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Microfluidic device featuring micro-constrained channels for multi-parametric assessment of cellular biomechanics and high-precision mechanical phenotyping of gastric cells Anal. Chim. Acta (IF 6.2) Pub Date : 2024-03-12 Yang Heng, Xinyu Zheng, Youyuan Xu, Jiaqi Yan, Ying Li, Lining Sun, Hao Yang
Cellular biomechanics play a significant role in the regulation of cellular physiological and pathological processes. In recent years, multiple methods have been developed to evaluate cellular biomechanics, such as atomic force microscopy (AFM), microscope aspiration, and magnetic tweezers. However, most of these methods only focus on a single parameter and cannot automate the process at a high-efficiency
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Isothermal strand displacement polymerase reaction (ISDPR)-assisted microchip electrophoresis for highly sensitive detection of cancer associated microRNAs Anal. Chim. Acta (IF 6.2) Pub Date : 2024-03-12 Jingyi Chen, Jingzi Zhang, Qihui Xie, Zhaohui Chu, Yuqi Lu, Fan Zhang, Qingjiang Wang
More and more studies have found that microRNAs (miRNAs) are markers of cancer, and detection of miRNAs is beneficial for early diagnosis and prognosis of cancer. In this paper, the isothermal strand displacement polymerase reaction (ISDPR), which is an enzyme-assisted nucleic acid amplification method, was studied to combine with microchip electrophoresis (MCE) for a simultaneously detection of two
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Quantifying exhaled acetone and isoprene through solid phase microextraction and gas chromatography-mass spectrometry Anal. Chim. Acta (IF 6.2) Pub Date : 2024-03-12 Eray Schulz, Mark Woollam, Sneha Vashistha, Mangilal Agarwal
Acetone, isoprene, and other volatile organic compounds (VOCs) in exhaled breath have been shown to be biomarkers for many medical conditions. Researchers use different techniques for VOC detection, including solid phase microextraction (SPME), to preconcentrate volatile analytes prior to instrumental analysis by gas chromatography-mass spectrometry (GC-MS). These techniques include a previously developed
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β-Glucuronidase-triggered reaction for fluorometric and colorimetric dual-mode assay based on the in situ formation of silicon nanoparticles Anal. Chim. Acta (IF 6.2) Pub Date : 2024-03-11 Yue Li, Weiping Liu, Xinxin Jiang, Hongmei Liu, Sikai Wang, Xiaoqian Mao, Ruyu Bai, Yulu Wen, Xiaojun Luo, Guoqi Zhang, Yan Zhao
β-Glucuronidase (GUS) is considered as a promising biomarker for primary cancer. Thus, the reliable detection of GUS has great practical significance in the discovery and diagnosis of cancer. Compared with traditional organic probes, silicon nanoparticles (Si NPs) have emerged as robust optical nanomaterials due to their facile preparation, superior photobleaching resistance and excellent biocompatibility
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A hydrogel-based ratiometric fluorescent sensor relying on rhodamine B labelled AIE-featured hyperbranched poly(amido amine) for heparin detection Anal. Chim. Acta (IF 6.2) Pub Date : 2024-03-11 Cheng Li, Bai-Tong Liu, Yi-Ting Wang, Tian-Jiao Zhang, Xiaochun Zheng, Li Chen, Shaobo Li, Xiaoxian Tian, Dequan Zhang, Yong Wang
The fluorescent flexible sensor for point-of-care quantification of clinical anticoagulant drug, Heparin (Hep), is still an urgent need of breakthrough. In this research, a hyperbranched poly(amido amine) (HPA) was decorated with tetraphenylethene (TPE) and Rhodamine B (RhB), constructing a ratiometric fluorescent sensor (TR-HPA) for Hep. When the sensor was exposed to Hep, the TPE units within the
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In vitro selection of a trans aptamer complex for target-responsive fluorescence activation Anal. Chim. Acta (IF 6.2) Pub Date : 2024-03-11 Soyeon V. Park, Byunghwa Kang, Minjong Lee, Hyebin Yoo, Hyesung Jo, Sungwook Woo, Seung Soo Oh
Most biological molecular complexes consist of multiple functional domains, yet rationally constructing such multifunctional complexes is challenging. Aptamers, the nucleic acid-based functional molecules, can perform multiple tasks including target recognition, conformational changes, and enzymatic activities, while being chemically synthesizable and tunable, and thus provide a basis for engineering
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Construction of a glycosylation-mediated fluorescent biosensor for label-free measurement of site-specific 5-hydroxymethylcytosine in cancer cells with zero background signal Anal. Chim. Acta (IF 6.2) Pub Date : 2024-03-11 Wei Wang, Su Jiang, Yue-ying Li, Yun Han, Meng Liu, Ying-ying Meng, Chun-yang Zhang
5-hydroxymethylcytosine (5hmC) as an epigenetic modification can regulate gene expression, and its abnormal level is related with various tumor invasiveness and poor prognosis. Nevertheless, the current methods for 5hmC assay usually involve expensive instruments/antibodies, radioactive risk, high background, laborious bisulfite treatment procedures, and non-specific/long amplification time. We develop
