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  • Identification of Missing Proteins in Normal Human Cerebrospinal Fluid
    J. Proteome Res. (IF 3.95) Pub Date : 2018-08-17
    Charlotte Macron, Lydie Lane, Antonio Núñez Galindo, Loïc Dayon
    更新日期:2018-08-18
  • Highly Efficient Phosphoproteome Capture and Analysis from Urinary Extracellular Vesicles
    J. Proteome Res. (IF 3.95) Pub Date : 2018-08-17
    Xiaofeng Wu, Li Li, Anton Iliuk, W. Andy Tao
    更新日期:2018-08-18
  • 更新日期:2018-08-17
  • Quantitative N-Terminal Footprinting of Pathogenic Mycobacteria Reveals Differential Protein Acetylation
    J. Proteome Res. (IF 3.95) Pub Date : 2018-08-16
    Cristal Reyna Thompson, Matthew M. Champion, Patricia A. Champion
    更新日期:2018-08-17
  • Spectral Library Search Improves Assignment of TMT Labeled MS/MS Spectra
    J. Proteome Res. (IF 3.95) Pub Date : 2018-08-16
    Jianqiao Shen, Vishwajeeth R. Pagala, Alex M. Breuer, Junmin Peng, Bin Ma, Xusheng Wang
    更新日期:2018-08-17
  • Gingival exudatome dynamics implicate inhibition of the alternative complement pathway in the protective action of the C3 inhibitor Cp40 in non-human primate periodontitis
    J. Proteome Res. (IF 3.95) Pub Date : 2018-08-16
    Nagihan Bostanci, Kai Bao, Li Xiaofei, Tomoki Maekawa, Jonas Grossmann, Christian Panse, Ruel A. Briones, Ranillo R.G Resuello, Joel V. Tuplano, Cristina A.G Garcia, Edimara S. Reis, John D. Lambris, George Hajishengallis

    Background and Aim: Periodontitis is a prevalent chronic inflammatory disease associated with dysbiosis. Although complement inhibition has been successfully used to treat periodontitis in animal models, studies globally analysing inflamed tissue proteins to glean insight into possible mechanisms of action are missing. Using quantitative shotgun proteomics, we aimed to investigate differences in composition of inflammatory gingival tissue exudate (‘gingival crevicular fluid’; GCF), before and after local administration of an inhibitor of the central complement component, C3, in non-human primates. Methods: The C3 inhibitor, Cp40 (also known as AMY-101) was administered locally in the maxillary gingival tissue of cynomolgus monkeys with established periodontitis, either once a week (‘1x treatment’; n=5 animals) or three times per week (‘3x-treatment’; n=10 animals), for six weeks followed by another six weeks of observation in the absence of treatment. 45 GCF samples were processed for FASP digestion and liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. Data were processed using the ProgenesisQI software. The statistical significance of differences between the groups was determined by RM-ANOVA and a protein expression change was considered as a true regulation at >2-fold and p<0.05. The human orthologues were subjected to Gene Ontology analyses using Panther. Data are available via ProteomeXchange with identifier PXD009502. Results: 573 proteins with >2 peptides were longitudinally quantified. Both 3x and 1x administration of Cp40 resulted in significant down-regulation of dozens of proteins during the 6-week course of treatment as compared to baseline. Following drug withdrawal at 6 weeks, more than 50% of the down-regulated proteins showed increased levels at week 12. The top scored pathway was ‘complement activation, alternative pathway’ and several proteins involved in this pathway were down-regulated at 6-weeks. Conclusion: We mapped the proteomic fingerprint changes in local tissue exudate of cynomolgus monkey periodontitis in response to C3 inhibition and identified the alternative pathway of complement activation and leukocyte degranulation as main targets, which are thus likely to play significant roles in periodontal disease pathogenesis. Label-free quantitative proteomics strategies utilizing GCF are powerful tools for the identification of treatment targets and providing insights into disease mechanisms.

    更新日期:2018-08-17
  • An integrated, high-throughput strategy for multi-omic systems level analysis
    J. Proteome Res. (IF 3.95) Pub Date : 2018-08-16
    Danielle B. Gutierrez, Randi L Gant-Branum, Carrie E Romer, Melissa A Farrow, Jamie L Allen, Nikesh Dahal, Yuan-Wei Nei, Simona G Codreanu, Ashley T Jordan, Lauren D Palmer, Stacy D. Sherrod, John A. McLean, Eric P Skaar, Jeremy L Norris, Richard M. Caprioli

    Proteomics, metabolomics, and transcriptomics generate comprehensive datasets, and current biocomputational capabilities allow their efficient integration for systems biology analysis. Published multi-omics studies cover methodological advances as well as applications to biological questions. However, few studies have focused on the development of a high-throughput, unified sample preparation approach to complement high-throughput omic analytics. This report details the automation, benchmarking, and application of a strategy for transcriptomic, proteomic, and metabolomic analyses from a common sample. The approach, Sample Preparation for multi-Omics Technologies (SPOT), provides equivalent performance to typical individual omic preparation methods, but it greatly enhances throughput and minimizes the resources required for multi-omic experiments. SPOT was applied to a multi-omics time course experiment for zinc-treated HL60 cells. The data reveal Zn effects on NRF2 antioxidant and NFkappaB signaling. High-throughput approaches such as these are critical for the acquisition of temporally resolved, multi-condition, large multi-omic datasets, such as those necessary to assess complex clinical and biological concerns. Ultimately, this type of approach will provide an expanded understanding of challenging scientific questions across many fields.

    更新日期:2018-08-17
  • Development of RBC membrane antigen arrays for validating Blood Grouping Reagents
    J. Proteome Res. (IF 3.95) Pub Date : 2018-08-16
    Lu Yang, Yang Yu, Chunya Ma, Hongye Wang, Jiayu Dai, Hu Duan, Zhonglin Fu, Ping Wu, Deqing Wang, Xiaobo Yu

    Antibody reagents have been remained as a standard approach to characterize blood group (BG) antigens in clinic. The specificity and cross-reactivity of these BG antibodies are routine detected using the gel microcolumn assay (GMA). However, the GMA is neither specific nor sensitive, thus increasing the risk of improperly-matched RBC transfusions. In this work, we describe a bead-based RBC membrane antigen array to detect BG antibody-antigen binding with ~700-fold higher sensitivity and dynamic range than the GMA. RBC membrane antigen arrays were fabricated using fragmented RBC membranes highly enriched in BG panel antigens. The arrays were then used to screen the interactions of 15 BG reagents to three antigen panels. The majority of the antibody reactions (i.e., 86.7%; 39/45) aligned with those obtained with the GMA. The six cross-reactive, non-specific antibody reactions identified only by our arrays (i.e., 13.3%; 6/45) were confirmed by agglutination inhibition and genotyping assays. These results demonstrate that our RBC membrane antigen array has great potential in screening BG antibodies and improving the safety of RBC transfusions.

