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The transporter PHO84/NtPT1 is a target of aluminum to affect phosphorus absorption in Saccharomyces cerevisiae and Nicotiana tabacum L. Metallomics (IF 3.4) Pub Date : 2023-11-22 Zhiwei Huang,Shixuan Zhang,Ranran Chen,Qian Zhu,Ping Shi,Yuhu Shen
The molecular mechanism of aluminum toxicity in biological systems is not completely understood. Saccharomyces cerevisiae is one of the most used model organisms in the study of environmental metal toxicity. Using an unbiased metallomic approach in yeast, we found that aluminum treatment caused phosphorus deprivation, and the lack of phosphorus increased as the pH of the environment decreased compared
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Characterization of genes involved in micronutrients and toxic metals detoxification in Brassica napus by genome-wide cDNA library screening. Metallomics (IF 3.4) Pub Date : 2023-11-21 Jia-Shi Peng,Xue-Jie Zhang,Jia-Ni Xiong,Ying Zhou,Wei-Li Wang,Si-Ying Chen,Da-Wei Zhang,Tian-Yu Gu
Stresses caused by deficiency/excess of mineral nutrients or of pollution of toxic metals have already become a primary factor in limiting crop production worldwide. Genes involved in minerals and toxic metals accumulation/tolerance could be potential candidates for improving crop plants with enhanced nutritional efficiency and environmental adaptability. In this study, we first generated a high-quality
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Diet, cellular, and systemic homeostasis control the cycling of potassium stable isotopes in endothermic vertebrates. Metallomics (IF 3.4) Pub Date : 2023-11-02 T Tacail,J Lewis,M Clauss,C D Coath,R Evershed,E Albalat,T R Elliott,T Tütken
The naturally occurring stable isotopes of potassium (41K/39K, expressed as δ41K) have the potential to make significant contributions to vertebrate and human biology. The utility of K stable isotopes is, however, conditioned by the understanding of the dietary and biological factors controlling natural variability of δ41K. This paper reports a systematic study of K isotopes in extant terrestrial endothermic
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Selenomethionine supplementation and expression of selenosugars, selenocysteine, and other selenometabolites in rat liver. Metallomics (IF 3.4) Pub Date : 2023-11-02 Katarzyna Bierla,Joanna Szpunar,Ryszard Lobinski,Roger A Sunde
Selenomethionine (SeMet) as a methionine analog can be incorporated into protein. In turkeys, we recently found that selenium (Se) as selenite is not metabolized to SeMet but rather to selenosugars (seleno-N-acetyl galactosamine) bound to protein as well as to selenocysteine (Sec) in selenoproteins. To characterize the metabolism of SeMet, we fed rats graded levels of SeMet from 0 to 5 µg Se/g in a
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Effect of graded levels of selenium supplementation as selenite on expression of selenosugars, selenocysteine, and other selenometabolites in rat liver. Metallomics (IF 3.4) Pub Date : 2023-11-02 Katarzyna Bierla,Joanna Szpunar,Ryszard Lobinski,Roger A Sunde
Using high pressure liquid chromatography (HPLC) coupled with selenium-specific inductively coupled plasma mass spectrometry (ICP-MS) and molecule specific (Orbitrap MS/MS) detection, we previously found that far more selenium (Se) is present as selenosugar (seleno-N-acetyl galactosamine) in Se-adequate turkey liver than is present as selenocysteine (Sec) in true selenoproteins, and that selenosugars
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Thallium hyperaccumulation status of the violets of the Allchar arsenic-thallium deposit (North Macedonia) confirmed through synchrotron µXRF imaging. Metallomics (IF 3.4) Pub Date : 2023-11-02 Ksenija Jakovljević,Tomica Mišljenović,Katerina Bačeva Andonovska,Guillaume Echevarria,Alan J M Baker,Dennis Brueckner,Antony van der Ent
The abandoned Allchar Mine in the Republic of North Macedonia is a globally unique deposit with the highest known grades of thallium (Tl) and arsenic (As) mineralization. We aimed to determine the distribution of As and Tl in whole dehydrated shoots of the three Viola taxa using synchrotron micro-X-ray fluorescence analysis. Additionally, soil and plant organ samples were collected from all three Viola
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Copper isotope ratios in serum do not track cancerous tumor evolution, but organ failure. Metallomics (IF 3.4) Pub Date : 2023-11-02 Emily Miaou,François L H Tissot
Relative to healthy controls, lighter copper isotopic compositions have been observed in the serum of breast cancer and end-stage liver disease patients, raising the possibility that Cu isotope ratios could be used as a tracer for disease progression. Here, we assess the potential of natural Cu isotopic variations (expressed as δ65Cu) as diagnostic tools for cancer progression and/or liver failure
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In vitro maturation of NiSOD reveals a role for cytoplasmic histidine in processing and metalation. Metallomics (IF 3.4) Pub Date : 2023-11-02 Priyanka Basak,Diane E Cabelli,Peter T Chivers,Erik R Farquhar,Michael J Maroney