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Ultrasensitive cortisol electrochemical immunosensor amplifying by Au single-atom nanozymes and HRP enzymes Anal. Chim. Acta (IF 6.2) Pub Date : 2024-03-11 Yongling Du, Min Guo, Yan Chen, Xiaohui Mo, Junlei Cao, Fangdi Hu
Cortisol, a corticosteroid hormone as a primary stress hormone response to internal and external stress, has been regarded as a gold standard reliable biomarker to evaluate human mental stress. The double enzymes strategy, using nanozyme and enzyme amplifying the electrochemical signal, has been widely used to improve the performance of electrochemical biosensors. An ultra-sensitive electrochemical
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A new coaxial flow-through probe for electromembrane extraction of methadone from clinical samples on-line coupled to capillary electrophoresis Anal. Chim. Acta (IF 6.2) Pub Date : 2024-03-07 František Opekar, Petr Tůma
A new design of a flow-through coaxial electromembrane extraction (EME) probe that can be on-line coupled with CE instrument is described and tested. The supporting base of the probe is a PDMS microchip with T-shaped channels into which two coaxially arranged capillaries for inlet and outlet solutions are inserted. The extraction part of the probe is a porous polypropylene hollow fiber, sealed at one
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Analysis of the response to cigarette smoke exposure in cell coculture and monoculture based on bionic-lung microfluidic chips Anal. Chim. Acta (IF 6.2) Pub Date : 2024-03-06 Zezhi Li, Boyang Feng, Xiang Li, Junwei Zhao, Kejian Liu, Fuwei Xie, Jianping Xie
toxicity assessment studies with various experimental models and exposure modalities frequently generate diverse outcomes. In the prevalent experimental, aerosol pollutants are dissolved in culture medium through capture for exposure to two-dimensional planar cellular models in multiwell plates via immersion. However, this approach can generate restricted and inconclusive experimental data, significantly
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Determination of ultra-trace Te species in open ocean waters based on Mg(OH)2 coprecipitation, anion exchange resin column separation and inductively coupled plasma sector-field mass spectrometry using a 125Te-enriched isotope spike Anal. Chim. Acta (IF 6.2) Pub Date : 2024-03-06 Tohru Fukazawa, Hajime Obata, Shiro Matsuoka, Satoshi Usui, Kazuhiro Norisuye
We present a method for the determination of ultra-trace Te species (Te(IV) and Te(VI)) in open ocean waters. The proposed method is based on Mg(OH) coprecipitation, anion exchange resin column separation and inductively coupled plasma sector-field mass spectrometry (ICPSFMS) using a Te-enriched isotope spike. The largest advantage of the method is that the use of the spike allows accurate and precise
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Differential detection of H1N1 virus spiker proteins by two hexaphenylbutadiene isomers based on size-matching principle Anal. Chim. Acta (IF 6.2) Pub Date : 2024-03-05 Xiaoling Pan, Jian Wang, Kai Zhang, Peng Sun, Jianbing Shi, Junge Zhi, Zhengxu Cai, Zi Li, Dayan Wang, Bin Tong, Yuping Dong
As one of the high pathogenic influenza viruses, H1N1 virus easily induces to serious diseases, even leading to death. To date, all detection methods for H1N1 virus had shortcomings, including high equipment cost, time consumption, and etc. Therefore, a novel detection method should be established to achieve more convenient, rapid, and low-cost detection. In this work, an isomer of HPBmN-I with aggregation-induced
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Ultrasensitive detection of zearalenone based on electrochemiluminescent immunoassay with Zr-MOF nanoplates and Au@MoS2 nanoflowers Anal. Chim. Acta (IF 6.2) Pub Date : 2024-03-05 Lu Peng, Xinyue Qian, Ya Jin, Xiangyang Miao, Anping Deng, Jianguo Li
In this work, an effective competitive-type electrochemiluminescence (ECL) immunosensor was constructed for zearalenone determination by using Zr-MOF nanoplates as the ECL luminophore and Au@MoS nanoflowers as the substrate material. Zr-MOF have an ultra-thin sheet-like structure that accelerates the transfer of electrons, ions and co-reactant intermediates, which exhibited strong and stable anodic
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Flexible microfluidic colorimetric detection chip integrated with ABTS·+ and Co@MnO2 nanozyme catalyzed TMB reaction systems for bio-enzyme free detection of sweat uric acid Anal. Chim. Acta (IF 6.2) Pub Date : 2024-03-05 Fang Li, Jianming Jiang, Nuotong Shen, Hao Peng, Yi Luo, Nannan Li, Liyang Huang, Yuyang Lu, Lifu Liu, Bing Li, Jianbo He
The development of wearable detection devices that can achieve noninvasive, on-site and real-time monitoring of sweat metabolites is of great demand and practical significance for point-of-care testing and healthcare monitoring. Monitoring uric acid (UA) content in sweat provides a simple and promising way to reduce the risk of gout and hyperuricemia. Traditional bioenzyme based UA assays suffer from
