样式: 排序: IF: - GO 导出 标记为已读
-
Challenges Experienced and Observed during the Implementation of Leprosy Strategies, Sidama Region, Southern Ethiopia: An inductive thematic analysis of qualitative study among health professionals who working with leprosy programs. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-30 Kebede Tefera Betru,Thuledi Makua
BACKGROUND Prompt diagnosis and treatment of leprosy are crucial for preventing the disease's spread as well as for avoiding negative medical and social effects and reducing the disease's burden. The likelihood of nerve damage and subsequent disability rises as the length of the diagnostic delay. We aimed to explore the challenges of health professionals faced regarding their involvement in early leprosy
-
Variants of IL6, IL10, FCN2, RNASE3, IL12B and IL17B loci are associated with Schistosoma mansoni worm burden in the Albert Nile region of Uganda. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-30 Oscar Asanya Nyangiri,Julius Mulindwa,Joyce Namulondo,Anna Kitibwa,Jacent Nassuuna,Alison Elliott,Magambo Phillip Kimuda,Alex Boobo,Barbara Nerima,Moses Adriko,Nathan J Dunton,Gaganjit Kaur Madhan,Mark Kristiansen,Miriam Casacuberta-Partal,Harry Noyes,Enock Matovu,
BACKGROUND Individuals genetically susceptible to high schistosomiasis worm burden may contribute disproportionately to transmission and could be prioritized for control. Identifying genes involved may guide development of therapy. METHODOLOGY/PRINCIPAL FINDINGS A cohort of 606 children aged 10-15 years were recruited in the Albert Nile region of Uganda and assessed for Schistosoma mansoni worm burden
-
Large-scale releases and establishment of wMel Wolbachia in Aedes aegypti mosquitoes throughout the Cities of Bello, Medellín and Itagüí, Colombia. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-30 Iván Darío Velez,Alexander Uribe,Jovany Barajas,Sandra Uribe,Sandra Ángel,Juan David Suaza-Vasco,Maria Camila Mejia Torres,María Patricia Arbeláez,Eduardo Santacruz-Sanmartin,Lorena Duque,Luis Martínez,Tania Posada,Ana Cristina Patiño,Sandra Milena Gonzalez,Ana Lucía Velez,Jennifer Ramírez,Marlene Salazar,Sandra Gómez,Jorge E Osorio,Inaki Iturbe-Ormaetxe,Yi Dong,Frederico C Muzzi,Edwige Rances,Petrina
BACKGROUND The wMel strain of Wolbachia has been successfully introduced into Aedes aegypti mosquitoes and has been shown to reduce the transmission of dengue and other Aedes-borne viruses. Here we report the entomological results from phased, large-scale releases of Wolbachia infected Ae. aegypti mosquitoes throughout three contiguous cities located in the Aburrá Valley, Colombia. METHODOLOGY/PRINCIPAL
-
Reduced dengue incidence following city-wide wMel Wolbachia mosquito releases throughout three Colombian cities: Interrupted time series analysis and a prospective case-control study. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-30 Ivan Dario Velez,Stephanie K Tanamas,Maria Patricia Arbelaez,Simon C Kutcher,Sandra L Duque,Alexander Uribe,Lina Zuluaga,Luis Martínez,Ana Cristina Patiño,Jovany Barajas,Estefanía Muñoz,Maria Camila Mejia Torres,Sandra Uribe,Sandra Porras,Rita Almanza,Henry Pulido,Scott L O'Neill,Eduardo Santacruz-Sanmartin,Sandra Gonzalez,Peter A Ryan,Jai A Denton,Nicholas P Jewell,Suzanne M Dufault,Cameron P Simmons
BACKGROUND The introduction of Wolbachia (wMel strain) into Aedes aegypti mosquitoes reduces their capacity to transmit dengue and other arboviruses. Randomised and non-randomised studies in multiple countries have shown significant reductions in dengue incidence following field releases of wMel-infected Ae. aegypti. We report the public health outcomes from phased, large-scale releases of wMel-Ae
-
Morphological and functional cardiac alterations in children with congenital Zika syndrome and severe neurological deficits. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-29 Imara Correia de Queiroz Barbosa,Luizabel de Paula Gomes,Israel Nilton de Almeida Feitosa,Luís Fábio Barbosa Botelho,Bruno Robalinho Cavalcanti Barbosa,Alex Barbosa,André Telis de Vilela Araújo,Marcelo Dantas Tavares de Melo,Adriana Suely de Oliveira Melo,Vera Maria Cury Salemi
INTRODUCTION Zika virus infection during pregnancy causes fetal microcephaly and brain damage. Congenital Zika syndrome (CZS) is characterized by systemic involvement with diffuse muscle impairment, a high frequency of arthrogryposis, and microphthalmia. Cardiac impairment in CZS has rarely been evaluated. Our study assessed morphology and biventricular cardiac function in children with CZS and advanced
-
Vaccination against the HDL receptor of S. japonicum inhibits egg embryonation and prevents fatal hepatic complication in rabbit model. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-29 Jifeng Zhu,Lina Zhang,Zechao Xue,Zilüe Li,Chun Wang,Fanyan Chen,Yalin Li,Yang Dai,Yonghua Zhou,Sha Zhou,Xiaojun Chen,Kuniko Okumura-Noji,Rui Lu,Shinji Yokoyama,Chuan Su
BACKGROUND Schistosomiasis is one of the most important neglected tropical infectious diseases to overcome and the primary cause of its pathogenesis is ectopic maturation of the parasite eggs. Uptake of cholesteryl ester from the host high-density lipoprotein (HDL) is a key in this process in Schistosoma japonicum and CD36-related protein (CD36RP) has been identified as the receptor for this reaction
-
Diagnostic accuracy of qPCR and microscopy for cutaneous leishmaniasis in rural Ecuador: A Bayesian latent class analysis. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-29 Jacob M Bezemer,Joanna Merckx,Byron P Freire Paspuel,Manuel Calvopiña,Henry J C de Vries,Henk D F H Schallig,Mariska M G Leeflang,Nandini Dendukuri
