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  • A Self-Assembled Nanoparticles Platform Based on Pectin-Dihydroartemisinin Conjugates for Co-delivery of Anticancer Drugs
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-20
    Yanxue Liu, Dan Zheng, Yunyun Ma, Juan Dai, Chunxiao Li, Shangzhen Xiao, Kefeng Liu, Jing Liu, Luying Wang, Jiandu Lei, Jing He

    Natural pectin is an important carrier for delivering drugs in biomedical research, however, there are only a few reports on the preparation of pectin nanoparticles, especially a particle size of below 100 nm with high yield. Here we design pectin-dihydroartemisinin / hydrooxycampothecin nanoparticles (PDC-H NPs) through a self-assembly method. The prepared PDC-H NPs contained hydrophilic part of pectin and hydrophobic anticancer drugs of dihydroartemisinin and hydroxycamptothecin, which could increase drug loading, improve water-solubility, and achieve controlled release of drugs. The results indicated that the particle size of PDC-H NPs was about 70 nm, drug-loaded efficiency of DHA was 20.33 wt% and encapsulation efficiency of HCPT was 14.11 wt%. PDC-H NPs exhibited a higher cytotoxicity, the blood retention time of PDC-H NPs was 4.8-fold longer than DHA and was 6.8-fold longer than HCPT. In addition, effective cellular uptake exhibited an obvious synergistic effect compared with DHA and HCPT. 4T1 tumor-bearing mice also showed a higher survival rate than free DHA and free HCPT. The result show that the self-assembled PDC-H NPs is a promising anticancer drug for Co-delivery.

    更新日期:2017-11-21
  • 更新日期:2017-11-19
  • Using Biomimetic Polymers in Place of Noncollagenous Proteins to Achieve Functional Remineralization of Dentin Tissues
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-17
    Yung-Ching Chien, Jinhui Tao, Kuniko Saeki, Alexander F. Chin, Jolene L. Lau, Chun-Long Chen, Ronald N. Zuckermann, Sally J. Marshall, Grayson W. Marshall, James J. De Yoreo
    更新日期:2017-11-19
  • Absorbable Hemostatic Aggregates
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-17
    Allen Y. Wang, Joseph Rafalko, Melinda MacDonald, Xintian Ming, Richard Kocharian
    更新日期:2017-11-19
  • Promotion of Hernia Repair with High-Strength, Flexible, and Bioresorbable Silk Fibroin Mesh in a Large Abdominal Hernia Model
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-17
    Wei Zhang, Yu Li, Deming Jiang, Shujun Xie, Mengfeng Zeng, Jialin Chen, Longkun Chen, Hongwei Ouyang, Xiaohui Zou
    更新日期:2017-11-19
  • Biomaterial Property Effects on Platelets and Macrophages: An in Vitro Study
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-17
    Kelly R. Fernandes, Yang Zhang, Angela M. P. Magri, Ana C. M. Renno, Jeroen J. J. P. van den Beucken
    更新日期:2017-11-19
  • Evaluation of hydrogels presenting extracellular matrix-derived adhesion peptides and encapsulating cardiac progenitor cells for cardiac repair
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-17
    Srishti Bhutani, Aline Nachlas, Milton E Brown, Tionne Pete, Christopher T. Johnson, Andres Jose Garcia, Michael E Davis

    Cell therapy is an emerging paradigm for the treatment of heart disease. In spite of the exciting and promising preclinical results, the benefits of cell therapy for cardiac repair in patients have been modest at best. Biomaterials-based approaches may overcome the barriers of poor differentiation and retention of transplanted cells. In this study, we prepared and tested hydrogels presenting extracellular matrix (ECM)-derived adhesion peptides as delivery vehicles for c-kit+ cardiac progenitor cells (CPCs). We assessed their effects on cell behavior in vitro as well as cardiac repair in rats undergoing ischemia reperfusion. Hydrogels presenting the collagen-derived GFOGER peptide induced cardiomyocyte differentiation of CPCs as demonstrated by increased expression of cardiomyocyte structural proteins. However, conditioned media obtained from GFOGER hydrogels showed lower levels of secreted reparative factors. Interestingly, following injection in rats undergoing ischemia-reperfusion, treatment with CPCs encapsulated in non-adhesive RDG-presenting hydrogels resulted in the preservation of cardiac contractility and attenuation of post-infarct remodeling whereas the adhesion peptide-presenting hydrogels did not induce any functional improvement. Retention of cells was significantly higher when delivered with non-adhesive hydrogels compared to ECM-derived peptide gels. These data suggest that factors including cell differentiation state, paracrine factors and interaction with biomaterials influence the effectiveness of biomaterials-based cell therapy. A holistic consideration of these multiple variables should be included in cell-biomaterial combination therapy designs.

    更新日期:2017-11-19
  • Biomimetic Hyaluronic Acid-Lysozyme Composite Coating on AZ31 Mg Alloy with Combined Antibacterial and Osteoinductive Activities
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-16
    Sankalp Agarwal, Mathieu Riffault, David Hoey, Brendan Duffy, James Curtin, Swarna Jaiswal
    更新日期:2017-11-17
  • Functionalized Graphene Tagged Polyurethanes for Corrosion Inhibitor and Sustained Drug Delivery
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-16
    Dinesh K. Patel, Sudipta Senapati, Punita Mourya, Madan M. Singh, Vinod K. Aswal, Biswajit Ray, Pralay Maiti
    更新日期:2017-11-16
  • Self-Assembling Peptide Nanofiber Hydrogels for Controlled Ocular Delivery of Timolol Maleate
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-16
    Christina Karavasili, Anastasia Komnenou, Orestis L. Katsamenis, Glykeria Charalampidou, Evangelia Kofidou, Dimitrios Andreadis, Sotirios Koutsopoulos, Dimitrios G. Fatouros
    更新日期:2017-11-16
  • Injectable, Self-Healing Chimeric Catechol-Fe(III) Hydrogel for Localized Combination Cancer Therapy
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-16
    Prabhu S. Yavvari, Sanjay Pal, Sandeep Kumar, Animesh Kar, Anand Kumar Awasthi, Aaliya Naaz, Aasheesh Srivastava, Avinash Bajaj
    更新日期:2017-11-16
  • Dispersible MoS2 Nanosheets Activated TGF-β/Smad Pathway and Perturbed the Metabolome of Human Dermal Fibroblasts
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-16
    Yadong Yu, Na Wu, Yanliang Yi, Yangying Li, Lei Zhang, Qi Yang, Wenjun Miao, Xuefang Ding, Ling Jiang, He Huang