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Exploring the potential applications of amphiphilic carbon dots based nanocomposite hydrogel in liquid chromatographic separations Anal. Chim. Acta (IF 6.2) Pub Date : 2024-03-04 Qiaoling Liu, Kunming Zhou, Yanjuan Liu, Yuefei Zhang, Wei Chen, Sheng Tang
Due to their excellent stability, low toxicity, flexible modification and adjustable functionality, carbon dots (CDs) have a promising application prospect in the field of chromatographic stationary phases. Hydrogels are new functional polymer materials with three-dimensional network structure that have excellent hydrophilicity, high porosity and unique mechanical properties, which are also good candidate
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Enhanced split-type photoelectrochemical aptasensor incorporating a robust antifouling coating derived from four-armed polyethylene glycol Anal. Chim. Acta (IF 6.2) Pub Date : 2024-03-04 Hao Wang, Wei Li, Pengcheng Ni, Gao-Chao Fan, Xiliang Luo
Antifouling biosensors capable of preventing protein nonspecific adhesion in real human bodily fluids are highly sought-after for precise disease diagnosis and treatment. In this context, an enhanced split-type photoelectrochemical (PEC) aptasensor was developed incorporating a four-armed polyethylene glycol (4A-PEG) to construct a robust antifouling coating, enabling accurate and sensitive bioanalysis
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A SERS nanocellulose-paper-based analytical device for ultrasensitive detection of Alzheimer's disease Anal. Chim. Acta (IF 6.2) Pub Date : 2024-03-04 Wenwen Yuan, Hang Yuan, Ruibing Li, Ruiqi Yong, Ivona Mitrovic, Eng Gee Lim, Sixuan Duan, Pengfei Song
Alzheimer's disease (AD), the most prevalent neurodegenerative ailment worldwide, results in severe cognitive decline and irreversible memory loss. Early identification of AD is significant to patients for personalized intervention since effective cure and treatment methods for AD are lacking. Despite the severity of the disease, existing highly sensitive AD detection methods, including neuroimaging
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Oriented docking of the template for improved imprinting efficiency toward peptide with modifications Anal. Chim. Acta (IF 6.2) Pub Date : 2024-03-04 Juntao Zhou, Muhammad Mujahid Ali, Wenjing Yu, Xianhui Cheng, Yujun Gao, Lianghai Hu
Molecular imprinting polymers (MIPs) are synthetic receptors as biomimetic materials for various applications ranging from sensing to separation and catalysis. However, currently existing MIPs are stuck to some of the issues including the longer preparation steps and poor performance. In this report, a facile and one-pot strategy by integrating the in-situ growth of magnetic nanoparticles and reversed
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Derivatization of carboxylic groups prior to their LC analysis – A review Anal. Chim. Acta (IF 6.2) Pub Date : 2024-03-04 Timotej Jankech, Ivana Gerhardtova, Petra Majerova, Juraj Piestansky, Josef Jampilek, Andrej Kovac
Carboxylic acids (CAs) represent a large group of important molecules participating in various biologically significant processes. Analytical study of these compounds is typically performed by liquid chromatography (LC) combined with various types of detection. However, their analysis is often accompanied by a wide variety of problems depending on used separation system or detection method. The dominant
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A novel premixing strategy for highly sensitive detection of nitrite on paper-based analytical devices Anal. Chim. Acta (IF 6.2) Pub Date : 2024-03-03 Xiaohu Xu, Azadeh Nilghaz, Xiaofang Wan, Shan Liu, Meigui Xue, Wan Guo, Junfei Tian
Nitrite has been involved in many food processing techniques and its excessive consumption is closely related to the development of different diseases. Therefore, highly sensitive detection of nitrite is significant to ensure food safety. This study presents a simple and novel strategy for the highly sensitive detection of nitrite in food using paper-based analytical devices (PADs). In this proposed
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Addressing the untargeted lipidomics challenge in urine samples: Comparative study of extraction methods by UHPLC-ESI-QTOF-MS Anal. Chim. Acta (IF 6.2) Pub Date : 2024-03-02 Belen Fernandez Requena, Carolina Gonzalez-Riano, Coral Barbas
Urine analysis has remained a fundamental and widely used method in clinical diagnostics for over a century. With its minimal invasive nature and comprehensive range of analytes, urine has established itself as a clinical diagnostic tool for various disorders, including renal, urological, metabolic, and endocrine diseases. Furthermore, urine's unique attributes make it an attractive matrix for biomarker
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A wearable flexible electrochemical biosensor with CuNi-MOF@rGO modification for simultaneous detection of uric acid and dopamine in sweat Anal. Chim. Acta (IF 6.2) Pub Date : 2024-03-01 Cuncun Wang, Yong Zhang, Yiyi Liu, Xin Zeng, Changpeng Jin, Danqun Huo, Jingzhou Hou, Changjun Hou
