刘庆,中国水稻研究所副研究员,主要研究方向有水稻基因组学及生物信息学。发表SCI论文10篇,主持国家自然科学基金1项,获国家授权发明专利2项。针对基因组编辑后大样本序列鉴定耗时费力费钱的问题,开发了Hi-TOM工具平台,利用该平台可以高通量、高效和经济地解析大量样本的精准突变信息,成功实现了该科研成果的转化应用,现已被广泛应用于在动物、植物、微生物和人类细胞中基因组编辑突变的检测。
教育背景及工作经历
2005-2009年 华南农业大学 农学学士
2009-2014年 华南农业大学 遗传学博士
2014-2015年 中国水稻研究所 研究实习员
2015-2020年 中国水稻研究所 助理研究员
2021年-至今 中国水稻研究所 副研究员
申请项目情况
国家自然基金“利用基因编辑技术重塑水稻遗传重组分布研究”(32472187),2025年01月至2028年12月,主持;
浙江省自然科学基金“利用基因工程技术快速创制高重组频率的水稻温敏不育系”(LTGN24C130005), 2024年01月至2026年12月,主持;
国家重点研发项目“农作物倍性与染色体工程技术”(2023YFD1200800),2023年12月至2028年11月, 主持;
国家自然科学基金“杂交水稻遗传重组发生规律的研究”(31901523),2020年01月至2022年12月,主持;
科技部重点研发项目“水稻、大豆品种 DNA 指纹分子鉴定技术研究”(2017YFD0102002),2017年07月至2020年12月,参与。
论文发表情况
Chaolei Liu†, Song Yan†, Fangming Mao, Tingting Sun, Huan Liang, Qing Liu, Qian Qian*, Kejian Wang*. Large-scale production of rice haploids by combining superior haploid inducer with PTGMS lines, Plant Communications, 2024, DOI:10.1016/j.xplc.2024.101067.
Tingting Sun†, Qing Liu†*, Xi Chen, Fengyue Hu & Kejian Wang*. Hi-TOM 2.0: an improved platform for high-throughput mutation detection, Science China Life Sciences, 2024,67(7):1532-1534, DOI:10.1007/s11427-024-2555-x.
Guoning Zhu, Lingling Zhang, Liqun Ma, Qing Liu, Kejian Wang, Jinyan Li, Guiqin Qu, Benzhong Zhu, Daqi Fu, Yunbo Luo, Hongliang Zhu. Efficient large fragment deletion in plants: double pairs of sgRNAs are better than dual sgRNAs, Horticulture Research, 2023,10(10), DOI:10.1093/hr/uhad168.
Chaolei Liu, Zexue He, Yan Zhang, Fengyue Hu, Mengqi Li, Qing Liu, Yong Huang, Jian Wang, Wenli Zhang*, Chun Wang* and Kejian Wang*. Synthetic apomixis enables stable transgenerational transmission of heterotic phenotypes in hybrid rice, Plant Communications, 2023, 4(2): 100470, DOI:10.1016/j.xplc.2022.100470.
Xin Wei, Qing Liu, Tingting Sun, Xiaozhen Jiao, Chaolei Liu, Fengyue Hu, Xi Chen and Kejian Wang*. Manipulation of genetic recombination by editing the transcriptional regulatory regions, Plant Communications, 2023, 4(2): 100474, DOI:10.1016/j.xplc.2022.100474.
Jinpeng Zou, Xiangbing Meng, Qing Liu, Meiqi Shang, Kejian Wang, Jiayang Li, Hong Yu & Chun Wang. Improving the efficiency of prime editing with epegRNAs and hightemperature treatment in rice, SCIENCE CHINA life sciences, 2022, 65(11):2328-2331, DOI:10.1007/s11427-022-2147-2.
李慧颖,刘庆,郭旻,王克剑,严长杰,王春. Hi-Meth:特定位点 DNA 甲基化高通量检测平台, 生物工程学报, 2022, 38(8): 3049-3061, DOI:10.13345/j.cjb.220190.
沈兰†, 华宇峰†, 付亚萍†, 李健†, 刘庆, 焦晓真, 辛高伟, 王俊杰, 王兴春, 严长杰*, 王克剑*. 利用CRISPR/Cas9多基因编辑系统在水稻中快速引入遗传多样性, 中国科学:生命科学, 2017, 47(11):1186-1195, DOI:10.1360/N052017-00166.
Jun Ren#, Xiangbing Meng#, Fengyue Hu#, Qing Liu, Yuexuan Cao, Huiying Li,Changjie Yan, Jiayang Li, Kejian Wang, Hong Yu* & Chun Wang*. Expanding the scope of genome editing with SpG and SpRY, Science China Life sciences, 2021, 64(10):1784-1787, DOI:10.1007/s11427-020-1883-5.
