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  • Bioavailability of Quercetin from Onion Extracts after Intraruminal Application in Cows
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-25
    Silvia Wein, Birgit Beyer, Benno F. Zimmermann, Ralf H. Blank, Siegfried Wolffram
    更新日期:2018-09-25
  • Sensitive Screening Method for Determination of Pyrethroids in Chicken Eggs and Various Meat Samples by Gas Chromatography and Electron Capture Detection
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-25
    Valeria Nardelli, Francesco Casamassima, Giuseppe Gesualdo, Donghao Li, Wadir M. V. Marchesiello, Donatella Nardiello, Maurizio Quinto
    更新日期:2018-09-25
  • The effect of Lactobacillus plantarum NCU116 fermentation on Asparagus officinalis polysaccharide: Characterization, antioxidative, and immunoregulatory activities
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-25
    Zhi-Hong Zhang, Songtao Fan, Dan-Fei Huang, Qiang Yu, Xiao-Zhen Liu, Chang Li, Sunan Wang, Tao Xiong, Shao-Ping Nie, Ming-Yong Xie

    Lactic acid fermentation represents novel method to produce bioactive functional ingredients, including polysaccharides. In this work, a selected lactic acid bacteria strain NCU116 was used to ferment Asparagus officinalis (asparagus) pulps. Two polysaccharides were subsequently separated from both unprocessed and fermented asparagus pulps, namely asparagus polysaccharide (AOP) and fermented-AOP (F-AOP). The physicochemical and bioactive properties of AOP and F-AOP were characterized and investigated. High-performance anion-exchange chromatography showed that fermentation increased the proportions of rhamnose, galacturonic acid, and glucuronic acid in polysaccharides by 46.70, 114.09 and 12.75‰, respectively. High-performance size-exclusion chromatography revealed that fermentation decreased the average molecular weight from 181.3 kDa (AOP) to 152.8 kDa (F-AOP). Moreover, the fermentation reduced the particle size and changed the rheology property. In vitro, F-AOP displayed superior free radical scavenging properties than AOP, using DPPH, hydroxyl and superoxide anion radical scavenging assays. In vivo, F-AOP administration dose-dependently promoted a gradual shift from Th17-dominant acute inflammatory response (IL-17 and RORγt) to Th1-dominant defensive immune response (IFN-γ and T-bet). These results indicated that the lactobacillus plantarum NCU116 fermentation was practical and useful to obtain promising bioactive polysaccharides.

    更新日期:2018-09-25
  • Structural and Biochemical Characterization of the Curcumin-Reducing Activity of CurA from Vibrio vulnificus
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-25
    Soo-Bong Park, Da-Woon Bae, Nina Abigail Bagay Clavio, Lei Zhao, Chang-Sook Jeong, Bomi Choi, Stephani Joy Yarcia Macalino, Hee-Jeong Cha, Jin-Byung Park, Jun Hyuck Lee, Sang-Jip Nam, Sun Choi, Min-Kyu Kim, Sun-Shin Cha

    Curcumin is a yellow-colored ingredient in dietary spice turmeric (Curcuma longa Linn). This nontoxic polyphenol has anti-tumor, anti-inflammatory, apoptotic, and anti-oxidant activities. The ingested curcumin is reduced to multihydrated forms with more potent therapeutic potentials by the curcumin reductase (CurA) from commensal Escherichia coli. In this study, we demonstrated that Vibrio vulnificus CurA (VvCurA) with 87% sequence similarity to the E. coli CurA exhibits the curcumin-reducing activity through spectrophotometric detection of NADPH oxidation and high performance liquid chromatographic analysis of curcumin consumption and product generation. Afterward, we determined the crystal structures of VvCurA and the VvCurA/NADPH complex, and made the in silico model of the VvCurA/NADPH/curcumin ternary complex through induced fit docking. Based on structural information, active site residues that play critical roles in catalysis have been identified and characterized by mutational and kinetic studies, leading us to propose the reaction mechanism of CurA.

    更新日期:2018-09-25
  • Generic haptens synthesis, broad-specificity monoclonal antibodies preparation and ultrasensitive ELISA for five antibacterial synergists in chicken and milk
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-25
    Hongfang Li, Shaoqin Ma, Xiya Zhang, Chenglong Li, Baolei Dong, Mari Ghulam Mujtaba, Yujie Wei, Xiao Liang, Xuezhi Yu, Kai Wen, Wenbo Yu, Jianzhong Shen, Zhanhui Wang

    An antibody with broad-specificity and principally depending on hapten structure and size is a key reagent for developing a class-selective immunoassay. In the present study, three new generic haptens of antibacterial synergists (ASGs) were proposed using trimethoprim as the start molecule. These haptens contained carboxyl groups on the meta position of trimethoxybenzene for conjugating to protein, while, the common moiety of ASGs, i.e., diaminopyrimidine, was intentionally and maximally exposed to the immune system in animals in order to induce antibodies with broad-specificity against ASGs. Five monoclonal antibodies (mAbs) were finally obtained, and 5C4 from the hapten with a short spacer arm, named Hapten A, showed not only uniform broad specificity but also high affinity to all five ASGs. We further determined the possible recognition mechanism of mAbs in terms of conformational and electronic aspects. A indirect competitive ELISA (icELISA) based-5C4 was established and exhibited IC50 values of 0.067–0.139 μg L−1 with cross-reactivity of 48.2%–418.7% for the five ASGs in buffer under optimal conditions. The calculated limits of detection of the icELISA for chicken and milk were 0.06 μg kg−1–0.8 μg kg−1 and 0.05 μg L−1–0.6 μg L−1, respectively. The recoveries in spiked chicken and milk samples were 75.2%–101.4% with a coefficient of variation less than 14.3%. In conclusion, we developed, for the first time, a rapid and reliable icELISA for ASGs with significantly improved sensitivity and class-selectivity.