BACKGROUND Clinical and laboratory diagnosis of cutaneous leishmaniasis (CL) is hampered by under-ascertainment of direct microscopy. METHODS This study compared the diagnostic accuracy of qPCR on DNA extracted from filter paper to the accuracy of direct smear slide microscopy in participants presenting with a cutaneous lesion suspected of leishmaniasis to 16 rural healthcare centers in the Ecuadorian
-
Mitochondrial protein BNIP3 regulates Chikungunya virus replication in the early stages of infection. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-27 Liliana Echavarria-Consuegra,Nilima Dinesh Kumar,Marleen van der Laan,Mario Mauthe,Denise Van de Pol,Fulvio Reggiori,Jolanda M Smit
Chikungunya virus (CHIKV) is a human pathogen causing outbreaks of febrile illness for which vaccines and specific treatments remain unavailable. Autophagy-related (ATG) proteins and autophagy receptors are a set of host factors that participate in autophagy, but have also shown to function in other unrelated cellular pathways. Although autophagy is reported to both inhibit and enhance CHIKV replication
-
Central nervous system infection in a pediatric population in West Java. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-27 Dewi H Alisjahbana,Syndi Nurmawati,Mia Milanti,Hofiya Djauhari,Jeremy P Ledermann,Ungke Antonjaya,Yora Permata Dewi,Edison Johar,Ageng Wiyatno,Ida Yus Sriyani,Bachti Alisjahbana,Dodi Safari,Khin Saw Aye Myint,Ann M Powers,Dzulfikar Dl Hakim
Central nervous system (CNS) viral infections are critical causes of morbidity and mortality in children; however, comprehensive data on etiology is lacking in developing countries such as Indonesia. To study the etiology of CNS infections in a pediatric population, 50 children admitted to two hospitals in Bandung, West Java, during 2017-2018 were enrolled in a CNS infection study. Cerebrospinal fluid
-
Epetraborole, a leucyl-tRNA synthetase inhibitor, demonstrates murine efficacy, enhancing the in vivo activity of ceftazidime against Burkholderia pseudomallei, the causative agent of melioidosis. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-27 Jason E Cummings,Christopher S Lunde,M R K Alley,Richard A Slayden
Burkholderia pseudomallei is the causative agent of melioidosis, which is increasingly being reported worldwide. Mortality rates as high as 40% have been reported based on clinical patient outcomes in the endemic areas of Australia and Thailand. Novel therapies are needed to reduce treatment duration and adverse effects and improve treatment outcomes. Epetraborole, a novel antibiotic, targets leucyl-tRNA
-
Impact of a small-scale tsetse fly control operation with deltamethrin impregnated "Tiny Targets" on tsetse density and trypanosomes' circulation in the Campo sleeping sickness focus of South Cameroon. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-27 Tito Tresor Melachio Tanekou,Calmes Ursain Bouaka Tsakeng,Inaki Tirados,Alphonse Acho,Jude Bigoga,Charles Sinclair Wondji,Flobert Njiokou
BACKGROUND Significant progress has been made towards African sleeping sickness elimination in the last decade. Indeed, the World Health Organization (WHO) global goal of eliminating the chronic form of the disease as a public health problem was achieved in 2020 (i.e., < 2,000 new cases per year). Vector control has played an important role in achieving this goal. In this study, we evaluated the impact
-
Effects of human mobility on the spread of Dengue in the region of Caldas, Colombia. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-27 Carolina Ospina-Aguirre,David Soriano-Paños,Gerard Olivar-Tost,Cristian C Galindo-González,Jesús Gómez-Gardeñes,Gustavo Osorio
According to the World Health Organization (WHO), dengue is the most common acute arthropod-borne viral infection in the world. The spread of dengue and other infectious diseases is closely related to human activity and mobility. In this paper we analyze the effect of introducing mobility restrictions as a public health policy on the total number of dengue cases within a population. To perform the
-
Minimal effective dose of bedaquiline administered orally and activity of a long acting formulation of bedaquiline in the murine model of leprosy. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-27 Aurélie Chauffour,Nacer Lounis,Koen Andries,Vincent Jarlier,Nicolas Veziris,Alexandra Aubry
BACKGROUND Bedaquiline (BDQ), by targeting the electron transport chain and having a long half-life, is a good candidate to simplify leprosy treatment. Our objectives were to (i) determine the minimal effective dose (MED) of BDQ administered orally, (ii) evaluate the benefit of combining two inhibitors of the respiratory chain, BDQ administered orally and clofazimine (CFZ)) and (iii) evaluate the benefit
-
An evaluation of Mp1p antigen screening for talaromycosis in HIV-infected antiretroviral therapy-naïve population in Guangdong, China. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-27 Dandan Gong,Weiyin Lin,Huihua Zhang,Xu Ou,Liya Li,Pengle Guo,Yaozu He,Cong Liu,Weiping Cai,Xiaoping Tang,Linghua Li
BACKGROUND Talaromycosis is one of the most common opportunistic infections in human immunodeficiency virus (HIV) infected patients. However, few researches have explored the prevalence in Southern China and fully assessed the value of the Mp1p antigen screening for the diagnosis of talaromycosis. METHODOLOGY/PRINCIPAL FINDINGS We performed a cross-sectional study of HIV-infected antiretroviral therapy
-
Bothrops venom-induced hemostasis disorders in the rat: Between Scylla and Charybdis. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-27 Sébastien Larréché,Lucie Chevillard,Georges Jourdi,Simon Mathé,Aurélie Servonnet,Bérangère S Joly,Virginie Siguret,Jean-Philippe Chippaux,Bruno Mégarbane