    In the post-graphene two-dimensional materials(2DMs), MoS2 attracts increasing attentions in biomedical field due to its excellent physicochemical properties. However, the toxicity or biocompatibility evaluation of MoS2 is not fully addressed. Herein, chitosan functionalized MoS2 (CS-MoS2) nanosheets, which showed perfect dispersibility and stability performances, were synthesized and characterized. We found that CS-MoS2 nanosheets inhibited the viability of human dermal fibroblasts (HDFs) moderately, while caused cell membrane instability, ROS generation and DNA damage in a dosage-dependent manner. CS-MoS2 nanosheets did not induce significant changes in the cell morphologies, but they seemed to impair the cell division of HDFs. 100 μg/mL of CS-MoS2 nanosheets activated EGFR and induced ROS, Smad and IL-1, which in turn led to cell inflammation and apoptosis. Furthermore, HDFs showed cellular stress responses when they were exposed to low concentrations of CS-MoS2 nanosheets (25, 100 μg/mL), since most of the intracellular metabolites such as amino acids were induced by the concentration of 25 μg/mL, but inhibited by 100 μg/mL. Pyroglutamic acid, phosphoric acid, and inositol might be used as the biomarkers for evaluating the toxicity of CS-MoS2 nanosheets. Additionally, 100 μg/mL CS-MoS2 nanosheets inhibited glutathione metabolism and induced the imbalance of cellular redox homeostasis. It further suppressed TCA cycle and other metabolic pathways, causing insufficient supply of substrates and energy for HDFs. These findings will fuel the risk assessment of MoS2 and other 2DMs, and guide the safe material design and 2DMs applications.

    更新日期:2017-11-16
  • Fabrication scheme for obtaining transparent, flexible, and water-insoluble silk films from apparently dissolved silk-gland fibroin of Bombyx mori silkworm
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-16
    Taiyo YOSHIOKA, Tamako Hata, Katsura Kojima, Yasumoto Nakazawa, Tsunenori Kameda

    Films from silk fibroin-protein are one of the most promising biomaterials because of their exquisite balance between the mechanical properties and biocompatibility. Numerous schemes have been proposed for processing fibroin film, utilizing liquid silk fibroin (LF) or regenerated silk fibroin (RF). The films casted from LF or RF in the solution state are water soluble, and therefore require additional post-production treatment with alcohols or other polar solvents to induce β-sheet formation, to render them insoluble in water. However, the post-production treatment renders the fibroin films less-flexible or even highly brittle. In order to overcome this issue, we developed a novel scheme for fibroin processing using silk-gland fibroin (SGF). The objective of this scheme is to create a softly solidified fibroin-gel-state of the silk glands with an imperfect β-sheet structure, by treating them with an ethanol/water mixture. Such fibroin gel was found to dissolve in 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP). The SGF film cast from the HFIP solution is water-insoluble without brittleness. In addition to this improvement, the SGF film produced by this method contains a significantly low level of residual HFIP molecules compared to the traditional RF films prepared from an HFIP solution. The mechanism underlying these advantageous characteristics was investigated from the structural viewpoint, by using techniques such as 13C solid-state NMR, differential scanning calorimetry, and wide-angle X-ray diffraction.

    更新日期:2017-11-16
  • 更新日期:2017-11-16
  • Contribution of the Polarity of Mussel-Inspired Adhesives in the Realization of Strong Underwater Bonding
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-15
    Youbing Mu, Xiao Wu, Danfeng Pei, Zelin Wu, Chen Zhang, Dongshan Zhou, Xiaobo Wan
    更新日期:2017-11-16
  • 更新日期:2017-11-16
  • Pro-angiogenic properties of terbium hydroxide nanorods: Molecular mechanisms and therapeutic applications in wound healing
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-15
    Susheel Kumar Nethi, Ayan Kumar Kumar Barui, vishnusravan bollu, Bonda Rama Rao, Chitta Ranjan Patra

    The process of angiogenesis, involving generation of new blood vessels from the existing ones, is vital for the supply of oxygen and nutrients to various tissues of body system. Angiogenesis is directly associated with several physiological and pathological processes. It is well established that impairment in angiogenesis process results in various fatal conditions. Recently, few research groups including ours demonstrated therapeutic angiogenesis through nanomedicine approach using metal oxide/hydroxide nanoparticles. However, there is still a thorough necessity for the development of novel, eco-friendly, pro-angiogenic nanomaterials. Hence, in the present study we demonstrate the in vitro and in vivo pro-angiogenic properties of terbium hydroxide nanorods (THNRs) synthesized using an advanced microwave irradiation method, along with the detailed molecular signaling cascade underlying THNRs induced angiogenesis. The in vivo wound healing and non-immunogenicity of the THNRs have been validated in the mouse models. We thus strongly believe that the present study establishing the pro-angiogenic properties of THNRs will aid in the development of alternative treatment strategies for wound healing along with cardiovascular and ischemic diseases, where angiogenesis is the chief target.

    更新日期:2017-11-16
  • Rationally designed calcium phosphate/small gold nanorod assemblies using poly(acrylic acid calcium salt) nanospheres as templates for chemo-photothermal combined cancer therapy
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-14
    Shengnan Li, Lingyu Zhang, Haipeng Zhang, Zhongcheng Mu, Lu Li, Chungang Wang

    Elaborately designed novel multifunctional therapeutic agents are highly desired for efficient cancer therapy. In this work, a new therapeutic nanoplatform based on calcium phosphate/small gold nanorod assemblies modified with methoxy-poly(ethylene glycol)-thiol (designated as PEGylated CaP/Au NR assemblies) is created via a mild, reproducible and simple route for the first time. The obtained PEGylated CaP/Au NR assemblies possess a lot of virtues including outstanding drug loading capacity, excellent photothermal conversion efficiency (η, ~38.5%), pH/near infrared (NIR) dual-responsive release property and good biocompatibility. After loading doxorubicin (DOX) in PEGylated CaP/Au NR assemblies, the DOX-loaded PEGylated CaP/Au NR assemblies can simultaneously supply intense heating effect and increased DOX release under 808 nm NIR laser, achieving excellent antitumor therapeutic effect in vitro and in vivo. Furthermore, the combination of DOX-loading and photothermal treatment upon PEGylated CaP/Au NR assemblies displays better therapeutic effect than single chemotherapy or photothermal therapy. Furthermore, the comprehensive methyl thiazolyl tetrazolium (MTT), hemolysis and histological assays manifest no obvious toxicity of PEGylated CaP/Au NR assemblies. Our work elucidates the great prospect of PEGylated CaP/Au NR assemblies as a therapeutic agent for synergistic chemo-photothermal cancer therapy.

