Molecular and clinical characterization of human adenovirus associated with acute respiratory tract infection in hospitalized children

https://doi.org/10.1016/j.jcv.2019.104254Get rights and content

Highlights

  • 3.71% of children with ARTI exhibited HAdV positive.

  • HAdV-2, HAdV-3 and HAdV-7 were the predominant types identified from ARTI children.

  • 74.85% of HAdV were co-detected with other respiratory pathogens, most commonly HRV.

  • The co-detection rate of HAdV-C was significant higher than those of HAdV-B.

  • HAdV-7 positive children may not present more severe clinical outcome.

Abstract

Background

Human adenovirus (HAdV) is a common pathogen in children that can cause acute respiratory tract infection (ARTI), but the molecular epidemiological and clinical information relating to HAdV among hospitalized children with ARTI are few reported in China.

Objectives

To evaluate the epidemiological, clinical, and molecular characteristics of HAdV infections among hospitalized children with ARTI in Hebei, Northern China from June 2017 to May 2018.

Study design

A 12-month longitudinal, retrospective study on HAdV, typed by nested polymerase chain reaction targeting the hexon gene’s hypervariable region (typing was merely performed by sequencing of the hexon neutralization epitope and thus genotypes could not be identified unequivocally), associated with ARTI was performed. The epidemiological and clinical data of different types of HAdV were analyzed using statistical product and service solutions (SPSS) 21.0 software.

Results

HAdV was detected in 330 (3.71%) of the 8906 specimens, with most (88.48%, 292/330) HAdV-positives cases detected among children < 3 years old. HAdV were detected throughout the year with a higher prevalence in spring. 11 types were identified, with HAdV-2 (33.33%, 110/330) as the predominant type, followed by HAdV-3 (21.21%, 70/330) and HAdV-7 (13.94%, 46/330). Of the 330 HAdV-positive specimens, 247 (74.85%) were co-detected with other respiratory pathogens, most commonly rhinovirus (HRV) (58.7%, 145/247). Additionally, patients with HAdV-7 positive had longer duration of fever than HAdV-2 or -3 positive patients.

Conclusions

During the study period, HAdV-2, HAdV-3 and HAdV-7 were the predominant types identified from children with ARTI in Hebei Province. Pediatric patients with HAdV-7 positive may not present more severe clinical outcome except a longer duration of fever.

Abbreviations

ARTI
acute respiratory tract infection
HAdV
Human adenovirus
CAP
community-acquired pneumonia
NPA
nasopharyngeal aspirate
FluA
Influenza A
FluB
Influenza B
09H1
Influenza A H1N1 pdm09
H3
Influenza H3N2
HPIV
Human parainfluenza virus
RSV
Respiratory syncytial virus
HMPV
Human metapneumovirus
HRV
Rhinovirus
HBoV
Human bocavirus
HCoV
Human coronavirus
Ch
Chlamydia
Mp
Mycoplasma pneumoniae

Keywords

Adenovirus
Acute respiratory tract infection
Molecular epidemiology
Clinical characterization
Children

Cited by (0)

1

Meng-chuan Zhao, Ying-hui Guo and Fang-zhou Qiu contributed equally to the article.

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