Hemostasis impairment represents the most threatening consequence of Viperidae envenoming, notably with Bothrops genus. In the French departments of America, B. atrox envenomation in French Guiana may lead to bleeding while B. lanceolatus envenomation in Martinique to thrombosis. Bleeding related to B. atrox envenomation is attributed to vascular damage mediated by venom metalloproteinases and blood
-
The economic burden of Chagas disease: A systematic review. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-22 Mônica Viegas Andrade,Kenya Valéria Micaela de Souza Noronha,Aline de Souza,André Soares Motta-Santos,Paulo Estevão Franco Braga,Henrique Bracarense,Maria Carolina Corrêa de Miranda,Bruno Ramos Nascimento,Israel Molina,Francisco Rogerlândio Martins-Melo,Pablo Perel,Yvonne Geissbühler,Monica Quijano,Isis Eloah Machado,Antônio Luiz Pinho Ribeiro
BACKGROUND Chagas disease (CD) is a neglected disease affecting millions worldwide, yet little is known about its economic burden. This systematic review is part of RAISE project, a broader study that aims to estimate the global prevalence, mortality, and health and economic burden attributable to chronic CD and Chronic Chagas cardiomyopathy. The objective of this study was to assess the main costs
-
Safety and efficacy of paromomycin/miltefosine/liposomal amphotericin B combinations for the treatment of post-kala-azar dermal leishmaniasis in Sudan: A phase II, open label, randomized, parallel arm study. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-21 Brima Musa Younis,Ahmed Mudawi Musa,Séverine Monnerat,Mohammed Abdelrahim Saeed,Eltahir Awad Gasim Khalil,Anas Elbashir Ahmed,Mujahid Ahmed Ali,Ali Noureldin,Gina Muthoni Ouattara,Godfrey M Nyakaya,Samuel Teshome,Truphosa Omollo,Michael Ochieng,Thaddaeus Egondi,Mildred Mmbone,Wan-Yu Chu,Thomas P C Dorlo,Eduard E Zijlstra,Monique Wasunna,Jorge Alvar,Fabiana Alves
BACKGROUND Treatment for post-kala-azar dermal leishmaniasis (PKDL) in Sudan is currently recommended only for patients with persistent or severe disease, mainly because of the limitations of current therapies, namely toxicity and long hospitalization. We assessed the safety and efficacy of miltefosine combined with paromomycin and liposomal amphotericin B (LAmB) for the treatment of PKDL in Sudan
-
Health professionals' knowledge on dengue and health facility preparedness for case detection: A cross-sectional study in Dar es Salaam, Tanzania. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-21 Ummul-Khair Mustafa,Elingarami Sauli,Johanna Brinkel,Katharina Sophia Kreppel
Dengue presents a growing public health concern in the Dar es Salaam region of Tanzania, marked by the recurring incidence of outbreaks. Unfortunately, there is little information available on the region's preparedness in terms of health care workers' knowledge on dengue as well as the availability of reagents and equipment essential for diagnosing and monitoring of dengue infections. To elucidate
-
Impaired functions of human monocyte-derived dendritic cells and induction of regulatory T cells by pathogenic Leptospira. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-20 Pratomporn Krangvichian,Teerasit Techawiwattanaboon,Tanapat Palaga,Patcharee Ritprajak,Patipark Kueanjinda,Chamraj Kaewraemruaen,Kanitha Patarakul
Leptospirosis is a global zoonosis caused by pathogenic Leptospira. The disease outcome is influenced by the interplay between innate and adaptive immune responses. Dendritic cells (DCs) play a crucial role in shaping the adaptive immune response. A recent study revealed that pathogenic Leptospira limited the activation of human monocyte-derived dendritic cells (MoDCs) compared to non-pathogenic Leptospira
-
"I sold my towel and shoes to pay the traditional healer": Care-seeking costs and productivity losses among snakebite victims in Eastern Province, Rwanda. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-20 Janna M Schurer,Mahlet Tadesse Admasu,Mihigo Bonaventure,Dieudonne Hakizimana,Elijah Murara,Lauren E MacDonald,Ellen Rafferty
Snakebite envenomation (SBE) is endemic to sub-Saharan Africa and generally over-represented in rural, remote, and impoverished agricultural communities. While poverty is an established risk factor, little research has been done to investigate the economic consequences of SBE. This cross-sectional, quantitative study aimed to measure out-of-pocket spending and lost income when a household member was
-
Is there still yaws in Nigeria? Active case search in endemic areas of southern Nigeria. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-20 Ngozi Ekeke,Francis S Iyama,Joseph N Chukwu,Kingsley Asiedu,Michael Marks,Babatunde Omotowo,Olanike Agwu-Umahi,Victor O Nvene,Shiloh Paul,Charles C Nwafor,Anthony O Meka,Chinwe C Eze,Okechukwu E Ezeakile,Martin I Njoku,Ngozi N Murphy-Okpala
BACKGROUND Yaws is a disease caused by the bacteria Treponema pallidum subspecies pertenue, which is most commonly seen among children below 15 years. In the twentieth century yaws was endemic in Nigeria but eradication strategies markedly reduced the disease burden. Currently there is minimal data on the ongoing transmission of yaws in Nigeria, despite reports of confirmed yaws cases in neighbouring
-
Epidemiology of giardiasis and assemblages A and B and effects on diarrhea and growth trajectories during the first 8 years of life: Analysis of a birth cohort in a rural district in tropical Ecuador. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-20 Tannya Sandoval-Ramírez,Victor Seco-Hidalgo,Evelyn Calderon-Espinosa,Diana Garcia-Ramon,Andrea Lopez,Manuel Calvopiña,Irene Guadalupe,Martha Chico,Rojelio Mejia,Irina Chis Ster,Philip J Cooper