    更新日期:2018-09-25
  • Formation of Whey Protein Isolate (WPI)–Maltodextrin Conjugates in Fibers Produced by Needleless Electrospinning
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-24
    Ines Kutzli, Monika Gibis, Stefan K. Baier, Jochen Weiss
    更新日期:2018-09-25
  • 更新日期:2018-09-25
  • Enhancement of the Production of Bioactive Microalgal Metabolites by Ultraviolet Radiation (UVA 365 nm)
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-24
    Jim Junhui Huang, Shaoling Lin, Wenwen Xu, Peter Chi Keung Cheung
    更新日期:2018-09-25
  • 更新日期:2018-09-25
  • Species Differentiation and Quantification of Processed Animal Proteins and Blood Products in Fish Feed Using an 8-Plex Mass Spectrometry-Based Immunoassay
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-24
    Andreas E. Steinhilber, Felix F. Schmidt, Wael Naboulsi, Hannes Planatscher, Alicia Niedzwiecka, Jutta Zagon, Albert Braeuning, Alfonso Lampen, Thomas O. Joos, Oliver Poetz
    更新日期:2018-09-25
  • Method Development and Validation of Indaziflam and Its Five Metabolites in Soil, Water, and Fruits by Modified QuEChERS and UHPLC-MS/MS
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-24
    Mingfeng Hu, Jingsi Qiu, Hui Zhang, Xiaosu Fan, Kunfeng Liu, Dongqiang Zeng, Huihua Tan
    更新日期:2018-09-25
  • Graphene Oxide Quantum Dots Assisted Construction of Fluorescent Aptasensor for Rapid Detection of Pseudomonas Aeruginosa in Food Samples
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-24
    Ran Gao, Zitao Zhong, Xiaomei Gao, Li Jia

    We engineered an ingenious fluorescent aptasensor for detection of Pseudomonas aeruginosa (P. aeruginosa) according to the DNA hybridization and the fluorescence resonance energy transfer. In the absence of target bacteria, 5-carboxyfluorescein-labelled complementary DNA (FAM-cDNA) hybridizes with the partial sequences of aptamer and the fluorescence of FAM can be quenched by graphene oxide quantum dots (GOQDs). Upon the addition of target bacteria, the aptamer as a biorecognition element is bound with P. aeruginosa specifically. FAM-cDNA prefers to hybridize with the aptamer, resulting in the desorption of FAM-cDNA from GOQDs, thus recovering the fluorescence of FAM. The aptasensor shows a wide linear response to P. aeruginosa in the concentration range of 1.28 × 103 ~ 2.00 × 107 cfu/mL with acceptable selectivity. The detection limit is 100 cfu/mL. The whole process can be finished in 2 hours. Moreover, the platform is successfully applied to detect P. aeruginosa in drinking water, orange juice, as well as popsicle samples.

    更新日期:2018-09-25
  • Heating-aided pH Shifting Modifies Hemp Seed Protein Structure, Cross-linking, and Emulsifying Properties
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-24
    Qingling Wang, Yan Jin, Youling L. Xiong

    Alkaline pH12 shift treatment performed at varying temperatures (20 to 80 °C for 1, 5, and 60 min) was applied to structurally modify hemp seed protein isolate (HPI). The solubility of HPI (~20%) was remarkably improved (P ˂ 0.05) with elevating temperature and prolonging holding time, reaching 97.5% at 80 °C for 60 min. The treated HPI exhibited a strong tendency of forming soluble large aggregates. To limit lysinoalanine (LAL) production, heating was methodically controlled to 60 °C and 5 min where the LAL content never exceeded 100 mg/100 g protein and the loss of cysteine and lysine was also minimal. The emulsifying activity of HPI was improved by this mild pH shift–heating combination treatment due to the dissociation of protein subunits, unraveling of tertiary structure, and exposure of hydrophobic groups. Moreover, the emulsion formed by the treated protein maintained a superior stability in particle size and distribution during storage.

    更新日期:2018-09-25
  • Chemistry of human breast milk – a comprehensive review of the composition and role of milk metabolites in child development
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-24
    Dorota Garwolińska, Jacek Namieśnik, Agata Kot-Wasik, Weronika Hewelt-Belka

    Early nutrition has an enormous influence on a child's physiological function, immune system maturation and cognitive development. Human breast milk (HBM) is recognized as the gold standard for human infant nutrition. Accordingly to WHO report breastfeeding is consider as an unequalled way of providing ideal food to the infant, which is required for his healthy growth and development. HBM contains various macronutrients (carbohydrates, proteins, lipids, and vitamins) as well as numerous bioactive compounds and interactive elements (growth factors, hormones, cytokines, chemokines, and antimicrobial compounds. The aim of this review is to summarize and discuss the current knowledge about metabolites, which are the least understood components of HBM, and their potential role in infant development. We focus on small metabolites (<1500 Da) and characterize the chemical structure and biological function of polar metabolites such as human milk oligosaccharides, nonprotein molecules containing nitrogen (creatine, amino acids, nucleotides, polyamines) and nonpolar lipids. We believe that this manuscript will provide a comprehensive insight into a HBM metabolite composition, chemical structure and their role in a child’s early-life nutrition.

    更新日期:2018-09-25
  • Lipid-lowering effects of medium-chain triglyceride-enriched coconut oil in combination with licorice extracts in experimental hyperlipidemic mice
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-24
    Eun-Jung Lee, Hyeongjoo Oh, Beom Goo Kang, Min-Kyung Kang, Dong Yeon Kim, Yun-Ho Kim, Jeong Yeol Lee, Joung Gun Ji, Soon Sung Lim, Young-Hee Kang

    Coconut oil has gained in popularity over recent years as healthy oil due to its potential cardiovascular benefits. Coconut oil contains medium chain triglycerides (MCT) including lauric acid and capric acid that display beneficial properties in human health. Licorice (Glycyrrhiza uralensis) is used as a sweetener and in traditional Chinese medicine with anti-inflammatory, anti-microbial and antioxidant activities. This study investigated the in vivo effects of medium chain-triglycerides (MCT)-coconut oil (MCO) and its combination with licorice extract (LE-MCO) on serum lipid profile, hepatic steatosis, and local fat pad proteins in diet-induced obese mice. No liver toxicity was observed in 45% fat diet (HFD)-fed mice orally treated with LE, MCO and LE-MCO for 12 weeks. Their supplementation reduced HFD-enhanced body weight, blood glucose and insulin in mice. Plasma levels of both PLTP and LCAT were boosted in LE-MCO-administrated mice. Supplementation of LE-MCO diminished plasma levels of TG and TC with concomitant reduction of the LDL-C level and tended to raise blood HDL-C level, compared to that of HFD alone-mice. Treatment of LE-MCO encumbered the hepatic induction of hepatosteatosis-related proteins of SREBP2, SREBP1c FAS, ACC and CD36 in HFD-fed mice. 6) Substantial suppression of this induction was also observed in the liver of mice treated with MCO. Oral administration of LE-MCO to HFD mice boosted hepatic activation of AMPK and the induction of UCP-1 and FATP1 in brown fat. Conversely, LE-MCO disturbed hepatic PPAR-LXR-RXR signaling in HFD-fed animals and reversed HFD-elevated epididymal PPARγ. Collectively, oral administration of LE-MCO may impede hyperlipidemia and hepatosteatosis through curtailing hepatic lipid synthesis.

