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(23) Zhang, Y.; Lan, Y.; Fan, R.; Feng, L.; Wang, G.; Wu, X,; Wen, L.; Duan, Z.; Xia, Y.;Wang, X.; Zhang, L.; Zhou, L.; Tan, M.;Liao, C.; Lu, X. Biocatalytic- and Chemoproteomic-Guided Discovery of a PHGDH Inhibitor from Chemoenzymatic-Promoted DNA-Encoded Libraries Selection Platform. J. Am. Chem. Soc. 2025https://pubs.acs.org/doi/10.1021/jacs.5c14634  

(22) Li, Y.; Li, Q.; Zhang, R.; Liao, C. Stereoselective De Novo Construction of Cyclopropane by a Mutifunctional Class Ⅰ Aldolase.  J. Am. Chem.  Soc. 2025https://pubs.acs.org/doi/10.1021/jacs.5c13402

(21) Zhang, J.; Yu, K.; Xu, Y.; Zhao, W.; Li, Y.; Wang, Y.; Seebeck, F. P.; Chen, X.; Liao, C. A Robust Platform Streamlining Aromatic Noncanonical Amino Acid Biosynthesis and Genetic Code Expansion in Escherichia coli. Nat. Commun. 2025. https://doi.org/10.1038/s41467-025-63679-6

(20) Li, Q.; Zhang, R.; Lan, Y.; Li, Y.; Tang, C.; Ke, C.; Ye, Y.;  Liao, C. Asymmetric Radical Alkylation Enabled by Synergistic Photoredox Enamine Biocatalysis. ChemCatChem. 2025. https://doi.org/10.1002/cctc.202501219

              

                                                                                              

(19) Yang, H.; Li, Q.; Wang, S.; Zhang, R.; Sheng, X.;  Liao, C. Bimolecular Nucleophilic Substitution (SN2) Reaction Catalyzed by L-Threonine Aldolase.  J. Am. Chem. Soc. 2025https://doi.org/10.1021/jacs.5c07660


(18) Lan, Y.; Zhang, C.; Tang, C.; Ye, Y.; Zhang R.; Sheng, X.; Liao, C. Semirational Protein Engineering of a Decarboxylative Aldolase for Regiodivergent and Stereodivergent Synthesis of Cyclic Imino Acids. Angew. Chem. Int. Ed. 2025. https://doi.org/10.1002/anie.202500080

(17) Yu, K.; Liu, Y.; Zhang, Z.; Zhao,W.; Mao, Y.; Liao,C. Robust Whole-Cell-Based Chemoenzymatic Synthesis of Site-Selective Deuterated α-Hydroxy Acids and α-Amino Acids. Adv. Synth. Catal. 2024. https://doi.org/10.1002/adsc.202401330

(16) Lian, X.; Mao, Y.; Fu, Z.; Zhang, W.; Chen, J.; Zhuo, D.; Zheng, M.; Wu, J.; Liao, C. Siteselective and Enantiocomplementary C(Sp3)–H Oxyfunctionalization for Synthesis of α-Hydroxy Acids. ACS Catal. 2024https://doi.org/10.1021/acscatal.4c00398.


(15) Luo, Z.; Li, Q.; Yang, H.; Li, Y.; Liu, Y.; Tang, C.; Liu, J.; Ke, C.; Ye, Y.; Zhang, R.; Liao, C. Vicinal Diones and α-Keto Esters as Electrophiles of Aldolases for Stereoselective Construction of Tertiary Alcohols. ChemCatChem 2024https://doi.org/10.1002/cctc.202400337.

(14) Zhang, R.; Zhang, C.; Tan, J.; He, Y.; Zhuo, D.; Zhang, J.; Luo, Z.; Li, Q.; Yao, J.; Ke, C.; Tang, C.; Ye, Y.; He, S.; Sheng, X.; Liao, C. Enzymatic Synthesis of Noncanonical α‐Amino Acids Containing γ‐Tertiary Alcohols. Angew. Chem. Int. Ed. 2023. https://doi.org/10.1002/anie.202318550.

