Abstract
Specific enrichment and efficient detection of phosphopeptides are important for phosphoproteomic analysis. Metal–organic frameworks (MOFs) have been proved to be effective enrichment materials combined with the analysis of mass spectrometry. In this work, we have developed a polycarboxylic group MOF-UiO-66-(COOH)2 used as both efficient enrichment material for phosphopeptides and efficient desorption/ionization matrix of laser desorption/ionization time-of-flight mass spectrometry (LDI-TOF MS) without elution. The crystal is spherical and the particle size is about 200 nm. When using it as enrichment material and MS matrix, phosphopeptides of β-casein can be efficiently detected under superior low detection limits of 1 fmol μL−1, and at an extremely low molar ratio of phosphoprotein/non-phosphoprotein (1:2000) mixtures, there are still four phosphopeptides that can be observed. When detecting real samples, most phosphopeptides of non-fat milk and human saliva can be observed, which is comparable with the literature.
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The authors received financial support from National Natural Science Foundation of China (21874073).
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This article is part of the topical collection Nanoparticles in Biotechnology and Medicine
Xiaoshan (Sean) Zhu, University of Nevada, Guest Editor
Highlights
• Polycarboxyl metal–organic framework UiO-66-(COOH)2 was used as matrix of laser desorption/ ionization time-of-flight mass spectrometry and adsorbent of phosphopeptides at the same time.
• Phosphopeptides were detected easily with UiO-66-(COOH)2 at a low detection limit of 1 fmol/μL.
• UiO-66-(COOH)2 showed high selectivity when detecting complex samples.
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Yang, Y., Xia, Y. Polycarboxyl metal–organic framework UiO-66-(COOH)2 as efficient desorption/ionization matrix of laser desorption/ionization mass spectrometry for selective enrichment and detection of phosphopeptides. J Nanopart Res 21, 252 (2019). https://doi.org/10.1007/s11051-019-4646-7
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DOI: https://doi.org/10.1007/s11051-019-4646-7