    更新日期:2017-11-15
  • An Electrospun Decellularized Lung Matrix Scaffold for Airway Smooth Muscle Culture
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-13
    Bethany M. Young, Keerthana Shankar, Brittany P. Allen, Robert A. Pouliot, Matthew B. Schneck, Nabil Mikhaiel, Rebecca L. Heise

    Chronic respiratory disease affects many people worldwide with little known about the intricate mechanisms driving the pathology, making it difficult to develop novel therapies. Improving the understanding of airway smooth muscle and extracellular matrix (ECM) interactions is key to developing treatments for this leading cause of death. With currently no relevant or controllable in vivo or in vitro models to investigate cell-ECM interactions in the small airways, the development of a biomimetic in vitro model with cell attachment, signaling, and organization is needed. The goal of this study was to create a biologically and structurally relevant in vitro model of small airway smooth muscle. In order to achieve this goal, a scaffold was engineered from synthetic poly-L-lactic acid (PLLA) and decellularized pig lung ECM (PLECM). PLECM scaffolds have improved physical characteristics over synthetic scaffolds, by exhibiting a significant decrease in the elastic modulus and an increase in hydrophilicity. Histological staining and SDS-PAGE showed that essential proteins or protein fragments found in natural ECM were present after processing. Human bronchial smooth muscle cells (HBSMCs) seeded onto PLECM 3D scaffolds formed confluent layers and maintained a contractile phenotype, as demonstrated by the organized arrangement of actin filaments within the cell and expected contractile protein expression of calponin 1. HBSMCs cultured on electrospun PLECM scaffold also increased alpha-1 type 1 collagen compared to those cultured on PLLA scaffolds. In summary, this research demonstrates that a PLLA/PLECM composite electrospun mat is a promising tool to produce an in vitro model of the airway with the potential for a better understanding of bronchiole smooth muscle behavior in diseased or normal states.

    更新日期:2017-11-14
  • Designing biodegradable PHA-based 3D scaffolds with antibiofilm properties for wound dressings: optimization of the micro/nanostructure
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-13
    Aracelys Maria Marcano, Naila Bou Haidar, Stéphane Marais, Jean Marc Valleton, Anthony C Duncan

    One major factor inhibiting natural wound-healing processes is infection through bacterial biofilms, particularly in the case of chronic wounds. In this study, the micro/nanostructure of a wound dressing was optimized in order to obtain a more efficient antibiofilm protein-release profile for biofilm inhibition and/or detachment. A 3D substrate was developed with asymmetric polyhydroxyalkanoate (PHA) membranes to entrap Dispersin B (DB), the antibiofilm protein. The membranes were prepared using wet induced phase separation (WIPS). By modulating the concentration and the molecular weight of the porogen polymer, polyvinylpyrrolidone (PVP), asymmetric membranes with controlled porosity were obtained. PVP was added at 10, 30 and 50% w/w, relative to the total polymer concentration. The physical and kinetic properties of the quaternary non-solvent/solvent/PHA/PVP systems were studied and correlated with the membrane structures obtained. The results show that at high molecular weight (Mw= 360 KDa) and high PVP content (above 30%), pore size decreased and the membrane became extremely brittle with serious loss of physical integrity. This brittle effect was not observed for low molecular weight PVP (Mw= 40 KDa) at comparable contents. Whatever the molecular weight, porogen content up to 30% increased membrane surface porosity and consequently protein uptake. Above 30% porogen content, the pore size and the physical integrity/mechanical robustness both decreased. The PHA membranes were loaded with DB and their antibiofilm activity was evaluated against Staphylococcus epidermidis biofilms. When the bacterial biofilms were exposed to the DB-loaded PHA membrane, up to 33 % of the S. epidermidis biofilm formation was inhibited, while 26% of the biofilm already formed was destroyed. These promising results validate our approach based on the development of bioactive-protein-loaded asymmetric membranes for antibiofilm strategies in situations where traditional antibiotic therapies are ineffective.

    更新日期:2017-11-14
  • The comparison of NMDA and AMPA channel expression and function between embryonic and adult neurons utilizing microelectrode array systems
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-13
    Darin Edwards, Frank Sommerhage, Bonnie Berry, Hanna Nummer, Martina Raquet, Brad Clymer, Maria Stancescu, James J. Hickman

    Microelectrode arrays (MEAs) are innovative tools used to perform electrophysiological experiments for the study of electrical activity and connectivity in populations of neurons from dissociated cultures. The reliance upon neurons derived from embryonic tissue is a common limitation of neuronal/microelectrode array (MEA) hybrid systems, and perhaps of neuroscience research in general, and the use of adult neurons could model fully functional in vivo parameters more closely. Spontaneous network activity was concurrently recorded from both embryonic and adult rat neurons cultured on MEAs for up to 10 weeks in vitro to characterize the synaptic connections between cell types. The cultures were exposed to synaptic transmission antagonists against NMDA and AMPA channels which revealed significantly different receptor profiles of adult and embryonic networks in vitro. In addition, both embryonic and adult neurons were evaluated for NMDA and AMPA channel subunit expression over 5 weeks in vitro. The results established that neurons derived from embryonic tissue did not express mature synaptic channels for several weeks in vitro under defined conditions. Consequently, the embryonic response to synaptic antagonists was significantly different than that of neurons derived from adult tissue sources. These results are especially significant because most studies reported with embryonic hippocampal neurons do not begin at 2 to 4 weeks in culture. In addition, the utilization of MEAs in lieu of patch-clamp electrophysiology avoided a large-scale, labor-intensive study. These results establish the utility of this unique hybrid system derived from adult hippocampal tissue in combination with MEAs and offers a more appropriate representation of in vivo function for drug discovery. It has application for neuronal development and regeneration as well as for investigations into neurodegenerative disease, traumatic brain injury, and stroke.