(13) Liu, Y.; Fu, Z.; Dong, H.; Zhang, J.; Mao, Y.; Zheng, M.; Liao, C. Asymmetric C1 Extension of Aldehydes through Biocatalytic Cascades for Stereodivergent Synthesis of Mandelic Acids. Angew. Chem. Int. Ed. 2023. https://doi.org/10.1002/anie.202300906.

(12) Mao, Y.; Zhang, W.; Fu, Z.; Liu, Y.; Chen, L.; Lian, X.; Zhuo, D.; Wu, J.; Zheng, M.; Liao, C. Versatile Biocatalytic C(Sp3)−H Oxyfunctionalization for the Site‐ Selective and Stereodivergent Synthesis of α‐ and β‐Hydroxy Acids. Angew. Chem. Int. Ed. 2023. https://doi.org/10.1002/anie.202305250.

(11) Chen, L.; Zhang, Z.; Li, Z.; Li, R.; Huo, R.; Chen, L.; Wang, D.; Luo, X.; Chen, K.; Liao, C.; Zheng, M. Learning Protein Fitness Landscapes with Deep Mutational Scanning Data from Multiple Sources. Cell Syst. 2023. https://doi.org/10.1016/j.cels.2023.07.003.

(10) Zhang, R.; Tan, J.; Luo, Z.; Dong, H.; Ma, N.; Liao, C. Stereo-Selective Synthesis of Non-Canonical γ-Hydroxy-α-Amino Acids by Enzymatic Carbon–Carbon Bond Formation. Catal. Sci. Technol. 2021. https://doi.org/10.1039/d1cy00955a.

(9) Peng, J.; Liao, C.; Bauer, C.; Seebeck, F. P. FluorinatedS‐Adenosylmethionine as a Reagent for Enzyme‐Catalyzed Fluoromethylation. Angew. Chem. Int. Ed. 2021. https://doi.org/10.1002/anie.202108802.

(8) Liao, C.; Seebeck, F. P. Asymmetric β-Methylation of l- and d-α-Amino Acids by a Self-Contained Enzyme Cascade. Angew. Chem. Int. Ed. 2020. https://doi.org/10.1002/anie.201916025.

(7) Goncharenko, K. V.; Flückiger, S.; Liao, C.; Lim, D.; Stampfli, A. R.; Seebeck, F. P. Selenocysteine as a Substrate, an Inhibitor and a Mechanistic Probe for Bacterial and Fungal Iron-Dependent Sulfoxide Synthases. Chem. Eur. J. 2020. https://doi.org/10.1002/chem.201903898.

(6) Liao, C.; Seebeck, F. P. S -Adenosylhomocysteine as a Methyl Transfer Catalyst in Biocatalytic Methylation Reactions. Nat. Catal. 2019. https://doi.org/10.1038/s41929-019-0300-0.

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(5) Liao, C.; Seebeck, F. P. In Vitro Reconstitution of Bacterial DMSP Biosynthesis. Angewandte Chemie 2019https://doi.org/10.1002/anie.201814662.

(4) Gamage, A. M.; Liao, C.; Cheah, I. K.; Chen, Y.; Lim, D. R. X.; Ku, J. W. K.; Chee, R. S. L.; Gengenbacher, M.; Seebeck, F. P.; Halliwell, B.; Gan, Y.-H. The Proteobacterial Species Burkholderia Pseudomallei Produces Ergothioneine, Which Enhances Virulence in Mammalian Infection. FASEB J. 2018. https://doi.org/10.1096/fj.201800716.

(3) Liao, C.; Seebeck, F. P. Convergent Evolution of Ergothioneine Biosynthesis in Cyanobacteria. ChemBioChem 2017. https://doi.org/10.1002/cbic.201700354.

(2) Ruan, J.; Liao, C.; Ye, Y.; Lin, G. Lack of Metabolic Activation and Predominant Formation of an Excreted Metabolite of Nontoxic Platynecine-Type Pyrrolizidine Alkaloids. Chem. Res. Toxicol. 2013. https://doi.org/10.1021/tx4004159.

(1) Liao, C.-S.; Tang, C.-P.; Yao, S.; Ye, Y. Humulane-Type Sesquiterpenoids from Pilea Cavaleriei Subsp. Crenata†. Org. Biomol. Chem. 2013. https://doi.org/10.1039/c3ob40872h.