    更新日期:2018-08-17
  • Identification of Missing Proteins in Human Olfactory Epithelial Tissue by Liquid Chromatography-Tandem Mass Spectrometry
    J. Proteome Res. (IF 3.95) Pub Date : 2018-08-16
    Heeyoun Hwang, Ji Eun Jeong, Hyun Kyoung Lee, Ki Na Yun, Hyun Joo An, Bonghee Lee, Young-Ki Paik, Tae Seok Jeong, Gi Taek Yee, Jin Young Kim, Jong Shin Yoo

    We performed proteomic analyses of human olfactory epithelial tissue to identify missing proteins using liquid chromatography-tandem mass spectrometry. Using a next-generation proteomic pipeline with a <1.0% false discovery rate at the peptide and protein levels, we identified 3,731 proteins, among which five were missing proteins (P0C7M7, P46721, P59826, Q658L1, and Q8N434). We validated the identified missing proteins using the corresponding synthetic peptides. No olfactory receptor (OR) proteins were detected in olfactory tissue, suggesting that detection of ORs would be very difficult. We also identified 49 and 50 alternative splicing variants mapped at the neXtProt and GENCODE databases, respectively, and 2,000 additional single amino acid variants. This dataset is available at the ProteomeXchange consortium via PRIDE repository (PXD010025, Username: reviewer36079@ebi.ac.uk, Password: 9qPYFY3e).

    更新日期:2018-08-17
  • Proteomic Analysis of Urine from California Sea Lions (Zalophus californianus): a Resource for Urinary Biomarker Discovery
    J. Proteome Res. (IF 3.95) Pub Date : 2018-08-16
    Benjamin A. Neely, Katherine Prager, Alison M Bland, Christine Fontaine, Frances M. Gulland, Michael G. Janech

    Urinary markers for the assessment of kidney diseases in wild animals are limited, in part, due to the lack of urinary proteome data, especially for marine mammals. One of the most prevalent kidney diseases in marine mammals is caused by Leptospira interrogans, which is the second most common etiology linked to stranding of California sea lions (Zalophus californianus). Urine proteins from eleven sea lions with leptospirosis kidney disease and eight sea lions without leptospirosis or kidney disease were analyzed using shotgun proteomics. In total, 2694 protein groups were identified and 316 were differentially abundant between groups. Major urine proteins in sea lions were similar to major urine proteins in dogs and humans except for the preponderance of resistin, lysozyme C, and PDZ domain containing 1, which appear to be over-represented. Previously reported urine protein markers of kidney injury in humans and animals were also identified. Notably, neutrophil gelatinase-associated lipocalin, osteopontin, and epidermal fatty acid binding protein were elevated over 20-fold in the leptospirosis-infected sea lions. Consistent with leptospirosis infection in rodents, urinary proteins associated with the renin-angiotensin system were depressed, including neprilysin. This study represents a foundation from which to explore the clinical use of urinary protein markers in California sea lions.

    更新日期:2018-08-17
  • S-Trap is an ultrafast sample preparation approach for shotgun proteomics
    J. Proteome Res. (IF 3.95) Pub Date : 2018-08-16
    Milkessa HaileMariam, Rodrigo Vargas Eguez, Harinder Singh, Shiferaw Bekele, Gobena Ameni, Rembert Pieper, Yanbao Yu

    The success of shotgun proteomic analysis depends largely on how samples are prepared. Current approaches such as gel-, solution- or filter-based, although being extensively employed in the field, are time-consuming and less effective with respect to the repetitive sample processing, recovery, and overall yield. As an alternative, the suspension trapping (S-Trap) filter is commercially available very recently in the format of single or 96-well filter plate. In contrast to the conventional filter aided sample preparation (FASP) approach, which utilizes a molecular weight cutoff (MWCO) membrane as the filter and requires hours of processing before digestion-ready proteins can be obtained, S-Trap employs a three-dimensional porous material as filter media, and traps particulate protein suspension with subsequent depletion of interfering substances and in-filter digestion. Due to the large (sub-micron) pore size, each centrifugation cycle of the S-Trap filter only takes one minute, which significantly reduces the total processing time from approximately three hours by FASP to less than 15 minutes, suggesting an ultrafast sample preparation approach for shotgun proteomics. Here, we comprehensively evaluate the performance of the individual S-Trap filter and 96-well filter plate in the context of global protein identification and quantitation using whole cell lysate and clinically relevant sputum samples.

    更新日期:2018-08-17
  • N-linked glycan branching and fucosylation are increased directly in hepatocellular carcinoma tissue as determined through in situ glycan imaging
    J. Proteome Res. (IF 3.95) Pub Date : 2018-08-15
    Connor A. West, Mengjun Wang, Harmin Herrera, Hongyan Liang, Alyson Black, Peggi M. Angel, Richard R. Drake, Anand S. Mehta

    Hepatocellular carcinoma (HCC) remains as the fifth most common cancer in the world and accounts for more than 700,000 deaths annually. Changes in serum glycosylation have long been associated with this cancer but the source of that material is unknown and direct glycan analysis of HCC tissues has been limited. Our laboratory previously developed a method of in situ tissue based N-linked glycan imaging that bypasses the need for microdissection and solubilization of tissue prior to analysis. We used this methodology in the analysis of 138 HCC tissue samples and compared the N-linked glycans in cancer tissue with either adjacent untransformed or tissue from patients with liver cirrhosis but no cancer. Ten glycans were found significantly elevated in most HCC tissues as compared to cirrhotic or adjacent tissue. These glycans fell into two major classes, those with increased levels of fucosylation and those with increased levels of branching without any fucose modifications. In addition, increased levels of fucosylated glycoforms were associated with a reduction in survival time. This work supports the hypothesis that the increased levels of fucosylated N-linked glycans in HCC serum are produced directly from the cancer tissue.

