin Lin#, Xiaoru Wang#, Liang Zhang#, Yingyu Shu, Yurou Zhang, Ruoting Zhan*, Kui Wang* (通讯作者). Investigations in mutation breeding and culturing media by Xanthophyllomyces dendrorhous. Biocatalysis and Agricultural Biotechnology, 2024. DOI: 10.1016/j.bcab.2023.103008. PubDate: 2024.01.01. Online: 2023.10.29 (Accepted, IF=4.0)
29. Junjie Shi, Lihua Peng, Weixuan Chen, Weilin Qiao, Kui Wang, Yueyang Xu, Jinle Cheng. Evaluation of chemical components and quality in Xinhui Chenpi (Citrus reticulata 'Chachi') with two different storage times by GC-MS and UPLC. Food Science & Nutrition, 2024, 12(7): 5036-5051. doi: 10.1002/fsn3.4154. PubDate: 2024 Apr 10.
Year: 2023
28. Ying Liu# , Ying Zhang#, Minghuan Wang, Lisheng Wang, Wanting Zheng, Qiannuo Zeng, Kui Wang* (通讯作者). Comparison of the basic processes of aerobic, anaerobic and aerobic-anaerobic coupling composting of Chinese medicinal herbal residues. Bioresource Technology, 2023, 379: 128996. DOI: 10.1016/j.biortech.2023.128996. PubDate (Online): 2023.04.01 (中科院一区 Top 期刊, IF=11.4)
27. Wei Xie, Ying Zhang, Lin Zhang, Liangkai Cheng, Zhongqiu Liu, Kui Wang* (通讯作者), Caiyan Wang*. Application prospects and enzyme enginerring of GH10 family xylanase. Biotechnology and Genetic Engineering Reviews, 2023. (Accepted) (中科院三区, IF=3.2)
26. Yurou Zhang#, Yun Liu#, Chunxiu Zeng, Yinyu Shu, Xiaoru Wang, Shuangcheng Liang, Sidi Wang*, Ruoting Zhan*, Kui Wang* (通讯作者). Characterization of two novel highly active glycoside hydrolase family 53 endo-1,4-β-galactanases and their synergism with other carbohydrases in plant polysaccharide decomposition. International Journal of Biological Macromolecules, 2023, 224 (2023): 653–666. DOI: 10.1016/j.ijbiomac.2022.10.154. PubDate: 2023.01.01. Online: 2022.10.22 (中科院一区 Top 期刊, IF=8.2)
Year: 2022
25. Ruiqin Zhang, Dongxia Lin, Liang Zhang, Ruoting Zhan, Sidi Wang*, Kui Wang* (通讯作者). Molecular and biochemical analyses of a novel trifunctional endoxylanase/endoglucanase/feruloyl esterase from the human colonic bacterium Bacteroides intestinalis DSM 17393. Journal of Agricultural and Food Chemistry, 2022, 70: 4044−4056. DOI: 10.1021/acs.jafc.2c01019. PubDate (Online): 2022.03.22 (中科院一区 Top 期刊, Supplemetary Cover Article【内封面论文】, IF=6.1)
24. 王魁，张瑞勤，汪思迪. 一种兼具木聚糖酶、葡聚糖酶和阿魏酸酯酶活性的三功能酶Bi76及其应用. 中国发明专利, 2022. (申请号：2022102434832)
23. Lin Zhang, Wei Xie, Xiaojun Teng, Kui Wang, Wei Xie*, Caiyan Wang*. Improving the extraction of active ingredients from medicinal plants by XynA modification. Journal of Chemistry, 2022, 2022: 2483797. DOI: 10.1155/2022/2483797. PubDate (Online): 2022.02.03 (JCR Q3, IF=3.241)
Year: 2021
22. 王魁，刘云，汪思迪，张亮，张瑞勤，陈茜. 一种特异性产生木寡糖的木聚糖酶BgXyn8A及其基因与应用. 中国发明专利，2024年（ZL202110681603.2）
21. Wei Xie, Qi Yu, Ruiqing Zhang, Yun Liu, Ruoting Cao, Sidi Wang, Ruoting Zhan, Zhongqiu Liu, Kui Wang* (通讯作者), Caiyan Wang*. Insights into the catalytic mechanism of a novel XynA and structure-based engineering for improving bifunctional activities. Biochemistry, 2021, 60(26):2071−2083. DOI: 10.1021/acs.biochem.1c00134. PubDate (Online): 2021.06.22 (JCR Q3, IF=3.321)
