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  • Saponins from sea cucumber and their biological activities
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-06-22
    Yingcai Zhao, Changhu Xue, Tiantian Zhang, YuMing Wang

    Sea cucumbers, belonging to the phylum Echinodermata, have been valued for centuries as a nutritious and functional food with various bioactivities. Sea cucumbers can produce highly active substances, notably saponins, the main secondary metabolites, which are the basis of their chemical defense. The saponins are mostly triterpene glycosides with triterpenes or steroid in aglycone, which possess multiple biological properties including anti-tumor, hypolipidemic activity, improvement of nonalcoholic fatty liver, inhibition of fat accumulation, anti-hyperuricemia, promotion of bone marrow hematopoiesis, anti-hypertension, etc. Sea cucumber saponins have received attention due to their rich sources, low toxicity, high efficiency, and few side effects. This review summarizes current research on the structure and activities of sea cucumber saponins based on the physiological and pharmacological activities from source, experimental models, efficacy and mechanisms, which may provide a valuable reference for the development of sea cucumber saponins.

    更新日期:2018-06-25
  • Characterization of Nicotine Catabolism through a Novel Pyrrolidine Pathway in Pseudomonas sp. S-1
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-06-22
    Dandan Pan, Mengmeng Sun, Yawen Wang, Pei Lv, Xiangwei Wu, Qing X. Li, Haiqun Cao, Rimao Hua

    Nicotine is a major toxic alkaloid in wastes generated from tobacco production and cigarette manufacturing. In the present work, a nicotine-degrading bacterial strain was isolated from tobacco powdery waste. The isolate was identified as Pseudomonas sp. S-1 based on morphology, physiology, and 16S rRNA gene sequence. Suitable conditions of isolate S-1 for nicotine degradation were pH 7.0 and 30 C. Catabolic intermediates of nicotine were isolated with preparative-HPLC and characterized with LC-HRMS and NMR. The catabolic pathways of nicotine were involved in dehydrogenation, oxidation, hydrolysis, and hydroxylation. Interestingly, nicotine catabolism in strain S-1 undergoes a new pyrrolidine pathway that differs from the other three catabolic pathways in bacterial species. This work sheds light on catabolic diversity of nicotine and heteroaromatics.

    更新日期:2018-06-25
  • Structural characterization of willow bark lignin
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-06-22
    Jinze Dou, Hoon Kim, Yanding Li, Dharshana Padmakshan, Fengxia Yue, John Ralph, Tapani Vuorinen

    Understanding the chemical structure of lignin in willow bark (WB) is an indispensable step to design how to separate its fiber bundles. The whole cell wall and enzyme lignin (EL) preparations sequentially isolated from ball-milled WB, willow inner bark (WIB), and willow wood (WW) were comparatively investigated by NMR spectroscopy and three classical degradative methods, i.e., alkaline nitrobenzene oxidation, derivatization followed by reductive cleavage, and analytical thioacidolysis. All results demonstrated the guaiacyl (G) units were predominant in the WB lignin over syringyl (S) and minor p-hydroxyphenyl (H) units. Moreover, the monomer yields and S/G ratio raised progressively from WB to WIB and to WW, indicating the lignin may be more condensed in WB than in other tissues. Additionally, major interunit linkage substructures (β-aryl ethers, phenylcoumarans and resinols) together with cinnamyl alcohol end-groups were relatively quantified by 2D NMR spectroscopy. WB and WIB were rich in pectins and proteins which were present in large quantities also in the EL preparations.

    更新日期:2018-06-25
  • 更新日期:2018-06-22
  • Fabrication and Characterization of β-Lactoglobulin-Based Nanocomplexes Composed of Chitosan Oligosaccharides as Vehicles for Delivery of Astaxanthin
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-06-22
    Chengzhen Liu, Zhuzhu Liu, Xun Sun, Shuaizhong Zhang, Shuhui Wang, Fuxian Feng, Dongfeng Wang, Ying Xu
    更新日期:2018-06-22
  • Effect of Sodium Chloride and Sodium Bicarbonate on the Physicochemical Properties of Soft Wheat Flour Doughs and Gluten Polymerization
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-06-22
    Gengjun Chen, Laura Ehmke, Rebecca Miller, Pierre Faa, Gordon Smith, Yonghui Li
    更新日期:2018-06-22
  • 更新日期:2018-06-22
  • A New Compound Isolated from the Reduced Ribose–Tryptophan Maillard Reaction Products Exhibits Distinct Anti-inflammatory Activity
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-06-22
    Dan Qin, Lin Li, Jing Li, Jinlong Li, Di Zhao, Yuting Li, Bing Li, Xia Zhang
    更新日期:2018-06-22
  • 更新日期:2018-06-22
  • 更新日期:2018-06-22
  • Parapyruvate, an Impurity in Pyruvate Supplements, Induces Senescence in Human Fibroblastic Hs68 Cells via Inhibition of the α-Ketoglutarate Dehydrogenase Complex
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-06-22
    Shih-Chung Chang, Inn Lee, Hua Ting, Yuan-Jhe Chang, Nae-Cherng Yang

    Commercial dietary supplements of calcium pyruvate claim to be beneficial for losing weight, increasing muscle endurance, and regulating metabolism. Most industrial preparations have some impurities, including parapyruvate. Parapyruvate is an inhibitor of the α-ketoglutarate dehydrogenase complex (KGDHC). However, the effect and mechanism of parapyruvate on cell senescence and the content of parapyruvate in the dietary supplements of calcium pyruvate are unknown. In this study, we prepared pure parapyruvate with a purity of 99.8 ± 0.1% and investigated its ability to inhibit KGDHC activity and affect fibroblast senescence. Parapyruvate dose-dependently decreased KGDHC activity, with an IC50 of 4.13 mM, and induced Hs68 cell senescence. Calcium ions, a KGDHC activator, antagonized the senescent effects of parapyruvate. The parapyruvate content was 1.4 ± 0.1% to 10.6 ± 0.2% in five brands of calcium pyruvate supplements. In this study, we showed that parapyruvate strongly induces Hs68 cell senescence by inhibiting KGDHC activity. Because of its KGDHC inhibition activity, the parapyruvate content should be an important issue for the food safety of calcium pyruvate supplements.