    更新日期:2018-09-25
  • Black Sesame Seeds Ethanol Extract Ameliorates Hepatic Lipid Accumulation, Oxidative Stress and Insulin Resistance in Fructose-induced Nonalcoholic Fatty Liver Disease
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-24
    Yang Yang, Jingjing Wang, Yong-min Zhang, Jing Li, Wenji Sun

    The aim of the present study was to investigate the effect of black sesame seeds ethanol extract (BSSEE) against nonalcoholic fatty liver disease (NAFLD) in fructose-fed mice. Mice were fed a standard diet without or with 30% fructose in drinking water for 8 consecutive weeks, while mice in three BSSEE tested groups received different doses of BSSEE (0.5, 1 and 2 mL/kg) once a day from the 5th week to the 8th week. Administration of BSSEE dose-dependently exerted antiobesity and protective effect against metabolism disorder in fructose-fed mice. Histological examinations indicated that administration of BSSEE obviously reduced hepatic lipid accumulation. Insulin tolerance test (ITT) and glucose tolerance test (GTT) along with decreases of serum insulin and glucose levels by BSSEE treatment suggested the improvement of body insulin resistance, and administration of 1 and 2 mL/kg BSSEE mitigated liver insulin resistance as the evidences of down-regulated expression of phospho-JNK1/2/3, phospho-NF-κB p65, phospho-IRS1 and phospho-IKK alpha/beta, up-regulated XBP1 expression and reductions of TNF-α and IL-6 levels. In addition, BSSEE treatment ameliorated hepatic oxidative stress through increasing GSH, vitamin C and Nrf2 levels, decreasing MDA and NO levels, and enhancing SOD, CAT and GSH-Px activities. These results demonstrated that BSSEE showed protective effects against NAFLD-related metabolic diseases in fructose-fed mice. Therefore, BSSEE may be a potent dietary supplement to ameliorate the diseases.

    更新日期:2018-09-25
  • Metabolic Engineering of Four GATA Factors to Reduce the Urea and Ethyl Carbamate Formation in a Model Rice Wine System
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-24
    Peng Zhang, Bang Li, Peng Wen, Peilin Wang, Yu Yang, Qian Chen, Yuxin Chang, Xing Hu

    Urea is the most important precursor of ethyl carbamate (EC), a harmful carcinogenic product, in fermented wines. In this study, the effects of four GATA transcriptional factors (Gln3p, Gat1p, Dal80p and Gzf3p) on extracellular urea and EC formation and transcriptional changes in urea degradation related genes (DUR1,2 and DUR3) were examined. Compared to the WT strain, the Δgzf3 mutant showed 18.7% urea reduction, and exhibited synergistic effects with overexpressed Gln3p1-653 and Gat1p1-375 on extracellular urea reduction. Moreover, Δgzf3+Gln3p1-653 and Δgzf3+Gat1p1-375 showed a significant 38.7% and 43.7% decreases in the urea concentration, and 41.7% and 48.5% decreases in the EC concentration, respectively, in a model rice wine system. These results provide a promising way to reduce urea and EC formation during wine fermentation and raise some cues for the regulations of the four GATA transcriptional factors on the expression of individual nitrogen catabolite repression sensitive genes and their related metabolism pathway.

    更新日期:2018-09-25
  • Aroma Compounds in Chicken Broths of Beijing Youji and Commercial Broilers
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-21
    Mengdie Fan, Qunfei Xiao, Jianchun Xie, Jie Cheng, Baoguo Sun, Wenbin Du, Yaxin Wang, Tianze Wang
    更新日期:2018-09-22
  • Eriodictyol Attenuates LPS-Induced Neuroinflammation, Amyloidogenesis, and Cognitive Impairments via the Inhibition of NF-κB in Male C57BL/6J Mice and BV2 Microglial Cells
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-21
    Pandi He, Shikai Yan, Jiaojiao Zheng, Yuxing Gao, Shuhan Zhang, Zhigang Liu, Xuebo Liu, Chunxia Xiao
    更新日期:2018-09-22
  • Oral administration of salecan-based hydrogels for controlled insulin delivery
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-21
    Xiaoliang Qi, Yue Yuan, Jianfa Zhang, Jeff W. M. Bulte, Wei Dong

    We present an improved type of food gum (salecan) based hydrogels for oral delivery of insulin. Structural hydrogel formation was assessed with Fourier transform infrared spectroscopy, thermogravimetric analysis and X-ray diffraction. We found that the hydrogel modulus, morphology, and swelling properties can be controlled by varying the salecan dose during hydrogel formation. Insulin was introduced into the hydrogel using a swelling–diffusion approach and then further used a drug prototype. In vitro insulin release profiles demonstrated that the release of entrapped insulin was suppressed in acidic conditions, but markedly increased at neutral pH. Cell viability and toxicity tests revealed that the salecan hydrogel constructs were biocompatible. Oral administration of insulin-loaded salecan hydrogels in diabetic rats resulted in a sustained decrease of fasting plasma glucose levels over 6 h post-administration. For non-diabetic animals, the relative pharmacological bioavailability of insulin was significantly larger (6.24%, p<0.05) for insulin-loaded hydrogels compared to free insulin. These results encourage further development of salecan-based hydrogels as vehicles for controlled insulin delivery following oral administration.

