Anti-aging effect of brown black wolfberry on Drosophila melanogaster and d-galactose-induced aging mice

https://doi.org/10.1016/j.jff.2019.103724Get rights and content
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Highlights

  • The anti-aging effect of BBW prepared by the Maillard reaction was studied.

  • BBW could prolong the lifespan of Drosophila melanogaster.

  • BBW could improve anti-aging ability of aging mice.

  • BBW could regulate the mRNA expression levels of Drosophila melanogaster and aging mice.

Abstract

Aging is a global challenge. Many studies reported that the accumulation of ROS and oxidative stress could be the most important causative factors for aging. Phenolic, flavonoids, and melanoidins possess potent of ROS scavenging ability. In this study, brown black wolfberry (BBW) was prepared via the Maillard Reaction (MR) and its anti-aging potential was explored using the Drosophila melanogaster and d-gal induced aging mice as the animal model. Results showed that total phenolic and flavonoid contents and browning index were increased after MR. In vivo study results indicated that BBW significantly increased the survival time and alleviated the oxidative stress caused by H2O2 of Drosophila melanogaster. Meanwhile, BBW significantly ameliorated oxidative damage and d-gal-induced aging in mice and inhibited lipid peroxidation by enhancing the anti-oxidant enzyme activities and up-regulating the expressions of the endogenous stress defense genes (SOD1, GSH-Px1, GSH-Px2 of aging mice and SOD1, SOD2, CAT of Drosophila melanogaster).

Keywords

Brown black wolfberry
Maillard reaction
Anti-ageing
d-Galactose
Drosophila melanogaster

Abbreviations

BBW
brown black wolfberry
RW
red wolfberry
SOD
superoxide dismutase
GSH-Px
glutathione peroxidase
CAT
catalase
T-AOC
total anti-oxidant capacity
MDA
malondialdehyde
GAPDH
glyceraldehyde-3-phosphate dehydrogenase
SOD1
CuZn-superoxide dismutase
GSH-Px1
glutathione peroxidase 1
GSH-Px2
glutathione peroxidase 2
d-galactose
d-gal
H2O2
hydrogen peroxide
RT-PCR
real-time polymerase chain reaction
ROS
reactive oxygen species
MR
maillard reaction
MRPs
maillard reaction products

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