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Pipetting Hand, Together ACS Synth. Biol. (IF 4.7) Pub Date : 2024-03-15 Kwanwoo Shin
Scientific progress relies on the maxim that scientific inquiry knows no bounds: new results and insights are not dependent on the location of the scientists who conducted the work. However, there is no similar guarantee that researchers in disparate locations will have equitable opportunities to participate in science. During a visit to the chemistry department at the Royal University of Phnom Penh
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A Yeast Modular Cloning (MoClo) Toolkit Expansion for Optimization of Heterologous Protein Secretion and Surface Display in Saccharomyces cerevisiae ACS Synth. Biol. (IF 4.7) Pub Date : 2024-03-14 Nicola M. O’Riordan, Vanja Jurić, Sarah K. O’Neill, Aoife P. Roche, Paul W. Young
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Retron Library Recombineering: Next Powerful Tool for Genome Editing after CRISPR/Cas ACS Synth. Biol. (IF 4.7) Pub Date : 2024-03-13 Navdeep Kaur, Pratap Kumar Pati
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Cell-Free Protein Expression in Polymer Materials ACS Synth. Biol. (IF 4.7) Pub Date : 2024-03-11 Marilyn S. Lee, Jennifer A. Lee, John R. Biondo, Jeffrey E. Lux, Rebecca M. Raig, Pierce N. Berger, Casey B. Bernhards, Danielle L. Kuhn, Maneesh K. Gupta, Matthew W. Lux
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Ensemble Detection of DNA Engineering Signatures ACS Synth. Biol. (IF 4.7) Pub Date : 2024-03-12 Aaron Adler, Joel S. Bader, Brian Basnight, Benjamin W. Booth, Jitong Cai, Elizabeth Cho, Joseph H. Collins, Yuchen Ge, John Grothendieck, Kevin Keating, Tyler Marshall, Anton Persikov, Helen Scott, Roy Siegelmann, Mona Singh, Allison Taggart, Benjamin Toll, Kenneth H. Wan, Daniel Wyschogrod, Fusun Yaman, Eric M. Young, Susan E. Celniker, Nicholas Roehner
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Engineered Saccharomyces cerevisiae as a Biosynthetic Platform of Nucleotide Sugars ACS Synth. Biol. (IF 4.7) Pub Date : 2024-03-11 Samantha A. Crowe, Xixi Zhao, Fei Gan, Xiaoyue Chen, Graham A. Hudson, Maria C. T. Astolfi, Henrik V. Scheller, Yuzhong Liu, Jay D. Keasling
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Discovery of Latent Cannabichromene Cyclase Activity in Marine Bacterial Flavoenzymes ACS Synth. Biol. (IF 4.7) Pub Date : 2024-03-08 Anna C. Love, Trevor N. Purdy, Felix M. Hubert, Ella J. Kirwan, Darren C. Holland, Bradley S. Moore
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Genome Engineering by RNA-Guided Transposition for Anabaena sp. PCC 7120 ACS Synth. Biol. (IF 4.7) Pub Date : 2024-03-06 Sergio Arévalo, Daniel Pérez Rico, Dolores Abarca, Laura W. Dijkhuizen, Cristina Sarasa-Buisan, Peter Lindblad, Enrique Flores, Sandra Nierzwicki-Bauer, Henriette Schluepmann
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Efficient Mutasynthesis of “Non-Natural” Antitubercular Ilamycins with Low Cytotoxicity ACS Synth. Biol. (IF 4.7) Pub Date : 2024-03-05 Yuyang Wang, Jianqiao He, Md Shah Alam, Fang Wang, Zhuo Shang, Yingying Chen, Changli Sun, Ziwen Lu, Yamin Gao, Tianyu Zhang, Jianhua Ju, Junying Ma
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Validation of Cell-Free Protein Synthesis Aboard the International Space Station ACS Synth. Biol. (IF 4.7) Pub Date : 2024-03-05 Selin Kocalar, Bess M. Miller, Ally Huang, Emily Gleason, Kathryn Martin, Kevin Foley, D. Scott Copeland, Michael C. Jewett, Ezequiel Alvarez Saavedra, Sebastian Kraves
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Enzymatic Assembly of Small Synthetic Genes with Repetitive Elements ACS Synth. Biol. (IF 4.7) Pub Date : 2024-03-04 Michael T. A. Nguyen, Martin Vincent Gobry, Néstor Sampedro Vallina, Georgios Pothoulakis, Ebbe Sloth Andersen