The importance of cellular low molecular weight ligands in metalloenzyme maturation is largely unexplored. Maturation of NiSOD requires post-translational N-terminal processing of the proenzyme, SodN, by its cognate protease, SodX. Here we provide evidence for the participation of L-histidine in the protease-dependent maturation of nickel-dependent superoxide dismutase (NiSOD) from Streptomyces coelicolor
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Heme oxygenase-1 increases intracellular iron storage and suppresses inflammatory response of macrophages by inhibiting M1 polarization. Metallomics (IF 3.4) Pub Date : 2023-10-04 Xueyou Tang,Yunqin Li,Jing Zhao,Li Liang,Kang Zhang,Xiaofeng Zhang,Hong Yu,Huahua Du
Heme oxygenase-1 (HO-1) catalyzes the first and rate-limiting enzymatic step of heme degradation, producing carbon monoxide, biliverdin, and free iron. Most iron is derived from aged erythrocytes by the decomposition of heme, which happened mainly in macrophages. However, the role of HO-1 on iron metabolism and function of macrophage is unclear. The present study investigated the effect of HO-1 on
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Differentiated Zn(II) binding affinities in animal, plant, and bacterial metallothioneins define their zinc buffering capacity at physiological pZn. Metallomics (IF 3.4) Pub Date : 2023-10-04 Karolina Mosna,Kinga Jurczak,Artur Krężel
Metallothioneins (MTs) are small, Cys-rich proteins present in various but not all organisms, from bacteria to humans. They participate in zinc and copper metabolism, toxic metals detoxification, and protection against reactive species. Structurally, they contain one or multiple domains, capable of binding a variable number of metal ions. For experimental convenience, biochemical characterization of
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Dynamic elementomics of single-cell ICP-MS-derived signals in normal and calcium pump PMCA4-deficient mouse epididymal sperm during capacitation. Metallomics (IF 3.4) Pub Date : 2023-10-04 Bao Li Zhang,Ze Peng Zhang,Su Meng Shi,Hui Juan Shi,Patricia A DeLeon,Winnie Shum
Currently, clinical analysis of male infertility mainly relies on parameters of semen and sperm cells. However, the high diagnostic failure rates indicate that the current assessment methods are not sufficient and a new approach to evaluating sperm function still needs to be developed. Here we explored the feasibility of single-cell inductively coupled plasma mass spectrometry (sc-ICP-MS)-derived profiles
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Synchrotron X-rays reveal the modes of Fe binding and trace metal storage in the brown algae Laminaria digitata and Ectocarpus siliculosus. Metallomics (IF 3.4) Pub Date : 2023-10-04 Ana Mijovilovich,Peter Cloetens,Antonio Lanzirotti,Matt Newville,Gerd Wellenreuther,Puja Kumari,Christos Katsaros,Carl J Carrano,Hendrik Küpper,Frithjof C Küpper
Iron is accumulated symplastically in kelp in a non-ferritin core that seems to be a general feature of brown algae. Microprobe studies show that Fe binding depends on tissue type. The sea is generally an iron-poor environment and brown algae were recognized in recent years for having a unique, ferritin-free iron storage system. Kelp (Laminaria digitata) and the filamentous brown alga Ectocarpus siliculosus
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Evolution of Cd2+ and Cu+ binding in Helix pomatia metallothioneins. Metallomics (IF 3.4) Pub Date : 2023-10-04 Renato Valsecchi,Christian Baumann,Ardit Lila,Oliver Zerbe
Metallothioneins (MTs) are small proteins present in all kingdoms of life. Their high cysteine content enables them to bind metal ions, such as Zn2+, Cd2+, and Cu+, providing means for detoxification and metal homeostasis. Three MT isoforms with distinct metal binding preferences are present in the Roman Snail Helix pomatia. Here, we use nuclear magnetic resonance (NMR) to follow the evolution of Cd2+
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Inflammation alters iron distribution in bone and spleen in mice. Metallomics (IF 3.4) Pub Date : 2023-10-04 JuOae Chang,Melis Debreli Coskun,Jonghan Kim
Anemia of inflammation (or inflammation-associated anemia) decreases the quality of life in billions of patients suffering from various inflammatory diseases, such as infection, autoimmune diseases, and cancer, associated with a prolonged state of immune activation. While proper utilization of iron, a nutrient metal essential for erythropoiesis, is important for the prevention of anemia, the alteration
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Metallothionein-3 and carbonic anhydrase metalation properties with Zn(II) and Cd(II) change as a result of protein-protein interactions. Metallomics (IF 3.4) Pub Date : 2023-10-04 Amelia T Yuan,Martin J Stillman
Metallothioneins (MT) are regulators of the metals Zn(II) and Cu(I) and act as antioxidants in many organisms, including in humans. Isoform 3 (MT3) is expressed constitutively in central nervous tissue and has been shown to have additional biological functions, including the inhibition of neuronal growth, the regulation of apoptosis, and cytoskeleton modulation. To facilitate these functions, protein-protein