    更新日期:2018-06-22
  • Ginsenoside 25-OCH3-PPD promotes activity of LXRs to ameliorate P2X7R-mediated NLRP3 inflammasome in the development of hepatic fibrosis
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-06-22
    Xin Han, Jian Song, Li-hua Lian, You-li Yao, Dan-yang Shao, Ying Fan, Li-shuang Hou, Ge Wang, Shuang Zheng, Yan-ling Wu, ji-xing nan

    Ginseng is widely used in energy drinks, dietary supplements and herbal medicines, and its pharmacological actions are related with energy metabolism. As an important modulating energy metabolism pathway, liver X receptors (LXRs) can promote the resolving of hepatic fibrosis and inflammation. The present study aims to evaluate the regulation of 25-OCH3-PPD, a ginsenoside isolated from Panax ginseng, against hepatic fibrosis and inflammation in thioacetamide (TAA)-stimulated mice by activating LXRs pathway. 25-OCH3-PPD decreases serum ALT/AST levels and improves the histological pathology of liver in TAA-induced mice; attenuates transcripts of pro-fibrogenic markers associated with hepatic stellate cell activation; attenuates the levels of pro-Inflammatory cytokines and blocks apoptosis happened in liver; inhibits NLRP3 inflammasome by affecting P2X7R activation; regulates PI3K/Akt and LKB1/AMPK-SIRT1. 25-OCH3-PPD also facilitates LX25Rs and FXR activities decreased by TAA stimulation. 25-OCH3-PPD also decreases α-SMA via regulation of LXRs and P2X7R-NLRP3 in vitro. Our data suggest the possibility that 25-OCH3-PPD promotes activity of LXRs to ameliorate P2X7R-mediated NLRP3 inflammasome in the development of hepatic fibrosis.

    更新日期:2018-06-22
  • Characterization of endogenous nanoparticles from roasted chicken breasts
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-06-22
    Xunyu Song, Lin Cao, Shuang Cong, Yukun Song, Mingqian Tan

    Emergence of endogenous nanoparticles in thermally processed food has aroused much attention due to their unique properties and potential biological impact. The aim of this study was to investigate the presence of fluorescence nanoparticles in roasted chicken breasts, elemental composition, physico-chemical properties and their molecular interaction with human serum albumin (HSA). Transmission electron microscopy analysis revealed that the foodborne nanoparticles from roasted chicken were nearly spherical with an average particle size of 1.7 ± 0.4 nm. The elemental analysis of X-ray photoelectron spectroscopy showed the composition of nanoparticles as 47.4% C, 25.8% O and 26.1% N. The fluorescence of HSA was quenched by the nanoparticles following a static mode, and the molecular interaction of nanoparticles with HSA was spontaneous (ΔG°<0), where hydrogen bonding and van der Waals forces played an important role during HSA-nanoparticles complex stabilization through thermodynamic analysis by isothermal titration calorimetry. The principal location of the nanoparticles binding site on HSA was primarily in site I as determined by site-specific marker competition. The conformational of HSA was also changed and ɑ-helical structure decreased in the presence of nanoparticles. Our studies revealed that fluorescent nanoparticles were produced after roasting of chicken breast at 230 °C for 30 min for the first time. The obtained nanoparticles can interact with HSA in a spontaneous manner, thus providing valuable insight into foodborne NPs as well as their effects to human albumin protein.

    更新日期:2018-06-22
  • Bulk, Foam, and Interfacial Properties of Tannic Acid/Sodium Caseinate Nanocomplexes
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-06-21
    Fuchao Zhan, Jing Li, Yuntao Wang, Minqi Shi, Bin Li, Feng Sheng
    更新日期:2018-06-22
  • In Vitro Determination of Protein Conjugates in Human Cells by LC-ESI-MS/MS after Benzyl Isothiocyanate Exposure
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-06-21
    Carla Kühn, Tobias von Oesen, Corinna Herz, Monika Schreiner, Franziska S. Hanschen, Evelyn Lamy, Sascha Rohn
    更新日期:2018-06-22
  • Effects of Fiber Purified Extract of Carob Fruit on Fat Digestion and Postprandial Lipemia in Healthy Rats
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-06-21
    A. Macho-González, A. Garcimartín, F. Naes, M. E. López-Oliva, A. Amores-Arrojo, M. J. González-Muñoz, S. Bastida, J. Benedí, F. J. Sánchez-Muniz
    更新日期:2018-06-22
  • 更新日期:2018-06-22
  • Realistic Evaluation of Titanium Dioxide Nanoparticle Exposure in Chewing Gum
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-06-20
    Fabio Fiordaliso, Claudia Foray, Monica Salio, Mario Salmona, Luisa Diomede
    更新日期:2018-06-22
  • Unexpected Rheological Behavior of a Hydrophobic Associative Shellac-Based Oligomeric Food Thickener
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-06-20
    Jianan Gao, Kun Li, Juan Xu, Wenwen Zhang, Jinju Ma, Lanxiang Liu, Yanlin Sun, Hong Zhang, Kai Li
    更新日期:2018-06-22
  • Characterization and Quantification of Oligosaccharides in Human Milk and Infant Formula
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-06-20
    Rose M. Nijman, Yan Liu, Apichaya Bunyatratchata, Jennifer T. Smilowitz, Bernd Stahl, Daniela Barile
    更新日期:2018-06-22
  • Fabrication of a Flexible Gold Nanorod Polymer Metafilm via a Phase Transfer Method as a SERS Substrate for Detecting Food Contaminants
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-06-20
    Nan Yang, Ting-Ting You, Yu-Kun Gao, Chen-Meng Zhang, Peng-Gang Yin
    更新日期:2018-06-22
  • 更新日期:2018-06-22
  • 更新日期:2018-06-22
  • Hydrophobic nanoprecipitates of β-cyclodextrin/avermectins inclusion compounds reveal insecticide activity against Aedes aegypti larvae and low toxicity against fibroblasts
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-06-21
    Ana Maria dos Santos Moreira, Vanessa Bittencourt, Fábio Costa, Maria Elena de Lima, Miriam Lopes, Warley S. Borges, Gustavo Martins, Jeferson da Silva, Ângelo M. Leite Denadai, Keyller Bastos Borges

    In the present work, hydrophobic nanoprecipitates (HNPs) of inclusion complexes formed between -cyclodextrin (CD) and the avermectins (AVMs) named eprinomectin (EPRI) and ivermectin (IVER) were synthesized and characterized, and their larvicidal activity against Aedes aegypti and human safety against fibroblasts were evaluated. Initially, thermogravimetric analysis/differential thermal analysis data revealed that inclusion increased the thermal stability of AVMs in the presence of CD. Nuclear magnetic resonance experiments and density functional theory calculations pointed out the inclusion of the benzofuran ring of the two AVMs in the βCD cavity. Isothermal titration calorimetry experiments allowed identification of different binding constants for EPRI/CD (Kb = 1060) and CD/IVER (Kb = 1700) systems, despite the structural similarity. Dynamic light scattering titrations of AVMs’ dimethyl sulfoxide solution in CD aqueous solution demonstrated that the formed HNPs have lower sizes in the presence of CD. Finally, the inclusion of EPRI in βCD increased its larval toxicity and reduced its human cytotoxicity while for IVER/βCD no beneficial effect was observed upon inclusion. These results were rationalized in terms of structural differences between the two molecules. Finally, the EPRI/βCD complex has great potential as an insecticide against Aedes aegypti larvae with high human safety.

