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个人简介

博士 教授 | 博士生导师 单位 化学系 1995年-2002年在东北大学化学系学习,获得理学学士和硕士学位。2003年考入清华大学化学系从事微流控芯片相关技术研究,2006年获得理学博士学位。后进入北京大学工学院从事博士后研究工作,进行小分子核酸分析和分子诊断技术研究。2008年加入浙江大学化学系任教至今,2012年至2013年获得日本学术振兴会奖学金在东京医科齿科大学做博士后,现已回国工作。目前研究方向主要是微流控芯片,定量PCR技术,单细胞分析和分子诊断新技术等。目前担任仪器分析和化学生物学实验主讲老师,发表文章30余篇。 教学工作 1. 分析化学乙 本课程主要包含化学分析和仪器分析内容;其中化学分析以介绍容量分析为主,包括酸碱、配位、氧化还原和沉淀滴定等容量分析内容,同时涉及重量分析;仪器分析适当介绍常见的电位法、紫外-可见分光光度法、红外光谱法、原子吸收分光光度法、色谱法和质谱法等。 2. 仪器分析 (本科生专业基础课程) 主要介绍电化学、紫外-可见吸收光度法、红外光谱、原子吸收光谱、色谱和质谱 3. 化学生物学实验 (面向高年级本科生) 负责其中三个实验:植物基因组DNA的提取与扩增;PCR-RFLP单核苷酸多态性分析;探究型实验:PCR技术鉴定转基因大豆 4. 生化分析/生命分析化学 (面向高年级本科生和研究生) 生化分析中常用生物技术;核酸分析;酶分析;蛋白质分析;免疫分析 5. 中级化学实验I 原子吸收光谱测定饮用水中鎂离子 6. Chem (Experiment) (A) 浙江大学国际联合学院(海宁) 项目支持 基于类器官芯片的肿瘤微环境及耐药研究 国家自然科学基金面上项目,22474125,主持 基于微流控的患者来源肿瘤细胞体外培养及药敏试验系统,国家自然科学基金面上项目,22174126,主持 微/纳流控-生物传感技术用于外泌体的分离分析,国家自然科学基金面上项目,21874116,主持 单细胞数字PCR技术用于肺癌循环肿瘤细胞的鉴定分析,国家自然科学基金面上项目,21375119,主持 微流控芯片单细胞microRNA表达分析研究 , 国家自然科学基金(青年),20905064,主持 微流控芯片高通量核酸序列依赖性扩增(NASBA)技术研究 浙江省自然科学基金,主持 微流控芯片高灵敏高通量microRNA检测方法的研究 教育部博士点新教师基金,主持

研究领域

· 核酸分析 · 生物传感 · 微流控分析

近期论文

查看导师新发文章 (温馨提示:请注意重名现象,建议点开原文通过作者单位确认)