    更新日期:2018-08-16
  • Proteomics goes to court: A statistical foundation for forensic toxin/organism identification using bottom-up proteomics
    J. Proteome Res. (IF 3.95) Pub Date : 2018-08-15
    Kristin H Jarman, Natalie C Heller, Sarah C Jenson, Janine R. Hutchinson, Brooke L Deatherage Kaiser, Samuel H. Payne, David S. Wunschel, Eric D. Merkley

    Bottom-up proteomics is increasingly being used to characterize unknown environmental, clinical and forensic samples. Proteomics-based bacterial identification typically proceeds by tabulating peptide “hits” (i.e. confidently identified peptides) associated with the organisms in a database; those organisms with enough hits are declared present in the sample. This approach has proven successful in laboratory studies, however, important research gaps remain. First, the common-practice reliance on unique peptides for identification is susceptible to a phenomenon known as signal erosion. Second, no general guidelines are available for determining how many hits are needed to make a confident identification. These gaps inhibit the transition of this approach to real-world forensic samples where conditions vary and large databases may be needed. In this work, we propose statistical criteria that overcome the problem of signal erosion and can be applied regardless of sample quality or data analysis pipeline. These criteria are straightforward, producing a p-value on the result of an organism or toxin identification. We test the proposed criteria on 919 LC-MS/MS datasets originating from two toxins and 32 bacterial strains acquired using multiple data collection platforms. Results reveal a >95% correct species-level identification rate, demonstrating the effectiveness and robustness of proteomics-based organism/toxin identification.

    更新日期:2018-08-15
  • Global analysis of lysine 2-hydroxyisobutyrylome upon SAHA treatment and its relationship with acetylation and crotonylation
    J. Proteome Res. (IF 3.95) Pub Date : 2018-08-15
    Quan Wu, Li Ke, Chi Wang, Pingsheng Fan, Zhiwei Wu, Xiaoling Xu

    Lysine 2-hydroxyisobutyrylation is a newly discovered protein acylation and was reported to share acyltransferases and deacylases with the widely studied lysine acetylation. The well-known acetyltransferase Tip60 and histone deacetylases HDAC 2 and HDAC 3 were discovered to be “writer” and “eraser” of this new PTM on histones. However, the acyltransferases and deacylases for non-histone proteins are still unclear. In this work, lysine 2-hydroxyisobutyrylome on both histones and non-histone proteins upon SAHA treatment were intensively studied and 8,765 lysine 2-hydroxyisobutyrylation sites on 2,484 proteins were identified in A549 cells. This is the largest dataset of lysine 2-hydroxyisobutyrylome in mammalian cells by now. It was found that lysine 2-hydroxyisobutyrylation participate in varieties of biological functions and processes including ribosome, glycolysis/gluconeogenesis and transcription. More importantly, it was found that most quantified sites on core histones were up-regulated upon SAHA treatment for all of 2-hydroxyisobutyrylation, crotonylation and acetylation and the fold changes upon SAHA of 2-hydroxyisobutyrylation and crotonylation on non-histone proteins were highly correlated, while their fold changes have little correlations with acetylation on non-histone proteins.

    更新日期:2018-08-15
  • Higher Energy Collisional Dissociation Mass Spectrometry of Sulfated O-Linked Oligosaccharides
    J. Proteome Res. (IF 3.95) Pub Date : 2018-08-13
    Samah M. A. Issa, Varvara Vitiazeva, Catherine A. Hayes, Niclas G. Karlsson
    更新日期:2018-08-14
  • ‘Candidatus Liberibacter asiaticus’ minimally alters expression of immunity and metabolism proteins in the hemolymph of Diaphorina citri, the insect vector of Huanglongbing.
    J. Proteome Res. (IF 3.95) Pub Date : 2018-08-14
    Angela Kruse, John S. Ramsey, Richard Johnson, David G. Hall, Michael J. MacCoss, Michelle Heck

    Huanglongbing (HLB), also known as citrus greening disease, is the most serious disease of citrus plants. It is associated with the Gram-negative bacterium ‘Candidatus Liberibacter asiaticus’ (CLas), which is transmitted between host plants by the hemipteran insect vector Diaphorina citri in a circulative, propagative manner involving specific interactions with various insect tissues, including the hemolymph, fluid that occupies the body cavity akin to insect blood. High resolution quantitative mass spectrometry was performed to investigate the effect of CLas exposure on D. citri hemolymph at the proteome level. In contrast to the broad proteome effects on hundreds of proteins and a diverse array of metabolic pathways previously reported in gut and whole insect proteome analyses, the effect of CLas on the hemolymph was observed to be highly specific, restricted to key immunity and metabolism pathways, and lower in magnitude than that previously observed in the whole insect body and gut. Vitellogenins were abundantly expressed and CLas-responsive. Gene-specific RNA expression analysis suggests that these proteins are expressed in both male and female insects, and may have roles outside of reproductive vitellogenesis. Proteins for fatty acid synthesis were found to be up-regulated, along with metabolic proteins associated with energy production, supported at the organismal level by the previously published observation that D. citri individuals experience a higher level of hunger when reared on CLas-infected plants. Prediction of post-translational modifications identified hemolymph proteins with phosphorylation and acetylation upon CLas exposure. Proteins derived from the three most prominent bacterial endosymbionts of the psyllid were also detected in the hemolymph, and several of these have predicted secretion signals. A DNAK protein, the bacterial HSP70, detected in the hemolymph expressed from Wolbachia pipientis was predicted to encode a eukaryotic nuclear localization signal. Taken together, these data show specific changes to immunity and metabolism in D. citri hemolymph involving host and endosymbiont proteins. These data provide a novel context for proteomic changes seen in other D. citri tissues in response to CLas and align with organismal data on the effects of CLas on D. citri metabolism and reproduction.

    更新日期:2018-08-14
  • KELM-CPPpred: Kernel Extreme Learning Machine Based Prediction Model for Cell-Penetrating Peptides
    J. Proteome Res. (IF 3.95) Pub Date : 2018-08-13
    Poonam Pandey, Vinal Patel, Nithin V. George, Sairam S. Mallajosyula
    更新日期:2018-08-13
  • Progress on Identifying and Characterizing the Human Proteome: 2018 Metrics from the HUPO Human Proteome Project
    J. Proteome Res. (IF 3.95) Pub Date : 2018-08-12
    Gilbert S Omenn, Lydie Lane, Christopher M Overall, Fernando J. Corrales, Jochen M Schwenk, Young-Ki Paik, Jennifer E. Van Eyk, Siqi Liu, Michael Snyder, Mark S. Baker, Eric W. Deutsch

    The Human Proteome Project (HPP) annually reports on progress throughout the field in credibly identifying and characterizing the human protein parts list and making proteomics an integral part of multi-omics studies in medicine and the life sciences. neXtProt release 2018-01-17, the baseline for this 6th annual HPP special issue of the Journal of Proteome Research, contains 17,470 PE1 proteins, 89% of all neXtProt predicted PE1-4 proteins, up from 17,008 in release 2017-01-23 and 13,975 in release 2012-02-24. Conversely, the number of neXtProt PE2,3,4 missing proteins has been reduced from 2949 to 2579 to 2186 over the past two years. Of the PE1 proteins, 16,092 are based on mass spectrometry results, and 1378 on other kinds of protein studies, notably protein-protein interaction findings. PeptideAtlas has 15,798 canonical proteins, up 625 over the past year, including 269 from SUMOylation studies. The largest reason for missing proteins is low abundance. Meanwhile, the Human Protein Atlas has released its Cell Atlas, Pathology Atlas, and updated Tissue Atlas, and is applying recommendations from the International Working Group on Antibody Validation. Finally, there is progress applying the quantitative multiplex organ-specific popular proteins targeted proteomics approach in various disease categories.