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Progress in Engineering Synthetic Cells and Cell-Free Systems ACS Synth. Biol. (IF 4.7) Pub Date : 2024-03-02 Marileen Dogterom, Neha P. Kamat, Michael C. Jewett, Katarzyna P. Adamala
What defines life, and how do living systems distinguish themselves from nonliving entities? Can life be fabricated from inert molecules? Those questions have been driving the rapid growth of a new area of synthetic biology: a groundbreaking scientific field centered on the construction of synthetic cells. This ambitious initiative aims to uncover deep insights into the fundamental principles of life
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Genetic Parts and Enabling Tools for Biocircuit Design ACS Synth. Biol. (IF 4.7) Pub Date : 2024-03-01 Felipe Buson, Yuanli Gao, Baojun Wang
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An Efficient Method for the Production of High-Purity Bioinspired Large Unilamellar Vesicles ACS Synth. Biol. (IF 4.7) Pub Date : 2024-02-29 Meline Macher, Amelie Obermeier, Sebastian Fabritz, Massimo Kube, Hannah Kempf, Hendrik Dietz, Ilia Platzman, Joachim P. Spatz
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Comprehensive Parameter Space Mapping of Cell Cycle Dynamics under Network Perturbations ACS Synth. Biol. (IF 4.7) Pub Date : 2024-02-29 Zhengda Li, Shiyuan Wang, Meng Sun, Minjun Jin, Daniel Khain, Qiong Yang
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Engineering Brassica Crops to Optimize Delivery of Bioactive Products Postcooking ACS Synth. Biol. (IF 4.7) Pub Date : 2024-02-27 Collin R. Barnum, Myeong-Je Cho, Kasey Markel, Patrick M. Shih
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Subunits of an E3 Ligase Complex as Degrons for Efficient Degradation of Cytosolic, Nuclear, and Membrane Proteins ACS Synth. Biol. (IF 4.7) Pub Date : 2024-02-26 Anže Verbič, Tina Lebar, Arne Praznik, Roman Jerala
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Codon-tRNA Coadaptation Bias for Identifying Strong Native Promoters in Komagataella phaffii ACS Synth. Biol. (IF 4.7) Pub Date : 2024-02-21 Louise La Barbera Kastberg, Mykhaylo S. Petrov, Tomas Strucko, Michael K. Jensen, Christopher T. Workman
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Light-Mediated Enhancement of Glucose-Stimulated Insulin Release of Optogenetically Engineered Human Pancreatic Beta-Cells ACS Synth. Biol. (IF 4.7) Pub Date : 2024-02-20 Zijing Chen, Demetrios M. Stoukides, Emmanuel S. Tzanakakis
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Highly Parallelized Construction of DNA from Low-Cost Oligonucleotide Mixtures Using Data-Optimized Assembly Design and Golden Gate ACS Synth. Biol. (IF 4.7) Pub Date : 2024-02-20 Sean Lund, Vladimir Potapov, Sean R. Johnson, Jackson Buss, Nathan A. Tanner
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Combinatorial Biosynthesis of 3-O-Carbamoylmaytansinol by Rational Engineering of the Tailoring Steps of Ansamitocins ACS Synth. Biol. (IF 4.7) Pub Date : 2024-02-20 Qingqing Liu, Yu Wang, Xin Xia, Zhongyue Li, Yaoyao Li, Yuemao Shen, Haoxin Wang
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LanTERN: A Fluorescent Sensor That Specifically Responds to Lanthanides ACS Synth. Biol. (IF 4.7) Pub Date : 2024-02-20 Ethan M. Jones, Yang Su, Chris Sander, Quincey A. Justman, Michael Springer, Pamela A. Silver
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Development of the Static and Dynamic Gene Expression Regulation Toolkit in Pseudomonas chlororaphis ACS Synth. Biol. (IF 4.7) Pub Date : 2024-02-20 Sheng-Jie Yue, Zheng Zhou, Peng Huang, Yi-Chen Wei, Sheng-Xuan Zhan, Tong-Tong Feng, Ji-Rui Liu, Hao-Cheng Sun, Wei-Shang Han, Zhao-Long Xue, Zi-Xin Yan, Wei Wang, Xue-Hong Zhang, Hong-Bo Hu