    更新日期:2018-06-22
  • Sensory quality, physicochemical attributes, polyphenol profiles and residual fungicides in strawberries from different disease control treatments
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-06-21
    Marvin Abountiolas, Katrina Kelly, Yavuz Yagiz, Zheng Li, Gail Mahnken, Wlodzimierz Borejsza-Wysocki, Maurice R. Marshall, Charles Sims, Natalia Peres, Maria Cecilia Do Nascimento Nunes

    Using alternative agricultural practices in combination with proper postharvest handling has become a major goal to improve fresh produce quality. Here, two different strawberry (Fragaria × ananassa) genotypes were used as a model to study the impact of repeated, reduced-fungicide or no-fungicide applications on the sensory quality, physicochemical attributes, polyphenol profiles and residual fungicide in strawberries. Strawberries grown under reduced-fungicide applications had similar or better physicochemical quality than conventionally and organically-grown fruit and lower levels of fungicide residues than conventional fruit. Overall, flavor- and health-related attributes of strawberries from reduced-fungicide applications were intermediate between conventional and organic fruit. Thus, growing strawberries with reduced-fungicide applications can be an alternative to conventional or organic agricultural practices.

    更新日期:2018-06-22
  • Two poplar hybrid clones differ in phenolic antioxidant levels and polyphenol oxidase activity in response to high salt and boron irrigation
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-06-20
    Khanh Nguyen, Carlos Cuellar, Prabhjot Sandy Mavi, Danika LeDuc, Gary Bañuelos, Monika Sommerhalter

    Poplar hybrids can be used for selenium (Se) and boron (B) phytoremediation under saline conditions. The phenolic antioxidant stress response of two salt and B tolerant poplar hybrids of parentage Populus trichocarpa x nigra x deltoides was studied using high performance liquid chromatography (HPLC) and absorption based assays to determine the antioxidant capacity, total phenolic content, hydroxycinnamic acid levels, and the enzyme activity of L-phenylalanine ammonia lyase (PAL), polyphenol oxidase (PPO), phenol peroxidase (POD), and laccase. Most remarkable was the contrasting response of the two poplar clones for PPO activity and phenolic levels to irrigation with high salt/B water. To cope with stressful growing conditions, only one clone increased its phenolic antioxidant level and each clone displayed different PPO isoform patterns. Our study shows that poplar hybrids of the same parentage can differ in their salt/B stress coping mechanism.

    更新日期:2018-06-22
  • Food Allergen Epitope Mapping
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-06-20
    Changqi Liu, Shridhar Krishna Sathe

    With the increased global awareness and rise in food allergies, a multifold interest in food allergens is evident. The presence of undeclared food allergens results in expensive food recalls and increase risks of anaphylaxis for the sensitive individuals. Regardless of the allergenic food, the immunogen needs to be identified and detected before making any efforts to inactivate/eliminate it. In type I food allergies, protein immunogen cross-links IgEs leading to basophil/mast cell degranulation resulting in the symptoms that range from mild irritation to anaphylaxis. A portion/part of the protein, known as the epitope, can interact either with antibodies to elicit allergic reactions or with T-cell receptors to initiate allergic sensitization. Antibody-recognized epitopes can be either linear sequence of amino acids (linear epitope) or three dimensional motif (conformational epitope) while T-cell receptor-recognized epitopes are exclusively linear peptides. Identifying and characterizing human allergy-relevant epitopes are important for allergy diagnosis/prognosis and immunotherapy, and for developing food processing methods that can reduce/eliminate immunogencity/immunoreactivity of the allergen.

    更新日期:2018-06-22
  • Traversal of the Blood Brain Barrier by Cleavable L-Lysine Conjugates of Apigenin
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-06-20
    Tsung-Yun Wong, Ming-Shian Tsai, Lih-Ching Hsu, Shu-Wha Lin, Pi-Hui Liang

    Apigenin, a flavone abundant in parsley and celery, is known to act on several CNS receptors, but its very poor water solubility (< 0.001 mg/mL) impedes its absorption in vivo and prevents clinical use. Herein, apigenin was directly conjugated with glycine, L-phenylalanine, and L-lysine to give the corresponding carbamate derivatives, all of which were much more soluble than apigenin itself (0.017, 0.018, and 0.13 mg/mL, respectively). The Lys-apigenin carbamate 10 had a temporary sedative effect on the mice within five minutes of intraperitoneal administration (single dose of 0.4 mg/g), and could be detected in the mice brain tissues at a concentration of 0.82 μg/g of intact Lys-apigenin carbamate 10 and 0.42 ug/g of apigenin at 1.5 h. This study accomplished the delivery of apigenin across the BBB in a manner that might be applicable to other congeners, which should inform the future development of BBB-crossing flavonoids.

    更新日期:2018-06-20
  • The Metabolism Distribution and Effect of Thiamethoxam After Oral Exposure in Mongolian racerunner (Eremias argus)
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-06-20
    Yinghuan Wang, Yang Zhang, Peng Xu, Baoyuan Guo, Wei Li

    Systematically evaluation of the metabolism, distribution and effect of thiamethoxam in mongolian racerunner (Eremias argus) were carried out after oral exposure. The HPLC equipped with Q Exactive focus was used for identification and concentration analysis of thiamethoxam and its metabolites. Percutaneous and urine excretions were the primary ways for the elimination of thiamethoxam and its metabolites, and the limiting factor was urine output. Demethylation thiamethoxam and clothianidin were the main metabolites of thiamethoxam in lizard. The CYP3A4, CYP3A7 and CYP2C9 played a crucial role in the metabolism process. Aldehyde oxidase only dominated the nitro-reduction process of demethylation thiamethoxam and clothianidin. Glutathione S-transferase might be related to the clearance process of thiamethoxam and its metabolites. The findings indicated that thiamethoxam might pose potential carcinogenic and hepatic injury risk to lizards. The results enrich and supplement the knowledge of the environmental fate of thiamethoxam in reptiles.