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Simultaneous quantification of Gadoxetic acid and Cisplatin in hepatocellular carcinomas using laser ablation-inductively coupled plasma-mass spectrometry. Metallomics (IF 3.4) Pub Date : 2023-10-04 Katharina Kronenberg,Julia Werner,Peter Bohrer,Katja Steiger,Rebecca Buchholz,Maximilian von Bremen-Kühne,Matthias Elinkmann,Philipp M Paprottka,Rickmer F Braren,Fabian K Lohöfer,Uwe Karst
The gadolinium-based contrast agent Gadoxetic acid and the platinum-based antitumor agent Cisplatin were quantitatively imaged in liver and liver cancer (hepatocellular carcinoma, HCC) tissue of rats by means of laser ablation-inductively coupled plasma-mass spectrometry. HCC bearing rats simultaneously received a tail vein injection of the hepatocyte-specific magnetic resonance imaging contrast agent
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Cu(I) binds to Zn7-MT2 via two parallel pathways. Metallomics (IF 3.4) Pub Date : 2023-09-05 Adyn Melenbacher,Martin J Stillman
Metallothionein proteins are essential for Cu(I) and Zn(II) homeostasis as well as heavy metal detoxification. The metallation properties of MT2 are of great interest due to their wide patterns of expression and correlation with multiple diseases including cancers, neurological disorders, and respiratory diseases. Use of isotopically pure 63Cu(I) and 68Zn(II) eliminates the complexity of the Cu, Zn-MT2
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Wilson disease-causing mutations in the carboxyl terminus of ATP7B regulates its localization and Golgi exit selectively in the unpolarized cells. Metallomics (IF 3.4) Pub Date : 2023-09-05 Kaustav Chakraborty,Santanu Das,Anusree Pal,Saptarshi Maji,Bhawana Rai,Arnab Gupta,Ashima Bhattacharjee
Mutational inactivation of the P-type Cu-ATPase ATP7B interferes with its cellular functions to varying extent leading to varied cellular phenotypes. Wilson's disease (WD) primarily affects organs composed of polarized/differentiated epithelial cells. Therefore, phenotypic variability might differ depending on the polarization/differentiation of the cells. The present study investigates the intracellular
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Repurposing sunscreen as an antibiotic: zinc-activated avobenzone inhibits methicillin-resistant Staphylococcus aureus. Metallomics (IF 3.4) Pub Date : 2023-09-05 Rachel M Andrews,Gretchen E Bollar,A Sophia Giattina,Alex G Dalecki,John R Wallace,Leah Frantz,Kayla Eschliman,Obdulia Covarrubias-Zambrano,Johnathan D Keith,Alexandra Duverger,Frederic Wagner,Frank Wolschendorf,Stefan H Bossmann,Susan E Birket,Olaf Kutsch
Methicillin-resistant Staphylococcus aureus (MRSA) is a major healthcare concern with associated healthcare costs reaching over ${\$}$1 billion in a single year in the USA. Antibiotic resistance in S. aureus is now observed against last line of defense antibiotics, such as vancomycin, linezolid, and daptomycin. Unfortunately, high throughput drug discovery approaches to identify new antibiotics effective
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Critical evaluation of cell lysis methods for metallodrug studies in cancer cells. Metallomics (IF 3.4) Pub Date : 2023-09-05 Mie Riisom,Stephen M F Jamieson,Christian G Hartinger
Intracellular accumulation studies are a key step in metallodrug development but often variable results are obtained. Therefore, we aimed here to investigate different protocols for efficient and reproducible lysis of cancer cells in terms of protein content in lysates and in cell uptake studies of the Ru anticancer complex [chlorido(8-oxyquinolinato)(η6-p-cymene)ruthenium(II)] ([Ru(cym)(HQ)Cl]). The
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High-energy interference-free K-lines synchrotron X-ray fluorescence microscopy of rare earth elements in hyperaccumulator plants. Metallomics (IF 3.4) Pub Date : 2023-09-05 Antony van der Ent,Dennis Brueckner,Kathryn M Spiers,Ken Vidar Falch,Gerald Falkenberg,Clément Layet,Wen-Shen Liu,Hong-Xiang Zheng,Marie Le Jean,Damien Blaudez
Synchrotron-based micro-X-ray fluorescence analysis (µXRF) is a nondestructive and highly sensitive technique. However, element mapping of rare earth elements (REEs) under standard conditions requires care, since energy-dispersive detectors are not able to differentiate accurately between REEs L-shell X-ray emission lines overlapping with K-shell X-ray emission lines of common transition elements of
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Chemical transformations of arsenic in the rhizosphere-root interface of Pityrogramma calomelanos and Pteris vittata. Metallomics (IF 3.4) Pub Date : 2023-08-01 Amelia Corzo Remigio,Hugh H Harris,David J Paterson,Mansour Edraki,Antony van der Ent
Pityrogramma calomelanos and Pteris vittata are cosmopolitan fern species that are the strongest known arsenic (As) hyperaccumulators, with potential to be used in the remediation of arsenic-contaminated mine tailings. However, it is currently unknown what chemical processes lead to uptake of As in the roots. This information is critical to identify As-contaminated soils that can be phytoremediated