20. MinghuanWang, Ying Liu, Shanqi Wang, Kui Wang, Ying Zhang*. Development of a compound microbial agent beneficial to the composting of Chinese medicinal herbal residues. Bioresource Technology, 2021, 330: 124948. DOI: 10.1016/j.biortech.2021.124948. PubDate (Online): 2021.03.10 (中科院一区 Top 期刊, IF=11.888)
19. Xi Chen#, Xinhai Wang#, Yun Liu, Ruoting Cao, Sidi Wang, Ruoting Zhan, Kui Wang* (通讯作者). Biochemical analyses of a novel thermostable GH5 endo beta-1,4-mannanase with minor beta-1,4-glucosidic cleavage activity from Bacillus sp. KW1 and its synergism with a commercial alpha-galactosidase on galactomannan hydrolysis. International Journal of Biological Macromolecules, 2021, 166: 778−788. DOI: 10.1016/j.ijbiomac.2020.10.235. PubDate: 2021.01.01. Online: 2020.11.02 (中科院一区 Top 期刊, IF=8.025)
Year: 2020
18. Xiongyu Ji, Shumin Lin, Yuanyuan Chen, Jiawei Liu, Xiaoyun Yun, Tiancheng Wang, Jialiang Qin, Chaoquan Luo, Kui Wang, Zhongxiong Zhao, Ruoting Zhan*, Hui Xu*. Identification of alpha-amyrin 28-carboxylase and glycosyltransferase from Ilex asprella and production of ursolic acid 28-O-beta-D-glucopyranoside in engineered yeast. Frontiers in Plant Science, 2020, 11: 612. (JCR Q1, IF=5.753)
Year: 2019
17. Qibin Lin#, Sidi Wang#, Meiling Wang, Ruoting Cao, Ruiqing Zhang, Ruoting Zhan, Kui Wang*(通讯作者). A novel glycoside hydrolase family 42 enzyme with bifunctional beta-galactosidase and alpha-L-arabinopyranosidase activities and its synergistic effects with cognate glycoside hydrolases in plant polysaccharides degradation. International Journal of Biological Macromolecules, 2019, 140: 129−139. DOI: 10.1016/j.ijbiomac.2019.08.037. PubDate (Online): 2019.08.10 (中科院一区 Top 期刊, IF=5.162)
16. Kui Wang#,* (通讯作者), Ruoting Cao#, Meiling Wang, Qibin Lin, Ruoting Zhan, Hui Xu, Sidi Wang*. A novel thermostable GH10 xylanase with activities on a wide variety of cellulosic substrates from a xylanolytic Bacillus strain exhibiting significant synergy with commercial Celluclast 1.5 L in pretreated corn stover hydrolysis. Biotechnology for Biofuels, 2019, 12: 48. DOI: 10.1186/s13068-019-1389-8. PubDate (Online): 2019.03.09 (中科院一区 Top 期刊, IF=4.815)
15. Zhixue Wu#, Hui Xu#, Meiling Wang, Ruoting Zhan, Weiwen Chen, Kui Wang* (通讯作者), Jiangyong Gu*. Molecular docking and molecular dynamics studies on selective synthesis of alpha-amyrin and beta-amyrin by oxidosqualene cyclases from Ilex asprella. International Journal of Molecular Sciences, 2019, 20: 3469. DOI: 10.3390/ijms20143469. PubDate (Online): 2019.07.15 (JCR Q2, IF=4.556)
14. Yuanyuan Chen, Meihong Lai, Xiaoyun Yun, Kui Wang* (通讯作者), Hui Xu*. Discovery and functional analysis of a cytochrome P450 gene involved in C-28 oxidation of the triterpenoids from Ilex pubescens. Journal of China Pharmaceutical University, 2019, 50: 459−467.
陈媛媛，赖美红，贠小芸，王魁* (通讯作者)，徐晖*. 毛冬青三萜C-28位氧化修饰相关CYP450基因的发掘和功能鉴定. 中国药科大学学报, 2019, 50: 459−467.