    更新日期:2018-06-20
  • In Vitro Pepsin Digestibility of Cooked Proso Millet (Pancium miliaceum L.) and Related Species from Around the World
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-06-20
    Paridhi Gulati, Shangang Jia, Aixia Li, David Richard Holding, Dipak Santra, Devin Jerrold Rose

    Thirty-three accessions of proso millet (Panicum miliaceum) with different countries of origin were screened for their pepsin digestibility after cooking to identify samples with high digestibility. The pepsin digestibility of all samples ranged from 26 to 57% (average 32%). There were no apparent differences in protein profiles (SDS-PAGE) of samples with the lowest, intermediate, and highest digestibilities. However, LC-MS/MS analysis revealed a negative correlation between pepsin digestibility and peptides that matched to high molecular weight proteins (24 kDa) from Panicum hallii with regions of contiguous hydrophobic amino acids. Low digestibility upon cooking was also observed for other species from the Panicum genus, such as little millet, switchgrass and panicgrass, which suggests a unique inherent property of the genus. The obtained results from this study may form a basis for in-depth analysis of proso proteins that may help in developing new cultivars with higher digestibility upon cooking.

    更新日期:2018-06-20
  • Untargeted 1H-NMR based metabolomics analysis of urine and serum profiles after consumption of lentils, chickpeas and beans: an extended meal study to discover dietary biomarkers of pulses
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-06-19
    Francisco Madrid-Gambin, Carl Brunius, Mar Garcia-Aloy, Sheila Estruel-Amades, Rikard Landberg, Cristina Andres-Lacueva

    High legume intake has been shown to have beneficial effects on the health of humans. The use of nutritional biomarkers, as a complement to self-reported questionnaires, could assist in evaluating dietary intake and downstream effects on human health. The aim of this study was to investigate potential biomarkers of the consumption of pulses (i.e. white beans, chickpeas and lentils) by using untargeted NMR-based metabolomics. Meals rich in pulses were consumed by a total of 11 participants in a randomized crossover study and multilevel partial least squares regression was employed for paired comparisons. Metabolomics analysis indicated that trigonelline, 3-methylhistidine, dimethylglycine, trimethylamine and lysine were potential, though not highly specific, biomarkers of pulse intake. Furthermore, monitoring of these metabolites for a period of 48 h after intake revealed a range of different excretion patterns among pulses. Following the consumption of pulses, a metabolomic profiling revealed that the concentration ratios of trigonelline, choline, lysine and histidine were similar to those found in urine. In conclusion, this study identified potential urinary biomarkers of exposure to dietary pulses and provided valuable information about the time-response effect of these putative biomarkers.

    更新日期:2018-06-20
  • Recent Developments and Digital Perspectives in Food Safety and Authenticity
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-06-19
    Jan Fritsche

    Food Safety is of fundamental importance for the food processing industry, food retailers and distributors, as well as for the competent authorities because of its potentially direct impact on consumers’ health. Next to the prevention of microbiological, chemical, and physical hazards increasing efforts are currently made to combat risks associated with food fraud or food authenticity. Food Safety Management Systems nowadays comprise food safety, food defense, and food fraud prevention measures trying to cope with the increasing complexity and globalization of the food supply chains. Future digital opportunities include the prediction of food safety and food authenticity issues by handling structured and unstructured data retrieved from various sources and origins in order to ensure consumers’ health and to minimize economical losses.

    更新日期:2018-06-20
  • Characterization of gene expression profile, phenolic composition and antioxidant capacity in red-fleshed grape berries and their wines
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-06-19
    Qianyu Yue, Lili Xu, Guangqing Xiang, Xin Yu, Yuxin Yao

    Gene expression profile, phenolic composition and antioxidant capacity were evaluated in red-fleshed berries and their wines (RF berries and wines) from new grape genotypes. Transcriptomic analysis revealed that ten metabolic pathways involved in polyphenol synthesis and catabolism were significantly altered, and 13 genes related to the biosynthesis and transport of phenolics were largely up-regulated in RF berries compared to that of Cabernet Sauvignon (CS). Expression of MybA1 was associated with anthocyanin accumulation in red flesh. Additionally, RF berries and wines contained higher concentrations of total anthocyanins, phenols, flavonoids and proanthocyanidins than that in CS berries and wine. Particularly, diglucosides of malvidin, peonidin, delphinidin and cyanidin were present in red flesh and RF wines, but they were undetectable or present at very low concentrations in CS flesh and wine. Cinnamic acid and ferulic acid were clearly increased in the RF wines compared to that in the CS wine. Additionally, the RF wines had higher antioxidant capacity than that in the CS wine, and total anthocyanin content was significantly correlated to antioxidant capacity. This research provides insight into the mechanisms underlying grape flesh coloration and the composition of phenolic compounds in RF berries and wines.

    更新日期:2018-06-20
  • Exploration of the Relationship between Intestinal Colostrum or Milk, and Serum Metabolites in Neonatal Calves by Metabolomics Analysis
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-06-19
    Yunxia Qi, Xiaowei Zhao, dongwei huang, xiaocheng pan, huiling zhao, Han Hu, Guanglong Cheng, yongxin yang

    In contrast to colostral immunoglobulins, changes in metabolite composition of ingested colostrum in the gut have received little attention. Here, we characterized the metabolite profiles of colostrum and milk, ingested colostrum and milk, and serum of neonatal calves by LC–MS and GC–MS metabolomics approaches. Colostrum and milk underwent similar changes in metabolite profiles in the gut after being ingested. These changes were characterized by increase in methionine, glutamate, thymine and phosphorylcholine. After ingestion, colostrum concentrations of several metabolites, such as γ-aminobutryic acid, glutamate, cinnamic acid, and thymine increased, whereas concentrations of D-ribose, and arginine decreased. These increases and decreases occurred in a time-dependent manner, and were associated with alanine, aspartate, glutamate, and pyrimidine metabolism, and valine, leucine and isoleucine biosynthesis, respectively. Meanwhile, similar changes in serum metabolites were also observed in neonatal calves fed colostrum, which implies that colostrum metabolites are transported across the small intestine and into the bloodstream. In addition, several metabolites of ingested milk were detected in the gut, and were also transferred to the bloodstream. These metabolites were related to phenylalanine, tyrosine, tryptophan, valine, leucine and isoleucine biosynthesis, the citrate cycle, and histidine metabolism. These findings reveal that the serum metabolome of neonatal calves’ changes as a result of ingesting colostrum, which can provide health-related benefits in early life.