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Human serum albumin as a copper source for anticancer thiosemicarbazones. Metallomics (IF 3.4) Pub Date : 2023-08-01 Martin Schaier,Enrico Falcone,Tomas Prstek,Bertrand Vileno,Sonja Hager,Bernhard K Keppler,Petra Heffeter,Gunda Koellensperger,Peter Faller,Christian R Kowol
Thiosemicarbazones (TSCs) are a class of biologically active compounds with promising anticancer activity. Their typical mechanism, especially of the clinically far developed representative Triapine, is chelation of iron (Fe), with the Fe-containing enzyme ribonucleotide reductase as primary intracellular target. However, for the subclass of terminally disubstituted, nanomolar-active derivatives like
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Interactions between chromium species and DNA in vitro and their potential role in the toxicity of hexavalent chromium. Metallomics (IF 3.4) Pub Date : 2023-08-01 R Mezencev,C Gibbons
Epidemiological and animal studies have supported the carcinogenicity of hexavalent chromium [Cr(VI)]; however, molecular changes responsible for the induction of cancer by Cr(VI) are not entirely understood. Numerous mechanistic studies suggested the role of oxidative stress and genotoxicity in Cr(VI)-mediated carcinogenesis; however, specific types of DNA damage have not yet been conclusively attributed
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A bioinformatic analysis of zinc transporters in intestinal Lactobacillaceae. Metallomics (IF 3.4) Pub Date : 2023-08-01 Uyen Huynh,Hazel N Nguyen,Brittany K Trinh,Joanna Elhaj,Melissa L Zastrow
As the second most abundant transition element and a crucial cofactor for many proteins, zinc is essential for the survival of all living organisms. To maintain required zinc levels and prevent toxic overload, cells and organisms have a collection of metal transport proteins for uptake and efflux of zinc. In bacteria, metal transport proteins are well defined for model organisms and many pathogens
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Zn deficiency disrupts Cu and S homeostasis in Chlamydomonas resulting in over accumulation of Cu and Cysteine. Metallomics (IF 3.4) Pub Date : 2023-07-10 Daniela Strenkert,Stefan Schmollinger,Yuntao Hu,Christian Hofmann,Kristen Holbrook,Helen W Liu,Samuel O Purvine,Carrie D Nicora,Si Chen,Mary S Lipton,Trent R Northen,Stephan Clemens,Sabeeha S Merchant
Growth of Chlamydomonas reinhardtii in zinc (Zn) limited medium leads to disruption of copper (Cu) homeostasis, resulting in up to 40-fold Cu over-accumulation relative to its typical Cu quota. We show that Chlamydomonas controls its Cu quota by balancing Cu import and export, which is disrupted in a Zn deficient cell, thus establishing a mechanistic connection between Cu and Zn homeostasis. Transcriptomics
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Regulation of Atp7a RNA contributes to differentiation-dependent Cu redistribution in skeletal muscle cells. Metallomics (IF 3.4) Pub Date : 2023-07-10 Thomas J Whitlow,Yu Zhang,Nathan Ferguson,Alexandra M Perez,Hemchandra Patel,Josephine A Link-Kemp,Ethan M Larson,Allison T Mezzell,Vinit C Shanbhag,Michael J Petris,Katherine E Vest
Cu (Cu) is essential for several biochemical pathways due to its role as a catalytic cofactor or allosteric regulator of enzymes. Its import and distribution are tightly controlled by transporters and metallochaperones and Cu homeostasis is maintained by balancing Cu uptake and export. Genetic diseases are caused by impaired Cu transporters CTR1, ATP7A, or ATP7B but little is known about the regulatory
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Revisiting the pro-oxidant activity of copper: interplay of ascorbate, cysteine, and glutathione. Metallomics (IF 3.4) Pub Date : 2023-07-10 Enrico Falcone,Francesco Stellato,Bertrand Vileno,Merwan Bouraguba,Vincent Lebrun,Marianne Ilbert,Silvia Morante,Peter Faller
Copper (Cu) is essential for most organisms, but it can be poisonous in excess, through mechanisms such as protein aggregation, trans-metallation, and oxidative stress. The latter could implicate the formation of potentially harmful reactive oxygen species (O2•-, H2O2, and HO•) via the redox cycling between Cu(II)/Cu(I) states in the presence of dioxygen and physiological reducing agents such as ascorbate
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Screening the complex biological behavior of late lanthanides through genome-wide interactions. Metallomics (IF 3.4) Pub Date : 2023-08-01 Roger M Pallares,Dahlia D An,Solene Hebert,Alex Loguinov,Michael Proctor,Jonathan A Villalobos,Kathleen A Bjornstad,Chris J Rosen,Christopher Vulpe,Rebecca J Abergel
Despite their similar physicochemical properties, recent studies have demonstrated that lanthanides can display different biological behaviors. Hence, the lanthanide series can be divided into three parts, namely early, mid, and late lanthanides, based on their interactions with biological systems. In particular, the late lanthanides demonstrate distinct, but poorly understood biological activity.