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Design and Model-Driven Analysis of Synthetic Circuits with the Staphylococcus aureus Dead-Cas9 (sadCas9) as a Programmable Transcriptional Regulator in Bacteria ACS Synth. Biol. (IF 4.7) Pub Date : 2024-02-19 Davide De Marchi, Roman Shaposhnikov, Samy Gobaa, Daniele Pastorelli, Gregory Batt, Paolo Magni, Lorenzo Pasotti
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Correction to “Engineering a Novel Acetyl-CoA Pathway for Efficient Biosynthesis of Acetyl-CoA-Derived Compounds” ACS Synth. Biol. (IF 4.7) Pub Date : 2024-02-18 Mengzhen Nie, Jingyu Wang, Kechun Zhang
The original funding number in the acknowledgments for the National Natural Science Foundation of China should be corrected to the Grant No. 22078267. This corrected Acknowledgments section is published with this Correction. The authors acknowledge the support from the National Natural Science Foundation of China (Grant No. 22078267), Westlake University-Muyuan Joint Research Institute, the Westlake
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Gene Editing of Candida glycerinogenes by Designed Toxin–Antitoxin Cassette ACS Synth. Biol. (IF 4.7) Pub Date : 2024-02-16 Wen Lv, Xinyao Lu, Bin Zhuge, Hong Zong
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A Ribulose-5-phosphate Shunt from the Calvin–Benson Cycle to Methylerythritol Phosphate Pathway for Enhancing Photosynthetic Terpenoid Production ACS Synth. Biol. (IF 4.7) Pub Date : 2024-02-16 Jie Zhou, Suxian Xu, Hu Li, Huachao Xi, Wenbo Cheng, Chen Yang
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Carving out a Glycoside Hydrolase Active Site for Incorporation into a New Protein Scaffold Using Deep Network Hallucination ACS Synth. Biol. (IF 4.7) Pub Date : 2024-02-15 Anders Lønstrup Hansen, Frederik Friis Theisen, Ramon Crehuet, Enrique Marcos, Nushin Aghajari, Martin Willemoës
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Artificial Methylotrophic Cells via Bottom-Up Integration of a Methanol-Utilizing Pathway ACS Synth. Biol. (IF 4.7) Pub Date : 2024-02-15 Ke Wang, Xueqing Liu, Kevin K.Y. Hu, Victoria S. Haritos
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CRISPR-Based Multiplex Detection of Human Papillomaviruses for One-Pot Point-of-Care Diagnostics ACS Synth. Biol. (IF 4.7) Pub Date : 2024-02-13 Ahmed Ghouneimy, Zahir Ali, Rashid Aman, Wenjun Jiang, Mustapha Aouida, Magdy Mahfouz
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Establishing CRISPRi for Programmable Gene Repression and Genome Evolution in Cupriavidus necator ACS Synth. Biol. (IF 4.7) Pub Date : 2024-02-13 Zhijiao Wang, Haojie Pan, Sulin Ni, Zhongjian Li, Jiazhang Lian
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Equipping Saccharomyces cerevisiae with an Additional Redox Cofactor Allows F420-Dependent Bioconversions in Yeast ACS Synth. Biol. (IF 4.7) Pub Date : 2024-02-12 Misun Lee, Marco W. Fraaije
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Characterization and Application of a Stationary Phase Promoter Library from Serratia marcescens ACS Synth. Biol. (IF 4.7) Pub Date : 2024-02-10 Di Liu, Linbo Gou, Tingting Sun, Jie Liu, Tai-Ping Fan, Huaxiang Deng, Yujie Cai
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LactoSpanks: A Collection of IPTG Inducible Promoters for the Commensal Lactic Acid Bacteria Lactobacillus gasseri ACS Synth. Biol. (IF 4.7) Pub Date : 2024-02-09 Elsa Fristot, Guillaume Cambray, Jerome Bonnet
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OptoREACT: Optogenetic Receptor Activation on Nonengineered Human T Cells ACS Synth. Biol. (IF 4.7) Pub Date : 2024-02-09 Anja Armbruster, Anna K. Ehret, Marissa Russ, Vincent Idstein, Melissa Klenzendorf, Denise Gaspar, Claudia Juraske, O. Sascha Yousefi, Wolfgang W. Schamel, Wilfried Weber, Maximilian Hörner