BACKGROUND There are limited longitudinal data on the acquisition of Giardia lamblia infections in childhood using molecular assays to detect and type assemblages, and measure effects of infections on diarrhea risk and childhood growth. METHODS We analysed stool samples from a surveillance sample within a birth cohort in a rural district in tropical Ecuador. The cohort was followed to 8 years of age
-
EDIII-Fc induces protective immune responses against the Zika virus in mice and rhesus macaque. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-20 Hailong Su,Jun Liu,Jianhai Yu,Zhenzhen Qiu,Wenhan Liang,Wangsheng Wu,Haifeng Mo,Hongwei Li,Wei Zhao,Weiwang Gu
Zika virus can infect the fetus through the placental barrier, causing ZIKV congenital syndrome and even miscarriage, which can cause great harm to pregnant women and infants. Currently, there is no vaccine and drug available to combat the Zika virus. In this study, we designed a fusion protein named EDIII-Fc, including the EDIII region of Zika E protein and human IgG Fc fragment, and obtained 293T
-
Mayaro virus pathogenesis and immunity in rhesus macaques. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-20 Whitney C Weber,Caralyn S Labriola,Craig N Kreklywich,Karina Ray,Nicole N Haese,Takeshi F Andoh,Michael Denton,Samuel Medica,Magdalene M Streblow,Patricia P Smith,Nobuyo Mizuno,Nina Frias,Miranda B Fisher,Aaron M Barber-Axthelm,Kimberly Chun,Samantha Uttke,Danika Whitcomb,Victor DeFilippis,Shauna Rakshe,Suzanne S Fei,Michael K Axthelm,Jeremy V Smedley,Daniel N Streblow
Mayaro virus (MAYV) is a mosquito-transmitted alphavirus that causes debilitating and persistent arthritogenic disease. While MAYV was previously reported to infect non-human primates (NHP), characterization of MAYV pathogenesis is currently lacking. Therefore, in this study we characterized MAYV infection and immunity in rhesus macaques. To inform the selection of a viral strain for NHP experiments
-
Inverted U-shaped association between bacillary dysentery and temperature: A new finding using a novel two-stage strategy in multi-region studies. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-17 Wei Wang,Yunqiong Wang,Lin Chen,Bo Zhou,Fang Liao
BACKGROUND Bacillary dysentery (BD) has brought a significant public health concern in China. Temperature is one of the main factors affecting BD incidence. Due to the largely different temperature ranges between regions, the classic multi-region time series studies could only explore the relative temperature-BD association and showed that BD incidence is positively associated with relative temperature
-
Vegetable oil-based surfactants are adjuvants that enhance the efficacy of neonicotinoid insecticides and can bias susceptibility testing in adult mosquitoes. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-17 Fred A Ashu,Caroline Fouet,Marilene M Ambadiang,Véronique Penlap-Beng,Colince Kamdem
BACKGROUND The standard operating procedure for testing the susceptibility of adult mosquitoes to neonicotinoid or butenolide insecticides recommends using a vegetable oil ester (Mero) as a surfactant. However, there is growing evidence that this adjuvant contains surfactants that can enhance insecticide activity, mask resistance and bias the bioassay. METHODOLOGY/PRINCIPAL FINDINGS Using standard
-
Remote evaluation of STH program coverage: Experiences from the DeWorm3 study, India. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-16 Kumudha Aruldas,Rohan Michael Ramesh,William E Oswald,Venkateshprabhu Janagaraj,Angelin Titus,Jabaselvi Johnson,Malvika Saxena,Gideon John Israel,Katherine Halliday,Judd L Walson,Arianna Rubin Means,Sitara Swarna Rao Ajjampur
BACKGROUND The DeWorm3 trial is a multi-country study testing the feasibility of interrupting transmission of soil-transmitted helminths by community-wide mass drug administration (cMDA). Treatment coverage during cMDA delivery was validated by in-person coverage evaluation surveys (CES) after each round of treatment. A mobile phone-based CES was carried out in India when access to households was restricted
-
Progress towards elimination of onchocerciasis transmission in Mali: A "pre-stop MDA" survey in 18 transmission zones. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-15 Housseini Dolo,Michel Emmanuel Coulibaly,Moussa Sow,Yaya Ibrahim Coulibaly,Mama Doumbia,Moussa Sangare,Abdoul Sanogo,Benoit Dembele,Boubacar Guindo,Mamadou Coulibaly,Modibo Keita,Lamine Soumaoro,Dansine Diarra,Boubacar Dicko,Louise Hamill,Seydou Doumbia,Hamadoun Sangho,Yacouba Sangare,Yaobi Zhang,Jamie Tallant,Alpha Seydou Yaro,Charles Mackenzie,Thomas B Nutman,Daniel Boakye
BACKGROUND Onchocerciasis control activities in Mali began in 1975 with vector larviciding carried out by the Onchocerciasis Control Programme (OCP), followed by the distribution of ivermectin from 1998 until the closure of the OCP in 2002. At that time, epidemiological evaluations, using skin snip microscopy and O-150 pool screening PCR in black flies, indicated that the disease had been largely controlled
-
Socioeconomic, meteorological factors and spatiotemporal distribution of human brucellosis in China between 2004 and 2019-A study based on spatial panel model. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-13 Zi-Xin Sun,Yan Wang,Ying-Jie Li,Shi-Hao Yu,Wei Wu,De-Sheng Huang,Peng Guan