    更新日期:2018-08-13
  • Differential antibody responses to outer membrane proteins contribute to differential immune protections between live and inactivated Vibrio parahaemolyticus
    J. Proteome Res. (IF 3.95) Pub Date : 2018-08-10
    Xiang Liu, Manjun Yang, Shengnan Wang, Di Xu, Hui Li, Xuan-xian Peng

    It is widely accepted that live vaccines elicit higher immune protection than inactivated vaccines. However, the mechanisms are largely unknown. Here, an array with 64 recombinant outer membrane proteins of Vibrio parahaemolyticus was developed to explore antibody responses of live and inactivated V. parahaemolyticus post immunization of 8th, 12th, 16th and 20th day. Among the 64 outer membrane proteins, 28 elicited antibody generation. They were all detected in live vaccine-induced immunity, but only 15 antibodies were found in inactivated vaccine-induced immunity. Passive immunization showed that higher percent survival was detected in live than inactivated vaccine-induced immunities. Active immunization indicated that out of 19 randomly selected outer membrane proteins, 5 stimulated immune protection against V. parahaemolyticus infection. Among them, antibodies to VP2309 and VPA0526 shared in mice immunized by live or inactivated vaccines, while antibodies to VPA0548, VPA1745 and VP1667 only found in mice immunized by live vaccine. In addition, live V. parahaemolyticus stimulated earlier antibody response than inactivated bacteria. These results indicate that not all the outer membrane proteins elicited antibody responses when they work together in form of live or inactivated bacteria; live vaccine elicits more protective antibodies, which contribute to higher immune protection in live vaccine than inactivated vaccine. Notaly, the recombinant proteins might be different from those seperated from live bacteria, and they might be different in their immunogenic potencies.

    更新日期:2018-08-11
  • SIRT3 Overexpression Inhibits Growth of Kidney Tumor Cells and Enhances Mitochondrial Biogenesis
    J. Proteome Res. (IF 3.95) Pub Date : 2018-08-10
    Huan Liu, Siying Li, Xiaohui Liu, Yuling Chen, Haiteng Deng

    SIRT3 is a NAD+-dependent mitochondrial protein deacetylase implicated in the regulation of central metabolism and mitochondrial proteostasis. SIRT3 is downregulated in clear cell renal cell carcinoma (ccRCC), which is the most common form of renal cancer. Although ccRCC is characterized by a typical Warburg-like phenotype, mitochondrial dysfunction and elevated fat deposition, it is unknown whether SIRT3 plays a role in tumorigenesis and the development of this disease. In the present study, we found that SIRT3 overexpression and knockdown had opposing effects on the growth of ccRCC cells, decreasing and increasing the rate of cell proliferation, respectively. SIRT3 overexpression also increased mitochondrial mass in ccRCC cells. Unexpectedly, SIRT3 overexpression increased ROS levels, and sensitized cells to oxidative stress. Metabolomics and quantitative proteomics showed that SIRT3 overexpression alterd cellular metabolism and reversed the Warburg effect in ccRCC cells. Further studies demonstrated that SIRT3 promoted mitochondrial biogenesis by increasing both the expression and deacetylation of TFAM (transcription factor A, mitochondrial). Mutagenesis experiments revealed that acetylation of TFAM at K154 impaired TFAM interaction with mitochondrial DNA, thereby decreasing the activity of the protein and, consequently, mitochondrial biogenesis. Overall, our results suggest that SIRT3 regulates mitochondrial biogenesis and that its downregulation promotes a Warburg phenotype in ccRCC.

    更新日期:2018-08-11
  • NMR-based Serum Metabolomics of patients with Takayasu arteritis (TA) - Relationship with disease activity
    J. Proteome Res. (IF 3.95) Pub Date : 2018-08-10
    Avinash Jain, Dinesh Kumar, Anupam Guleria, Durga Prasanna Misra, Abhishek Zanwar, Smriti Chaurasia, Sandeep Kumar, Umesh Kumar, Shravan K. Mishra, Ruchika Goel, Debashish Danda, Ramnath Misra

    Takayasu arteritis (TA) is a large vessel vasculitis of unknown pathogenesis. Assessment of disease activity is a challenge and there is an unmet need for relevant biomarker(s). In our previous study, NMR based serum metabolomics had revealed distinctive metabolic signatures in TA patients compared with age/sex matched healthy controls and Systemic Lupus Erythematosus (SLE). In this study, we investigate whether the metabolites correlate with disease activity. Patients with TA fulfilling American College of Rheumatology (ACR) criteria were enrolled and disease activity was assessed using Indian Takayasu Clinical Activity Score using Acute Phase Reactant – Erythrocyte Sedimentation Rate [ITAS-A (ESR]. Sera were analysed using 800 MHz NMR spectrometer to identify metabolites [based on Partial Least Squares Discriminant Analysis (PLS-DA) VIP (variable importance in projection) score >1.0 and permutation test, p-value < 0.01]. 45 active and 53 inactive TA patients with median age 27 [(IQR) 22-35 years] and 27 [(IQR) 23-37 years] female to male ratio 3.5:1 and 4.9:1 and median duration of illness 5 [(IQR) 2-9 years] and 3 [(IQR) 1-6 years] years respectively were enrolled. The key metabolites with highest discriminatory potential in active TA (ITAS-A ≥4) were glutamate and N-acetyl glycoprotein (NAG), both elevated, with area under the curve 0.775 and 0.769 (p-value<0.001). On follow up assessment, metabolic spectra started to differ with change in disease activity. This large cohort of patients revealed metabolic profiles discriminating between clinically active and inactive TA patients. It suggests glutamate and NAG have strong potential as biomarkers for disease activity in TA and may serve as a guide to therapy. We are now working to further validate these results in longitudinal studies.