    更新日期:2018-09-22
  • Vitamin E encapsulation in plant-based nanoemulsions fabricated using dual-channel microfluidization: Formation, stability and bioaccessibility
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-21
    Shanshan Lv, Jiyou Gu, Ruojie Zhang, Yanhua Zhang, Haiyan Tan, David Julian McClements

    In this study, vitamin E was encapsulated in oil-in-water nanoemulsions fabricated using a dual-channel microfluidizer. A long chain triacylglycerol (corn oil) was used as a carrier oil and a biosurfactant (quillaja saponin) was used as a natural emulsifier. The impact of vitamin-to-carrier oil ratio on the formation, storage stability, and bioaccessibility of the nanoemulsions was determined. The lipid droplet size formed during homogenization increased with increasing vitamin content, which was attributed to a large increase in lipid phase viscosity. The storage stability of the nanoemulsions decreased as the vitamin content increased because the larger lipid droplets creamed faster. The rate and extent of lipid hydrolysis in the small intestine decreased as the vitamin content increased, probably because the vitamin molecules inhibited the ability of lipase to reach the triacylglycerols inside the lipid droplets. Vitamin bioaccessibility decreased as the vitamin level in the lipid phase increased, which was attributed to the reduced level of mixed micelles available to solubilize the tocopherols. The optimized nanoemulsion-based delivery system led to a relatively high vitamin bioaccessibility (53.9%). This research provides valuable information for optimizing delivery systems to increase the bioaccessibility of oil-soluble vitamins.

    更新日期:2018-09-22
  • Distribution of four bioactive flavonoids in maize tissues of five varieties and correlation with expression of the biosynthetic genes
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-21
    Zhongbao Zhang, Zhibin Liang, Longfei Yin, Qing X. Li, Zhongyi Wu

    Flavonoids are characteristic in maize and have diverse biological functions. C-Glycosylflavones are neuroprotective against β-amyloid-induced tau hyperphosphorylation and neurotoxicity in SH-SY5Y cells, which is relevant to Alzheimer’s disease prevention and treatment. The content of the C-glycosylflavones eriodictyol, luteolin, isoorientin and maysin varied in pollens, silks, tassels and seeds among five maize varieties. Eriodictyol content was high (51 - 322 ng/g dw) in pollens, while luteolin content was low (0.2 - 106 ng/g dw) in all four tissues. The isoorientin content was approximately 3- to 10-fold greater than eriodictyol in pollens and tassels, particularly in the hybrid M1 and sweet corn M5 varieties. Maysin content was high in most silks and tassels. The differential expression of five genes involved in the maysin biosynthesis correlated well with the profiles of the four flavonoids among tissues and varieties. The present study offers valuable data for maize breeding and the use of maize flavonoids as functional food components.

    更新日期:2018-09-22
  • New Degradation Pathways of the Key Aroma Compound 1-Penten-3-one during Storage of NFC Orange Juice
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-21
    Veronika Mall, Ines Sellami, Peter Schieberle

    1-Penten-3-one with its fresh and pungent smell at a very low odor threshold of 0.94 µg/L water has been characterized as impact aroma compound in many foods, such as grapefruit, orange juice, black tea, olive oil, or tomatoes. While its importance to the fresh sensation of unstored NFC orange juice was recently confirmed by aroma recombinates, a total loss was determined already after 4 weeks in NFC orange juice stored at 0 °C. Until now, the degradation pathway of this compound has not been clarified. Systematic model studies resulted in the identification of 1-hydroxy-3-pentanone and 4-hydroxy-3,8-decanedione as degradation products as well as S-(3-oxopentyl)-L-cysteine in the presence of the amino acid. In orange juice samples, it was found that the elevated content of 1-hydroxy-3-pentanone indicates a thermal processing, while S (3 oxopentyl)-L cysteine showed a significant increase during cold storage. Additionally, both compounds were identified in other food samples, such as commercial orange juices, pickled olives and olive oil, fresh tomatoes and commercial tomato juice, and black tea.

    更新日期:2018-09-22
  • Glyphosate Resistance Technology Has Minimal or No Effect on Maize Mineral Content and Yield
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-21
    Krishna N. Reddy, James V. Cizdziel, Martin M. Williams, Jude E. Maul, Agnes M. Rimando, Stephen O. Duke
    更新日期:2018-09-21
  • 更新日期:2018-09-21
  • The mechanism and implication of the sorption of perfluorooctanoic acid (PFOA) by varying soil size fractions
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-21
    Lei Xiang, Tao Xiao, Peng-Fei Yu, Hai-Ming Zhao, Ce-Hui Mo, Yan-Wen Li, Hui Li, Quan-Ying Cai, Don-Mei Zhou, Ming-Hung Wong

    Sorption of perfluorooctanoic acid (PFOA), a toxic and persistent organic pollutant, by various size fractions of an agricultural soil at environmentally relevant concentrations was evaluated. PFOA sorption to all fractions involved both film diffusion and intraparticle diffusion with the rate-limiting step by the latter. PFOA isotherm data fitted a linear model. Organic matter (OM), cation exchange capacity, pore volume, and the Brunauer-Emmett-Teller area played key roles in PFOA sorption. The sorption capacity followed the order of humic acid > clay (0.15-4.4mm) > fine silt (1.9-39.8mm) > coarse silt (17.3-79.4mm) > fine sand (45.7-316.2mm) > coarse sand (120-724.4mm), opposite to their contributions to overall PFOA sorption due to the influence of their percentage weight in the original soil. Percentage OM content was the dominant factor controlling the fraction contributions to overall PFOA sorption, demonstrating influence of the hydrophobic force on sorption. PFOA should be highly mobile and bioavailable in soil-crop systems due to the low logKoc values.

    更新日期:2018-09-21
  • Identification and Quantitation of Four New 2-Alkylthiazolidine-4-carboxylic Acids Formed in Or-ange Juice by a Reaction of Saturated Aldehydes with Cysteine
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-21
    Veronika Mall, Ines Sellami, Peter Schieberle

    Despite several technological efforts to maximize the quality and shelf life of chilled stored not-from-concentrate (NFC) orange juice, changes in the overall aroma profile might occur during storage. Besides the degradation of terpenoids, a loss of the aroma-active aldehydes, hexanal, octanal, nonanal and decanal as well-as of 1-penten-3-one were recently confirmed as a major cause for the changes in the aro-ma profile of orange juice even during storage under aseptic conditions at 0 °C. To unravel the fate of the aroma-active aldehydes, model experiments were carried out considering the oxidation into the corresponding acids as well as a reaction with free amino acids present in orange juice. The oxidation into the acids could be confirmed by isotope labeling experiments, additionally the reaction of the four aldehydes men-tioned above with L-cysteine yielded four new compounds identified as 2-alkylsubstituted thiazolidine-4-carboxylic acids. Their quantitation in orange juice samples by newly developed stable isotope dilution assays revealed that these acids were already present in the fresh samples, but were considerably increased after storage. Labeling experiments in orange juice administered with either labeled oc-tanal or labeled cysteine confirmed that the reaction quickly occurs in the juice. The data contribute another puzzle piece to the loss of aroma-active aldehydes during orange juice storage, which may also be relevant in other foods.

