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Characteristics of an Acidic Phytase from Aspergillus aculeatus APF1 for Dephytinization of Biofortified Wheat Genotypes

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Abstract

Phytases are the special class of enzymes which have excellent application potential for enhancing the quality of food by decreasing its inherent anti-nutrient components. In current study, a protease-resistant, acidic phytase from Aspergillus aculeatus APF1 was partially purified by ammonium sulfate fractionation followed by chromatography techniques. The molecular weight of partially purified phytase was in range of 25–35 kDa. The purified APF1 phytase was biochemically characterized and found catalytically active at pH 3.0 and 50 °C. The Km and Vmax values of APF1 phytase for calcium phytate were 3.21 mM and 3.78 U/mg protein, respectively. Variable activity was observed with metal ions and among inhibitors, chaotropic agents and organic solvents; phenyl glyoxal, potassium iodide, and butanol inhibited enzyme activity, respectively, while the enzyme activity was not majorly influenced by EDTA, urea, ethanol, and hexane. APF1 phytase treatment was found effective in dephytinization of flour biofortified wheat genotypes. Maximum decrease in phytic acid content was noticed in genotype MB-16-1-4 (89.98%) followed by PRH3–30-3 (82.32%) and PRH3–43-1 (81.47%). Overall, the study revealed that phytase from Aspergillus aculeatus APF1 could be effectively used in food and feed processing industry for enhancing nutritional value of food.

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Funding

Financial support was provided by the Department of Biotechnology (DBT), Govt. of India (Grant No. BT/AGR/BIOFORTI/PHII/NIN/2011) to carry out this work; Ministry of Food Processing Industries (MoFPI), Govt. of India, provided “infrastructural facility development” at Eternal University; and Head, Department of Microbiology, Maharshi Dayanand University, Rohtak permitted leading author to carry out part of this work at the Department.

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  1. Department of Biotechnology, Eternal University, Baru Sahib, 173101, India

    Abhishake Saxena, Meena Verma, Ajar Nath Yadav, Harcharan Singh Dhaliwal & Vinod Kumar

  2. Department of Biotechnology, Central University of Haryana, Jant-Pali, Mahendragarh, 123031, India

    Bijender Singh

  3. Department of Biochemistry, Eternal University, Baru Sahib, 173101, India

    Punesh Sangwan

  4. Quality Analysis Laboratory, Forage Section, College of Agriculture, CCS Haryana Agricultural University, Hisar, 125004, India

    Vinod Kumar

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  1. Abhishake Saxena
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Saxena, A., Verma, M., Singh, B. et al. Characteristics of an Acidic Phytase from Aspergillus aculeatus APF1 for Dephytinization of Biofortified Wheat Genotypes. Appl Biochem Biotechnol 191, 679–694 (2020). https://doi.org/10.1007/s12010-019-03205-9

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