    更新日期:2017-11-14
  • On-chip fabrication of a cell-derived extracellular matrix sheet
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-13
    Yoonmi Hong, Ilkyoo Koh, Kwideok Park, Pilnam Kim

    The extracellular matrix (ECM) provides physical and chemical support to the surrounding cells. During cell growth, ECM secretion and network formation influence on cell morphology, cell adhesion, cell-to-cell interactions and cell migration. Microfluidics-based cell culture systems are limited by the integration of structural ECM into the device. We report the development of a cell-derived ECM-incorporated microfluidic device that can provide structural characteristics and biochemical components of cell-derived ECM. Using an on-chip decellularization process, we constructed an ECM sheet, secreted and deposited from monolayer-cultured mouse embryonic fibroblasts (NIH/3T3), inside the microfluidic device. ECM components (including collagens, fibronectin, laminin, and elastin) and mesh-type fibronectin fibrous architecture were maintained on the surface of the porous membrane of the microfluidic device after decellularization. To verify the usability of the fibroblast-derived ECM sheet integrated microfluidic device in a cell culture platform, we tested the recelluralization of human umbilical vein endothelial cells (HUVEC), and analyzed HUVEC–ECM and HUVEC–HUVEC interactions. On the ECM sheet, HUVECs exhibited morphologies and focal adhesion features that were markedly different from those of other groups. We then explored the effect of the ECM sheet on HUVEC mechanosensitivity. An increase in fluid shear stresses led to focal adhesion and the polymerization and reorganization of HUVEC adherens junctions, similar to natural junctional development, whereas the control group exhibited stimuli-insensitive behaviors. We conclude that the decellularized ECM sheet-incorporated microfluidic device provides an in vivo-like physical and biochemical ECM microenvironment for microfluidics-based cell culture.

    更新日期:2017-11-14
  • Decellularized matrices as cell-instructive scaffolds to guide tissue-specific regeneration
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-13
    Kevin P Robb, Arthi Shridhar, Lauren Flynn

    Decellularized scaffolds are promising clinically-translational biomaterials that can be applied to direct cell responses and promote tissue regeneration. Bioscaffolds derived from the extracellular matrix (ECM) of decellularized tissues can naturally mimic the complex extracellular microenvironment through the retention of compositional, biomechanical, and structural properties specific to the native ECM. Increasingly, studies have investigated the use of ECM-derived scaffolds as instructive substrates to recapitulate properties of the stem cell niche and guide cell proliferation, paracrine factor production, and differentiation in a tissue-specific manner. Here, we review the application of decellularized tissue scaffolds as instructive matrices for stem or progenitor cells, with a focus on the mechanisms through which ECM-derived scaffolds can mediate cell behaviour to promote tissue-specific regeneration. We conclude that while additional pre-clinical studies are required, ECM-derived scaffolds are a promising platform to guide cell behaviour and may have widespread clinical applications in the field of regenerative medicine.

    更新日期:2017-11-14
  • Sodium Alginate Toughening of Gelatin Hydrogels
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-13
    Michael Anthony Samp, Nicolae C Iovanac, Adam John Nolte

    Gelatin is a popular material for the creation of tissue phantoms due to its ease-of-use, safety, low relative cost, and its amenability to tuning physical properties through the use of additives. One difficulty that arises when using gelatin, especially in low concentrations, is the brittleness of the material. In this paper, we show that small additions of another common biological polymer, sodium alginate, significantly increases the toughness of gelatin without changing the Young’s modulus or other low-strain stress relaxation properties of the material. Samples were characterized using ramp-hold stress relaxation tests. The experimental data from these tests was then fit to the Generalized Maxwell (GM) model, as well as two models based on a fractional calculus approach: the Kelvin-Voigt Fractional Derivative (KVFD) and Fractional Maxwell (FM) models. We found that for our samples, the fractional models provided better fits with fewer parameters, and at strains within the linear elastic region, the linear viscoelastic parameters of the alginate/gelatin and pure gelatin samples were essentially indistinguishable. When the same ramp-hold stress relaxation experiments were run at high strains outside of the linear elastic region, we observed a shift in stress relaxation to shorter time scales with increasing sodium alginate addition, which may be associated with an increase in fluidity within the gelatin matrix. This leads us to believe that increasing amounts of sodium alginate acts to enhance the viscosity within the fluidic region of the gelatin matrix, providing additional energy dissipation without raising the modulus of the material. These results are applicable to anyone desiring independent control of the Young’s modulus and toughness in preparing tissue phantoms, and suggest that sodium alginate should be added to low-modulus gelatin for use in biological and medical testing applications.

    更新日期:2017-11-13
  • R and Z-axis Patterned Scaffolds Mimic Tracheal Circumferential Compliance and Longitudinal Extensibility
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-13
    Elizabeth Mansfield Boazak, Jamie Benson, Debra T. Auguste

    There remains no routine treatment for congenital tracheal abnormalities affecting more than 1/3rd of the length. Natural and artificial prostheses are plagued by mechanical failure and inconsistent outcomes. Mimicking native tissue mechanics in an engineered replacement may improve functional and patient outcomes. We synthesized tubular constructs comprised of photocrosslinked methyl acrylate – co – methyl methacrylate, p(MA-co-MMA), with patterned r and z-axes in order to achieve mechanical properties similar to lamb tracheae. Hard and soft alternating bands, and a soft vertical section, mimic tracheal architecture. Patterned constructs were capable of 46% elastic longitudinal extension. The construct longitudinal composite modulus, 0.34 ± 0.09 MPa, was not significantly different from ovine tracheae. The superior of two geometries evaluated supports up to a 46% reduction of internal volume within the physiological range of transmural pressures. Thus, these patterned hydrogels yielded longitudinal elasticity and radial rigidity while allowing for radial deformation required for effective coughing.