    更新日期:2018-09-21
  • 更新日期:2018-09-21
  • 更新日期:2018-09-21
  • Bio-based Large Tablet Controlled-Release Urea: Synthesis, Characterization, and Controlled-Released Mechanisms
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-20
    Lu Liu, Tianlin Shen, Yuechao Yang, Bin Gao, Yuncong C. Li, Jiazhuo Xie, Yafu Tang, Shugang Zhang, Zhonghua Wang, Jianqiu Chen

    To improve nitrogen (N) use efficiency and minimize environmental pollution caused by fertilizer over use, novel bio-based large tablet controlled-release urea (LTCRU) was prepared by using bio-based coating materials to coat large tablet urea (LTU) derived from urea prills (U). Nano fumed silica (NFS) was added to the bio-based coating materials to improve the slow-release properties. Surface area of the LTU and U was measured by three-dimension scanning. Compared with U, LTU had smaller surface area-to-weight ratio, which can reduce the coating materials. Scanning electron microscope analysis showed that the addition of NFS in bio-based coating materials reduced the porosity of the coating shells of LTCRUs and thus enhanced the N release longevity of the controlled-released fertilizer. Depending on the pores on the coating shells of LTCRU, two N release patterns were revealed. Because of the good release characteristics, the novel LTCRU shows great potential to support sustainable agricultural production.

    更新日期:2018-09-21
  • Storage of Micellar Casein Powders with and without Lactose: Consequences on Color, Solubility, and Chemical Modifications
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-20
    Sarah Nasser, Paulo De Sa Peixoto, Anne Moreau, Thomas Croguennec, Fabrice Bray, Christian Rolando, Frédéric J. Tessier, Alain Hédoux, Guillaume Delaplace
    更新日期:2018-09-20
  • Insecticide Exposure and Development of Nonalcoholic Fatty Liver Disease
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-20
    Jason S. Yang, Yeonhwa Park
    更新日期:2018-09-20
  • Ajothiolanes: 3,4-Dimethylthiolane Natural Products from Garlic (Allium sativum)
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-19
    Eric Block, Bérénice Dethier, Benjamin Bechand, Julien J. H. Cotelesage, Graham N. George, Kei Goto, Ingrid J. Pickering, Emerita Mendoza Rengifo, Robert Sheridan, Eileen Y. Sneeden, Linda Vogt
    更新日期:2018-09-20
  • Metabolism of the Strobilurin Fungicide Mandestrobin in Wheat
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-19
    Daisuke Ando, Takuo Fujisawa, Toshiyuki Katagi
    更新日期:2018-09-20
  • 更新日期:2018-09-20
  • Quantification of Ammonium Phosphatide Emulsifiers in Chocolate Using 31P NMR Spectroscopy
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-19
    Kirsten G. Malmos, Boris Gouilleux, Patrick Sønderskov, Tommy Andersen, Jens Viggo Frambøl, Thomas Vosegaard
    更新日期:2018-09-20
  • Dicationic ionic liquids of herbicide 2,4-D with reduced negative effects on environment
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-19
    Junfan Niu, Zhaopeng Zhang, Jingyue Tang, Gang Tang, Jiale Yang, Weichen Wang, Hong Huo, Na Jiang, Jianqiang Li, Yongsong Cao

    Due to high volatility and water solubility, 2,4-D can easily enter into the atmosphere and water bodies by volatilization, drift, leaching or runoff, which results in potential threats to the environment and human health. The physicochemical properties of pesticides can be regulated by preparing their ionic liquids. In this work, a series of dicationic ionic liquids (DILs) of 2,4-D were prepared to reduce its environmental risk and enhance herbicidal activity. The solubility, octanol–water partition coefficient, surface tension, and volatilization rate results of DILs showed that these properties could be optimized by choosing appropriate counter cations. Compared to 2,4-D ammonium salt, DILs have lower volatility, water solubility, and surface tension as well as higher lipophilicity. Benefiting from optimized physicochemical properties, DILs HIL8–12 exhibited better herbicidal activity against three typical broadleaf weeds than 2,4-D ammonium salt, and their fresh weight inhibition rates increased by 2.74–46.84%. The safety assessment experiment indicated that DILs were safer to wheat than commercialized forms of 2,4-D. The DILs could reduce environmental risk of 2,4-D caused by high volatility and water solubility and would be potential alternatives to its commercialized formulations.

    更新日期:2018-09-20
  • Plant Sr isotope ratios as affected by the Sr isotope ratio of the soil and of the external Sr inputs
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-19
    Agnese Aguzzoni, Michele Bassi, Peter Robatscher, Massimo Tagliavini, Werner Tirler, Francesca Scandellari

    The 87Sr/86Sr ratio of a produce is generally linked with the soil geological features of the growing areas. This study aimed at assessing to which extent the addition of external Sr by agricultural practices, like irrigation and mineral nutrient supply, influences this relationship. In a first experiment, oat plants in two soils reflected the 87Sr/86Sr of the soil. However, this link was significantly altered at increasing levels of external Sr soil supplies. In a second experiment, apple trees transplanted in pots modified their original 87Sr/86Sr, which became progressively closer to the soil Sr ratio. The addition of tap water and fertilizers, with different Sr isotopic signatures, slightly affected plant 87Sr/86Sr. Results confirm the potential of 87Sr/86Sr ratio as geographical tracer of agricultural commodities, but whenever the range of 87Sr/86Sr variability among soils from different geographical areas is narrow, the influence of external Sr-sources may smooth over these diversities.