BACKGROUND Human brucellosis continues to be a great threat to human health in China. The present study aimed to investigate the spatiotemporal distribution of human brucellosis in China from 2004 to 2019, to analyze the socioeconomic factors, meteorological factors and seasonal effect affecting human brucellosis incidence in different geographical regions with the help of spatial panel model, and
-
Wide reference databases for typing Trypanosoma cruzi based on amplicon sequencing of the minicircle hypervariable region. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-13 Fanny Rusman,Anahí G Díaz,Tatiana Ponce,Noelia Floridia-Yapur,Christian Barnabé,Patricio Diosque,Nicolás Tomasini
BACKGROUND Trypanosoma cruzi, the etiological agent of Chagas Disease, exhibits remarkable genetic diversity and is classified into different Discrete Typing Units (DTUs). Strain typing techniques are crucial for studying T. cruzi, because their DTUs have significant biological differences from one another. However, there is currently no methodological strategy for the direct typing of biological materials
-
Near-infrared spectroscopy and machine learning algorithms for rapid and non-invasive detection of Trichuris. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-13 Tharanga N Kariyawasam,Silvia Ciocchetta,Paul Visendi,Ricardo J Soares Magalhães,Maxine E Smith,Paul R Giacomin,Maggy T Sikulu-Lord
BACKGROUND Trichuris trichiura (whipworm) is one of the most prevalent soil transmitted helminths (STH) affecting 604-795 million people worldwide. Diagnostic tools that are affordable and rapid are required for detecting STH. Here, we assessed the performance of the near-infrared spectroscopy (NIRS) technique coupled with machine learning algorithms to detect Trichuris muris in faecal, blood, serum
-
Modelling seasonality of Lassa fever incidences and vector dynamics in Nigeria. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-13 James Q McKendrick,Warren S D Tennant,Michael J Tildesley
Lassa fever (Lf) is a viral haemorrhagic disease endemic to West Africa and is caused by the Lassa mammarenavirus. The rodent Mastomys natalensis serves as the primary reservoir and its ecology and behaviour have been linked to the distinct spatial and temporal patterns in the incidence of Lf. Nigeria has experienced an unprecedented epidemic that lasted from January until April of 2018, which has
-
Fungal mycetoma and pregnancy: An association with costly and difficult management, about a case. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-13 Niare Ndour,Mame Tené Ndiaye,Assane Diop,Ramatoulaye Ly,Coumba Ndiaye,Mamadou Sarr,Astou Diouf,Fatou Diagne,Fatimata Ly
Mycetomas are endemic diseases in tropical and sub-tropical countries of Africa, Asia and America, mainly affecting rural populations living below the poverty line. We report a particular case of a mycetoma associated with pregnancy whose evolution was good, but at the cost of significant financial expenses. This was a 39-year-old woman who developed a fungal mycetoma due to Madurella mycetomatis from
-
Public-private partnerships influencing the initiation and duration of clinical trials for neglected tropical diseases. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-13 Zhongxuan Ma,Kevin Augustijn,Iwan De Esch,Bart Bossink
Public-private partnerships (PPPs) for neglected tropical diseases (NTDs) are often studied as an organizational form that facilitates the management and control of the huge costs of drug research and development. Especially the later stages of drug development, including clinical trials, become very expensive. This present study investigates whether and how the type of PPPs influences the initiation
-
ASPSCR-1 and Sirt-5 alleviate Clonorchis liver fluke rCsNOSIP-induced oxidative stress, proliferation, and migration in cholangiocarcinoma cells. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-10 Meng Bian,Shan Li,Hanzong Zhou,Lijun Bi,Yong Shen,Chen Tingjin,Xinbing Yu,Yan Huang,Qingxia Xu
BACKGROUND Clonorchiasis, caused by the infection of Clonorchis sinensis (C. sinensis), is a kind of neglected tropical disease, but it is highly related to cholangiocarcinoma. It has been well known that NO from chronic inflammation responses are thought to be a major component of the damage and ultimate carcinogenesis ESPs such as nitric oxide synthase interacting protein (NOSIP) are thought to enhance
-
Snakebite epidemiology, outcomes and multi-cluster risk modelling in Eswatini. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-10 Sara Padidar,Ara Monadjem,Thea Litschka-Koen,Brent Thomas,Nondusimo Shongwe,Clare Baker,Lindelwa Mmema,Trevor Sithole,James Murray,Nicholas R Casewell,Jonathan Pons,David G Lalloo,Robert A Harrison,Ymkje Stienstra,Wisdom M Dlamini
BACKGROUND Halving snakebite morbidity and mortality by 2030 requires countries to develop both prevention and treatment strategies. The paucity of data on the global incidence and severity of snakebite envenoming causes challenges in prioritizing and mobilising resources for snakebite prevention and treatment. In line with the World Health Organisation's 2019 Snakebite Strategy, this study sought
-
Gendered lives, gendered Vulnerabilities: An intersectional gender analysis of exposure to and treatment of schistosomiasis in Pakwach district, Uganda. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-10 Sarah N Ssali,Rosemary Morgan,Salama Nakiranda,Christopher K Opio,Mariam Otmani Del Barrio
INTRODUCTION Schistosomiasis is a neglected tropical disease (NTD) that is endemic in Uganda, despite several interventions to eliminate it. It is transmitted when people infected with it pass on their waste matter into fresh water bodies used by others, consequently infecting them. Several studies have demonstrated gender and age differences in prevalence of schistosomiasis and NTDs such as lymphatic
