B.S. degree in chemistry from University of Ioannina
Ph.D. in Biophysical Chemistry jointly from the University of Ioannina and Institute Curie
Structural & Mechanistic Biology, Protein Dynamics & Allostery
The main research interests of the lab have been focused on the elucidation of the molecular and mechanistic basis of a wide range of important biological phenomena. Our current and future efforts are directed towards understanding how central biological processes, such as transcription regulation, protein translocation and secretion, cell signaling, and protein folding take place. We strongly believe that a detailed characterization of biomolecular interactions necessarily involves investigation of the inter-relationship between function, structure, dynamics, kinetics and energetics of a system. Towards this challenging goal, we employ state-of-the-art NMR spectroscopy methodologies complemented by many other biochemical and biophysical techniques. NMR spectroscopy in particular is a very powerful methodology that allows the study of the three-dimensional structure of biomolecules in semi-physiological conditions. Furthermore, NMR spectroscopy can provide a unique insight into the dynamic aspects of the macromolecules and their interaction with ligands over time. Application of these methodologies in our lab has already resulted in very exciting and novel information.
T. Saio, X. Guan, P. Rossi, A. Economou & C.G. Kalodimos (2014) "Structural basis for protein anti-aggregation activity of the Trigger Factor chaperone" Science, 344, 1250494.
H-S. Tzeng & C.G. Kalodimos (2013) "Allosteric inhibition through suppression of transient conformational states" Nature Chemical Biology, 9, 462-465.
C.E. Tinberg, S.D. Khare, J. Dou , L. Doyle , J.W. Nelson, A. Schena , W. Jankowski, C.G. Kalodimos, K. Johnsson, B.L. Stoddard & D. Baker (2013) "Computational design of ligand-binding proteins with high affinity and selectivity" Nature, 501, 212-216.
L. Chen, X. Ai, A. Portaliou, D. Remeta, C. Menetti, A. Economou & C.G. Kalodimos (2013) "Substrate-activated conformational switch on chaperones encodes a targeting signal in type III secretion" Cell Reports, 3, 709-715.
H-S. Tzeng & C.G. Kalodimos (2012) "Protein activity regulation by conformational entropy" Nature, 488, 236-240.
W. Jankowski,T. Saleh, M.-T. Pai, S. Ganapathy, R. B. Birge & C.G. Kalodimos (2012) "Domain organization differences explain Bcr-Abl's preference for CrkL over CrkII" Nature Chemical Biology, 8, 590-596.
L. Chen, V. Balabanidou, D. Remeta, C. Menetti, A. Economou & C.G. Kalodimos (2011) "Structural instability tuning as a regulatory mechanism in protein-protein interactions" Molecular Cell, 44, 734-744.
P. Sarkar, T. Saleh, H-S. Tzeng, R.B. Birge & C.G. Kalodimos (2011) "Structural basis for regulation of the Crk signaling protein by a proline switch" Nature Chemical Biology, 7, 51-57.
H-S. Tzeng & C.G. Kalodimos (2009) "Dynamic activation of an allosteric regulatory protein" Nature, 462, 368-372.
G. Gouridis, S. Karamanou, I. Gelis, C.G. Kalodimos & A. Economou (2009) "Signal peptides are allosteric activators of the protein translocase" Nature, 462, 363-367.
N. Popovych, H-S. Tseng, M. Tonelli, R.H. Ebright & C.G. Kalodimos (2009) "Structural basis for cAMP-mediated allosteric transition in the catabolite activator protein" Proceedings of the National Academy of Sciences USA, 106, 6927-6932.
I. Gelis, A. Bonvin, D. Keramisanou, S. Karamanou, A. Economou & C.G. Kalodimos (2007) "Structural basis for signal sequence recognition by the 204-kDa SecA motor ATPase" Cell, 131, 756-769.
P. Sarkar, C. Reichman, T. Saleh, R.B. Birge & C.G. Kalodimos (2007) "Proline cis-trans isomerization controls autoinhibition of a signaling molecule" Molecular Cell, 25, 413-426.
N. Popovych, S. Sun, R.H. Ebright & C.G. Kalodimos (2006) "Dynamically driven protein allostery" Nature Structural & Molecular Biology, 13, 831-838.
D. Keramisanou, N. Biris, I. Gelis, S. Karamanou, G. Sianidis, A. Economou & C.G. Kalodimos (2006) "Disorder-order folding transitions underlie catalysis in the helicase motor of SecA" Nature Structural & Molecular Biology, 13, 594-602.
C. G. Kalodimos, N. Biris, A.M.J.J. Bonvin, M.M. Levandoski, M. Guennuegues, R. Boelens & R. Kaptein (2004) "Structure and flexibility adaptation between nonspecific and specific protein-DNA complexes" Science, 305, 386-389.
C. G. Kalodimos, R. Boelens & R. Kaptein (2004) "Toward an integrated model of protein-DNA recognition as inferred from NMR studies on the Lac repressor system" Chemical Reviews, 104, 3567-3586.
C. G. Kalodimos, A.M.J.J. Bonvin, R. K. Salinas, R. Weschelberger, R. Boelens & R. Kaptein (2002) "Plasticity in Protein-DNA recognition: lac Repressor Recognizes its Natural Operator with Alternative Conformations of its DNA-Binding Domain" The EMBO Journal, 21, 2866-2876.
C. G. Kalodimos, R. Boelens & R. Kaptein (2002) "A Residue-Specific View of the Association and Dissociation Pathway in Protein-DNA Recognition" Nature Structural Biology, 9, 193-197.