    更新日期:2018-09-20
  • Glucose Glycation of α-Lactalbumin and β-Lactoglobulin in Glycerol Solutions
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-19
    Xiaoxia Chen, Lina Zhang, Bhesh Bhandari, Peng Zhou

    The glucose glycation of α-Lactalbumin and β-Lactoglobulin at 50 C in glycerol-based liquid system was investigated to evaluate the effect of water activity on glycation and site-specificity in glycerol matrix. Glycation extent during the reaction was determined using o-phthalaldehyde (OPA) method as well as ultra-performance liquid chromatography combined with electro-spray ionization mass spectrum (UPLC-ESI-MS). Glycation sites were identified by data-independent acquisition LC−MS (LC-MSE). The surface potential achieved by PyMOL and tertiary structure determined by circular dichroism (CD) were used to assist the analysis of the glycation site-specificity in glycerol matrix. The water activity of glycerol solutions was negatively correlated to the glycerol concentration. Results showed that the initial glycation rate in glycerol matrix was fitted to a liear equation in the first 48 h. Glycation accelerated with the increase of glycerol concentration, namely the decrease of water activity, regardless the native structure of protein. The glycation sites were identical at a similar DSP although achieved at different water activity, with 4 and 7 sites detected in α-lactalbumin and β-lactoglobulin, respectively. However, compared with the glycation sites in water based matrix, the site-specificity of glycation was affected by the glycerol matrix, depending on the native structure of proteins. Glycation was prone to occur at the reactive sites distributed on the surface of the proteins, particularly the region with positive potential.

    更新日期:2018-09-20
  • Neolignan Constituents with Potential Beneficial Effects in Prevention of Type 2 Diabetes from Viburnum fordiae Hance Fruits
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-19
    Chunchao Zhao, Jia Chen, Jianhua Shao, Jie Shen, Kehuan Li, Wenyan Gu, Sihui Li, Judi Fan

    Nine new neolignan glycosides (1−9), viburfordosides A−I, two new neolignans, fordianes A and B (10, 11), and seven known analogues (12−18) have been isolated and identified from the fruits of Viburnum fordiae Hance. The structures and absolute configurations of undescribed neolignan constituents were identified by chemical methods and spectroscopic analyses. The α-glucosidase inhibitory, ABTS•+ and DPPH• scavenging and anti-inflammatory activities of these secondary metabolites were evaluated. Some of them exhibited significant potency in inhibiting α-glucosidase and scavenging free radicals. Among fourteen metabolites that were found to have the capacity to inhibit NO production in LPS-stimulated RAW264.7 macrophage cells, compounds 2, 4, 6, 10, 11, 14, 17 and 18 were potent with IC50 values of 10.88 to 41.10 μM. These results support that V. fordiae fruits possessing the neolignan compounds may serve as both functional food and medicinal resource to prevent and treat type 2 diabetes (T2D).

    更新日期:2018-09-20
  • Acrylamide Defects the Expression Pattern of the Circadian Clock and Mitochondrial Dynamics in C57BL/6J Mice Liver and HepG2 Cells
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-19
    Xintong Tan, Tong Zhao, Zihan Wang, Jia Wang, Yijie Wang, Zhigang Liu, Xuebo Liu
    更新日期:2018-09-19
  • Concentrations and Distribution Patterns of Perfluoroalkyl Acids in Sewage Sludge and in Biowaste in Hesse, Germany
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-19
    Thorsten Stahl, Matthias Gassmann, Sandy Falk, Hubertus Brunn
    更新日期:2018-09-19
  • Acaricidal Activity of Cyclodipeptides from Bacillus amyloliquefaciens W1 against Tetranychus urticae
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-19
    Xing-Yu Li, Yue-Hu Wang, Jun Yang, Wen-Yan Cui, Peng-Jie He, Shahzad Munir, Peng-Fei He, Yi-Xin Wu, Yue-Qiu He
    更新日期:2018-09-19
  • 更新日期:2018-09-19
  • 更新日期:2018-09-19
  • Biotransformation of Artemisinin to 14-hydroxydeoxyartemisinin: C-14 hydroxylation by Aspergillus flavus
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-19
    Mangala Gowri Ponnapalli, MADHU BABU SURA, Renu Sudhakar, Gokulapriya Govindarajalu, Puran Singh Sijwali

    The biotransformation of the front-line antimalarial drug, artemisinin (1) by the filamentous fungus Aspergillus flavus MTCC-9167 was investigated. Incubation of compound 1 with A. flavus afforded a new hydroxy derivative (2) along with three known metabolites (3-5). The new compound was characterized as 14-hydroxydeoxyartemisinin (2) by extensive spectroscopic data analysis (IR, 1H, 13C NMR, HSQC, HMBC, COSY, NOESY and HR-ESIMS). The known metabolites were identified as deoxyartemisinin (3), artemisinin G (4), and 4α-hydroxydeoxyartemisinin (5). This is the first report of hydroxylation of a secondary methyl of artemisinin at C-14 by the fungus A. flavus, which is synthetically not accessible. In addition, these compounds were evaluated for their in vitro anti-plasmodial activity. Artemisinin G (4) exhibited IC50 values in submicromolar concentration compared to non-peroxidic metabolites.

    更新日期:2018-09-19
  • Targeting tumor metabolism with plant-derived natural products: emerging trends in cancer therapy
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-19
    Ângela Guerra, Maria Fatima Duarte, Iola F Duarte

    Recognition of neoplasic metabolic reprogramming as one of cancer’s hallmarks has paved the way for developing novel metabolism-targeted therapeutic approaches. The use of plant-derived natural bioactive compounds for this endeavor is especially promising, due to their diversiform structures and multiple targets. Hence, over the last decade, a growing number of studies have assessed the impact of phytochemicals on tumor cell metabolism, aiming at improving current knowledge on their mechanisms of action and, at the same time, at evaluating their potential as anticancer metabolic modulators. In this review, we focus on three classes of plant-derived compounds with promising anticancer activity - phenolic compounds, isoprenoids and alkaloids - to describe their effects on major energetic and biosynthetic pathways of human tumor cells. Such comprehensive and integrated account on the ability of these compounds to hit different metabolic targets is expected to contribute to the rational design and critical assessment of novel anticancer therapies based on natural product-mediated metabolic reprogramming.