In health assessment and personalized medical services, accurate detection of biological markers such as dopamine (DA) and uric acid (UA) in sweat is crucial for providing valuable physiological information. However, there are challenges in detecting sweat biomarkers due to their low concentrations, variations in sweat yield among individuals, and the need for efficient sweat collection. We synthesized
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Portable self-powered electrochemical aptasensing platform for ratiometric detection of mycotoxins based on multichannel photofuel cell Anal. Chim. Acta (IF 6.2) Pub Date : 2024-03-01 Kai Yan, Yifan Ding, Xuqiao Liu, Jianqiao Liu, Jingdong Zhang
Self-powered electrochemical sensors based on photofuel cells have attracted considerable research interest because their unique advantage of not requiring an external electric source, but their application in portable and multiplexed targets assay is limited by the inherent mechanism. In this work, a portable self-powered sensor constructed with multichannel photofuel cells was developed for the ratiometric
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First electrochemical investigation and determination of non-steroidal anti-inflammatory drug etofenamate using disposable pencil graphite electrode with voltammetric techniques Anal. Chim. Acta (IF 6.2) Pub Date : 2024-03-01 Aysel Yurdem, Mehmet Aslan, Hayriye Aral, Abdulkadir Levent
In this study, the electrochemical properties of etofenamate, an active ingredient belonging to the non-steroidal anti-inflammatory drug group, were investigated using cyclic voltammetry (CV) and square wave voltammetry (SW) techniques on a disposable pencil graphite electrode (PGE). With the CV technique, reversible voltammetric waves of around +0.470 V and irreversible voltammetric waves of around
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Biosensing-based quality control monitoring of the higher-order structures of therapeutic antibody domains Anal. Chim. Acta (IF 6.2) Pub Date : 2024-02-29 Hideki Watanabe, Naoko Hayashida, Megumi Sato, Shinya Honda
Advanced biopharmaceutical manufacturing requires novel process analytical technologies for the rapid and sensitive assessment of the higher-order structures of therapeutic proteins. However, conventional physicochemical analyses of denatured proteins have limitations in terms of sensitivity, throughput, analytical resolution, and real-time monitoring capacity. Although probe-based sensing can overcome
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Colorimetric and fluorescent independent dual “signal on” biosensor for accurate detection of ochratoxin A based on aptamer-triggered biocatalytic reactions Anal. Chim. Acta (IF 6.2) Pub Date : 2024-02-29 Hao Ding, Mingdi Zhang, Xiaochun Wang, Shuai He, Xiaokun Wang, Lingxin Chen
Ochratoxin A (OTA) is a hazardous food contaminant with significant health risks. Dual-channel OTA detection is noted for its cross-reference capability and high accuracy. Still, challenges in addressing in-system corrections and “signal off” related false positives and limited signal gains remain. Herein, we developed a dual-channel “signal on” aptasensor with one recognition process and two independent
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Development of a water-soluble fluorescent Al3+ probe based on phenylsulfonyl-2-pyrone in biological systems Anal. Chim. Acta (IF 6.2) Pub Date : 2024-02-29 Fumiko Hara, Naoko Mizuyama, Takeshi Fujino, Ashok Kumar Shrestha, Thenuwara Arachchige Omila Kasun Meetiyagoda, Shinya Takada, Hideo Saji, Takahiro Mukai, Masayori Hagimori
Al exists naturally in the environment and is an important component in acidic soils, which harm almost all plants. Furthermore, Al is widely used in food additives, cosmetics, and medicines, resulting in living organisms ingesting traces of Al orally or dermally every day. Accordingly, Al accumulates in the body, which can cause negative bioeffects and diseases, and this concern is gaining increasing
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A multifunctional fluorescent probe for sequential detection of hydrogen sulfide and pH in foodstuffs, living cells and mice Anal. Chim. Acta (IF 6.2) Pub Date : 2024-02-28 Zu-Sheng Huang, Wenxuan Zhang, Manshan Liang, Shihua Wang, Zhongda Zhang, Yongsheng Jiang, Xiaoxia Ye, Longteng Xie, Yun-Yun Quan
Cancer as a leading cause of premature death worldwide has become a major threat to human health due to the high incidence and mortality. Monitoring tumor markers are reliable and significantly important for early detection of cancers. In complex biological systems, it is of great urgency but still remains challenging to conceive a fluorescent probe with multiple tumor markers detection property. Hydrogen
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A review of the recent achievements and future trends on 3D printed microfluidic devices for bioanalytical applications Anal. Chim. Acta (IF 6.2) Pub Date : 2024-02-28 Lucas C. Duarte, Federico Figueredo, Cyro L.S. Chagas, Eduardo Cortón, Wendell K.T. Coltro
3D printing has revolutionized the manufacturing process of microanalytical devices by enabling the automated production of customized objects. This technology promises to become a fundamental tool, accelerating investigations in critical areas of health, food, and environmental sciences. This microfabrication technology can be easily disseminated among users to produce further and provide analytical