Year: 2017
13. Daniel Wefers, Janaina J. V. Cavalcante, Rachel R. Schendel, Jaigeeth Deveryshetty, Kui Wang, Zdzislaw Wawrzak, Roderick I. Mackie, Nicole M. Koropatkin, Isaac Cann*. Biochemical and structural analyses of two cryptic esterases in Bacteroides intestinalis and their synergistic activities with cognate xylanases. Journal of Molecular Biology, 2017, 429(16): 2509−2527. (JCR Q1, IF=4.894)
12. 王耀辉，谢建华，王魁，李小明，何志梅，徐莉敏，王源丰. 用于生产具有耐温性脂肪酶的基因. 中国发明专利, 2017. (公开号：CN106868026A)
Year: 2016
11. Kui Wang, Gabriel Vasconcelose Pereira, Janaina Cavalcante, Meiling Zhang, Roderick Mackie, Isaac Cann*. Bacteroides intestinalis DSM 17393, a member of the human colonic microbiome, upregulates multiple endoxylanases during growth on xylan. Scientific Reports, 2016, 6: 34360. (JCR Q1, IF=5.228).
Year: 2013
10. Sidi Wang, Kui Wang, Yuhuan Liu*. Isolation and characterization of a novel organic solvent-tolerant and halotolerant esterase from a soil metagenomic library. Journal of Molecular Catalysis B-Enzymatic, 2013, 95: 1−8. (IF=2.745)
Year: 2012
9. Kui Wang, Yi Lu, Weiqu Liang, Sidi Wang, Yang Jiang, Rui Huang, Yuhuan Liu*. Enzymatic synthesis of Galacto-oligosaccharides in an organic-aqueous biphasic System by a novel beta-galactosidase from a metagenomic library. Journal of Agricultural and Food Chemistry, 2012, 60: 3940−3946. (JCR Q1, 中科院一区, IF=3.154).
8. Kui Wang, Sidi Wang, Rui Huang, Yuhuan Liu. Advances of metagenomics in discovering novel biocatalysts. Chinese Journal of Biotechnology, 2012, 28: 420−431.
7. Shang-ke Chen, Kui Wang, Yuhuan Liu*, Xiaopeng Hu*. Crystallization and preliminary X-ray analysis of a novel halotolerant feruloyl esterase identified from a soil metagenomic library. Acta Crystallographica Section F, 2012,68: 767−770. (IF=0.52).
6. 刘玉焕，刘孝龙，王魁，鲁毅.一种具有高效转糖基β-半乳糖苷酶的新基因及应用. 中国发明专利, 2012. (授权公开号：CN 102154239 B)
Year: 2011
5. Liping Zhang#, Kui Wang# (共同第一作者), Zhongxing Mo, Yuhuan Liu*, Xiaopeng Hu*. Crystallization and preliminary X-ray analysis of a cold-active beta-galactosidase from the psychrotrophic and halotolerant Planococcus sp. L4. Acta Crystallographica Section F, 2011, 67: 911−913. (IF=0.56)
4. Xinjiong Fan, Xiaolong Liu, Kui Wang, Sidi Wang, Rui Huang, Yuhuan Liu*. Highly soluble expression and molecular characterization of an organic solvent-stable and thermotolerant lipase originating from the metagenome. Journal of Molecular Catalysis B-Enzymatic, 2011, 72, 319−326. (IF=2.823)
3. 王魁，刘玉焕. 一种新型 β-半乳糖苷酶高效转糖基化作用的研究. 第八届中国酶工程学术研讨会论文，2011.
Year: 2010
2. Kui Wang#, Gang Li#, Shi Qin Yu, Chen Ting Zhang, Yuhuan Liu*. A novel metagenome-derived beta-galactosidase: gene cloning, overexpression, purification and characterization. Applied Microbiology and Biotechnology, 2010, 88: 155−165. (JCR Q2, IF=3.28).
Year: 2008
1. Gang Li#, Kui Wang# (共同第一作者), Yuhuan Liu*. Molecular cloning and characterization of a novel pyrethroid-hydrolyzing esterase originating from the metagenome. Microbial Cell Factories, 2008, 7: 38. (JCR Q2, IF=3.34).