    更新日期:2018-06-20
  • Implication on significance of the dietary compatibility: Based on the antioxidant and anti-inflammatory interactions with different ratios of hydrophilic and lipophilic antioxidants among four daily agricultural crops
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-06-19
    Yao Pan, Hongyan Li, Shi-Lian Zheng, Bing Zhang, zeyuan deng

    The hydrophilic extracts of eggplant peel (HEEP) and purple sweet potato (HEPP), lipophilic extracts of tomato (LET) and carrot (LEC) were mixed in different ratios to assess the significance on the compatibility of aliments, based on their antioxidant and anti-inflammatory interactions in H9c2 cells. The results indicated that groups of some combinational extracts (HEPP-HEEP: F1/10, LEC-HEEP: F3/10, LEC-HEPP: F3/10) showed stronger synergistic antioxidant and anti-inflammatory effects than individual groups. For example, the GPx activity of LEC-HEEP (F3/10) group (86.71 ± 1.88) was higher than that in HEEP (79.97 ± 1.68) and LEC (77.31 ± 1.85) groups. The level of ROS was 30.37 ± 0.25 in LEC-HEEP (F3/10) group while the levels were 34.34 ± 0.36 and 46.23 ± 0.51 in HEEP group and LEC group, respectively. And the level of MDAwas 1.82 ± 0.24 in the LEC-HEEP (F3/10) group while the levels were 2.48 ± 0.13 and 3.01 ± 0.24 in HEEP group and LEC group, respectively. The expressions of inflammatory mediators (IL-1β, IL-6, IL-8) and cell adhesion molecules (VCAM-1, ICAM-1) showed the similar tendency. However, some groups (LET-LEC: F5/10, LET-LEC: F9/10, LET-HEPP: F7/10) showed antagonistic effects based on these indicators. The principal component analysis showed that samples could be defined by two principal components. PC1: the main phenolic acids and flavonoids. PC2: carotenoids. Moreover, phenolics and anthoyanins were in the majority in synergistic groups, and carotenoids were in the majority in antagonistic groups. These results indicated that there exist synergistic or antagonistic interactions of aliments on anti-oxidation and anti-inflammation, which implied the significance of food compatibility.

    更新日期:2018-06-20
  • Trace level analysis of Sarin and VX in food using normal phase silica gel and ultra-performance liquid chromatography-time of flight mass spectrometry (UPLC-TOF-MS)
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-06-19
    Su Y Bae, Mark D Winemiller

    Ultra-performance liquid chromatography/electrospray ionization mass spectrometry was used for the trace level determination of isopropyl methylphosphonofluoridate (Sarin, GB) and (O-ethyl S-[2-(diisopropylamino)ethyl] methylphosphonothioate (VX) after extraction from various foods. A method utilizing normal phase silica gel was developed for the sample preparation and extraction of VX and GB from food. The extraction efficiencies of the normal phase silica gel method for VX was compared to those of other commercial solid phase extraction media and was found to be comparable. Sarin was found to be incompatible with both the mixed mode cation exchange (MCX) sorbents and QuEChERS methods that are commercially available but was successful with the normal phase silica gel method. The linear range of quantitation for VX was 0.1-330 ng/mL and for GB was 20-1200 ng/mL. The average recover-ies of VX and GB from the various food matrices along with the corresponding relative standard devia-tions (RSDs) are reported.

    更新日期:2018-06-20
  • Effects of Cyclic Fatty Acid Monomers from Heated Vegetable Oil on Markers of Inflammation and Oxidative Stress in Male Wistar Rats
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-06-19
    Jean Mboma, Nadine Leblanc, Paul Angers, Amandine Rocher, Claire Vigor, Camille Oger, Guillaume Reversat, Joseph Vercauteren, Jean-Marie Galano, Thierry Durand, Helene Jacques

    This study assesses the effects of cyclic fatty acid monomers (CFAM) from heated vegetable oils on oxidative stress and inflammation. Wistar rats were fed either of the four diets for 28 days: canola oil (CO), canola oil and 0.5% CFAM (CC), soybean oil (SO), and soybean oil and 0.5% CFAM (SC). Markers of oxidative stress and inflammation were respectively determined by micro-LC-MS/MS and ELISA kits. ANOVA for a 2 x 2 factorial design was performed to determine the CFAM and oil effects and interactions between these two factors at P≦0.05. For significant interactions, a post hoc multiple comparison test was performed, i.e. Tukey HSD test. CFAM induced higher plasma levels of 15-F2t-IsoP (CC: 396 ± 43 ng/mL, SC: 465 ± 75 ng/mL vs. CO: 261 ± 23 ng/mL and SO: 288 ± 35 ng/mL, P<0.05). Rats fed the SC diet had higher plasma 2,3-dinor-15-F2t-IsoP (SC: 145 ± 9 ng/mL vs. CC: 84 ± 8 ng/mL , CO: 12 ± 1 ng/mL, and SO: 12 ± 1 ng/mL, P<0.05), urinary 2,3-dinor-15-F2t-IsoP (SC: 117 ± 12 ng/mL vs. CC: 67 ± 13 ng/mL, CO: 15 ± 2 ng/mL , and SO: 18 ± 4 ng/mL, P<0.05), and plasma IL-6 (SC: 57 ± 10 pg/mL vs. CC: 48 ± 11 pg/mL, CO: 46 ± 9 pg/mL, and SO: 44 ± 4 pg/mL, P<0.05) than the other three diet groups. These results indicate that CFAM increased the levels of markers of oxidative stress, and those effects are exacerbated by a CFAM-high-linoleic acid diet.

    更新日期:2018-06-20
  • Metabolomics investigation reveals 8-C N-ethyl-2-pyrrolidinone substituted flavan-3-ols are potential marker compounds of stored white teas
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-06-19
    Weidong Dai, Junfeng Tan, Meiling Lu, Yin Zhu, Pengliang Li, Qunhua Peng, Li Guo, Yue Zhang, Dongchao Xie, Zhengyan Hu, Zhi Lin

    White teas of different stored ages have varied flavor, bioactivity, and commercial value. In this study, liquid chromatography-mass spectrometry (LC-MS)-based metabolomics investigation revealed that there are distinct differences among the compound patterns of Baihaoyinzhen (BHYZ) and Baimudan (BMD) white teas with various storage durations. The levels of flavan-3-ols, procyanidins, theasinensins, theaflavins, flavonol-O-glycosides, flavone-C-glycosides, and most of the amino acids were reduced after long-term (>4 years) storage. More importantly, 8-C N-ethyl-2-pyrrolidinone substituted flavan-3-ols (EPSFs), including 7 novel compounds discovered in white teas for the first time, were formed from theanine and flavan-3-ols during storage, and their contents were positively correlated with the storage duration. These findings were further confirmed by the linearly increasing formation of EPSFs in reaction solution and in BMD white teas stored in an environment-controlled cabinet. In conclusion, EPSFs were detected in white teas for the first time and were discovered as marker compounds and potential indicators for long-term storage of white tea.