2025 2. Yongan Ren, Ke Ge, Zhiying Yan, Xiaoxuan Liang, Qiaoqiao Tang, Yuanyu Wang, Bo Yao*, Kai Ye*, Min Wang*, Wenyu Zhu*, Multiplexed profiling of tumor-derived extracellular vesicles subpopulations via DNA flowers-assisted exponential amplification, Sensors and Actuators B: Chemical, 2025, 445(15): 138629 https://doi.org/10.1016/j.snb.2025.138629 1. Ziyang Zhou,Qiaoqiao Tang,Bo Yao* and Guangzhong Ma*, Advancing cancer diagnosis through surface plasmon resonance detection of extracellular vesicles, TrAC Trends in Analytical Chemistry, 2025, 118298 https://doi.org/10.1016/j.trac.2025.118298 2024 4. Yuanxin Zhang,† Zichen Hong,† Xuan Fu, Jiaying Li, Bo Yao,* and Guangzhong Ma*, Label-Free Impedance Imaging of Single Extracellular Vesicles Using Interferometric Electrochemical Microscopy, Anal. Chem. https://doi.org/10.1021/acs.analchem.4c04244 3. Tianyuan Fang, Xinlun Xie, Wei Lu, Zichen Hong, Wenbo Peng, Jun Zhou,* Min Wang,* and Bo Yao*, Patient-Derived Organoids on a Microarray for Drug Resistance Study in Breast Cancer, Anal. Chem. 2024, 96, 18384-18391 2. Ke Ge, Yongan Ren, Zichen Hong, Zhenjun Mao, Bo Yao*, Kai Ye*, Changku Jia*,Microchip Based Isolation and Drug Delivery of Patient-Derived Extracellular Vesicles Against Their Homologous Tumor,Adv. Healthcare Mater., 2024, 240199 1. Yongan Ren, Ke Ge, QiaoQiao Tang, Xiaoxuan Liang, Linlin Fan*, Kai Ye*, Min Wang*, and Bo Yao*, Dual-Recognition-Mediated Autocatalytic Amplification Assay for the Subpopulations of PD-L1 Positive Extracellular Vesicle, Anal. Chem. 2024, 96, 23, 9585–9592 2023 4. Yongan Ren, Ke Ge, Wei Lu, Xinlun Xie, Yang Lu*, Min Wang*, and Bo Yao*, Multivalent DNA Flowers for High-Performance Isolation, Detection, and Release of Tumor-Derived Extracellular Vesicles, ACS Appl. Mater. Interfaces, 2023, https://doi.org/10.1021/acsami.3c12211 3. Wenbo Peng,Danyang Sun, Wei Lu, Shenyi Yin, Buqing Ye, Xiaoqi Wang, Yongan Ren, Zichen Hong, Wenyu, Zhu*, Pengfei Yu*, Jianzhong Jeff Xi*, Bo Yao*, Comprehensive detection of PD-L1 protein and mRNA in tumor cells and extracellular vesicles through a real-time qPCR assay, Anal. Chem.,2023,95(28): 10625–10633 2. Jingfeng Zhang, Zichen Hong,Wei Lu,Tianyuan Fang,Yongan Ren,Shenyi Yin,Qijia Xuan,Dezhi Li*,Jianzhong Jeff Xi*, and Bo Yao*, Assessment of Drug Susceptibility for Patient-Derived Tumor Models through Lactate Biosensing and Machine Learning, ACS sensors, 2023, 8(2): 803–810 1. Yongan Ren, Ke Ge, Wei Lu, Wenbo Peng, Haiyan Che, Jianxin Ma*, Changku Jia*, Bo Yao*, Selective enrichment and detection of PD-L1 positive extracellular vesicles derived from human plasma and patient derived tumor cells, Sensors and Actuators B: Chemical, 2023, 377(15): 133086 2022 5. Tianyuan Fang,Wei Lu, Jingfeng Zhang, Ke Ge, Zhanhong Chen*, Min Wang*, Bo Yao*, Study of drug resistance in chemotherapy induced by extracellular vesicles on a microchip, Anal. Chem., DOI: 10.1021/acs. analchem. 2c04330 4. Bo Yao,Yunze Yang,Nanxi Yu,Nongjian Tao,Di Wang*,Shaopeng Wang*,Fenni Zhang*, Label-Free Quantification of Molecular Interaction in Live Red Blood Cells by Tracking Nanometer Scale Membrane Fluctuations,Small,2022, 2201623 3. Jiawei Shen, Tingting Li, Min Wang* and Bo Yao*, Isothermal and enzyme-free MicroRNA assay based on catalytic hairpin assembly and rare earth element labeled probes, Sensors and Actuators B: Chemical, 2022, 357: 131364 2. Bo Yao*,Danyang Sun,Yongan Ren,Min Wang,Introducing Theoretical Principles of Semi-, Relative, and Absolute Quantification via Conventional, Real-Time, and Digital PCR to Graduate and Senior Undergraduate Students of Chemistry,J. Chem. Educ. 2022, 99, 2, 603–611 1. Yongan Ren, Ke Ge, Danyang Sun, ZichenHong, Changku Jia, Huan Hu, Fangwei Shao, Bo Yao*, Rapid enrichment and sensitive detection of extracellular vesicles through measuring the phospholipids and transmembrane protein in a microfluidic chip, Biosensors and Bioelectronics, 2022, 199, 1: 113870 2019 3. Dai S, Lu W, Wang Y, Yao B*. Universal DNA biosensing based on instantaneously electrostatic attraction between hexaammineruthenium (III) and DNA molecules. Biosens Bioelectron. 