Qing Liu, Xiaozhen Jiao, Xiangbing Meng, Chun Wang, Cao Xu, Zhixi Tian, Chuanxiao Xie, Genying Li, Jiayang Li, Hong Yu & Kejian Wang. FED: a web tool for foreign element detection of genome-edited organism, Science China Life sciences, 2021, 64(1):167-170, DOI:10.1007/s11427-020-1731-9.
Ling Wang*†, Qing Liu†, Shuailing Ge, Wenhao Liang, Weiyang Liao, Wen Li, Guiai Jiao, Xiangjin Wei, Gaoneng Shao, Lihong Xie, Zhonghua Sheng, Shikai Hu, Shaoqing Tang* and Peisong Hu*. Genomic footprints related with adaptation and fumonisins production in Fusarium proliferatum, Frontiers in microbiology, 2022, DOI:10.3389/fmicb.2022.1004454.
Liu, Q., Jiao, X., Meng, X., Wang, C., Xu, C., Tian, Z., Xie, C., Li, G., Li, J., Yu, H., and Wang, K.* (2020). FED: a web tool for foreign element detection of genome-edited organism. Science China Life Sciences, 64(1):167-170.
Li, S. , Shen, L. , Hu, P. , Liu, Q. , X Zhu, & Qian, Q. , et al. (2020). Developing disease-resistant thermosensitive male sterile rice by multiplex gene editing. Journal of integrative plant biology, 61(12): 1201-1205, SCIE MEDLINE ISTIC CSCD BP, by the Central Public-interest Scientific Institution Basal Research Fund of China National Rice Research Institute.
Wang C., Liu Q., Shen Y., Hua Y.g, Wang J., Lin J., Wu M., Sun T., Cheng Z., Mercier R., Wang K.* (2019). Clonal seeds from hybrid rice by simultaneous genome engineering of meiosis and fertilization genes. Nature Biotechnology, 37: 283-286. 封面文章.
Liu, Q.#, Wang, C.#, Jiao, X., Zhang, H., Song, L., Li, Y., Gao, C., and Wang, K*. (2019). Hi-TOM: a platform for high-throughput tracking of mutations induced by CRISPR/Cas systems. Science China Life Sciences, 62:1-7. 封面文章。
Li S#., Shen L#., Hu P., Liu Q., Zhu X., Qian Q., Wang K*., and Wang Y*. Developing disease-resistant thermosensitive male sterile rice by multiplex gene editing, Journal of Integrative Plant Biology, 2019, 61(12): 1201-1205.
Hu, X. , Meng, X. , Liu Q., Li, J. , & Wang, K. .(2018). Increasing the efficiency of crispr‐cas9‐vqr precise genome editing in rice. Plant Biotechnology Journal, 16(1).
Meng, X. , Hu, X. , Liu, Q. , Song, X. , Gao, C. , & Li, J. , et al. (2018). Robust genome editing of crispr-cas9 at nag pams in rice. Science China Life Sciences, 061(001), P.122-125.
Liang, J. , Guo, S. , Sun, B. , Liu, Q. , Chen, X. , & Peng, H. , et al. (2018). Constitutive expression of rel1 confers the rice response to drought stress and abscisic acid. Rice, 11(1).
Shen, L. , Hua, Y. , Fu, Y. , Li, J. , Liu, Q. , & Jiao, X. , et al. (2017). Rapid generation of genetic diversity by multiplex crispr/cas9 genome editing in rice. Science China Life Sciences.
Qiu, J., Hou, Y., Tong, X. Wang, Y., Lin, H., Liu, Q. et al.(2016). Quantitative phosphoproteomic analysis of early seed development in rice (Oryza sativa L.). Plant Molecular Biology 90, 249–265. https://doi.org/10.1007/s11103-015-0410-2
Hu, X. , Wang, C. , Liu, Q. , Fu, Y. , & Wang, K. (2016). Targeted mutagenesis in rice using crispr-cpf1 system. Journal of Genetics and Genomics, 44(1), 3.
Hu, X., Wang, C., Fu, Y., Liu, Q., Jiao X., Wang, K.(2016). Expanding the range of crispr/cas9 genome editing in rice. Molecular Plant(6), 943-945.
- Wang, K., Wang, C. , Liu, Q., Liu, W. , & Fu, Y. (2015). Increasing the genetic recombination frequency by partial loss of function of the synaptonemal complex in rice. Molecular Plant(8), 1295-1298.