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Examining the inorganic elemental composition of lobster phyllosoma (Panulirus ornatus) with X-ray fluorescence microscopy. Metallomics (IF 3.4) Pub Date : 2023-06-01 Daniel R McDougall,Robert Deas,Daryl L Howard,Quinn P Fitzgibbon,Gregory G Smith,Andrew G Jeffs,Duncan J McGillivray
The ornate spiny rock lobster, Panulirus ornatus, is an attractive candidate for aquaculture. The larval stages of spiny lobsters, known as phyllosoma, are complex with many developmental stages. Very little is known about the inorganic element composition of phyllosoma. In this study, a novel method using synchrotron X-ray fluorescence microscopy (XFM) was applied to investigate the distributions
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Mono- and binuclear complexes of copper(II) with dimethylaminomethyl derivatives of 2-naphthol and 6-quinolinol: synthesis and in vitro study of antitumor properties. Metallomics (IF 3.4) Pub Date : 2023-06-01 Vnira R Akhmetova,El'miraM Galimova,Ekaterina S Mescheryakova,Lilya U Dzhemileva,Usein M Dzhemilev,Vladimir A D'yakonov
1-(Dimethylamino)methyl-6-quinolinol scaffold, a structural moiety of the molecule of anticancer drug topotecan, was modified into copper-containing products to study cytotoxic properties. New mononuclear and binuclear Cu(II) complexes with 1-(N,N-dimethylamino)methyl-6-quinolinol were synthesized for the first time. The same way Cu(II) complexes with 1-(dimethylamino)methyl-2-naphtol ligand were synthesized
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Physiological responses of plants to in vivo X-ray damage from X-ray fluorescence measurements: insights from anatomical, elemental, histochemical, and ultrastructural analyses. Metallomics (IF 3.4) Pub Date : 2023-06-01 Gabriel Sgarbiero Montanha,João Paulo Rodrigues Marques,Eduardo Santos,Michael W M Jones,Hudson Wallace Pereira de Carvalho
X-ray fluorescence spectroscopy (XRF) is a powerful technique for the in vivo assessment of plant tissues. However, the potential X-ray exposure damages might affect the structure and elemental composition of living plant tissues, leading to artefacts in the recorded data. Herein, we exposed in vivo soybean (Glycine max (L.) Merrill) leaves to several X-ray doses through a polychromatic benchtop microprobe
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Lanthanum carbonate, a potent and selective phosphate binder, is transported and absorbed mainly via M cells in gastrointestinal tract. Metallomics (IF 3.4) Pub Date : 2023-06-01 Hui-Xia Huang,Hui-Xue Liu,Xiao-Gai Yang
This study aimed to investigate the transportation and absorption mechanism of lanthanum carbonate [La2(CO3)3] through the gastrointestinal (GI) tract using in vitro and in vivo models. The results demonstrated that La2(CO3)3 can be dissolved in gastric fluids and precipitated into lanthanum phosphate as the main transformed specie in intestinal fluid. Using Caco-2 cell monoculture and Caco-2/Raji
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Mitochondria-targeted cyclometalated iridium-β-carboline complexes as potent non-small cell lung cancer therapeutic agents. Metallomics (IF 3.4) Pub Date : 2023-06-01 Jincan Chen,Xinhua Guo,Dunhui Li,Hong Tang,Jie Gao,Wenzhu Yu,Xufeng Zhu,Zirong Sun,Zunnan Huang,Lanmei Chen
Natural products and metals play a crucial role in cancer research and the development of antitumor drugs. We designed and synthesized three new carboline-based cyclometalated iridium complexes [Ir(C-N)2(PPβC)](PF6), where PPβC = N-(1,10-phenanthrolin-5-yl)-1-phenyl-9H-pyrido[3,4-b]indole-3-carboxamide, C-N = 2-phenylpyridine (ppy, Ir1), 2-(2,4-difluorophenyl) pyridine (dfppy, Ir2), 7,8-benzoquinoline
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Stable potassium isotope distribution in mouse organs and red blood cells: implication for biomarker development. Metallomics (IF 3.4) Pub Date : 2023-07-10 Meng-Meng Cui,Frédéric Moynier,Ben-Xun Su,Wei Dai,Yan Hu,Dimitri Rigoussen,Brandon Mahan,Marie Le Borgne
Potassium (K) is an essential electrolyte for cellular functions in living organisms, and disturbances in K+ homeostasis could lead to various chronic diseases (e.g. hypertension, cardiac disease, diabetes, and bone health). However, little is known about the natural distribution of stable K isotopes in mammals and their application to investigate bodily homeostasis and/or as biomarkers for diseases
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Addressing uncertainties in correlative imaging of exogenous particles with the tissue microanatomy with synchronous imaging strategies. Metallomics (IF 3.4) Pub Date : 2023-06-01 Alexander P Morrell,Richard A Martin,Helen M Roberts,Hiram Castillo-Michel,J Frederick W Mosselmans,Kalotina Geraki,Adrian T Warfield,Paul Lingor,Wasif Qayyum,Daniel Graf,Maria Febbraio,Owen Addison
Exposure to exogenous particles is of increasing concern to human health. Characterizing the concentrations, chemical species, distribution, and involvement of the stimulus with the tissue microanatomy is essential in understanding the associated biological response. However, no single imaging technique can interrogate all these features at once, which confounds and limits correlative analyses. Developments