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Phenotypic Patterning through Copy Number Adaptation to Environmental Gradients ACS Synth. Biol. (IF 4.7) Pub Date : 2024-02-08 Paige Steppe, Camilo Rey-Bedón, Shalni Kumar, Emerald Forrest, Niklas Van Der Wagt, Arnav Tayal, Lev Tsimring, Jeff Hasty
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Biofoundry-Scale DNA Assembly Validation Using Cost-Effective High-Throughput Long-Read Sequencing ACS Synth. Biol. (IF 4.7) Pub Date : 2024-02-08 Peter Vegh, Sophie Donovan, Susan Rosser, Giovanni Stracquadanio, Rennos Fragkoudis
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Progress in Gene Editing and Metabolic Regulation of Saccharomyces cerevisiae with CRISPR/Cas9 Tools ACS Synth. Biol. (IF 4.7) Pub Date : 2024-02-07 Yaokun Liang, Song Gao, Xianghui Qi, Leonid N. Valentovich, Yingfeng An
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Transcription Factor Pdr3p Promotes Carotenoid Biosynthesis by Activating GAL Promoters in Saccharomyces cerevisiae ACS Synth. Biol. (IF 4.7) Pub Date : 2024-02-07 Ming-Kai Chen, Tang-Lei Zhang, Ming-Ze Sun, Hong-Wei Yu, Li-Dan Ye
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Development of Cell-Free Transcription–Translation Systems in Three Soil Pseudomonads ACS Synth. Biol. (IF 4.7) Pub Date : 2024-02-06 Joseph T. Meyerowitz, Elin M. Larsson, Richard M. Murray
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Exploration of the Native Sucrose Operon Enables the Development of an Inducible T7 Expression System in Paenibacillus polymyxa ACS Synth. Biol. (IF 4.7) Pub Date : 2024-02-06 Zilong Wang, Zhongpeng Guo, Yu Xin, Zhenghua Gu, Yi Shi, Ting Yang, Youran Li, Guiyang Shi, Zhongyang Ding, Liang Zhang
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Degron-Controlled Protein Degradation in Escherichia coli: New Approaches and Parameters ACS Synth. Biol. (IF 4.7) Pub Date : 2024-02-05 Glen E. Cronan, Andrei Kuzminov
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The SARS-CoV-2 Mpro Dimer-Based Screening System: A Synthetic Biology Tool for Identifying Compounds with Dimerization Inhibitory Potential ACS Synth. Biol. (IF 4.7) Pub Date : 2024-02-05 Ernawati Arifin Giri-Rachman, Vergio V. Effendy, Muhammad H. S. Azmi, Nicholas Yamahoki, Rebecca Stephanie, Dian F. Agustiyanti, Popi H. Wisnuwardhani, Marissa Angelina, Yana Rubiyana, Reza Aditama, Ratih A. Ningrum, Andri Wardiana, Azzania Fibriani
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Charge-Patterned Disordered Peptides Tune Intracellular Phase Separation in Bacteria ACS Synth. Biol. (IF 4.7) Pub Date : 2024-02-03 Jane Liao, Vivian Yeong, Allie C. Obermeyer
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DNA Logic Gates for Small Molecule Activation Circuits in Cells ACS Synth. Biol. (IF 4.7) Pub Date : 2024-02-02 Cole Emanuelson, Anirban Bardhan, Alexander Deiters
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Applications of CRISPR Epigenome Editors in Tumor Immunology and Autoimmunity ACS Synth. Biol. (IF 4.7) Pub Date : 2024-01-31 Berkay Yahsi, Fahreddin Palaz, Pervin Dincer
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Optimization of RNA Pepper Sensors for the Detection of Arbitrary RNA Targets ACS Synth. Biol. (IF 4.7) Pub Date : 2024-01-31 Anli A. Tang, Anna Afasizheva, Clara T. Cano, Kathrin Plath, Douglas Black, Elisa Franco
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DuBA.flow─A Low-Cost, Long-Read Amplicon Sequencing Workflow for the Validation of Synthetic DNA Constructs ACS Synth. Biol. (IF 4.7) Pub Date : 2024-01-31 Adán A. Ramírez Rojas, Cedric K. Brinkmann, Tania S. Köbel, Daniel Schindler
