Switchable and orthogonal gene expression control inside artificial cells by synthetic riboswitches

Yuta Ishii; Keisuke Fukunaga; Aileen Cooney; Yohei Yokobayashi; Tomoaki Matsuura

doi:10.1039/D4CC00965G

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Switchable and orthogonal gene expression control inside artificial cells by synthetic riboswitches†

Yuta Ishii,^ab Keisuke Fukunaga,

*^a Aileen Cooney,

^ac Yohei Yokobayashi

^d and Tomoaki Matsuura

*^a

Author affiliations

* Corresponding authors

^a Earth-Life Science Institute, Tokyo Institute of Technology, Ookayama 2-12-1, Meguro-Ku, Tokyo 152-8550, Japan
E-mail: keisuke.fukunaga@elsi.jp, matsuura_tomoaki@elsi.jp

^b School of Life Science and Technology, Tokyo Institute of Technology, Ookayama 2-12-1, Meguro-Ku, Tokyo 152-8550, Japan

^c Department of Chemistry, Molecular Sciences Research Hub, Imperial College London, London W12 0BZ, UK

^d Nucleic Acid Chemistry and Engineering Unit, Okinawa Institute of Science and Technology Graduate University, Onna, Okinawa 904-0495, Japan

Abstract

Here we report two novel synthetic riboswitches that respond to ASP2905 and theophylline and function in reconstituted cell-free protein synthesis (CFPS) system. We encapsulated the CFPS system as well as DNA-templated encoding reporter genes regulated by these orthogonal riboswitches inside liposomes, and achieved switchable and orthogonal control over gene expression by external stimulation with the cognate ligands.

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Article information

DOI: https://doi.org/10.1039/D4CC00965G
Article type: Communication
Submitted: 28 Feb 2024
Accepted: 12 May 2024
First published: 16 May 2024

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Chem. Commun., 2024, Advance Article

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Switchable and orthogonal gene expression control inside artificial cells by synthetic riboswitches

Y. Ishii, K. Fukunaga, A. Cooney, Y. Yokobayashi and T. Matsuura, Chem. Commun., 2024, Advance Article , DOI: 10.1039/D4CC00965G

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Chemical Communications

Switchable and orthogonal gene expression control inside artificial cells by synthetic riboswitches†

Abstract

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Switchable and orthogonal gene expression control inside artificial cells by synthetic riboswitches

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