-
Glucose metabolism sustains heme-induced Trypanosoma cruzi epimastigote growth in vitro. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-10 Carolina Silva Dias Vieira,Ramon Pinheiro Aguiar,Natalia Pereira de Almeida Nogueira,Gilson Costa Dos Santos Junior,Marcia Cristina Paes
Chagas disease is caused by the protozoan parasite, Trypanosoma cruzi. This parasite alternates between an insect vector and a mammalian host. T. cruzi epimastigotes reside in the insect vector and coexist with the blood components of the vertebrate host. The metabolic profile of T. cruzi has been extensively studied; however, changes in its metabolism in response to signaling molecules present in
-
The surveillance of plague among rodents and dogs in Western Iran. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-10 Saber Esmaeili,Ahmad Mahmoudi,Parisa Esmaeili,Zohreh Yousefi Ghalejoogh,Alireza Mordadi,Ahmad Ghasemi,Ali Mohammadi,Amin Bagheri,Aria Sohrabi,Mina Latifian,Minoarisoa Rajerison,Javier Pizarro-Cerda,Ehsan Mostafavi
BACKGROUND The causative agent of plague, Yersinia pestis, is maintained in nature via a flea-rodent cycle. Western Iran is an old focus for plague, and recent data indicate that rodents and dogs in this region have serological evidence of Y. pestis infection. The purpose of this study was to conduct a large-scale investigation of Y. pestis infection in shepherd dogs, rodents, and their fleas in old
-
Prevalence and clinical characteristics of increased pancreatic enzymes in patients with severe fever with thrombocytopenia syndrome. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-09 Zhongwei Zhang,Xue Hu,Qunqun Jiang,Qian Du,Jie Liu,Mingqi Luo,Liping Deng,Yong Xiong
BACKGROUND AND AIM The increased pancreatic enzymes have recently been reported in patients with severe fever with thrombocytopenia syndrome (SFTS). However, its significance has not been elucidated clearly. The aim of this study was to explore the prevalence, clinical characteristics of elevated pancreatic enzymes (amylase and lipase) and its association with AP in patients with SFTS. METHODS Data
-
Influence of previous Zika virus infection on acute dengue episode. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-09 Cassia F Estofolete,Alice F Versiani,Fernanda S Dourado,Bruno H G A Milhim,Carolina C Pacca,Gislaine C D Silva,Nathalia Zini,Barbara F Dos Santos,Flora A Gandolfi,Natalia F B Mistrão,Pedro H C Garcia,Rodrigo S Rocha,Lee Gehrke,Irene Bosch,Rafael E Marques,Mauro M Teixeira,Flavio G da Fonseca,Nikos Vasilakis,Maurício L Nogueira
BACKGROUND The co-circulation of flaviviruses in tropical regions has led to the hypothesis that immunity generated by a previous dengue infection could promote severe disease outcomes in subsequent infections by heterologous serotypes. This study investigated the influence of antibodies generated by previous Zika infection on the clinical outcomes of dengue infection. METHODOLOGY/PRINCIPAL FINDINGS
-
Performance of diagnostic tests for Trypanosoma brucei brucei in experimentally infected pigs. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-09 Kadidiata Ilboudo,Alain Boulangé,Robert Eustache Hounyèmè,Geoffrey Gimonneau,Jacques Kaboré,Adrien Gaston Marie Belem,Marc Desquesnes,Veerle Lejon,Mathurin Koffi,Vincent Jamonneau,Sophie Thévenon
Animal African trypanosomosis is an important vector-borne disease of livestock in sub-Saharan Africa. Pigs seem relatively tolerant to trypanosome infection and could act as a reservoir of trypanosomes affecting animals and humans. Our ability to reliably detect trypanosome infection in pigs depends on the performance of diagnostic tools, which is not well known. In pigs experimentally infected with
-
Assessment of the impact of implementation research on the Visceral Leishmaniasis (VL) elimination efforts in Nepal. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-09 Anand Ballabh Joshi,Megha Raj Banjara,Sachi Chuke,Axel Kroeger,Saurabh Jain,Abraham Aseffa,John C Reeder
Nepal, Bangladesh, and India signed a Memorandum of Understanding (MoU) in 2005 to eliminate visceral leishmaniasis (VL) as a public health problem from the Indian subcontinent by 2015. By 2021, the number of reported VL cases in these countries had declined by over 95% compared to 2007. This dramatic success was achieved through an elimination programme that implemented early case detection and effective
-
Prevalence and morbidity of urogenital schistosomiasis among pre-school age children in Cubal, Angola. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-08 Raquel Sánchez-Marqués,Cristina Bocanegra,Fernando Salvador,Arlette Nindia,Zeferino Pintar,Joan Martínez-Campreciós,Sandra Aixut,Patricia Mossalilo,Elena Sulleiro,María Espiau,Santiago Mas-Coma,Ma Dolores Bargues,Israel Molina
BACKGROUND Schistosomiasis is one of the most important neglected tropical diseases, with a great impact on public health and more than 200,000 deaths annually. Schistosoma haematobium causes urinary tract (UT) morbidity. Since schistosomiasis morbidity control programs focus on children older than 5 years, pre-school age children (PSAC) morbidity is not well known. METHODS We conducted a cross-sectional
-
Molecular surveillance of insecticide resistance in Phlebotomus argentipes targeted by indoor residual spraying for visceral leishmaniasis elimination in India. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-08 Emma Reid,Rinki Michelle Deb,Asgar Ali,Rudra Pratap Singh,Prabhas Kumar Mishra,Josephine Shepherd,Anand Mohan Singh,Aakanksha Bharti,Chandramani Singh,Sadhana Sharma,Michael Coleman,David Weetman