2019 Feb 15;127:101-107. 2. YushengWang,XiaoqiWang,WeiLu,QingpanYuan,YourongZheng,Bo Yao*,A thin film polyethylene terephthalate (PET) electrochemical sensor for detection of glucose in sweat,Talanta,2019,198(1):86-92 1. Wei Lu, Caiqin Lin,Jun Yang,Xiaoqi Wang,Bo Yao*,Min Wang*,A DNAzyme assay coupled with effective magnetic separation and rolling circle amplification for detection of lead cations with a smartphone camera, Anal Bioanal Chem (2019) 411: 5383-5391 2018 Lu W, Wang Y, Song S, Chen C, Yao B*, Wang M*. A fishhook probe-based rolling circle amplification (FP-RCA) assay for efficient isolation and detection of microRNA without total RNA extraction. Analyst. 2018 Oct 8;143(20):5046-5053. 2017 Tabata M, Yao B, Seichi A, Suzuki K, Miyahara Y. Electrochemical Biosensors Combined with Isothermal Amplification for Quantitative Detection of Nucleic Acids. Methods Mol Biol. 2017;1572:135-151. Wei Lu, Qingpan Yuan, Zhiliu Yang, Bo Yao*, Self-Primed Isothermal Amplification for Genomic DNA Detection of Human Papillomavirus, Biosensors and Bioelectronics, 2017,15 April,90: 258-263 2016 Zhiliu Yang, YichenLiu, WeiLu, QingpanYuan, WeiWang, QiaoshengPu*,Bo Yao*, High specific surfacegoldelectrodeonpolystyrenesubstrate: CharacterizationandapplicationasDNAbiosensor, Talanta152(2016)301–307 Before 2015 Hua Wang, Weiwei Liu, Xinju zhang, Xiao Xu, Zhihua Kang, Shibao Li, Zhiyuan Wu, Zhiliu Yang, Bo Yao∗, Ming Guan*, Toward point-of-care testing for JAK2 V617F mutation on a microchip, Journal of Chromatography A, 1410 (2015) 28–34 Ying Zhu, Yun-Xia Zhang, Wen-Wen Liu, Yan Ma, Qun Fang* & Bo Yao*, Printing 2-Dimentional Droplet Array for Single-Cell Reverse Transcription Quantitative PCR Assay with a Microfluidic Robot, Scientific Reports, 2015, 5(9551): 1-7 Bo Yao*, Yichen Liu, Miyuki Tabata, Huangtianzhi Zhu and Yuji Miyahara*, sensitive detection of microRNA by chronocoulometry and rolling circle amplification on a gold electrode, Chem. Commun., 2014, 50, 9704-9706 Caiqin Lin, Yunxia Zhang, Xin Zhou, Bo Yao*, Qun Fang, Naked-eye Detection of Nucleic Acids through Rolling Circle Amplification and Magnetic Particle Mediated Aggregation, Biosensors and Bioelectronics, 2013, 47, 515-519 林彩琴,姚波*,数字PCR技术进展,化学进展,2012, 12,2415-2423 Yunxia Zhang, Ying Zhu, Bo Yao* and Qun Fang*, Nanolitre droplet array for real time reverse transcription polymerase chain reaction, Lab on a Chip, 2011, 11, 1545-1549 Yongqiang Cheng, Bo Yao, Huidan Zhang, Jin Fang and Qun Fang*, An automated capillary electrophoresis system for high-speed separation of DNA fragments based on a short capillary, Electrophoresis, 31, 19, 3184-3191, Oct, 2010 Jian-Zhang Pan, Bo Yao and Qun Fang, ,2010,82, 8, 3394-3398Hand-held Photometer Based on Liquid-Core Waveguide Absorption Detection for Nanoliter-scale Samples, Analytical Chemistry Bo Yao, Juan Li, Huang Huang, et al Quantitative analysis of zeptomole microRNAs based on isothermal ramification amplification, RNA, 2009, 15: 1787-1794 Juan Li, Bo Yao, Huang Huang, et al. Real-Time Polymerase Chain Reaction MIcroRNA Detection Based on Enzymatic Stem-Loop Probes Ligation, Analytical Chemistry, 2009, 81: 5446–5451 Bo Yao, Huihua Yang, Qionglin Liang, et al. High-speed, whole-column fluorescence imaging detection for isoelectric focusing on a microchip using an organic light emitting diode as light source. Analytical Chemistry, 2006, 78(6): 5845-5850 Bo Yao, Guoan Luo, et al. A Microfluidic Device using a Green Organic Light Emitting Diode as an Integrated Excitation Source, Lab on a Chip,2005, 5(10): 1041-1047. Bo Yao, Guoan Luo, et al. A microfluidic device based on gravity and electric force driving for flow cytometry and fluorescence activated cell sorting, Lab on a Chip, 2004, 4(6): 603~607 姚波,冯雪,罗国安,等,微流控芯片系统流式细胞术及单细胞荧光检测. 高等学校化学学报, 2005, 25(1): 43-45 姚波,颜流水,王义明,等,微全分析系统中的电色谱分离技术. 分析化学. 2004,32(12): 1673-1676 姚波、徐淑坤、方肇伦,流动注射在线吸着分离预浓集冷蒸气原子吸收法测定痕量镉,高等学校化学学报,2001, 22(8): 1301-1304

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