    更新日期:2017-11-13
  • Liver-kidney-on-chip to study toxicity of drug metabolites
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-12
    Jannick Theobald, Ali Ghanem, Patrick Wallisch, Amin A. Banaeiyan, Miguel A. Andrade-Navarro, Katarina Taskova, Manuela Haltmeier, Andreas Kurtz, Holger Becker, Stefanie Reuter, Ralf Mrowka, Xinlai Cheng, Stefan Wölfl

    Advances in organ-on-chip technologies for the application in in-vitro drug development provide an attractive alternative approach to replace ethically controversial animal testing and to establish a basis for accelerated drug development. In recent years, various chip-based tissue culture systems have been developed, which are mostly optimized for cultivation of one single cell type or organoid structure and lack the representation of multi organ interactions. Here we present an optimized microfluidic chip design consisting of interconnected compartments, which provides the possibility to mimic the exchange between different organ specific cell types and enables to study interdependent cellular responses between organs and demonstrate that such tandem system can greatly improve the reproducibility and efficiency of toxicity studies. In a simplified liver-kidney-on-chip model, we showed that hepatic cells which grow in microfluidic conditions abundantly and stably expressed metabolism-related biomarkers. Moreover, we applied this system for investigating the biotransformation and toxicity of Aflatoxin B1 (AFB1) and Benzoalphapyrene (BαP), as well as the interaction with other chemicals. The results clearly demonstrate that the toxicity and metabolic response to drugs can be evaluated in a flow-dependent manner within our system, supporting the importance of advanced interconnected multi-organs in microfluidic devices for application in in vitro toxicity testing and as optimized tissue culture systems for in vitro drug screening.

    更新日期:2017-11-13
  • Polymer Microneedle Mediated Local Aptamer Delivery for Blocking the Function of Vascular Endothelial Growth Factor
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-10
    James Coyne, Brandon Davis, David Kauffman, Nan Zhao, Yong Wang
    更新日期:2017-11-11
  • A Decellularized Matrix Produced by Mesenchymal Stem Cells Modulates Growth and Metabolic Activity of Hepatic Cell Cluster
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-10
    Jooyeon Park, Joyeon Kim, Kathryn Michele Sullivan, Seungyun Baik, Eunkyung Ko, Myung-Joo Kim, Young Jun Kim, Hyunjoon Kong

    Miniature organ-like three-dimensional cell clusters often called organoids have emerged as a useful tool for both fundamental and applied bioscience studies. However, there is still a great need to improve the quality of organoids to a level where they exhibit similar biological functionality to an organ. To this end, we hypothesized that a decellularized matrix derived from mesenchymal stem cell (MSC) could regulate the phenotypic and metabolic activity of organoids. This hypothesis was examined by culturing cells of interest in the decellularized matrix of MSCs cultured on a 2D substrate at confluency or in the form of spheroids. The decellularized matrix prepared with MSC spheroids showed a 3D porous structure with a higher content of extracellular matrix molecules than the decellularized matrix derived from MSCs cultured on a 2D substrate. HepG2 hepatocarcinoma cells, which retain the metabolic activity of hepatocytes, were cultured in these decellularized matrices. Interestingly, the decellularized matrix from the MSC spheroids served to develop the hepatic cell clusters with higher levels of E-cadherin-mediated cell-cell adhesion and detoxification activity than the decellularized matrix from the MSCs cultured on a 2D substrate. Overall, the results of this study are useful in improving biological functionality of a wide array of organoids.

    更新日期:2017-11-11
  • The promotion of hernia repair with high-strength, flexible and bioresorbable silk fibroin mesh in a large abdominal hernia model
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-09
    Wei Zhang, Yu Li, Deming Jiang, Shujun Xie, Mengfeng Zeng, Jialin Chen, Longkun Chen, HongWei Ouyang, Xiaohui Zou

    The use of synthetic surgical meshes for abdominal hernia repair presents numerous challenges due to insufficient mechanical strength, non-absorbability and implant rigidity that leads to complications including chronic inflammatory reactions and adhesions. In this study, a naturally-derived, high-strength, flexible and bioresorbable silk fibroin mesh was developed by knitted textile engineering and biochemical manipulation. The mechanical properties of the mesh were optimized with the trial of different surface coating methods (thermal or chemical treatment) and 12 different knit patterns. The developed silk fibroin mesh showed sufficient tensile strength (67.83 N longitudinally and 62.44 N vertically) which afforded the high mechanical strength required for abdominal hernia repair (16 N). Compared to the commonly-used commercial non-absorbable and absorbable synthetic meshes (Prolene® and Ultrapro® respectively), the silk fibroin mesh showed advantages over synthetic meshes, including lower elongation rate (47.14% longitudinally and 67.15% vertically), lower stiffness (10 to 1000 fold lower, p<0.001), as well as lower anisotropic behavior. In a rat model of large abdominal hernia repair, our mesh facilitated effective hernia repair with minimal chronic inflammation which gradually decreased from 15 to 60 days postoperatively, as well as lower adhesion formation rate and scores compared to the control meshes. More abundant and organized collagen deposition, together with more pronounced neo-vascularization were observed in the repaired tissue treated with silk fibroin mesh as compared to that treated with synthetic meshes. Besides, the silk fibroin mesh gradually transferred load-bearing responsibilities to the repaired host tissue as it was bioresorbed after implantation. Its isotropic architecture favored an ease of use during operations. In summary, our findings indicate that the use of knitted silk fibroin mesh provides a safe and effective alternative solution for large abdominal hernia repairs as it overcomes the prevailing limitations associated with synthetic meshes.

    更新日期:2017-11-09
  • Advances in Multiscale Characterization Techniques of Bone and Biomaterials Interfaces
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-09
    Dakota M. Binkley, Kathryn Grandfield
    更新日期:2017-11-09
  • Long-Term Preservation of Bacteriophage Antimicrobials Using Sugar Glasses
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-09
    Vincent Leung, Alexandra Szewczyk, Jacqueline Chau, Zeinab Hosseinidoust, Logan Groves, Hajar Hawsawi, Hany Anany, Mansel W. Griffiths, M. Monsur Ali, Carlos D. M. Filipe
    更新日期:2017-11-09
  • 更新日期:2017-11-09
  • Targeted Hyaluronate–Hollow Gold Nanosphere Conjugate for Anti-Obesity Photothermal Lipolysis
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-09
    Jung Ho Lee, Hyeon Seon Jeong, Dong Hyun Lee, Songeun Beack, Taeyeon Kim, Geon-Hui Lee, Won Chan Park, Chulhong Kim, Ki Su Kim, Sei Kwang Hahn
    更新日期:2017-11-09
  • Computational Framework to Predict Failure and Performance of Bone-Inspired Materials
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-08
    Flavia Libonati, Vito Cipriano, Laura Vergani, Markus J. Buehler
    更新日期:2017-11-09
  • Hollow Copper Sulfide Nanosphere–Doxorubicin/Graphene Oxide Core–Shell Nanocomposite for Photothermo-chemotherapy
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-08
    Lu Han, Ya-Nan Hao, Xing Wei, Xu-Wei Chen, Yang Shu, Jian-Hua Wang
    更新日期:2017-11-09
  • In Situ Printing-then-Mixing for Biological Structure Fabrication Using Intersecting Jets
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-08
    Kyle Christensen, Ashley Compaan, Wenxuan Chai, Guangbin Xia, Yong Huang
    更新日期:2017-11-09
  • Biomaterial property effects on platelets and macrophages: an in vitro study
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-07
    Kelly Fernandes, Yang Zhang, Angela Magri, Ana Rennó, Jeroen JJP van den Beucken