    更新日期:2018-09-19
  • Changes of S-allylmercaptocysteine and γ-glutamyl-S-allylmercaptocysteine contents and their putative production mechanisms in garlic extract during the aging process
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-18
    Takuto Fujii, Toshiaki Matsutomo, Yukihiro Kodera

    γ-Glutamyl-S-allylmercaptocysteine (GSAMC), a putative precursor compound of S-Allylmercaptocysteine (SAMC), was isolated and identified from aged garlic extract (AGE). We analyzed the change of their contents in AGE during the aging process, chronologically 1 month to 22 months. The formation of these compounds occurred mostly during the early stage of the aging period: SAMC content reached a maximum at approximately 4 months, whereas GSAMC content reached a maximum at 1 month and then decreased during the subsequent aging period. In order to assess the possible relationship between the change of the two compounds during the aging process, we set up the model reactions with the hypothesis that GSAMC is produced from γ-glutamyl-S-allylcysteine (GSAC)/γ-gultamyl-S-1-propenylcysteine (GS1PC), and that SAMC is produced from GSAMC by endogenous γ-glutamyl transpeptidase (GGT) in garlic during the early stage of the aging process. We found that in the model reactions, SAMC was produced from GSAMC by the garlic protein fraction having GGT activity, and its production was suppressed by a GGT inhibitor. Furthermore, the production of GSAMC from allicin and GSAC/GS1PC was found in another model reaction. The reaction between allicin and GS1PC was faster than that between allicin and GSAC, and thus may be involved in the production of GSAMC in the early stage of aging process.

    更新日期:2018-09-19
  • Influence of Ternary Complexation Between Bovine Serum Albumin, Sodium Phytate and Divalent Salts on Turbidity and In Vitro Digestibility of Protein
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-18
    Elaine Kaspchak, Luciana Igarashi-Mafra, Marcos R. Mafra

    Phytate decreases mineral and protein availability and influences protein properties such as solubility and stability. The binding constants and turbidity data can help with the understanding of the influence of phytate and divalent salts on protein behavior. Ternary complexes formed between bovine serum albumin, sodium phytate and divalent salts were investigated by isothermal titration calorimetry, turbidity and in vitro protein digestibility. Results showed a positive entropy change and a negative and small enthalpy change due to electrostatic binding forces and ternary and binary complexes precipitation. The interaction was favored for the systems containing calcium and manganese, whereas those containing magnesium showed a low heat of interaction. Despite the high protein digestibility, the stability of divalent phytates in a wide pH range may decrease mineral bioavailability. These results can provide important insights for the study of mineral bioavailability and diverse processes that involve protein and minerals in several areas of knowledge.

    更新日期:2018-09-19
  • Nitrogen feeding strategies and metabolomic analysis to alleviate high-nitrogen inhibition on docosahexaenoic acid production in Crypthecodinium cohnii
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-18
    Liangsen Liu, Fangzhong Wang, Ji Yang, Xingrui Li, Jinyu Cui, Jing Liu, Mengliang Shi, Kang Wang, Lei Chen, Weiwen Zhang

    It is well known that high-nitrogen content inhibits cell growth and docosahexaenoic acid (DHA) biosynthesis in heterotrophic microalgae Crypthecodinium cohnii. In this study, two nitrogen feeding strategies, pulse-feeding and continuous-feeding, were evaluated to alleviate high-nitrogen inhibition effects on C. cohnii. The results showed that continuous-feeding with a medium solution containing 50% (w/v) yeast extract at 2.1 mL/h during 12-96 h was the optimal nitrogen feeding strategy for the fermentation process, when glucose concentration was maintained at 15-27 g/L during the same period. With the optimized strategy, 71.2 g/L of dry cell weight and DHA productivity of 57.1 mg/L/h were achieved. In addition, metabolomic analysis was applied to determine the metabolic changes during different nitrogen feeding conditions, and the changes in amino acids, polysaccharides, purines and pentose phosphate pathway were observed, providing valuable metabolite-level information for exploring mechanism of high-nitrogen inhibition effect, and further improving DHA productivity in C. cohnii.

    更新日期:2018-09-19
  • Could fecal phenylacetic and phenylpropionic acids be used as indicators of health status?
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-18
    Isabel Gutiérrez-Díaz, Tania Fernández-Navarro, Nuria Salazar, Begoña Bartolome, M. Victoria Moreno-Arribas, Patricia López, Ana Suarez, Clara G. de los Reyes-Gavilan, Miguel Gueimonde, Sonia Gonzalez

    Although most of the health effects attributed to polyphenols may be linked to their phenolic-derived metabolites, the role of the intestinal derived-phenolics in human health is still far from being well understood. We determined the profile of fecal phenolic-derived metabolites, microbiota, biomarkers of oxidative stress and inflammation, and daily intake of bioactive compounds in 71 elderly volunteers. Phenylacetic and phenylpropionic acids were the main phenolic metabolites present in feces. From them, phenylacetic acid was related with a more pro-oxidant and immune stimulated status, and both were negatively associated with fecal propionate, whereas phenylpropionic acid was directly related with the fecal concentration of acetate. Moreover, phenylacetic acid was negatively associated with the Bacteroides group and Clostridium cluster XIVa and positively with Lactobacillus. These results provide a rationale to explore the potential of fecal microbial phenolic-derived metabolites as possible biomarkers of health status in future studies focused on the elderly population.

    更新日期:2018-09-19
  • Biomarkers of Whole Grain Intake
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-18
    Shengmin Sang

    There is growing evidence that whole grain (WG) intake may prevent many chronic diseases. However, there are mixed results on this topic in human studies, due to a lack of accurate tools to assess the intake of WGs and individual metabolic variation. To better understand the effects of WGs on health maintenance and on the risk of chronic disease, there is an urgent need to identify the biomarkers for WG intake. The molecular signatures of WG intake remain undefined. This perspective gives an overview of the current knowledge, challenges, and future directions on the biomarkers of WG intake.