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Review: 3D cell models for organ-on-a-chip applications Anal. Chim. Acta (IF 6.2) Pub Date : 2024-02-28 Agnieszka Żuchowska, Patrycja Baranowska, Magdalena Flont, Zbigniew Brzózka, Elżbieta Jastrzębska
Two-dimensional (2D) cultures do not fully reflect the human organs' physiology and the real effectiveness of the used therapy. Therefore, three-dimensional (3D) models are increasingly used in bioanalytical science. Organ-on-a-chip systems are used to obtain cellular models, better reflecting the human body's characteristics and allowing us to obtain more reliable results than standard preclinical
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Ion transfer mediated by TEMPO in ionophore-doped thin films for multi-ion sensing by cyclic voltammetry Anal. Chim. Acta (IF 6.2) Pub Date : 2024-02-28 Gabriel J. Mattos, Justine A. Rothen, Nikolai Yu Tiuftiakov, Eric Bakker
We report here on the development of thin-layer ion-selective membranes containing lipophilic TEMPO as a phase-transfer redox mediator for the simultaneous detection of non-redoxactive ions. This redox probe was recently introduced by our group and provides ideal ion-transfer waves when the membrane is interrogated by cyclic voltammetry. To perform multianalyte detection in the same sensing film, plasticized
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UiO-66-NH2 initiated cascade reaction: Constructing a ratiometric fluorescence sensor for ultrasensitive detection of nerve agent simulant Anal. Chim. Acta (IF 6.2) Pub Date : 2024-02-27 You Lei, Nan Gao, Pengcheng Huang, Fang-Ying Wu
Highly toxic organophosphorus nerve agents often exist in the form of gas in the environment and can damage human neuroregulatory system by inhibiting the activity of acetylcholinesterase (AChE). However, fluorescent probes based on small organic molecules bring a secondary burden to environment, and their sensitivity and specificity for sarin simulant diethyl chlorophosphate (DCP) detection are unsatisfactory
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Calibration transfer via filter learning Anal. Chim. Acta (IF 6.2) Pub Date : 2024-02-27 Zhonghao Xie, Xiaojing Chen, Jean-Michel Roger, Shujat Ali, Guangzao Huang, Wen Shi
Calibration transfer is an essential activity in analytical chemistry in order to avoid a complete recalibration. Currently, the most popular calibration transfer methods, such as piecewise direct standardization and dynamic orthogonal projection, require a certain amount of standard or reference samples to guarantee their effectiveness. To achieve higher efficiency, it is desirable to perform the
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A triggered DNA nanomachine with enzyme-free for the rapid detection of telomerase activity in a one-step method Anal. Chim. Acta (IF 6.2) Pub Date : 2024-02-27 Huajie Pang, Yanan Peng, Rui Zhang, Zhijun Gao, Xiangde Lai, Dongxia Li, Xuan Zhao, Yuanyuan Wang, Hua Pei, Bin Qiao, Yuxiang Ji, Qiang Wu
Telomerase is considered a biomarker for the early diagnosis and clinical treatment of cancer. The rapid and sensitive detection of telomerase activity is crucial to biological research, clinical diagnosis, and drug development. However, the main obstacles facing the current telomerase activity assay are the cumbersome and time-consuming procedure, the easy degradation of the telomerase RNA template
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Three-dimensional DNA biomimetic networks (B-3D Net)-based ratiometric fluorescence platform for cancer-related gene biosensing Anal. Chim. Acta (IF 6.2) Pub Date : 2024-02-27 Chao Yao, Guiqun Zhang, Hongling Tao, Yulong Li, Rong Hu, Yunhui Yang
Efficient detection of cancer-related nucleic acids is pivotal for early cancer diagnosis. This study introduces a target induced three-dimensional DNA biomimetic networks (B-3D Net)-based ratiometric fluorescence platform using manganese dioxide nanosheets (MnO NS)/o-phenylenediamine in combination with hybridization chain reaction to detect cancer-related genes (p53 gene). The incorporation of multiple
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Comparison of single and double pulse laser-induced breakdown spectroscopy for the detection of biomolecules tagged with photon-upconversion nanoparticles Anal. Chim. Acta (IF 6.2) Pub Date : 2024-02-27 Zdeněk Farka, Karolína Vytisková, Ekaterina Makhneva, Eva Zikmundová, Daniel Holub, Jakub Buday, David Prochazka, Karel Novotný, Petr Skládal, Pavel Pořízka, Jozef Kaiser
Laser-induced breakdown spectroscopy (LIBS) is a well-recognized analytical technique used for elemental analysis. This method is gaining considerable attention also in biological applications thanks to its ability for spatial mapping and elemental imaging. The implementation of LIBS in the biomedical field is based on the detection of metals or other elements that either naturally occur in the samples