    更新日期:2018-08-11
  • Characterization of Naïve and Vitamin C-Treated Mouse Schwann Cell Line MSC80: Induction of the Antioxidative Thioredoxin Related Transmembrane Protein 1
    J. Proteome Res. (IF 3.95) Pub Date : 2018-08-10
    Vietxuan Phan, Jens Schmidt, Vitali Matyash, Sebastian Malchow, Michaela Thanisch, Christin Lorenz, Irmgard Diepolder, Jörg Bernhard Schulz, Werner Stenzel, Andreas Roos, Burkhard Gess
    更新日期:2018-08-10
  • Combined Enrichment/Enzymatic Approach To Study Tightly Clustered Multisite Phosphorylation on Ser-Rich Domains
    J. Proteome Res. (IF 3.95) Pub Date : 2018-08-09
    Evgeny Kanshin, Mirela Pascariu, Mike Tyers, Damien D’Amours, Pierre Thibault
    更新日期:2018-08-10
  • Crude-MS Strategy for in-Depth Proteome Analysis of the Methane-Oxidizing Methylocystis sp. strain SC2
    J. Proteome Res. (IF 3.95) Pub Date : 2018-08-08
    Anna Hakobyan, Werner Liesack, Timo Glatter
    更新日期:2018-08-10
  • A Cloud-Based Metabolite and Chemical Prioritization System for the Biology/Disease-driven Human Proteome Project
    J. Proteome Res. (IF 3.95) Pub Date : 2018-08-10
    Kun-Hsing Yu, Tsung-Lu Michael Lee, Yu-Ju Chen, Christopher Ré, Samuel C. Kou, Jung-Hsien Chiang, Michael Snyder, Isaac S. Kohane

    Targeted metabolomics and biochemical studies complement the ongoing investigations led by the Human Proteome Organization (HUPO) Biology/Disease-driven-Human Proteome Project (B/D-HPP). However, it is challenging to identify and prioritize metabolite and chemical targets. Literature mining-based approaches have been proposed for target proteomics studies, but text mining methods for metabolite and chemical prioritization is hindered by a large number of synonyms and non-standardized names of each entity. In this study, we developed a cloud-based literature mining and summarization platform that maps metabolites and chemicals in the literature to unique identifiers and summarizes the co-publication trends of metabolite/chemicals and B/D-HPP topics using the Protein Universal Reference Publication-Originated Search Engine (PURPOSE) scores. We successfully prioritized metabolites and chemicals associated with the B/D-HPP targeted fields, with the results validated by checking against expert-curated associations and enrichment analyses. Comparing with existing algorithms, our system achieved better precision and recall in retrieving chemicals related to B/D-HPP focused area. Our cloud-based platform enables queries on all biological terms in multiple species, which will contribute to B/D-HPP and targeted metabolomics/chemical studies.

    更新日期:2018-08-10
  • Simultaneous Extraction of RNA and Metabolites from Single Kidney Tissue Specimens for Combined Transcriptomic and Metabolomic Profiling
    J. Proteome Res. (IF 3.95) Pub Date : 2018-08-09
    Patrick Leuthold, Matthias Schwab, Ute Hofmann, Stefan Winter, Steffen Rausch, Michael N. Pollak, Jörg Hennenlotter, Jens Bedke, Elke Schaeffeler, Mathias Haag

    Tissue analysis represents a powerful tool for the investigation of disease pathophysiology. However, the heterogeneous nature of tissue samples, in particular of neoplastic, may affect the outcome of such analysis and hence obscure interpretation of results. Thus comprehensive isolation and extraction of transcripts and metabolites from an identical tissue specimen would minimize variations and enable the economic use of biopsy material which is usually available in limited amounts. Here we demonstrate a fast and simple protocol for combined transcriptomics and metabolomics analysis in homogenates prepared from one single tissue sample. Metabolites were recovered by protein precipitation from lysates originally prepared for RNA isolation and were analyzed by LC-QTOF-MS after HILIC and RPLC separation, respectively. Strikingly, although ion suppression was observed, over 80% of the 2885 detected metabolic features could be extracted and analyzed with high reproducibility (CV ≤ 20%). Moreover fold changes of different tumor and nontumor kidney tissues were correlated to an established metabolomics protocol and revealed a strong correlation (rp ≥ 0.75). In order to demonstrate the feasibility of the combined analysis of RNA and metabolites, the protocol was applied to kidney tissue of metformin treated mice to investigate drug induced alterations.

    更新日期:2018-08-10
  • Bradyrhizobium diazoefficiens USDA 110-Glycine max interactome provides candidate proteins associated with symbiosis
    J. Proteome Res. (IF 3.95) Pub Date : 2018-08-09
    Li Zhang, Jin-Yang Liu, Huan Gu, Yanfang Du, Jian-Fang Zuo, Zhibin Zhang, Menglin Zhang, Pan Li, Jim M Dunwell, Yangrong Cao, Zuxin Zhang, Yuan-Ming Zhang

    Although the legume-rhizobium symbiosis is a most important biological process, there is a limited knowledge about the protein interaction network between host and symbiont. Using interolog and domain-based approaches, we constructed an inter-species protein interactome containing 5,115 protein-protein interactions between 2,291 Glycine max and 290 Bradyrhizobium diazoefficiens USDA 110 proteins. The interactome was further validated by expression pattern analysis in nodules, GO term semantic similarity, co-expression analysis and luciferase complementation image assay. In G. max-B. diazoefficiens interactome, bacterial proteins are mainly ion channel and transporters of carbohydrates and cations, while G. max proteins are mainly involved in the processes of metabolism, signal transduction and transport. We also identified the top ten highly interacting proteins (hubs) for each species. KEGG pathway analysis for each hub showed that two 14-3-3 proteins (SGF14g and SGF14k) and five heat shock proteins in G. max are possibly involved in symbiosis, and ten hubs in B. diazoefficiens may be important symbiotic effectors. Subnetwork analysis showed that 18 symbiosis-related SNARE proteins may play roles in regulating bacterial ion channels, and SGF14g and SGF14k possibly regulate the rhizobium dicarboxylate transport protein DctA. The predicted interactome provide a valuable basis for understanding the molecular mechanism of nodulation in soybean.