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ZnT1 induces a crosstalk between T-type and L-type calcium channels through interactions with Raf-1 kinase and the calcium channel β2 subunit. Metallomics (IF 3.4) Pub Date : 2023-06-01 Merav Mor,Ofer Beharier,David I Cook,Craig R Campbell,Levi A Gheber,Amos Katz,Arie Moran,Yoram Etzion
ZnT1 is a major zinc transporter that regulates cellular zinc homeostasis. We have previously shown that ZnT1 has additional functions that are independent of its activity as a Zn2+ extruder. These include inhibition of the L-type calcium channel (LTCC) through interaction with the auxiliary β-subunit of the LTCC and activation of the Raf-ERK signaling leading to augmented activity of the T-type calcium
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Chemical-genomic profiling identifies genes that protect yeast from aluminium, gallium, and indium toxicity. Metallomics (IF 3.4) Pub Date : 2023-06-01 Yves Schulze,Payam Ghiaci,Liqian Zhao,Marc Biver,Jonas Warringer,Montserrat Filella,Markus J Tamás
Aluminium, gallium, and indium are group 13 metals with similar chemical and physical properties. While aluminium is one of the most abundant elements in the Earth's crust, gallium and indium are present only in trace amounts. However, the increased use of the latter metals in novel technologies may result in increased human and environmental exposure. There is mounting evidence that these metals are
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Selenium deficiency causes oxidative stress and activates inflammation, apoptosis, and necroptosis in the intestine of weaned calves. Metallomics (IF 3.4) Pub Date : 2023-06-01 Lei Lei,Mu Jing,Zheng Yingce,Zhang Pei,Liu Yun
Selenium performs a variety of biological functions in organisms, including antioxidant and anti-inflammatory effects. This study investigated how selenium deficiency affects weaned calves' intestines. According to Inductively coupled plasma mass spectrometry (ICP-MS) analysis of intestinal selenium concentrations in calves, the Se-D group had a significantly lower concentration of selenium. Hematoxylin-eosin
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The connection of α- and β-domains in mammalian metallothionein-2 differentiates Zn(II) binding affinities, affects folding, and determines zinc buffering properties. Metallomics (IF 3.4) Pub Date : 2023-06-01 Avinash Kumar Singh,Adam Pomorski,Sylwia Wu,Manuel D Peris-Díaz,Hanna Czepczyńska-Krężel,Artur Krężel
Mammalian metallothioneins (MTs) are small Cys-rich proteins involved in Zn(II) and Cu(I) homeostasis. They bind seven Zn(II) ions in two distinct β- and α-domains, forming Zn3Cys9 and Zn4Cys11 clusters, respectively. After six decades of research, their role in cellular buffering of Zn(II) ions has begun to be understood recently. This is because of different affinities of bound ions and the proteins'
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From methodological limitations to the function of metallothioneins - a guide to approaches for determining weak, moderate, and tight affinity zinc sites. Metallomics (IF 3.4) Pub Date : 2023-05-02 Adam Pomorski,Agnieszka Drozd,Anna Kocyła,Artur Krężel
Mammalian metallothioneins (MTs) are small cysteine-rich proteins whose primary role is participation in zinc and copper homeostasis. Ever since their discovery, MTs have been investigated in terms of metal-binding affinity. The initial concept of seven Zn(II) ions (Zn7MT) bound with the same, undifferentiated low-picomolar affinity in the α and β domains prevailed for many years and derived from spectroscopic
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Zinc trafficking: 1,10-phenanthroline, glutathione, and other metal binding ligands form adducts with proteomic Zn2. Metallomics (IF 3.4) Pub Date : 2023-05-02 Kaniz Fatema,Eric Lund,David H Petering
Hypotheses were tested that the proteome of pig kidney LLC-PK1 cells (i) contains Zn-proteins that react with a diversity of native and pharmacologically active metal-binding ligands to form ternary complexes and (ii) includes proteins that bind Zn2+ nonspecifically and together form ternary adducts with a variety of metal-binding agents. The method to observe ternary complex formation with Zn-proteins
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Atp7b deficiency induces zebrafish eye developmental defects. Metallomics (IF 3.4) Pub Date : 2023-05-02 You Wu,Wenye Liu,Lingya Li,Zhipeng Tai,BaoXiang Gao,Jing-Xia Liu
As a copper (Cu) transport ATPase, ATP7B plays an important role in maintaining Cu homeostasis in the body and its dysfunction is associated with retinal disease. How ATP7B dysfunction and the subsequent Cu overload induce retinal damage, however, are unknown. Here, we show that atp7b-/- homozygous zebrafish larvae are insensitive to light stimulation, with a reduction in retinal cells but normal like
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Ferrocenoyl-substituted quinolinone and coumarin as organometallic inhibitors of SARS-CoV-2 3CLpro main protease. Metallomics (IF 3.4) Pub Date : 2023-05-02 Dominic Graf,Nikolas Farn,Jonas Klopf,Mahniya Hojjati,Ulrich Schatzschneider
The 3-chymotrypsin-like protease 3CLpro from severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is a potential target for antiviral drug development. In this work, three organometallic ferrocene-modified quinolinones and coumarins were compared to their benzoic acid ester analogues with regard to inhibition of 3CLpro using an HPLC-based assay with a 15mer model peptide as the substrate. In