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miRNA and DNA analysis by negative ion electron transfer dissociation and infrared multiple-photon dissociation mass spectrometry Anal. Chim. Acta (IF 6.2) Pub Date : 2024-02-27 Miriam Guzmán-Lorite, Frédéric Rosu, María Luisa Marina, María Concepción García, Valérie Gabelica
The use of simple and hybrid fragmentation techniques for the identification of molecules in tandem mass spectrometry provides different and complementary information on the structure of molecules. Nevertheless, these techniques have not been as widely explored for oligonucleotides as for peptides or proteins. The analysis of microRNAs (miRNAs) warrants special attention, given their regulatory role
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A novel fluorescent probe for visualizing viscosity changes in lipid droplets during chemotherapy-induced ferroptosis Anal. Chim. Acta (IF 6.2) Pub Date : 2024-02-27 Di Wei, Yingshu Dai, Jing Cao, Nanyan Fu
Ferroptosis, as a novel form of cell death, is becoming one of the hot topics in cancer treatment research. It differs from necrosis and autophagy in that it involves the accumulation of lipid peroxides and is triggered by iron dependency. Recent studies have suggested that this mechanism may alter the viscosity or structure of lipid droplets (LDs). The relationship between LDs viscosity and ferroptosis
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Triplex DNA-based aggregation-induced emission probe: A new platform for hybridization chain reaction-based fluorescence sensing assay Anal. Chim. Acta (IF 6.2) Pub Date : 2024-02-27 Yubin Li, Ciling Li, Chuangqiang Zhang, Liting Zhao, Yaoyun Huang
The hybridization chain reaction (HCR), as one of the nucleic acid amplification technologies, is combined with fluorescence signal output with excellent sensitivity, simplicity, and stability. However, current HCR-based fluorescence sensing methods still have some defects such as the blocking effect of the HCR combination with fluorophores and the aggregation-caused quenching (ACQ) phenomenon of traditional
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Magnetic photoelectrochemical sensor array utilizing addressing sensing strategy for simultaneous detection of amyloid-β 42 and microtubule-associated protein tau Anal. Chim. Acta (IF 6.2) Pub Date : 2024-02-27 Shuo Zhang, Yunfei Zhang, Huan Wang, Yaoguang Wang, Hongmin Ma, Dan Wu, Zhong Feng Gao, Dawei Fan, Xiang Ren, Qin Wei
The accurate diagnosis of diseases can be improved by detecting multiple biomarkers simultaneously. This study presents the development of a magnetic photoelectrochemical (PEC) immunosensor array for the simultaneous detection of amyloid-β 42 (Aβ) and microtubule-associated protein (Tau), which are markers for neurodegenerative disorders. A metal-organic framework (MOF) derivative, FeO@FeS magnetic
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Golgi apparatus-targeting fluorescent probe for the imaging of superoxide anion (O2•−) in living cells during ferroptosis Anal. Chim. Acta (IF 6.2) Pub Date : 2024-02-26 Jia Chang, Yan Wang, Xiuqi Kong, Baoli Dong, Tao Yue
Ferroptosis is an emerging iron-dependent oxidative cell death type, and recently has been demonstrated to show close relation with Golgi apparatus (GA). Exploring the fluctuation of superoxide anion (O) level in GA during ferroptosis is of great significance to profoundly study the biological functions of GA in ferroptosis. Here, we present a GA-targeting probe () to monitor cellular O during ferroptosis
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Streamlining regular liquid chromatography with MALDI-TOF MS and NMR spectroscopy using automatic full-contact splitless spotting interface and flash-tap fractioning collection Anal. Chim. Acta (IF 6.2) Pub Date : 2024-02-26 Chi-Minh Truong, Yung-Cheng Jair, Hong-Po Chen, Wei-Chih Chen, Yi-Hsin Liu, Pin-Chuan Chen, Pai-Shan Chen
High-resolution matrix-assisted laser desorption/ionization-time of flight mass spectrometry (MALDI-TOF MS) and nuclear magnetic resonance (NMR) spectroscopy are powerful tools to identify unknown psychoactive substances. However, in complex matrices, trace levels of unknown substances usually require additional fractionation and concentration. Specialized liquid chromatography systems are necessary
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A high-throughput DNA analysis method based on isothermal amplification on a suspension microarray for detecting mpox virus and viruses with comparable symptoms Anal. Chim. Acta (IF 6.2) Pub Date : 2024-02-26 Liming Zhang, Jieyu Liu, Shisi Huang, Wentao Zeng, Li Li, Xihao Fan, Zhuoxuan Lu
Mpox is a zoonotic disease caused by mpox virus (MPXV) infection. Since May 2022, there has been a marked increase in human mpox cases in different regions. Rash, fever, and sore throat are typical signs of mpox. However, other viruses, such as the B virus (BV), herpes simplex virus types 1 (HSV-1), herpes simplex virus types 2 (HSV-2), and varicella zoster virus (VZV), can also infect people and cause
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Fluorometric “AND” logic gate for detection of tyramine and tyrosinase based on in-situ formation of silicon-containing nanoparticles Anal. Chim. Acta (IF 6.2) Pub Date : 2024-02-26 Tingting Wang, Fangning Liu, Chuanxia Chen, Yizhong Lu