    更新日期:2018-08-10
  • Ron receptor signaling ameliorates hepatic fibrosis in a diet-induced non-alcoholic steatohepatitis mouse model
    J. Proteome Res. (IF 3.95) Pub Date : 2018-08-09
    Joselyn Allen, Jingtao Zhang, Michael D. Quickel, Mary Kennett, Andrew D. Patterson, Pamela A. Hankey-Giblin

    Liver fibrosis is commonly observed in the terminal stages of non-alcoholic steatohepatitis (NASH) and with no specific and effective anti-fibrotic therapies available, this disease is a major global health burden. The MSP/Ron receptor axis has been shown to have anti-inflammatory properties in a number of mouse models, due at least in part, to its ability to limit pro-inflammatory responses in tissue-resident macrophages and hepatocytes. In this study, we established the role of the Ron receptor in steatohepatitis-induced hepatic fibrosis using Ron ligand domain knockout mice on an apolipoprotein E knockout background (DKO). After 18 weeks of high-fat high-cholesterol feeding, loss of Ron activation resulted in exacerbated NASH-associated steatosis which is precedent to hepatocellular injury, inflammation and fibrosis. 1H nuclear magnetic resonance (NMR)-based metabolomics identified significant changes in serum metabolites that can modulate the intrahepatic lipid pool in hepatic steatosis. Serum from DKO mice had higher concentrations of lipids, VLDL/LDL and pyruvate, whereas glycine levels were reduced. Parallel to the aggravated steatohepatitis, increased accumulation of collagen, inflammatory immune cells and collagen producing myofibroblasts were seen in the livers of DKO mice. Gene expression profiling revealed that DKO mice exhibited elevated expression of genes encoding Ron receptor ligand MSP, collagens, ECM remodeling proteins and pro-fibrogenic cytokines in the liver. Our results demonstrate the protective effects of Ron receptor activation on NASH-induced hepatic fibrosis.

    更新日期:2018-08-10
  • Triflic Acid Treatment Enables LC-MS/MS Analysis of Insoluble Bacterial Biomass
    J. Proteome Res. (IF 3.95) Pub Date : 2018-08-08
    Ana Y. Wang, Peter S. Thuy-Boun, Gregory S. Stupp, Andrew I. Su, Dennis W. Wolan
    更新日期:2018-08-08
  • Evaluating the possibility of detecting variants in shotgun proteomics via LeTE-fusion analysis pipeline
    J. Proteome Res. (IF 3.95) Pub Date : 2018-08-08
    Tung-Shing Mamie Lih, Wai-Kok Choong, Yu-Ju Chen, Ting-Yi Sung

    In proteogenomic studies, many genome-annotated events, e.g., single amino acid variation (SAAV) and short INDEL, are often unobserved in shotgun proteomics. Therefore, we propose an analysis pipeline called LeTE-fusion (Le: peptide length, T: theoretical values, E: experimental data) to first investigate whether peptides with certain lengths are observed more often in mass spectrometry (MS)-based proteomics, which may hinder peptide identification causing difficulty in detecting genome-annotated events. By applying LeTE-fusion on different MS-based proteome data sets, we found peptides within 7-20 amino acids are more frequently identified, possibly attributed to MS-related factors instead of proteases. We then further extended the usage of LeTE-fusion on four variant-containing-sequence data sets (SAAV-only) with various sample complexity up to the whole human proteome scale, which yields theoretically ~70% variants observable in an ideal shotgun proteomics. However, only ~40% of variants might be detectable in real shotgun proteomic experiments when LeTE-fusion utilizes the experimentally observed variant-site-containing wild-type peptides in PeptideAtlas to estimate the expected observable coverage of variants. Finally, we conducted a case study on HEK293 cell line with variants reported at genomic level that were also identified in shotgun proteomics to demonstrate the efficacy of LeTE-fusion on estimating expected observable coverage of variants. To the best of our knowledge, this is the first study to systematically investigate the detection limits of genome-annotated events via shotgun proteomics using such analysis pipeline.

    更新日期:2018-08-08
  • Inter-species developmental differences in metabonomic phenotypes of Lycium ruthenicum and L. barbarum fruits
    J. Proteome Res. (IF 3.95) Pub Date : 2018-08-07
    Qi Wang, Shaohua Zeng, Xiangyu Wu, Hehua Lei, Ying Wang, Huiru Tang

    Fruits of Lycium ruthenicum (LR) and L. barbarum (LB) in Solanaceae family contain abundant bioactive metabolites used widely as functional food and natural medicine. To characterize the fruit developmental molecular phenotypes, we comprehensively analyzed metabolite composition of both Lycium fruits at three developmental stages using the combined NMR, LC-MS/MS and GC-FID/MS methods. The metabonomes of these fruits were dominated by over 90 metabolites including sugars, amino acids, TCA cycle intermediates, fatty acids, choline metabolites and shikimate-mediated plant secondary metabolites. Metabolic phenotypes of two species differed significantly at all three developmental stages; LB fruits contain significantly more sugars and amino acids but less TCA cycle intermediates, fatty acids and secondary metabolites than LR. Interspecies differences for fatty acid levels were much greater after color-breaking than pre-color-breaking. Furthermore, LR fruits contained more osmolytes than LB fruits indicating different osmoregulation requirements for these fruits during development. Significant differences were also present in biosynthesis of shikimate-mediated plant secondary metabolites in LR and LB. These findings provided essential metabolic information for plant physiology of these Lycium species and their utilizations and demonstrating the usefulness of this metabonomic phenotyping approach for studying fundamental biochemistry of the plant development.

    更新日期:2018-08-08
  • Photosynthetic and Stress Responsive Proteins are Altered More Effectively in Nicotiana benthamiana Infected with Plum pox virus Aggressive PPV-CR Versus Mild PPV-C Cherry-Adapted Isolates
    J. Proteome Res. (IF 3.95) Pub Date : 2018-08-07
    Slavomira Novakova, Maksym Danchenko, Ludovit Skultety, Ivana Fialová, Alexandra Lešková, Gábor Beke, Gabriela Flores-Ramirez, Miroslav Glasa

    Plum pox virus (PPV, family Potyviridae) is one of the most important viral pathogens of Prunus spp. causing considerable damage to stone-fruit industry worldwide. Among the PPV strains identified so far, only PPV-C, PPV-CR, and PPV-CV are able to infect cherries under natural conditions. Herein, we evaluated the pathogenic potential of two viral isolates in herbaceous host Nicotiana benthamiana. Significantly higher accumulation of PPV capsid protein in tobacco leaves infected with PPV-CR (RU-30sc isolate) was detected in contrast to PPV-C (BY-101 isolate). This result correlated well with the symptoms observed in the infected plants. To further explore the host response upon viral infection at the molecular level, a comprehensive proteomic profiling was performed. Using reverse-phase UHPLC followed by label-free mass spectrometry quantification, we identified 38 unique plant proteins as significantly altered due to the infection. Interestingly, the abundances of photosynthesis-related proteins, mainly from the Calvin-Benson cycle, were found more aggressively affected in plants infected with PPV-CR isolate than those of PPV-C. This observation was accompanied with a significant reduction in the amount of photosynthetic pigments extracted from the leaves of PPV-CR infected plants. Shifts in the abundance of proteins that are involved in stimulation of photosynthetic capacity, modification of amino acid and carbohydrate metabolism may affect plant growth and initiate energy formation via gluconeogenesis in PPV infected N. benthamiana. We suggest that the higher accumulation of H2O2 in PPV-CR infected leaves plays a crucial role in plant defense and development by activating the glutathione synthesis.