Molecular surveillance of resistance is an increasingly important part of vector borne disease control programmes that utilise insecticides. The visceral leishmaniasis (VL) elimination programme in India uses indoor residual spraying (IRS) with the pyrethroid, alpha-cypermethrin to control Phlebotomus argentipes the vector of Leishmania donovani, the causative agent of VL. Prior long-term use of DDT
-
Dengue virus infection induces selective expansion of Vγ4 and Vγ6TCR γδ T cells in the small intestine and a cytokine storm driving vascular leakage in mice. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-08 Takeshi Kurosu,Daisuke Okuzaki,Yusuke Sakai,Mohamad Al Kadi,Supranee Phanthanawiboon,Yasusi Ami,Masayuki Shimojima,Tomoki Yoshikawa,Shuetsu Fukushi,Noriyo Nagata,Tadaki Suzuki,Daisuke Kamimura,Masaaki Murakami,Hideki Ebihara,Masayuki Saijo
Dengue is a major health problem in tropical and subtropical regions. Some patients develop a severe form of dengue, called dengue hemorrhagic fever, which can be fatal. Severe dengue is associated with a transient increase in vascular permeability. A cytokine storm is thought to be the cause of the vascular leakage. Although there are various research reports on the pathogenic mechanism, the complete
-
Advocating for PCR-RFLP as molecular tool within malaria programs in low endemic areas and low resource settings. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-08 Mergiory Y Labadie-Bracho,Malti R Adhin
The road to malaria elimination for low- and middle-income countries is paved with obstacles, including the complexity and high costs of advanced molecular methods for genomic analysis. The usefulness of PCR-RFLP as less complex and affordable molecular surveillance tool in low-endemic malaria regions was assessed in a cross-sectional study conducted in Suriname, currently striving for malaria elimination
-
Real life condition evaluation of Inoserp PAN-AFRICA antivenom effectiveness in Cameroon. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-08 Jean-Philippe Chippaux,Rodrigue Ntone,David Benhammou,Yoann Madec,Gaëlle Noël,Anais Perilhou,Fai Karl,Pierre Amta,Marie Sanchez,Lucrece Matchim,Pedro Clauteaux,Lucrèce Eteki,Mark Ndifon,Yap Boum,Armand S Nkwescheu,Fabien Taieb
BACKGROUND Snakebites is a serious public health issue but remains a neglected tropical disease. Data on antivenom effectiveness are urgently needed in Africa. We assessed effectiveness of Inoserp PAN-AFRICA (IPA), the recommended antivenom available in Cameroon. METHODOLOGY/PRINCIPAL FINDINGS We enrolled 447 patients presenting with snakebite in 14 health facilities across Cameroon. At presentation
-
Trends in antimicrobial resistance amongst Salmonella Paratyphi A isolates in Bangladesh: 1999-2021. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-08 Mohammad S I Sajib,Arif M Tanmoy,Yogesh Hooda,Hafizur Rahman,Sira J Munira,Anik Sarkar,Dipu Das,Md Asadur Rahman,Nazrul Islam,Mohammod Shahidullah,Md Ruhul Amin,Md Jahangir Alam,Mohammed Hanif,Stephen P Luby,Denise O Garrett,Samir K Saha,Senjuti Saha
BACKGROUND Typhoid and paratyphoid remain common bloodstream infections in areas with suboptimal water and sanitation infrastructure. Paratyphoid, caused by Salmonella Paratyphi A, is less prevalent than typhoid and its antimicrobial resistance (AMR) trends are less documented. Empirical treatment for paratyphoid is commonly based on the knowledge of susceptibility of Salmonella Typhi, which causes
-
Molecular subtypes of Adenovirus-associated acute respiratory infection outbreak in children in Northern Vietnam and risk factors of more severe cases. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-07 Dinh-Dung Nguyen,Lan Tuyet Phung,Huyen Thi Thanh Tran,Ha Thi Thanh Ly,Anh Hang Mai Vo,Nhung Phuong Dinh,Phuong Mai Doan,Anh Thi Nguyen,Luc Danh Dang,Thia Thi Doan,Khuong Thi Pham,Huong Lan Pham,Dai Xuan Hoang,Thao Ngoc Pham,Bao Thai Tran,Trang Thi Thuc Tran,Huong Thi Minh Le,An Nhat Pham,Antony Antoniou,Nhan Thi Ho
BACKGROUND Under the pressure of Human Adenovirus (HAdV)-associated acute respiratory infection (ARI) outbreak in children in Northern Vietnam in the end of 2022, this study was initiated to identify the HAdV subtype(s) and examine the associated clinical features and risk factors of more severe cases. METHODS This study evaluated pediatric patients with ARI which had tested positive for HAdV between
-
Anti-tick vaccine candidate subolesin is important for blood feeding and innate immune gene expression in soft ticks. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-07 Krittika Nandy,Comfort Tamakloe,Daniel E Sonenshine,Hameeda Sultana,Girish Neelakanta
Subolesin is a conserved molecule in both hard and soft ticks and is considered as an effective candidate molecule for the development of anti-tick vaccine. Previous studies have reported the role of subolesin in blood feeding, reproduction, development, and gene expression in hard ticks. However, studies addressing the role of subolesin in soft ticks are limited. In this study, we report that subolesin
-
Trachoma risk factors in Oromia Region, Ethiopia. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-07 Oumer Shafi Abdurahman,Anna Last,David Macleod,Esmael Habtamu,Bart Versteeg,Gebeyehu Dumessa,Meseret Guye,Rufia Nure,Dereje Adugna,Hirpha Miecha,Katie Greenland,Matthew J Burton
BACKGROUND Trachoma, the leading infectious cause of blindness, is caused by the bacterium Chlamydia trachomatis (Ct). Despite enormous disease control efforts and encouraging progress, trachoma remains a significant public health problem in 44 countries. Ethiopia has the greatest burden of trachoma worldwide, however, robust data exploring transmission risk factors and the association between socio-economic