Tyramine is an important index of food freshness degree, and tyrosinase that can specifically oxidized monophenolamine to catecholamine plays a crucial part in the occurrence and development of melanin-related skin diseases. Therefore, it is crucial to develop sensitive and efficient methods for the detection of tyramine and tyrosinase. In this work, encouraged by tyrosinase-triggered specific oxidation
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Exploring T7 RNA polymerase-assisted CRISPR/Cas13a amplification for the detection of BNP via electrochemiluminescence sensing platform Anal. Chim. Acta (IF 6.2) Pub Date : 2024-02-26 Zaiyong Zhang, Jinglong Li, Chunlin Chen, Yuwei Tong, Dehui Liu, Cuizhi Li, Huan Lu, Li Huang, Wanling Feng, Xiaoting Sun
Brain natriuretic peptide (BNP) is considered to be an important biomarker of heart failure (HF) attracting attention. However, its low concentration and short half-life in blood lead to a low-sensitivity detection of BNP, which is a challenge that has to be overcome. In this work, we propose a highly specific, highly sensitive T7 RNA polymerase-assisted clustered regularly interspaced short palindromic
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Solvent-free parallel artificial liquid membrane extraction for drugs of abuse in plasma samples using LC-MS/MS Anal. Chim. Acta (IF 6.2) Pub Date : 2024-02-26 André Luis Fabris, Stig Pedersen-Bjergaard, Elisabeth Leere Øiestad, Giordano Novak Rossi, Jaime E.Cecílio Hallak, Rafael Guimarães dos Santos, Jose Luiz Costa, Mauricio Yonamine
Parallel artificial liquid membrane extraction (PALME) is a 96-well plate setup variant of liquid-phase microextraction. Basic or acidic analytes are extracted in neutral form from the sample, through a supported liquid membrane (SLM), and into aqueous acceptor. PALME is already considered a green extraction technique, but in the current conceptual work, we sought to make it even greener by replacing
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An in silico scheme for optimizing the enzymatic acquisition of natural biologically active peptides based on machine learning and virtual digestion Anal. Chim. Acta (IF 6.2) Pub Date : 2024-02-26 Like Lin, Cong Li, Tianlong Zhang, Chaoshuang Xia, Qiuhong Bai, Lihua Jin, Yehua Shen
As a potential natural active substance, natural biologically active peptides (NBAPs) are recently attracting increasing attention. The traditional proteolysis methods of obtaining effective NBAPs are considerably vexing, especially since multiple proteases can be used, which blocks the exploration of available NBAPs. Although the development of virtual digesting brings some degree of convenience,
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Novel method for chlorine determination in biofuel lipid feedstock by coupling a total sample introduction system (hTISIS) to an inductively coupled plasma tandem mass spectrometry (ICP-ms/MS) Anal. Chim. Acta (IF 6.2) Pub Date : 2024-02-26 Santiago Martínez, Loïs Morineau, Joana Fernandes, Fabien Chainet
Chlorine concentrations above 1 mg kg in lipid feedstocks for biofuel production can generate corrosion issues in the different refining units as well as catalyst deactivation by clogging or fouling. To reach accurate analyses by inductively coupled plasma (ICP) techniques at low concentration levels, dilution in organic solvents rises as a simpler and more straightforward sample preparation methodology
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Portable photoelectrochemical immunoassay with micro-electro-mechanical-system for alpha-fetoprotein in hepatocellular carcinoma Anal. Chim. Acta (IF 6.2) Pub Date : 2024-02-26 Fangming Lou, Shaojie Wang, Bo Han, Qunfang Li, Dianping Tang
Early detection of cancer has a profound impact on patient survival and treatment outcomes considering high treatment success rates and reduced treatment complexity. Here, we developed a portable photoelectrochemical (PEC) immune platform for sensitive testing of alpha-fetoprotein (AFP) based on Pt nanocluster (Pt NCs) loaded defective-state g-CN photon-electron transducers. The broad forbidden band
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Mg2+-promoted high-efficiency DNA conjugation on polydopamine surfaces for aptamer-based ochratoxin A detection Anal. Chim. Acta (IF 6.2) Pub Date : 2024-02-26 Wei Yang, Liang Feng
Surface immobilization of DNA is the foundation of a broad range of applications in biosensing and specific DNA extraction. Polydopamine (PDA) coatings can serve as intermediate layers to immobilize amino- or thiol-labelled molecules, including DNA, onto various materials through Michael addition and/or Schiff base reactions. However, the conjugation efficiency is limited by electrostatic repulsion
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Improvement of the selectivity of a molecularly imprinted polymer-based potentiometric sensor by using a specific functional monomer Anal. Chim. Acta (IF 6.2) Pub Date : 2024-02-25 Junhao Wang, Rongning Liang, Wei Qin
