The increasing manufacture of plastics and their mismanagement has turned plastic into a ubiquitous waste in the marine environment. Among all the substances conforming the plastic items, the effects of a dietary Bisphenol A (BPA) and Di-isononyl phthalate (DiNP) have been evaluated in adult male gilthead sea bream, focusing on their effects in the modulation of the Endocannabinoid System (ECS). In zebrafish, the ECS has been recently chosen as a new target for the activity of some Endocrine Disrupting Chemicals (EDC), since it represents a complex lipid signaling network essential for the well-being of the organisms. The results obtained in gilthead seabream showed that BPA and DiNP altered the structure and the biochemical composition of liver, increasing the presence of lipids and triglycerides and decreasing the glycogen and phospholipids. Moreover, the addition of BPA or DiNP in the gilthead sea bream diet altered the levels of endocannabinoids (EC) and EC-like mediators in the liver. These alterations were also associated to changes at the transcriptomic level of genes involved in lipid biosynthesis and ECS metabolism. At the central level, both BPA and DiNP reduced the expression of the endocannabinoid receptor type I (cnr1) and the neuropeptide Y (npy) as well as the levels of the endocannabinoid Anandamide (AEA), suggesting a downregulation of appetite. The results herein reported highlighted the negative effects of chronic dietary exposure to DiNP or BPA on ECS functions and lipid metabolism of male gilthead sea bream liver, showing a similar disruptive activity of these contaminants at metabolic level. Moreover, the novelty of the biomarkers used evidenced possible innovative endpoints for the development of novel OEDCS test guidelines.

塑料生产的增加及其管理不当，已使塑料成为海洋环境中无处不在的废物。在所有符合塑料物品的物质中，饮食双酚A（BPA）和邻苯二甲酸二异壬酯（DiNP）的作用已在成​​年雄性金头鲷中进行了评估，重点在于它们在调节内源性大麻素系统（ECS）中的作用）。在斑马鱼中，ECS最近被选作某些内分泌干扰化学物质（EDC）活性的新靶标，因为它代表着复杂的脂质信号网络对于生物的福祉至关重要。在银头鲷中获得的结果表明，BPA和DiNP改变了肝脏的结构和生化组成，增加了脂质和甘油三酸酯的存在，并降低了糖原和磷脂。此外，在金头鲷的饮食中添加BPA或DiNP会改变肝脏中内源性大麻素（EC）和类EC介质的水平。这些改变也与参与脂质生物合成和ECS代谢的基因在转录水平上的改变有关。在中央水平，BPA和DiNP均降低了I型内源性大麻素受体（cnr1）和神经肽Y（npy）以及内源性大麻素Anandamide（AEA）的水平，表明食欲下调。本文报道的结果突显了长期饮食中DiNP或BPA暴露对雄性金头鲷鱼肝的ECS功能和脂质代谢的负面影响，显示了这些污染物在代谢水平上具有类似的破坏活性。此外，所使用的生物标记物的新颖性为开发新型OEDCS测试指南提供了可能的创新终点。