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MetJ-Based Mutually Interfering SAM-ON/SAM-OFF Biosensors ACS Synth. Biol. (IF 4.7) Pub Date : 2024-01-29 Taro Watanabe, Yuki Kimura, Daisuke Umeno
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DNA Reaction System That Acquires Classical Conditioning ACS Synth. Biol. (IF 4.7) Pub Date : 2024-01-27 Takashi Nakakuki, Masato Toyonari, Kaori Aso, Keiji Murayama, Hiroyuki Asanuma, Tom F. A. de Greef
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Engineering an Autonucleolytic Mammalian Suspension Host Cell Line to Reduce DNA Impurity Levels in Serum-Free Lentiviral Process Streams ACS Synth. Biol. (IF 4.7) Pub Date : 2024-01-24 Geoffrey Howe, Matthew Wasmuth, Pamela Emanuelle, Giulia Massaro, Ahad A. Rahim, Sadfer Ali, Milena Rivera, John Ward, Eli Keshavarz-Moore, Chris Mason, Darren N. Nesbeth
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Development of Lyophilized Eukaryotic Cell-Free Protein Expression System Based on Leishmania tarentolae ACS Synth. Biol. (IF 4.7) Pub Date : 2024-01-24 Juan Alfaro-Palma, Wayne A. Johnston, James Behrendorff, Zhenling Cui, Shayli Varasteh Moradi, Kirill Alexandrov
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Synthetic Cell Lines for Inducible Packaging of Influenza A Virus ACS Synth. Biol. (IF 4.7) Pub Date : 2024-01-23 Thu Phan, Qian Ye, Christopher Stach, Yu-Chieh Lin, Haoyu Cao, Annika Bowen, Ryan A. Langlois, Wei-Shou Hu
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Standardized Iterative Genome Editing Method for Escherichia coli Based on CRISPR-Cas9 ACS Synth. Biol. (IF 4.7) Pub Date : 2024-01-20 Huan Fang, Jianghua Zhao, Xinfang Zhao, Ning Dong, Ying Zhao, Dawei Zhang
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Genome-Wide CRISPR/Cas9 Screening Unveils a Novel Target ATF7IP–SETDB1 Complex for Enhancing Difficult-to-Express Protein Production ACS Synth. Biol. (IF 4.7) Pub Date : 2024-01-19 Su Hyun Kim, Jong-Ho Park, Sungwook Shin, Seunghyeon Shin, Dahyun Chun, Yeon-Gu Kim, Jiseon Yoo, Weon-Kyoo You, Jae Seong Lee, Gyun Min Lee
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pUdOs: Concise Plasmids for Bacterial and Mammalian Cells ACS Synth. Biol. (IF 4.7) Pub Date : 2024-01-18 France O. Manigat, Louise B. Connell, Brittany N. Stewart, Abdel-Rahman LePabic, Christian J. G. Tessier, Johnathon R. Emlaw, Nicholas D. Calvert, Anthony Rössl, Adam J. Shuhendler, Corrie J. B. daCosta, François-Xavier Campbell-Valois
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Correction to “DeCOIL: Optimization of Degenerate Codon Libraries for Machine Learning-Assisted Protein Engineering” ACS Synth. Biol. (IF 4.7) Pub Date : 2024-01-17 Jason Yang, Julie Ducharme, Kadina E. Johnston, Francesca-Zhoufan Li, Yisong Yue, Frances H. Arnold
Introduction, third paragraph, replace “Other studies take different approaches to bias libraries toward distributions of favorable variants, ranging from theoretical to more applied.16,51–55 Each study approaches the problem from a different angle, but none have been made both suitable and practical for the possible use cases encountered during MLPE (Figure 1A)” with “Other studies take different
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Novel Signal Peptides and Episomal Plasmid System for Enhanced Protein Secretion in Engineered Bacteroides Species ACS Synth. Biol. (IF 4.7) Pub Date : 2024-01-15 Tae Hyun Kim, Kowoon Ju, Seong Keun Kim, Seung-Gyun Woo, Jung-Sook Lee, Chul-Ho Lee, Eugene Rha, Jonghyeok Shin, Kil Koang Kwon, Hyewon Lee, Haseong Kim, Seung-Goo Lee, Dae-Hee Lee
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Special Issue on Artificial Intelligence for Synthetic Biology ACS Synth. Biol. (IF 4.7) Pub Date : 2024-01-12 Hector García Martín, Stanislav Mazurenko, Huimin Zhao