    更新日期:2018-08-07
  • Development of in planta chemical cross-linking-based quantitative interactomics in Arabidopsis
    J. Proteome Res. (IF 3.95) Pub Date : 2018-08-07
    Shichang Liu, Fengchao Yu, Qin Hu, Tingliang Wang, Lujia Yu, Shengwang Du, Weichuan Yu, Ning Li

    An in planta chemical cross-linking-based quantitative interactomics (IPQCX-MS) workflow has been developed to investigate in vivo protein-protein interactions and alteration in protein structures in a model organism, Arabidopsis thaliana. A chemical cross-linker, azide-tag-modified disuccinimidyl pimelate (AMDSP), was directly applied onto Arabidopsis tissues. Peptides produced from protein fractions of CsCl density gradient centrifugation were dimethyl-labelled, from which the AMDSP cross-linked peptides were fractionated on chromatography, enriched, and analysed by mass spectrometry. ECL2 and SQUA-D software was used to identify and quantitate these cross-linked peptides, respectively. These computer programs integrate peptide identification with quantitation and statistical evaluation. This workflow eventually identified 354 unique cross-linked peptides, including 61 and 293 inter- and intra-protein cross-linked peptides, respectively, demonstrating that it is able to in vivo identify hundreds of cross-linked peptides at an organismal level by overcoming the difficulties caused by multiple cellular structures and complex secondary metabolites of plants. Co-immunoprecipitation and super-resolution microscopy studies have confirmed the PHB3- PHB6 protein interaction found by IPQCX-MS. The quantitative interactomics also found hormone-induced structural changes of SBPase and other proteins. This mass spectrometry-based interactomics will be useful in the study of in vivo protein-protein interaction networks in agricultural crops and plant-microbe interactions.

    更新日期:2018-08-07
  • Transcriptional and proteomic analysis revealed a synergistic effect of aflatoxin M1 and ochratoxin A mycotoxins on intestinal epithelial integrity of differentiated human Caco-2 cells
    J. Proteome Res. (IF 3.95) Pub Date : 2018-08-06
    Yanan Gao, Songli Li, Xiaoyu Bao, Chaochao Luo, Huaigu Yang, Jiaqi Wang, Shengguo Zhao, Nan Zheng

    Aflatoxin M1 (AFM1) is a common mycotoxin in dairy milk and it is typically concurrently present with other mycotoxins that may represent a threat for food safety. However, knowledge on how AFM1, alone or in combination with other mycotoxins may affect human intestinal epithelial integrity remain to be established. We employed transcriptome and proteome analysis integrated with biological validation to reveal the molecular basis underlining the effect AFM1 and/or ochratoxin A (OTA) exposure on intestinal epithelial integrity of differentiated Caco-2 cells. Exposure to 4 μg/ml of OTA was found to disrupt human gut epithelial integrity, whereas 4 μg/ml of AFM1 did not. Integrated transcriptome and proteome analysis of AFM1 and OTA, alone or in combination, indicate synergistic effect of the two mycotoxins in disrupting intestinal integrity. This effect was mechanistically linked to a broad ranges of pathways related to intestinal integrity enriched by down-regulated genes and proteins, associated to focal adhesion, adherens junction, and gap junction pathways. Furthermore, the cross–omics analysis of mixed AFM1 and OTA compared with OTA alone suggest that kinases family members, including MLCK, MAPKs, and PKC are the potential key regulators on modulating intestinal epithelial integrity. These findings provide novel insight into the synergistic detrimental role of multiple mycotoxins in disrupting intestinal integrity, and, therefore, identify potential target to improve milk safety related to human health. Transcriptomic raw data is available on Sequence Read Archive (SRA) database and the SRA accession is SRP133808 (https://www.ncbi.nlm.nih.gov/sra/SRP133808). The mass spectrometry proteomics data have been deposited to the ProteomeXchange Consortium (http://proteomecentral.proteomexchange.org) via the PRIDE partner repository with the dataset identifier PXD009437 and 10.6019/PXD009437.

    更新日期:2018-08-06
  • Proteomic Analysis of Charcoal-Stripped Fetal Bovine Serum Reveals Changes in the Insulin-like Growth Factor Signaling Pathway
    J. Proteome Res. (IF 3.95) Pub Date : 2018-08-03
    Chengjian Tu, Michael V. Fiandalo, Elena Pop, John J. Stocking, Gissou Azabdaftari, Jun Li, Hua Wei, Danjun Ma, Jun Qu, James L. Mohler, Li Tang, Yue Wu
    更新日期:2018-08-03
  • EXTENSIVE CHARACTERIZATION OF THE HUMAN SALIVARY BASIC PROLINE-RICH PROTEINS FAMILY BY TOP-DOWN MASS SPECTROMETRY
    J. Proteome Res. (IF 3.95) Pub Date : 2018-07-31
    Alessandra Padiglia, Roberto Orrù, Mozhgan Boroumand, Alessandra Olianas, Barbara Manconi, Maria Teresa Sanna, Claudia Desiderio, Federica Iavarone, Barbara Liori, Irene Messana, Massimo Castagnola, Tiziana Cabras

    Human basic proline-rich proteins and basic glycosylated proline-rich proteins, encoded by the polymorphic PRB1-4 genes and expressed only in parotid glands, are the most complex family of adult salivary proteins. The family includes 11 parent peptides/proteins and more than 6 parent glycosylated proteins, but a high number of proteoforms with rather similar structures derive from polymorphisms and post-translational modifications. In the present study 55 new components of the family were characterized by top-down liquid chromatography-mass spectrometry and tandem-mass platforms, bringing the total number of proteoforms to 110. The new components comprise the three variants P-H S1→A, P-Ko P36→S, and P-Ko A41→S and several their naturally occurring proteolytic fragments. The paper represents an updated reference for the peptides included in the heterogeneous family of proteins encoded by PRB1/PRB4. MS data are available via ProteomeXchange with the identifier PXD009813.