    更新日期:2018-06-20
  • Ellagitannins from pomegranate ameliorates 5-fluorouracil-induced intestinal mucositis in rats while enhancing its chemotoxicity against HT-29 colorectal cancer cells through intrinsic apoptosis induction
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-06-19
    Xiao-Xin Chen, Kar Ho Lam, Yibin Feng, Kai Xu, Stephen C.W. Sze, Chi Wai Tang, George P.H. Leung, Calvin Kai-Fai Lee, Jun Shi, Zhijun Yang, Sheng-Tao Li, Zhang-Jin Zhang, Yanbo Zhang

    Worldwide, colorectal cancer (CRC) is a deleterious disease causing millions of death annually. 5-Fluorouracil (5-FU) is a first-line chemotherapy for CRC, but chemoresistance and gastrointestinal mucositis limit its efficacy. Polyphenol-rich foods are increasingly popular due to their potential beneficial role in cancer. Ellagitannins is a group of phenolic compounds commonly found in pomegranate, strawberries, raspberries, etc. The objective of this study was to explore whether ellagitannins from pomegranate (PETs) could ameliorate 5-FU-induced intestinal mucositis and enhance its efficacy against CRC. The results showed that PETs (100 mg/kg) counteracted 5-FU-induced intestinal mucositis in rats. The number of apoptotic cells per crypt was reduced from 1.50±0.21 to 0.85±0.18 (P<0.05). Moreover, PETs induced HT-29 CRC cell death through intrinsic apoptosis as demonstrated by dissipation of mitochondrial membrane potential, increased Bax to Bcl-2 ratio, and cleavage of caspase 9 and caspase 3. PETs and 5-FU combination treatments exhibited synergistic cytotoxicity against HT-29 cells with a weighted combination index of 0.3494. PETs (80 µg/mL) and 5-FU (40 µg/mL) treatments for 48 h induced 14.03±0.76% and 16.42±1.15% of HT-29 cells to undergo apoptosis while the combination treatment further increased apoptosis cells to 34.00±1.54% (P<0.05). Combination treatment of the cells also enhanced S phase cell cycle arrest as compared with PETs or 5-FU monotherapy (P<0.05). These results suggest that dietary ellagitannins from pomegranate could alleviate intestinal mucositis in rats induced by 5-FU while enhancing its toxicity against HT-29 cells through potentiation of apoptosis and cell cycle arrest.

    更新日期:2018-06-20
  • Control of α-Lactalbumin Aggregation by Modulation of Temperature and Concentration of Calcium and Cysteine
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-06-19
    Line Ravn Nielsen, Søren Bang Nielsen, ZICHEN ZHAO, Karsten Olsen, Jacob Holm Nielsen, Marianne N. Lund

    The effect of free cysteine (in different concentrations) on the thermal aggregation of calcium saturated (Ca-sat) and depleted (Ca-dep) α-lactalbumin (α-LA) was investigated at 25 °C, 50 °C, and 70 °C. The temperatures were chosen to be below denaturation temperature (Td) of Ca-dep and Ca-sat α-LA (25 °C), above Td of Ca-dep α-LA and below that of Ca-sat α-LA (50 °C), and above Td of Ca-sat α-LA (70 °C). Size exclusion chromatography coupled to multi-angle light scattering showed that no or only minor aggregation was obtained at the investigated temperatures for both Ca-dep and Ca-sat α-LA even at extended holding times. Aggregates of Ca-sat α-LA were larger than those developed for Ca-dep α-LA. The addition of cysteine, a low molecular mass free thiol, resulted in increased aggregation of both Ca-sat and Ca-dep α-LA. Comparison between SDS-PAGE run under reducing and non-reducing conditions showed that the nature of the formed cross-links was primarily disulfide bonds, but Western blot also showed small contributions from di-tyrosine cross-link formation. The aggregation kinetics related to monomer loss during heat treatment were determined by RP-UPLC and showed the addition of cysteine increased the rate of aggregation. Activation energies for the Ca-dep α-LA added 0.35 mM and 0.7 mM cysteine were found to be 59 ± 1 kJ/mol and 46 ± 4 kJ/mol, respectively, showing that less energy was needed for enhanced thermal aggregation of α-LA when the cysteine concentration was increased. This study showed that it was possible to control the aggregation size of α-LA by manipulating the incubation temperature and the cysteine concentration.

    更新日期:2018-06-19
  • Isolation and Characterization of Angiotensin I-Converting Enzyme (ACE) Inhibitory Peptides from the Enzymatic Hydrolysate of Carapax Trionycis (the shell of the turtle Pelodiscus sinensis)
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-06-19
    Pengying Liao, Xiongdiao Lan, Dankui Liao, LiXia Sun, Liqin Zhou, Jianhua Sun, Zhangfa Tong

    Carapax Trionycis (the shell of the turtle Pelodiscus sinensis) was hydrolyzed by six different commercial proteases. The hydrolysate prepared from papain showed stronger inhibitory activity against angiotensin I-converting enzyme (ACE) than other extracts. Two noncompetitive ACE inhibitory peptides were purified successively by ultrafiltration, gel filtration chromatography, ion exchange column chromatography, and high-performance liquid chromatography (HPLC). The amino acid sequences of them were identified as KRER and LHMFK, with IC50 values of 324.1 and 75.6 μM, respectively, confirming that Carapax Trionycis is a potential source of active peptides possessing ACE inhibitory activities. Besides, both enzyme kinetics and isothermal titration calorimetry (ITC) assay showed that LHMFK could form more stable complex with ACE than KRER, which is in accordance with the better inhibitory activity of LHMFK.