    The purpose of this study was to evaluate the effect of surface properties of bone implants coated with hydroxyapatite (HA) and β-tricalcium phosphate (β-TCP) on platelets and macrophages upon implant installation and compare to grit-blasted Ti and Thermanox used as a control. Surface properties were characterized using scanning electron microscopy, profilometry, crystallography, FTIR and coating stability. For platelets, platelet adherence and morphology were assessed. For macrophages, morphology, proliferation, and polarization were evaluated. Surface characterization showed similar roughness of ~2.5 µm for grit-blasted Ti discs, both with and without coating. Coating stability assessment showed substantial dissolution of HA and β-TCP coatings. Platelet adherence was significantly higher for grit-blasted Ti,Ti-HA and and Ti-β-TCP coatings compared to cell culture control Thermanox. Macrophage cultures revealed a decreased proliferation on both HA and β-TCP coated discs compared to both Thermanox and grit-blasted Ti. In contrast, secretion of pro-inflammatory cytokine TNF-α and anti-inflammatory cytokine TGF-β were marginal for grit-blasted Ti and Thermanox while a coating-dependent increased secretion of pro- and anti- inflammatory cytokines was observed for HA and β-TCP coatings. The results demonstrated a significantly upregulated pro-inflammatory and anti-inflammatory cytokine secretion and marker gene expression of macrophages on HA and β-TCP coatings. Furthermore, HA induced an earlier M1 macrophage polarization but more M2 phenotype potency than β-TCP. In conclusion, our data showed that material surface affects the behaviors of first cell types attached to implants. Due to the demonstrated crucial roles of platelets and macrophages in bone healing and implant integration, these information would great aid the design of metallic implants for a higher rate of success in patients.

    更新日期:2017-11-08
  • Methods to assess shear-thinning hydrogels for application as injectable biomaterials
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-07
    Minna Chen, Leo Wang, Jennifer Chung, Young-Hun Kim, Pavan Atluri, Jason A. Burdick

    Injectable hydrogels have gained popularity as a vehicle for the delivery of cells, growth factors, and other molecules to localize and improve their retention at the injection site, as well as for the mechanical bulking of tissues. However, there are many factors, such as viscosity, storage and loss moduli, and injection force, to consider when evaluating hydrogels for such applications. There are now numerous tools that can be used to quantitatively assess these factors, including for shear-thinning hydrogels since their properties change under mechanical load. Here, we describe relevant rheological tests and ways to measure injection force using a force sensor or a mechanical testing machine toward the evaluation of injectable hydrogels. Injectable, shear-thinning hydrogels can be used in a variety of clinical applications, and as an example we focus on methods for injection into the heart, where an understanding of injection properties and mechanical forces is imperative to consistent hydrogel delivery and retention. We discuss methods for delivery of hydrogels to mouse, rat, and pig hearts in models of myocardial infarction, and compare methods of tissue post-processing for hydrogel preservation. Our intent is that the methods described herein can be helpful in the design and assessment of shear-thinning hydrogels for widespread biomedical applications.

    更新日期:2017-11-08
  • Near-Infrared Guided Thermal-Responsive Nanomedicine against Orthotopic Superficial Bladder Cancer
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-07
    Hui Gao, Ying Bi, Xin Wang, Miao Wang, Mengxue Zhou, Huiru Lu, Jimin Gao, Jun Chen, Yi Hu
    更新日期:2017-11-08
  • Europium Doped Calcium Deficient Hydroxyapatite as Theranostic Nanoplatforms: Effect of Structure and Aspect Ratio
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-07
    Sunita Prem Victor, M.G. Gayathri Devi, Willi Paul, Vineeth M. Vijayan, Jayabalan Muthu, Chandra P. Sharma
    更新日期:2017-11-08
  • Facile Synthesis of Sulfobetaine-Stabilized Cu2O Nanoparticles and Their Biomedical Potential
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-07
    Marta J. Woźniak-Budych, Łucja Przysiecka, Barbara M. Maciejewska, Daria Wieczorek, Katarzyna Staszak, Marcin Jarek, Teofil Jesionowski, Stefan Jurga
    更新日期:2017-11-08
  • Nitrilotriacetic Acid (NTA) and Phenylboronic Acid (PBA) Functionalized Nanogels for Efficient Encapsulation and Controlled Release of Insulin
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-07
    Chang Li, Gang Wu, Rujiang Ma, Yong Liu, Ying Liu, Juan Lv, Yingli An, Linqi Shi
    更新日期:2017-11-08
  • Turning Expanded Poly(tetrafluoroethylene) Membranes into Potential Skin Wound Dressings by Grafting a Bioinert Epoxylated PEGMA Copolymer
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-07
    Antoine Venault, Cheng-Sian Liou, Lu-Chen Yeh, Jheng-Fong Jhong, James Huang, Yung Chang
    更新日期:2017-11-07
  • Functionalized graphene tagged polyurethanes for corrosion inhibitor and sustained drug delivery
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-04
    Dinesh K. Patel, Sudipta Senapati, Punita Mourya, Madan M Singh, Vinod Kumar Aswal, Biswajit Ray, Pralay Maiti

    Surface functionalization of graphene oxide with sulfonate group and subsequent grafting with polyurethane chains leads to the significant improvement in the properties of polymer and modified graphene as a filler. Modification of graphene oxide is revealed through spectroscopy while grafting of polymer chain over sulfonated graphene is confirmed through 1H-NMR and other techniques. Higher order of self-assembly phenomena is observed in nanohybrids as compared to pure polymer through greater interaction between polymer chain and sulfonated graphene. Significant improvement in corrosion inhibition phenomena is observed using nanohybrids at low concentration as compared to pure polymer indicating its superior efficiency as a corrosion inhibitor. Nanohybrids also exhibit better biocompatible nature in lower concentration of filler with considerable sustained release of drug vis-à-vis pure polymer suggest its potential to use as a biomaterial for tissue engineering applications.