-
Hepatic macrophages play critical roles in the establishment and growth of hydatid cysts in the liver during Echinococcus granulosus sensu stricto infection. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-06 Hui Wang,Qian Yu,Mingkun Wang,Jiao Hou,Maolin Wang,Xuejiao Kang,Xinling Hou,Dewei Li,Zibigu Rousu,Tiemin Jiang,Jing Li,Hao Wen,Chuanshan Zhang
Cystic echinococcosis (CE) is a worldwide neglected zoonotic disease caused by infection with the larval stage of the tapeworm Echinococcus granulosus sensu lato (E. granulosus s.l.), which predominantly resides in the liver accompanied by mild inflammation. Macrophages constitute the main cellular component of the liver and play a central role in controlling the progression of inflammation and liver
-
Field validation of different intervention modes for the potential transmission risk of schistosomiasis during post-transmission interruption phase. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-06 Jiaxin Feng,Zhaoyu Guo,Peijun Qian,Wenya Wang,Hehua Hu,Xia Zhang,JingBo Xue,Yinlong Li,Chunli Cao,Yuwan Hao,Shizhu Li
OBJECTIVE Precision interventions have been proposed in transmission-interrupted areas to further reduce the potential transmission risk of schistosomiasis. This study aimed to evaluate the effects of different interventions modes for potential transmission risk control. METHODS Three groups of schistosomiasis-endemic villages were selected in Jiangling county, Hubei province. After baseline surveys
-
Distribution and treatment needs of soil-transmitted helminthiasis in Bangladesh: A Bayesian geostatistical analysis of 2017-2020 national survey data. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-06 Daniel J F Gerber,Sanjaya Dhakal,Md Nazmul Islam,Abdullah Al Kawsar,Md Abul Khair,Md Mujibur Rahman,Md Jahirul Karim,Md Shafiqur Rahman,M M Aktaruzzaman,Cara Tupps,Mariana Stephens,Paul M Emerson,Jürg Utzinger,Penelope Vounatsou
BACKGROUND In Bangladesh, preventive chemotherapy targeting soil-transmitted helminth (STH) infections in school-age children has been implemented since 2008. To evaluate the success of this strategy, surveys were conducted between 2017 and 2020 in 10 out of 64 districts. We estimate the geographic distribution of STH infections by species at high spatial resolution, identify risk factors, and estimate
-
Phylogenomics of Leptospira santarosai, a prevalent pathogenic species in the Americas. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-02 Diana Chinchilla,Cecilia Nieves,Ricardo Gutiérrez,Vallier Sordoillet,Frédéric J Veyrier,Mathieu Picardeau
BACKGROUND Leptospirosis is a complex zoonotic disease mostly caused by a group of eight pathogenic species (L. interrogans, L. borgpetersenii, L. kirschneri, L. mayottensis, L. noguchii, L. santarosai, L. weilii, L. alexanderi), with a wide spectrum of animal reservoirs and patient outcomes. Leptospira interrogans is considered as the leading causative agent of leptospirosis worldwide and it is the
-
Detection of coinfection with Primate Erythroparvovirus 1 and arboviruses (DENV, CHIKV and ZIKV) in individuals with acute febrile illness in the state of Rio Grande do Norte in 2016. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-02 Vanessa Dos Santos Morais,Lídia Maria Reis Santana,João Felipe Bezerra,Flavia Emmanuelle Cruz,Themis Rocha de Souza,Roozbeh Tahmasebi,Rafael Augusto Alves Raposo,Roberta Marcatti,Erick Matheus Garcia Barbosa,Philip Michael Hefford,Renata Buccheri,Ester Cerdeira Sabino,Antonio Charlys da Costa
BACKGROUND Arthropod-borne viruses, known as arboviruses, pose substantial risks to global public health. Dengue (DENV), Chikungunya (CHIKV) and Zika (ZIKV) viruses stand out as significant concerns in Brazil and worldwide. Their overlapping clinical manifestations make accurate diagnosis a challenge, underscoring the need for reliable laboratory support. This study employs a comprehensive molecular
-
The responsibility of thwarting and managing Japanese encephalitis cannot be understated-Outbreaks or resurgences may manifest, catching us off guard. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-02 Ran Wang,Zhengde Xie
-
Fitness costs in the presence and absence of insecticide use explains abundance of two common Aedes aegypti kdr resistance alleles found in the Americas. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-01 Juan J Silva,Cera R Fisher,Anastacia E Dressel,Jeffrey G Scott
Aedes aegypti is the vector of viruses such as chikungunya, dengue, yellow fever and Zika that have a critical impact on human health. Control of adult mosquitoes is widely done using pyrethroids, but resistance has reduced the effectiveness of this class of insecticides. Resistance to pyrethroids in mosquitoes is commonly due to mutations in the voltage-gated sodium channel (Vgsc) gene (these mutations
-
Tumor necrosis factor-α (TNF-α) -308G >a promoter polymorphism (rs1800629) promotes Asians in susceptibility to Plasmodium falciparum severe malaria: A meta-analysis. PLOS Negl. Trop. Dis. (IF 3.8) Pub Date : 2023-11-01 Panida Kongjam,Noel Pabalan,Phuntila Tharabenjasin,Hamdi Jarjanazi,Wanna Chaijaroenkul,Kesara Na-Bangchang
The multifactorial pathogenesis of severe malaria is partly attributed to host genes, such as those encoding cytokines involved in complex inflammatory reactions, namely tumor necrosis factor-alpha (TNF-α). However, the relationship between TNF-α -308G >A gene polymorphism (rs1800629) and the severity of Plasmodium falciparum (P. falciparum) malaria remains unclear, which prompts a meta-analysis to