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Fluorescence detection of laccases activity by the photoinduced electron transfer (PET) process

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Abstract

Laccases play a vital role in some physiological processes, for example in morphogenesis, carbon cycle, and defense against parasitism. So, designing a high-sensitivity accurate method is essential for researchers. In this study, a simple fluorescence method based on the function of carbon nitride (g-C3N4) by dopamine is synthesized. For the design of this sensor, carbon nitride (g-C3N4) is initially synthesis by using a simple method, which is carried out by heating melamine at 550 °C for 3 h and modifying it with dopamine by a linker such as glutaraldehyde. However, the g-C3N4–Dopa produced by this method, with an excitation wavelength of 330 nm, has a fluorescence emission at 466 nm. When laccase and g-C3N4–Dopa were mixed, dopamine with redox property was oxidized to dopaquinone; this causes the phenomenon of photoinduced electron transfer (PET) process between g-C3N4 and the dopaquinone. Hence, fluorescence quenching occurs due to this phenomenon. As a result of these discussions, a sensor for the laccase activity was designed based on the fluorescence quenching degree, supporting a linear range of 0.0–400.0 U L−1 with the detection limit of 2.0 U L−1. Using this sensor, the activity of the laccase enzyme in the human serum samples is measured.

Graphic abstract

Dopamine-functionalized carbon nitride was prepared and utilized for the highly sensitive detection of laccases activity.

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  • 17 December 2020

    The original article can be found online.

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Acknowledgements

The authors gratefully acknowledge the Medical Cellular and Molecular Research Center of Golestan University of Medical Sciences (Grant Number: 111255) for the financial support.

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Authors and Affiliations

  1. Medical Cellular and Molecular Research Center, Golestan University of Medical Sciences, Gorgān, Iran

    Mehdi Sheikh Arabi

  2. Nano Drug Delivery Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Changiz Karami

  3. Department of Chemistry, Faculty of Sciences, Shahid Bahonar University of Kerman, Kerman, Iran

    Changiz Karami & Mohammad Ali Taher

  4. Department of Chemistry, Islamic Azad University, Kermanshah Branch, Kermanshah, Iran

    Elahe Ahmadi

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  1. Mehdi Sheikh Arabi
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Correspondence to Changiz Karami.

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Arabi, M.S., Karami, C., Taher, M.A. et al. Fluorescence detection of laccases activity by the photoinduced electron transfer (PET) process. J Biol Inorg Chem 25, 151–159 (2020). https://doi.org/10.1007/s00775-019-01748-0

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