Potentiometric sensors based on the molecularly imprinted polymers (MIPs) as the receptors have been successfully developed for determination of various organic and biological species. However, these MIP receptors may suffer from problems of low selectivity. Especially, it would be difficult to distinguish the target analyte from its structurally similar interferents. In this work, we propose a novel
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Design of a dual-mode ratiometric fluorescent probe via MOF-on-MOF strategy for Al (III) and pH detection Anal. Chim. Acta (IF 6.2) Pub Date : 2024-02-24 Ruirui Zhao, Wenjing Lu, Xiaojing Chai, Chuan Dong, Shaomin Shuang, Yujing Guo
The construction of ratiometric fluorescent MOF sensors with integrated self-calibration and dual-channel detection can efficiently overcome the deficiencies of single-signal sensing. In this regard, the rational design of structurally functionalized MOFs is paramount for enhancing their performance in ratiometric fluorescent sensors. Lately, the concept of MOF-on-MOF design has garnered notable interest
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Copper-cobalt dual-site on N-doped carbon nanotube with dual-promoted synergy for glucose electrochemical detection Anal. Chim. Acta (IF 6.2) Pub Date : 2024-02-23 Zhenlu Zhao, Peihan Wang, Yizhong Lu
Doping specific active sites and accelerating the decisive step of glucose catalysis to construct highly active glucose sensing electrochemical catalysts remains a major challenge for glucose sensing. Herein, we report the detailed design of Cu–Co dual active site N-doped carbon nanotube (CuCo-NCNTs) obtained by electrodeposition modification, programmed warming and calcination for electrochemical
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GAA/(Au–Au/IrO2)@Cu(PABA) reactor with cascade catalytic activity for α-glucosidase inhibitor screening Anal. Chim. Acta (IF 6.2) Pub Date : 2024-02-23 Yingying Zhong, Qing Wang, Zi-Jian Chen, Hongwu Wang, Suqing Zhao
screening strategies based on the inhibition of α-glucosidase (GAA) activity have been widely used for the discovery of potential antidiabetic drugs, but they still face some challenges, such as poor enzyme stability, non-reusability and narrow range of applicability. To overcome these limitations, an screening method based on GAA@GOx@Cu-MOF reactor was developed in our previous study. However, the
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Controlled construction of 2D hierarchical core-shell ZnO/MnO2 nanosheets on Nitinol fiber with enhanced adsorption performance for selective solid-phase microextraction of trace polycyclic aromatic hydrocarbons in water samples Anal. Chim. Acta (IF 6.2) Pub Date : 2024-02-22 Hua Zhou, Jiayu Li, Huirong Li, Haixia Liu, Xuemei Wang, Xinzhen Du
Polycyclic aromatic hydrocarbons (PAHs) are an important class of potentially toxic persistent organic pollutants in environmental water. Their concentrations are usually too low to allow for direct determination with analytical instruments, and the preconcentration is required prior to instrumental analysis. Solid phase microextraction (SPME) is considered as a high-performance green sample preparation
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Highly efficient dual-mode detection of AFB1 based on the inner filter effect: Donor-acceptor selection and application Anal. Chim. Acta (IF 6.2) Pub Date : 2024-02-22 Jincheng Xiong, Boyan Sun, Shuai Zhang, Sihan Wang, Linqian Qin, Haiyang Jiang
The utilization of inner filter effect (IFE) brings more opportunities for construction of fluorescence immunoassays but remains a great challenge, especially how to select best donor in the face of extensive fluorescent nanomaterials. Aflatoxin B1 possesses high toxicity among mycotoxins and is frequently found in agricultural products that may significantly threaten to human health. Therefore, with
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A microfluidic chip-based capillary zone electrophoresis-mass spectrometry method for measuring adenosine 5′-Triphosphate and its similar nucleotide analogues Anal. Chim. Acta (IF 6.2) Pub Date : 2024-02-21 Cristina Di Poto, Xiang Tian, Scott Mellors, Sanna Rosengren, Sabina Issop, Sara J. Bonvini, Sonja Hess, Erik L. Allman
Extracellular ATP is involved in disorders that cause inflammation of the airways and cough, thus limiting its release has therapeutic benefits. Standard luminescence-based ATP assays measure levels indirectly through enzyme degradation and do not provide a simultaneous readout for other nucleotide analogues. Conversely, mass spectrometry can provide direct ATP measurements, however, common RPLC and
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Construction of single-molecule counting-based biosensors for DNA-modifying enzymes: A review Anal. Chim. Acta (IF 6.2) Pub Date : 2024-02-21 Qian Zhang, Juan Hu, Dong-ling Li, Jian-Ge Qiu, Bing-Hua Jiang, Chun-yang Zhang
DNA-modifying enzymes act as critical regulators in a wide range of genetic functions (e.g., DNA damage & repair, DNA replication), and their aberrant expression may interfere with regular genetic functions and induce various malignant diseases including cancers. DNA-modifying enzymes have emerged as the potential biomarkers in early diagnosis of diseases and new therapeutic targets in genomic research