    更新日期:2018-06-19
  • Involvement of Three CsRHM Genes from Camellia sinensis in UDP-rhamnose Biosynthesis
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-06-19
    Xinlong Dai, Guifu Zhao, Tianming Jiao, Yingling Wu, Xinmin Li, Kan Zhou, Liping Gao, Tao Xia

    Abstract UDP-rhamnose synthase (RHM), the branch-point enzyme controlling the nucleotide sugar interconversion pathway, converts UDP-D-glucose into UDP-rhamnose. As a rhamnose residue donor, UDP-L-rhamnose is essential for the biosynthesis of pectic polysaccharides and secondary metabolites in plants. In this study, three CsRHM genes from tea plants (Camellia sinensis) were cloned and characterized. Enzyme assays showed that three recombinant proteins displayed RHM activity and were involved in the biosynthesis of UDP-rhamnose in vitro. The transcript profiles, metabolite profiles, and mucilage location suggest that the three CsRHM genes likely contribute to UDP-rhamnose biosynthesis and may be involved in primary wall formation in C. sinensis. These analyses of CsRHM genes and metabolite profiles provide a comprehensive understanding of secondary metabolite biosynthesis and regulation in tea plants. Moreover, our results can be applied for the synthesis of the secondary metabolite rhamnoside in future studies. Keywords: UDP-rhamnose synthase, Camellia sinensis, recombinant protein, enzyme assays, expression analysis

    更新日期:2018-06-19
  • Lauric Acid Accelerates Glycolytic Muscle Fiber Formation through TLR4 Signaling
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-06-18
    Leshan Wang, Lv Luo, Weijie Zhao, Kelin Yang, Gang Shu, Songbo Wang, Ping Gao, Xiaotong Zhu, Qianyun Xi, Yongliang Zhang, Qingyan Jiang, Lina Wang
    更新日期:2018-06-18
  • Transcriptomic Analysis of Red-fleshed Apples Reveals the Novel Role of MdWRKY11 in Flavonoid and Anthocyanin Biosynthesis
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-06-18
    Nan Wang, Wenjun Liu, Tianliang Zhang, Shenghui Jiang, Haifeng Xu, Yicheng Wang, Zongying Zhang, Chuanzeng Wang, Xuesen Chen

    In plants, flavonoids are important secondary metabolites that contribute to the nutritional quality of many foods. Apple is a popular and frequently consumed food because of its high flavonoid content. In this study, flavonoid composition and content were detected and compared between the red- and white-fleshed apples in a BC1 hybrid populations using ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry. Transcriptomic analysis of the red- and white-fleshed apples was then performed using RNA-seq technology. By screening differentially expressed genes encoding transcription factors we unearthed a WRKY family transcription factor designated MdWRKY11. Overexpression of MdWRKY11 promoted the expression of F3H, FLS, DFR, ANS and UFGT and increased the accumulation of flavonoids and anthocyanin in apple calli. Our findings explored novel role of MdWRKY11 in flavonoid biosynthesis and suggest several other genes that may be also potentially involved. This provides valuable information on flavonoid synthesis for the breeding of elite red-fleshed apples.

    更新日期:2018-06-18
  • Bioaccessibility during in vitro digestion and antiproliferative effect of bioactive compounds from Andean berry (Vaccinium meridionale Swartz) juice
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-06-18
    Carlos Daniel Agudelo, Ivan Luzardo-Ocampo, Rocio Campos-Vega, Guadalupe Loarca-Piña, Maria Maldonado

    Berries consumption is associated with colorectal cancer chemoprevention, but digestive conditions can affect this property. The bioaccessibility and apparent permeability coefficients of bioactive compounds from Andean berry juice (ABJ) after in vitro gastrointestinal digestion and colonic fermentation were analyzed. The antiproliferative effect of the fermented non-digestible fraction was evaluated against SW480 colon adenocarcinoma cells. Gallic acid displayed the highest bioaccessibility in the mouth, stomach, small intestine and colon. However, chlorogenic acid exhibited the highest apparent permeability coefficients (up to 1.98 x 10-4 cm/s). The colonic fermented fraction showed an increase of ≥ 50% antiproliferative activity against SW480 cells (19.32% v/v), equivalent to gallic acid: 13.04 µg/g; chlorogenic acid: 7.07 µg/g; caffeic acid: 0.40 µg/g; ellagic acid: 7.32 µg/g; rutin: 6.50 µg/g; raffinose: 0.14 mg/g; stachyose: 0.70 mg/g and xylose: 9.41 mg/g. Bioactive compounds from ABJ are bioaccessible through the gastrointestinal tract and colon fermentation, resulting in antiproliferative activity.

    更新日期:2018-06-18
  • Synthesis of Novel 3,4-Chloroisothiazole-Based Imidazoles as Fungicides and Evaluation of Their Mode of Action
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-06-18
    Lai Chen, Bin Zhao, Zhijin Fan, Xiumei Liu, Qifan Wu, Hongpeng Li, Haixia Wang

    A molecular design approach was used in our laboratory to guide the development of imidazole-based fungicides. Based on homology modeling and molecular docking studies targeting the cytochrome P450-dependent sterol 14α-demethylase, 3,4-dichloroisothiazole-based imidazoles showed great potential. Several such compounds were then rationally designed, synthesized, characterized, and their antifungal activities were evaluated. Bioassay results showed that compounds such as (R)-11, (R)-12, and (S)-11 have commendable, broad-spectrum antifungal activities that are comparable to those of commercial products. Based on Q-PCR testing and microscopy observations, the imidazole derivatives affect fungal cell wall formation through the inhibition of the BcCYP51 expression system. These findings strongly suggest that the mode of action of these imidazole compounds is similar to that of tioconazole and imazalil. This report indicates that this molecular design strategy is not only practical but productive.

    更新日期:2018-06-18
  • Human milk oligosaccharides in colostrum and mature milk of Chinese mothers: Lewis positive secretor subgroups
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-06-16
    Moheb Elwakiel, Jos Hageman, Wendan Wang, Ignatius Szeto, Hans van Goudoever, Kasper Arthur Hettinga, Henk A. Schols

    To study the variability in human milk oligosaccharide (HMO) composition of Chinese human milk over a 20-wk lactation period, HMO profiles of 30 mothers were analyzed using CE-LIF. This study showed that total HMO concentrations in Chinese human milk decreased significantly over a 20-wk lactation period, independent of the mother’s SeLe status, although with individual variations. In addition, total acidic and neutral HMO concentrations in Chinese human milk decreased over lactation, and levels are driven by their mother’s SeLe status. Analysis showed that total neutral fucosylated HMO concentrations in Chinese human milk were higher in the two secretor groups compared to the non-secretor group. Based on the total neutral fucosylated HMO concentrations in Chinese human milk, HMO profiles within the Se+Le+ group can be divided in 2 subgroups. HMOs that differed in level between Se+Le+ subgroups were 2’FL, DF-L, LNFP I, and F-LNO. HMO profiles in Dutch human milk also showed Se+Le+ subgroup division, with 2’FL, LNT, and F-LNO as driving force.