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Expression pattern and prognostic implication of zinc homeostasis-related genes in acute myeloid leukemia. Metallomics (IF 3.4) Pub Date : 2023-05-02 Bo Zhu,Chunhao Yang,Longshuo Sun,Zhonghua Li,Jiahuang Li,Zi-Chun Hua
Zinc homeostasis is regulated by the SLC39A/ZIP, SLC30A/ZnT, and metallothionein (MT) protein families. The association of zinc homeostasis with acute myeloid leukemia (AML) is unclear. We previously demonstrated that zinc depletion by TPEN triggers apoptosis in NB4 AML cells with the degradation of PML-RARα oncoprotein, suggesting that zinc homeostasis may be associated with AML. The primary aim of
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Cisplatin reacts with the RING finger domain of RNF11 and interferes with the protein functions. Metallomics (IF 3.4) Pub Date : 2023-04-03 Yu Wang,Siming Yuan,Kaiming Cao,Yangzhong Liu
Protein reactions play important roles in the mechanism of action of cisplatin. In this work, we found that cisplatin is highly reactive to the RING finger domain of RNF11, a key protein involved in tumorigenesis and metastasis. The results show that cisplatin binds to RNF11 at the zinc coordination site and leads to zinc ejection from the protein. The formation of S-Pt(II) coordination and Zn(II)
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SLC30A10 manganese transporter in the brain protects against deficits in motor function and dopaminergic neurotransmission under physiological conditions. Metallomics (IF 3.4) Pub Date : 2023-04-03 Cherish A Taylor,Stephanie M Grant,Thomas Jursa,Ashvini Melkote,Rebecca Fulthorpe,Michael Aschner,Donald R Smith,Rueben A Gonzales,Somshuvra Mukhopadhyay
Loss-of-function mutations in SLC30A10 induce hereditary manganese (Mn)-induced neuromotor disease in humans. We previously identified SLC30A10 to be a critical Mn efflux transporter that controls physiological brain Mn levels by mediating hepatic and intestinal Mn excretion in adolescence/adulthood. Our studies also revealed that in adulthood, SLC30A10 in the brain regulates brain Mn levels when Mn
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Knockdown of the SELENOK gene induces ferroptosis in cervical cancer cells. Metallomics (IF 3.4) Pub Date : 2023-04-03 Anwar Abdurahman,Yu Li,Shi-Zheng Jia,Xin-Wen Xu,Shu-Jing Lin,Pei Ouyang,Zhi Jun He,Zhong-Hao Zhang,Qiong Liu,Ying Xu,Guo-Li Song
Selenoprotein K (SELENOK) is one of the endoplasmic reticulum (ER) proteins that mainly functions in the regulation of ER stress, calcium flux, and antioxidant defense. Reactive oxygen species (ROS) is one of the key indicators of ferroptosis, and SELENOK inhibition could disrupt ROS balance, and consequently might cause ferroptosis. However, there are no previous studies about the mechanism of SELENOK
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Antitumor potential of platinum(II) complexes of selenium donor ligands. Metallomics (IF 3.4) Pub Date : 2023-04-03 Saeed Ahmad,Anvarhusein A Isab,Abdul Rahman Al-Arfaj
Platinum(II) coordination compounds are widely applied in clinics as anticancer drugs. In this review, we provide a summary of the reports on cytotoxic properties of platinum(II) complexes of selenium donor ligands along with a brief description of their structural features. It has been observed that the platinum(II) complexes of selenones and selenoethers display reasonable antitumor properties and
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Insights into the binding of Ag ions with SilE model peptides: an NMR and MS coupled approach. Metallomics (IF 3.4) Pub Date : 2023-04-03 Gabriele Antonio Zingale,Valentina Oliveri,Giuseppe Grasso
The diffuse and renewed use of silver as antimicrobial agent has caused the development of resistance to silver ions in some bacterial strains, posing a serious threat for health systems. In order to cast light on the mechanistic features of resistance, here, we aimed to understand how silver interacts with the periplasmic metal-binding protein SilE which is engaged in bacterial silver detoxification
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Combining ultrafiltration and diffusive gradients in thin films techniques for speciation/fractionation of Cu and Zn in cytosol of liver of Nile tilapia (Oreochromis niloticus). Metallomics (IF 3.4) Pub Date : 2023-04-03 Hendryk Gemeiner,Amauri Antonio Menegário,Carlos Eduardo Eismann,Lucas Pellegrini Elias,Jorge Henrique Pedrobom,Thiago de Araujo Dourado,Hung Kiang Chang,Fabiano Tomazini da Conceição,Rodrigo Braga Moruzzi
This work aims to evaluate the size and lability of Cu and Zn bound to proteins in the cytosol of fish liver of Oreochromis niloticus by employing solid-phase extraction (SPE), diffusive gradients in thin films (DGT), and ultrafiltration (UF). SPE was carried out using Chelex-100. DGT containing Chelex-100 as binding agent was employed. Analyte concentrations were determined by ICP-MS. Total Cu and
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Coupling metal stable isotope compositions and X-ray absorption spectroscopy to study metal pathways in soil-plant systems: a mini review. Metallomics (IF 3.4) Pub Date : 2023-04-03 Anne Marie Aucour,Géraldine Sarret,Hester Blommaert,Matthias Wiggenhauser