    更新日期:2018-09-19
  • Characterization of Daily Dietary Intake and the Health Risk of Neonicotinoid Insecticides for the U.S. Population
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-18
    Chi-Hsuan Chang, David MacIntosh, Bernardo Lemos, Quan Zhang, Chensheng Lu
    更新日期:2018-09-18
  • Improvement of the Color Stability of Cyanidin-3-glucoside by Fatty Acid Enzymatic Acylation
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-18
    Marta Guimarães, Nuno Mateus, Victor de Freitas, Luís Cruz
    更新日期:2018-09-18
  • The First Food-Derived Peptide Inhibitor of the Protein-Protein Interaction between Gain-of-Function PCSK9D374Yand the LDL Receptor
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-18
    Giovanni Grazioso, Carlotta Bollati, Jacopo Sgrignani, Anna Arnoldi, Carmen Lammi

    Proprotein Convertase Subtilisin/Kexin type 9 (PCSK9) is involved in cholesterol homeostasis, because it induces the low density lipoprotein receptor (LDLR) degradation. This protein may carry some positive or negative mutations: PCSK9D374Y is one of the most dangerous gain-of-function mutations. This paper reports the identification of the first food-derived peptide able to inhibit the protein-protein interaction (PPI) between PCSK9D374Y and LDLR. In fact, T9 (GQEQSHQDEGVIVR), an absorbable peptide deriving from lupin ß-conglutin, is able to impair the PPI between PCSK9D374Y and the LDLR, with an IC50 value equal to 285.6±2.46 μM. The consequence of this inhibition is an increase of the protein level of the LDLR located on hepatic cell membranes up to 74.3±4.4% and the restoration of the functional capability of HepG2 cells to uptake extracellular LDL up to 83.1±1.6%. Finally, the putative binding mode of T9 to the LDLR binding site located on PCSK9D374Y was postulated by in silico tools.

    更新日期:2018-09-18
  • Cyanidin-3-O-β-glucoside, a natural polyphenol, exerts proapoptotic effects on activated platelets and enhances megakaryocytic proplatelet formation
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-18
    Fuli Ya, Jinju Tian, Qing Li, Liyi Chen, Jing Ren, Yimin Zhao, Jian-Bo Wan, Wenhua Ling, Yan Yang

    This study investigated whether the anthocyanin cyanidin-3-O-β-glucoside (Cy-3-g) could affect platelet apoptosis and proplatelet formation in vitro. Thrombin-stimulated or resting human platelets and Meg-01 megakaryocytes were incubated with Cy-3-g (0, 0.5, 5 or 50 μM). We found that the percentage of the platelet mitochondrial membrane potential treated with 5 and 50 μM Cy-3-g was significantly higher than control (15.50% ± 3.24% and 29.77% ± 4.06% vs. 2.76% ± 1.33%, respectively; P<0.05). Treatment with 5 and 50 μM Cy-3-g significantly increased phosphatidylserine exposure compared with control (40.56% ± 10.53% and 76.62% ± 8.28% vs. 15.43% ± 3.93%, respectively; P<0.05). Moreover, Cy-3-g significantly increased the expression of Bax, Bak and cytochrome c while markedly decreasing Bcl-xL and Bcl-2 expression as well as stimulating caspase-3, caspase-9, caspase-8, Bid, and gelsolin cleavage in thrombin-activated platelets in a dose-dependent manner (P<0.05). However, no significant differences were observed in the apoptosis of resting platelets when treated with Cy-3-g (P>0.05). Furthermore, Cy-3-g significantly (P<0.05) enhanced cell viability (50 μM vs. control: 1.34 ± 0.01 vs. 0.35 ± 0.02), the number of colony-forming unit-megakaryocytes (50 μM vs. control: 38 ± 3 vs. 8 ± 3), CD41 expression (50 μM vs. control: 96.80% ± 2.55% vs. 25.57% ± 2.86%), DNA ploidy (16N) (50 μM vs. control: 19.73% ± 2.34% vs. 4.42% ± 1.96%) and proplatelet formation (50 μM vs. control: 27.5% ± 3.77% vs. 7.67% ± 2.25%) in Meg-01 cells. In conclusion, Cy-3-g promotes activated platelet apoptosis and enhances megakaryocyte proliferation, differentiation and proplatelet formation in vitro.

    更新日期:2018-09-18
  • Walnut (Juglans regia) peptides reverse sleep deprivation-induced memory impairment in rat via alleviating oxidative stress
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-18
    Shuguang Wang, Guowan Su, Qi Zhang, Tiantian Zhao, Yang Liu, Lin Zheng, Mouming Zhao

    The aim of this study was to determine the neuroprotective effects of walnut protein hydrolysates (WPH) against memory deficits induced by sleep deprivation (SD) in rat and further to identify and characterize the potent neuroprotective peptides against glutamate-induced apoptosis in PC12 cells. Results showed that a remarkable amelioration effect on behavioral performance in Morris water maze test was observed for WPH and its low molecular weight fraction WPHL, especially for WPHL. Additionally, a reduction of antioxidant defence (CAT, GSH-px, and SOD) and an increase of MDA content induced by SD were normalized in brain of rat after oral administration of WPH and WPHL. Then three neuroprotective peptides including GGW, VYY and LLPF were identified from WPHL, which could protect PC12 cells against glutamate-induced apoptosis with relative cell viability of 78.29 ± 3.09%, 80.65 ± 1.74% and 83.97 ± 3.06% respectively, versus glutamate group 48.61 ± 3.99%. The possible mechanism underlying their protective effects of GGW and VYY could be related to their strong radical scavenging activity as well as their ability to reduce ROS production and the depletion of SOD and GSH-px in PC12 cells. Notably, the marked neuroprotective effects of LLPF, which did not show obvious free radical scavenging activity in vitro, could be attributed to its strong effects on inhibiting Ca2+ influx and MMP collapse. Additionally, all these peptides could regulate the expression of apoptosis-related proteins (Bax and Bcl-2). Therefore, walnut peptides might be regarded as the potential nutraceuticals against neurodegenerative disorders associated with memory deficits.

    更新日期:2018-09-18
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  • 更新日期:2018-09-18
  • CRISPR/Cas9-mediated Stearoyl-CoA Desaturase 1 (SCD1) Deficiency Affects Fatty Acid Metabolism in Goat Mammary Epithelial Cells
    J. Agric. Food Chem. (IF 3.412) Pub Date : 2018-09-17
    Huibin Tian, Jun Luo, Zhifei Zhang, Jiao Wu, Tianying Zhang, Sebastiano Busato, Lian Huang, Ning Song, Massimo Bionaz
    更新日期:2018-09-18
Some contents have been Reproduced with permission of the American Chemical Society.
Some contents have been Reproduced by permission of The Royal Society of Chemistry.
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