    更新日期:2017-11-05
  • Self-assembling peptide nanofiber hydrogels for controlled ocular delivery of timolol maleate
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-04
    Christina Karavasili, Anastasia Komnenou, Orestis L. Katsamenis, Glykeria Charalampidou, Evangelia Kofidou, Dimitrios Andreadis, Sotirios Koutsopoulos, Dimitrios G. Fatouros

    The self-assembling peptides Ac-(RADA)4-CONH2 and Ac-(IEIK)3I-CONH2 which form hydrogels in physiological conditions were evaluated as carriers for ocular delivery of the β-blocker timolol maleate. Electron microscopy studies revealed that hydrogels contain nanofibers, whereas rheological studies showed that the Ac-(IEIK)3I-CONH2 self-assembles in a stiffer hydrogel compared with the Ac-(RADA)4-CONH2 peptide. The in vitro release and ex vivo permeation studies demonstrated controlled release and transport of the drug through the cornea, which depended on the self-assembling peptide sequence. In vivo studies in rabbits showed significant increase in the area under the concentration-time curve (AUC) after administration of the drug for the Ac-(RADA)4-CONH2 hydrogel compared to drug solution, whereas a sustained reduction of intraocular pressure for up to 24 h after instillation was achieved for both drug loaded hydrogels. Histological studies revealed good ocular tolerability upon application of the formulations, suggesting that self-assembling peptide hydrogels are promising systems for sustained ocular drug delivery.

    更新日期:2017-11-05
  • Biomimetic hyaluronic acid-lysozyme composite coating on AZ31 Mg alloy with combined antibacterial and osteoinductive activities
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-03
    Sankalp Agarwal, Mathieu Riffault, David A. Hoey, B Duffy, James Curtin, Swarna Jaiswal

    This study presents the covalent grafting of a hyaluronic acid-lysozyme (HA-LZ) composite onto corrosion resistant silane coated AZ31 Mg alloy via EDC-NHS coupling reactions. The HA-LZ composite coatings created a smooth and hydrophilic surface with the increased concentration of functional lysozyme complexed to the hyaluronic acid group. This was confirmed by the measurement of AFM, water contact angle, and quantification of hyaluronic acid and lysozyme. The colonisation of S.aureus on HA-LZ composite coated substrates was significantly reduced as compared to the hyaluronic acid, lysozyme coated and uncoated AZ31 controls. Such activity is due to the enhanced antibacterial activity of lysozyme component as observed from thw spread plate assay, propidium iodide staining and scanning electron microscopy. Furthermore, morphology of the osteoblast cells, alkaline phosphatase activity and DNA quantification studies demonstrated the improved biocompatibility and osteoinductive properties of HA-LZ coated substrates. This was verified by comparing with the lysozyme coated and uncoated AZ31 substrates in terms of cell adhesion, proliferation and differentiation of osteoblastic cells. Therefore, such multifunctional composite coatings with antibacterial and osteoinductive properties are promising can be potentially used for the surface modifications of orthopaedic implants.

    更新日期:2017-11-05
  • Bortezomib Increases the Cancer Therapeutic Efficacy of Poly(amino acid)–Doxorubicin
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-02
    Na Shen, Jian Jiang, Dawei Zhang, Guanyi Wang, Shixian Lv, Yanjie Jia, Zhaohui Tang, Xuesi Chen
    更新日期:2017-11-03
  • Facile Oriented Immobilization of Histidine-Tagged Proteins on Nonfouling Cobalt Polyphenolic Self-Assembly Surfaces
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-02
    Lulu Han, Qi Liu, Liwei Yang, Tong Ye, Zhien He, Lingyun Jia
    更新日期:2017-11-03
  • Differential Regulation of Extracellular Matrix Components Using Different Vitamin C Derivatives in Mono- and Coculture Systems
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-02
    Xiaoqing Zhang, Kyle G. Battiston, Craig A. Simmons, J. Paul Santerre
    更新日期:2017-11-02
  • An Injectable, Self-healing Chimeric Catechol-Fe(III) Hydrogel for Localized Combination Cancer Therapy
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-02
    Prabhu Srinivas Yavvari, Sanjay Pal, Sandeep Kumar, Animesh Kar, Anand Kumar Awasthi, Aaliya Naaz, Aasheesh Srivastava, Avinash Bajaj

    Conventional intravenous or oral administration of combination of chemotherapeutics display poor bioavailability and induce undesirable systemic toxicity. Therefore, localized delivery of such chemotherapeutic combinations using polymeric hydrogels is expected to help in enhancing drug efficacy and reducing systemic toxicity. In this manuscript, we have utilized a chitosan-catechol based hydrogel (CAT-Gel) assembled through catechol-Fe(III) coordinative interactions for localized combination therapy in murine lung and breast cancer models. CAT-Gel offers a unique blend of material properties such as injectability and self-healing along with useful biological attributes like their non-cytotoxic and non-hemolytic nature. The amphipathic nature of this hydrogel enabled us to incorporate a recipe of hydrophilic Doxorubicin hydrochloride (DOX) and hydrophobic Docetaxel (DTX) anticancer drugs. Rheology studies confirmed the self-healing nature of this chimeric hydrogel even after drug loading. CAT-Gel was retained for more than 40 days in mice upon subcutaneous injection. The sequential and sustained release of the entrapped DOX and DTX from the hydrogel resulted in synergistic therapeutic effect with increased median survival against murine lung and breast cancer models. Therefore, CAT-Gel provides a new coordinatively-assembled biocompatible scaffold for localized delivery of chemotherapeutic drugs.