    更新日期:2018-06-17
  • Analytical Capabilities of the Community Bureau of Reference Protocol to Estimate the Mobility of Nutrients and Toxic Elements from Mineral Fertilizer
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-06-15
    Alexandre M. Fioroto, Luiza G. R. Albuquerque, Gislayne A. R. Kelmer, Delmarcio G. Silva, Ricardo A. A. Couto, Pedro V. Oliveira
    更新日期:2018-06-16
  • Alterations in the Gut (Gallus gallus) Microbiota Following the Consumption of Zinc Biofortified Wheat (Triticum aestivum)-Based Diet
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-06-15
    Spenser Reed, Marija Knez, Atara Uzan, James C. R. Stangoulis, Raymond P. Glahn, Omry Koren, Elad Tako
    更新日期:2018-06-16
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  • Cholesterol Content in Human Milk during Lactation: A Comparative Study of Enzymatic and Chromatographic Methods
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-06-15
    Islam J. A. Hamdan, Luis Manuel Sanchez-Siles, Esther Matencio, Guadalupe Garcia-Llatas, María Jesús Lagarda
    更新日期:2018-06-16
  • Cell Based-Green Fluorescent Biosensor using Cytotoxic Pathway for Bacterial Lipopolysaccharide Recognition
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-06-15
    Jiadi Sun, Pei Zhu, Xiumei Wang, Jian Ji, Jean de Dieu Habimana, Jingdong Shao, Hongtao Lei, Yinzhi Zhang, Xiulan Sun

    Lipopolysaccharide (LPS), a characteristic component of the outer membrane of Gram-negative bacteria, can be used as an effective biomarker to detect bacterial contamination. Here, we reported a 293/hTLR4A-MD2-CD14 cell-based fluorescent biosensor to detect and identify LPS, which is carried out in a 96-well microplate with nondestructive, user-friendly and high efficiency. The promoter sequence of the critical signaling pathway gene ZC3H12A (encoding MCPIP1 protein) and Enhanced Green Fluorescence Protein (EGFP) were combined to construct a recombinant plasmid, which was transferred into 293/hTLR4A-MD2-CD14 cells through lipid-mediated, DNA-transfection way. LPS was able to bind to TLR4 and co-receptors-induced signaling pathway could result in green fluorescent protein expression. Results show that stable transfected 293/hTLR4A-MD2-CD14 cells with LPS treatment could be directly and continually observed under High Content Screening imaging system. The novel cell-based biosensor detects LPS at low concentration, along with the detection limit of 0.075 μg/mL. The cell-based biosensor was evaluated by differentiating Gram-negative and Gram-positive bacteria and detecting LPS in fruit juices as well. This proposed fluorescent biosensor has potential in sensing LPS optically in foodstuff and biological products, as well as bacteria identification, contributing to the control of foodborne diseases and ensurance of public food safety with its high throughput detection way.

    更新日期:2018-06-16
  • Comparative Metabolomic and Proteomic Analyses Reveal the Regulation Mechanism Underlying MeJA-Induced Bioactive Compound Accumulation in Cutleaf Groundcherry (Physalis angulata L.) Hairy Roots
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-06-15
    Xiaori Zhan, Xinyue Liao, Xiujun Luo, Yujia Zhu, Shangguo Feng, Chunna Yu, Jiangjie Lu, Chenjia Shen, Huizhong Wang
    更新日期:2018-06-15
  • Metabolomics Profiling of Serum and Urine in Three Beef Cattle Breeds Revealed Different Levels of Tolerance to Heat Stress
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-06-15
    Yupeng Liao, Rui Hu, Zhisheng Wang, Quanhui Peng, Xianwen Dong, Xiangfei Zhang, Huawei Zou, Qijian Pu, Bai Xue, Lizhi Wang

    This study was to determine differences in the global metabolic profiles of serum and urine of Xuanhan yellow cattle, Simmental crossbred cattle (Simmental × Xuanhan yellow cattle) and cattle-yaks (Jersey × Maiwa yak) under heat stress (temperature-humidity index remained above 80 for 1 week). A total of 55 different metabolites associated with the three breeds were identified in the serum and urine samples by gas chromatography-mass spectrometry. The metabolic adaptations to heat stress are heterogeneous. Cattle-yaks mobilize a greater amount of body protein to release glucogenic amino acids to supply energy, whereas the tricarboxylic acid cycle is inhibited. Simmental crossbred cattle mobilize a greater amount of body fat to use free fatty acids as an energy source. In comparison with Simmental crossbred cattle and cattle-yaks, Xuanhan yellow cattle have higher glycolytic activity, and possess a stronger antioxidant defense system and are, in conclusion, more adapted to hot and humid environments.

    更新日期:2018-06-15
  • Differential effects of quercetin and its two derivatives (isorhamnetin and isorhamnetin-3- glucuronide) in inhibiting proliferation of human breast cancer MCF-7 cells
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-06-15
    Qiu Wu, Paul A. Kroon, Hongjun Shao, Paul W. Needs, Xingbin Yang

    Quercetin (Que) has consistently been reported to be useful cytotoxic compound in vivo and in vitro, but little is known on its metabolites. Here we examined and compared cytotoxic effect of Que and its water-soluble metabolites, isorhamnetin (IS) and isorhamnetin-3-glucuronide (I3G) in human breast cancer MCF-7 cells, and explain their tumor-inhibitory mechanism and structure-function relationship. The results showed that Que, IS and I3G could dose-dependently inhibit the growth of MCF-7 cells, and the cytotoxic effect was ranked as Que > IS > I3G. Furthermore, Que, IS and I3G mediated the cell-cycle arrest principally in S phase, followed by the decrease in the number of G0/G1 and G2/M, and 70.8%, 68.9% and 49.8% MCF-7 tumor cells entered early phase apotosis when treated with 100 µM Que, IS and I3G for 48 h, respectively. Moreover, induction of apoptosis by Que, IS and I3G were accompanied with the marginal generation of intracellular ROS. Given these results, Que, IS and I3G possess strong cytotoxic effect through a ROS-dependent apoptosis pathway in MCF-7 cells.

    更新日期:2018-06-15
  • Visual Assay of Glutathione in Vegetables and Fruits Using Quantum Dot Ratiometric Hybrid Probes
    J. Agric. Food Chem. (IF 3.154) Pub Date : 2018-06-14
    Aimin Chen, Xiao Peng, Zaifa Pan, Kang Shao, Jing Wang, Maohong Fan
    更新日期:2018-06-15
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Some contents have been Reproduced with permission of the American Chemical Society.
Some contents have been Reproduced by permission of The Royal Society of Chemistry.
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