Excess and limited trace metal contents in soils and plants can limit crop yields and pose a risk for the environment and human health. This mini-review reports on the emerging approach of combining X-ray absorption spectroscopy (XAS) with isotope analyses to improve the understanding of metal speciation and dynamics in soil-plant systems. In soils and their components, shifts in isotope compositions
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The influence of physiological and lifestyle factors on essential mineral element isotopic compositions in the human body: implications for the design of isotope metallomics research. Metallomics (IF 3.4) Pub Date : 2023-03-06 Kaj V Sullivan,Rebekah E T Moore,Frank Vanhaecke
In the last 20 years, the application of high-precision isotopic analysis of essential mineral elements (Mg, K, Ca, Fe, Cu, and Zn) to biomedicine (sometimes referred to as isotope metallomics) has revealed that their stable isotopic compositions are altered by the metal dysregulation that is fundamental to the pathogenesis of many cancers and other diseases. Despite many published works showing the
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Calcium isotopes as a biomarker for vascular calcification in chronic kidney disease. Metallomics (IF 3.4) Pub Date : 2023-03-06 Anthony Dosseto,Kelly Lambert,Hicham I Cheikh Hassan,Andrew Fuller,Addison Borst,Florian Dux,Maureen Lonergan,Theo Tacail
Calcium balance is abnormal in adults with chronic kidney disease (CKD) and is associated with the development of vascular calcification. It is currently not routine to screen for vascular calcification in CKD patients. In this cross-sectional study, we investigate whether the ratio of naturally occurring calcium (Ca) isotopes, 44Ca and 42Ca, in serum could be used as a noninvasive marker of vascular
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Chryseochelins-structural characterization of novel citrate-based siderophores produced by plant protecting Chryseobacterium spp. Metallomics (IF 3.4) Pub Date : 2023-03-06 Karoline Rehm,Vera Vollenweider,Shaohua Gu,Ville-Petri Friman,Rolf Kümmerli,Zhong Wei,Laurent Bigler
Bacteria secrete siderophores whose function is to acquire iron. In recent years, the siderophores of several Chryseobacterium species were shown to promote the health and growth of various plants such as tomato or rice. However, the chemical nature of Chryseobacterium siderophores remained unexplored despite great interest. In this work, we present the purification and structure elucidation by nuclear
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Droplet microfluidic chip-ICP-MS-based single-cell analysis for study of cellular behavior of macrophages to thimerosal. Metallomics (IF 3.4) Pub Date : 2023-04-03 Junrong Ma,Man He,Beibei Chen,Bin Hu
Thimerosal (THI) is widely used as an antimicrobial preservative, but can hydrolyze to ethylmercury, causing potentially neurotoxicity. In this work, a THP-1 cell line was used to investigate the biological behavior of THI. An on-line droplet microfluidic chip system combined with time-resolved inductively coupled plasma mass spectrometry was used to quantify Hg in single THP-1 cells. The cellular
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Roles of glutathione peroxidase 4 on the mercury-triggered ferroptosis in renal cells: implications for the antagonism between selenium and mercury. Metallomics (IF 3.4) Pub Date : 2023-03-06 Jiahao Chen,Minghao Ma,Ruixia Wang,Ming Gao,Ligang Hu,Sijin Liu,Ming Xu
Understanding of how mercury species cause cellular impairments at the molecular level is critical for explaining the detrimental effects of mercury exposure on the human body. Previous studies have reported that inorganic and organic mercury compounds can induce apoptosis and necrosis in a variety of cell types, but more recent advances reveal that mercuric mercury (Hg2+) and methylmercury (CH3Hg+)
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Toxicity and assimilation of cellulosic copper nanoparticles require α-arrestins in S. cerevisiae. Metallomics (IF 3.4) Pub Date : 2023-03-06 Ni Putu Dewi Nurmalasari,Matthew J Winans,Katelyn Perroz,Victoria R Bovard,Robert Anderson,Steve Smith,Jennifer E G Gallagher
The increased use of antimicrobial compounds such as copper into nanoparticles changes how living cells interact with these novel materials. The increased use of antimicrobial nanomaterials combats infectious disease and food spoilage. Fungal infections are particularly difficult to treat because of the few druggable targets, and Saccharomyces cerevisiae provides an insightful model organism to test
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Copper homeostasis and the ubiquitin proteasome system. Metallomics (IF 3.4) Pub Date : 2023-03-06 Bichao Zhang,Richard Burke
Copper is involved in many physiological pathways and important biological processes as a cofactor of several copper-dependent enzymes. Given the requirement for copper and its potential toxicity, intracellular copper levels are tightly controlled. Disturbances of human copper homeostasis are characterized by disorders of copper overload (Wilson's disease) or copper deficiency (Menkes disease). The
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