    更新日期:2017-11-02
  • Synthesis and Characterization of Plug-and-Play Polyurethane Urea Elastomers as Biodegradable Matrixes for Tissue Engineering Applications
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-01
    Alysha P. Kishan, Thomas Wilems, Sahar Mohiuddin, Elizabeth M. Cosgriff-Hernandez
    更新日期:2017-11-01
  • A Polypropylene-Integrated Bilayer Composite Mesh with Bactericidal and Antiadhesive Efficiency for Hernia Operations
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-01
    Umran Aydemir Sezer, Vildan Sanko, Mehmet Gulmez, Elif Sayman, Basak Aru, Zehra Nur Yuksekdag, Ali Aktekin, Fugen Vardar Aker, Serdar Sezer
    更新日期:2017-11-01
  • Cell microenvironment pH sensing in 3D microgels using fluorescent carbon dots
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-11-01
    Anil Chandra, Neetu Singh

    We report here a 3D cell culture microgel based system containing carbon dots capable of sensing the pH changes in the cellular microenvironment. We have utilized a simple droplet based microfluidics methodology for encapsulating cells and fluorescent pH sensitive carbon dots in polyethyleneglycol microgels. Since, the microfluidics assembly is developed from simple components that can be modified easily to yield microgels of different size, composition and architecture; it can be utilized to develop complex 3D cell culture scaffolds of desired composition along with spatial control on the polymer composition. The synthesized pH sensitive carbon dots possess green fluorescence emission, which increases as the pH is lowered from neutral to acidic. Since the probe sensitivity to pH change is well within the physiologically relevant range (pH 5.8- pH 7.7) the probe can be used for detecting lowering of pH as the cells proliferate or undergo various biological processes. We demonstrate that the nanoprobes and the process of forming the microgel beads with nanoprobes and mammalian cells is biocompatible and the cells easily proliferate inside the microgels. The changes in pH as the mammalian cells grow in the microgels is easily monitored via fluorescence microscopy suggesting that the platform can be used to study time dependent changes in cellular microenvironment pH and can be easily utilized to monitor cellular growth, disease progression etc.

    更新日期:2017-11-01
  • Polymer Microneedle Mediated Local Aptamer Delivery for Blocking the Function of VEGF
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-10-31
    James Coyne, Brandon Davis, David Kauffman, Nan Zhao, Yong Wang

    Overexpression of proteins in the body can cause severe diseases and other physiological disturbances. The development of protein blockers and local delivery systems would offer opportunities for addressing the health problems caused by protein overexpression. Nucleic acid aptamers are an emerging class of ligands with the potential to block proteins effectively; however, little effort has been made in developing polymer systems for local aptamer delivery. In this work, polymer microneedles capable of delivering DNA aptamers locally to inhibit the function of vascular endothelial growth factor (VEGF) were developed and studied. The presence of anti-VEGF aptamer in the polymer matrix did not change the apparent mechanical strength of the microneedles. Once in contact with a physiological solution, the polymer microneedles quickly dissolved, generating a high concentration of anti-VEGF aptamer in the surrounding local microenvironment. Aptamer delivery by way of dissolving polymer microneedles in a tissue phantom reduced VEGF-mediated endothelial cell tube formation. Thus, aptamer-loaded polymer microneedles hold great potential as a therapeutic tool for the treatment of human diseases resulting from protein overexpression.

    更新日期:2017-10-31
  • Freeze-Dried Chitosan-Platelet-Rich Plasma Implants for Rotator Cuff Tear Repair: Pilot Ovine Studies
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-10-31
    Gabrielle Deprés-Tremblay, Anik Chevrier, Mark B Hurtig, Martyn Snow, Scott Rodeo, Michael D Buschmann
    更新日期:2017-10-31
  • Capturing Circulating Tumor Cells through a Combination of Hierarchical Nanotopography and Surface Chemistry
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-10-31
    Guang Yang, Xilin Li, Yang He, Xiang Xiong, Pu Wang, Shaobing Zhou
    更新日期:2017-10-31
  • Alternative SiO2 Surface Direct MDCK Epithelial Behavior
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-10-30
    Alan D. Covell, Zheng Zeng, Taylor Mabe, Jianjun Wei, Amy Adamson, Dennis R. LaJeunesse
    更新日期:2017-10-31
  • Nano-/Microfibrous Cotton-Wool-Like 3D Scaffold with Core–Shell Architecture by Emulsion Electrospinning for Skin Tissue Regeneration
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-10-30
    Pallabi Pal, Pavan Kumar Srivas, Prabhash Dadhich, Bodhisatwa Das, Dhrubajyoti Maulik, Santanu Dhara
    更新日期:2017-10-31
  • Hollow copper sulfide nanosphere-doxorubicin/graphene oxide core-shell nanocomposite for photothermo-chemotherapy
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-10-30
    Lu Han, Yanan Hao, Xing Wei, Xuwei Chen, Yang Shu, Jian-Hua Wang

    A novel core-shell nanostructure, hollow copper sulfide nanosphere-doxorubicin (DOX)/graphene oxide (GO) (CuS-DOX/GO), is constructed for the purpose of controlled drug delivery and improved photothermo-chemo therapeutic effect. The CuS-DOX/GO nanocomposite is configured by employing dual photothermal agents, where the core, hollow CuS nanoparticle, acts as delivery-carrier for doxorubicin, and the shell, PEGylated GO nanosheet, prohibits the leakage of drug. DOX can be efficiently loaded onto the hollow CuS nanoparticles, and its subsequent release from CuS-DOX/GO nanocomposite is triggered in a pH- and near-infrared light-dependent manner. Moreover, the integration of the two photothermal agents significantly improves the photothermal performance of this system. Ultimately, the combination of phototherapy and chemotherapy based on this system results in a much higher HeLa cell killing efficacy with respect to that for single chemotherapy mode, as demonstrated by in vitro cytotoxicity tests.

    更新日期:2017-10-30
  • Near-infrared light-triggered polymeric nanomicelles for cancer therapy and imaging
    ACS Biomater. Sci. Eng. (IF 3.234) Pub Date : 2017-10-30
    LEI LI, Xin Pang, Gang Liu

    Early diagnosis and efficient therapy are very important for cancer management. Among the various intelligent delivery systems, near-infrared (NIR) light-triggered polymeric nanomicelles (PNMs) have emerged as a promising approach for imaging and photoactive delivery. This platform can be used for spatially and temporally controlled remote release of therapeutic payload molecules under NIR light irradiation. This review discusses the design and characteristics of PNMs, as well as their recent application in imaging, drug delivery, and theranostics in photothermal therapy and photodynamic therapy. The challenges and potential of PNMs in biomedical applications are also highlighted.

    更新日期:2017-10-30
Some contents have been Reproduced with permission of the American Chemical Society.
Some contents have been Reproduced by permission of The Royal Society of Chemistry.
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