    更新日期:2018-08-01
  • 更新日期:2018-07-31
  • HyPR-MS for Multiplexed Discovery of MALAT1, NEAT1, and NORAD lncRNA Protein Interactomes
    J. Proteome Res. (IF 3.95) Pub Date : 2018-07-31
    Michele Spiniello, Rachel A. Knoener, Maisie I. Steinbrink, Bing Yang, Anthony J. Cesnik, Katherine E. Buxton, Mark Scalf, David F. Jarrard, Lloyd M. Smith
    更新日期:2018-07-31
  • Metabolic Response in Rabbit Urine to Occurrence and Relief of Unilateral Ureteral Obstruction
    J. Proteome Res. (IF 3.95) Pub Date : 2018-07-31
    Zhenzhao Wang, Rui Xu, Guiping Shen, Jianghua Feng
    更新日期:2018-07-31
  • Affimers as an Alternative to Antibodies in an Affinity LC–MS Assay for Quantification of the Soluble Receptor of Advanced Glycation End-Products (sRAGE) in Human Serum
    J. Proteome Res. (IF 3.95) Pub Date : 2018-07-26
    Frank Klont, Marrit Hadderingh, Péter Horvatovich, Nick H. T. ten Hacken, Rainer Bischoff
    更新日期:2018-07-26
  • 更新日期:2018-07-26
  • rawDiag: An R Package Supporting Rational LC–MS Method Optimization for Bottom-up Proteomics
    J. Proteome Res. (IF 3.95) Pub Date : 2018-07-24
    Christian Trachsel, Christian Panse, Tobias Kockmann, Witold E. Wolski, Jonas Grossmann, Ralph Schlapbach
    更新日期:2018-07-24
  • Three-Dimensional Cell Culture Conditions Affect the Proteome of Cancer-Associated Fibroblasts
    J. Proteome Res. (IF 3.95) Pub Date : 2018-07-23
    Regine C. Tölle, Cedric Gaggioli, Jörn Dengjel
    更新日期:2018-07-24
  • Enhanced Quantitative LC-MS/MS Analysis of N-linked Glycans Derived from Glycoproteins Using Sodium Deoxycholate Detergent
    J. Proteome Res. (IF 3.95) Pub Date : 2018-07-20
    Rui Zhu, Shiyue Zhou, Wenjing Peng, Yifan Huang, Parvin Mirzaei, Kaitlyn Donohoo, Yehia Mechref
    更新日期:2018-07-21
  • Synovial Fluid Metabolites Differentiate between Septic and Nonseptic Joint Pathologies
    J. Proteome Res. (IF 3.95) Pub Date : 2018-07-20
    James R. Anderson, Marie M. Phelan, Peter D. Clegg, Mandy J. Peffers, Luis M. Rubio-Martinez
    更新日期:2018-07-21
  • Porphyromonas gingivalis Gingipains Display Transpeptidation Activity
    J. Proteome Res. (IF 3.95) Pub Date : 2018-07-20
    Lianyi Zhang, Paul D. Veith, N. Laila Huq, Yu-Yen Chen, Christine A. Seers, Keith J. Cross, Dhana G. Gorasia, Eric C. Reynolds
    更新日期:2018-07-21
  • Genetic Variation in 9p21 and the Plasma Proteome
    J. Proteome Res. (IF 3.95) Pub Date : 2018-07-19
    Sara Mahdavi, David J. A. Jenkins, Christoph H. Borchers, Ahmed El-Sohemy
    更新日期:2018-07-20
  • Transformative Opportunities for Single-Cell Proteomics
    J. Proteome Res. (IF 3.95) Pub Date : 2018-07-19
    Harrison Specht, Nikolai Slavov
    更新日期:2018-07-20
  • Multifunctional Activity-Based Protein Profiling of the Developing Lung
    J. Proteome Res. (IF 3.95) Pub Date : 2018-07-18
    Ethan G. Stoddard, Regan F. Volk, James P. Carson, Cecilia M. Ljungberg, Taylor A. Murphree, Jordan N. Smith, Natalie C. Sadler, Anil K. Shukla, Charles Ansong, Aaron T. Wright
    更新日期:2018-07-19
  • 更新日期:2018-07-18
  • Comparative Metabolomics Elucidates Postprandial Metabolic Modifications in Plasma of Obese Individuals with Metabolic Syndrome
    J. Proteome Res. (IF 3.95) Pub Date : 2018-07-17
    Mengyang Xu, Fanyi Zhong, Richard S. Bruno, Kevin D. Ballard, Jing Zhang, Jiangjiang Zhu
    更新日期:2018-07-18
  • Metabolic Profiling of Chloroacetanilide Herbicides in Earthworm Coelomic Fluid Using 1H NMR and GC–MS
    J. Proteome Res. (IF 3.95) Pub Date : 2018-07-16
    Corey M. Griffith, Melissa A. Morgan, Meredith M. Dinges, Caroline Mathon, Cynthia K. Larive
    更新日期:2018-07-18
  • 更新日期:2018-07-18
  • HTRA1-Dependent Cell Cycle Proteomics
    J. Proteome Res. (IF 3.95) Pub Date : 2018-07-13
    Jasmin Schillinger, Katharina Severin, Farnusch Kaschani, Markus Kaiser, Michael Ehrmann
    更新日期:2018-07-14
  • 更新日期:2018-07-14
  • Human Metabolome Changes after a Single Dose of 3,4-Methylenedioxymethamphetamine (MDMA) with Special Focus on Steroid Metabolism and Inflammation Processes
    J. Proteome Res. (IF 3.95) Pub Date : 2018-07-13
    Martina I. Boxler, Gabriel L. Streun, Matthias E. Liechti, Yasmin Schmid, Thomas Kraemer, Andrea E. Steuer
    更新日期:2018-07-14
  • 更新日期:2018-07-12
  • Estimating the Distribution of Protein Post-Translational Modification States by Mass Spectrometry
    J. Proteome Res. (IF 3.95) Pub Date : 2018-07-10
    Philip D. Compton, Neil L. Kelleher, Jeremy Gunawardena
    更新日期:2018-07-12
  • 更新日期:2018-07-12
  • 更新日期:2018-07-10
Some contents have been Reproduced with permission of the American Chemical Society.
Some contents have been Reproduced by permission of The Royal Society of Chemistry.
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